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Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle
Get PDFMyostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P≤0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P≤0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality
Pengaruh pendedahan buku bergambar tentang cara memelihara domba pada peningkatan pengetahuan peternak domba Garut didesa Ciomas dan Kawung Girang kabupaten Majalengka Jawa Barat
No full textPengaruh pendedahan buku bergambar tentang cara memelihara domba pada peningkatan pengetahuan peternak domba Garut didesa Ciomas dan Kawung Girang kabupaten Majalengka Jawa Bara
Analisis ekonomi usaha ternak sapi perah di wilayah Propinsi Jawa Barat
No full textAnalisis ekonomi usaha ternak sapi perah di wilayah Propinsi Jawa Bara
Model usaha tani integrasi tanaman-ternak pada lahan sawah irigasi
No full textModel usaha tani integrasi tanaman-ternak pada lahan sawah irigas
Socio economi characteristics as determinants of cattle farmer information exposured (A Case Study in Sundamekar, Sumedang)
No full textSocio economi characteristics as determinants of cattle farmer information exposured (A Case Study in Sundamekar, Sumedang
Conception Rates of Holstein-Friesian Cows Inseminated Artificially with Reducing Frozen Semen Doses
Get PDFConception rates of Holstein Friesian (HF) acceptors were evaluated in artificial insemination (AI) mating using three frozen semen doses of the straws per volume of 0.25 cc. Frozen semen consisted of a standard dose by 25 x 106 sperms (L.1) and two reduced doses by 20 x 106 sperms (L.2) and 15x106 sperms (L.3). This field study was conducted in dairy cattle area of Lembang NBMCU, from August 2011 to March 2012. Frozen semens were obtained from two HF national progeny tested young bulls. Conception was detected by rectal palpation, around 60-90 days after AI mating. Conception rates (CR %) were calculated as a ratio between pregnant acceptors to the total AI mated cows. Some general linear model analyzes were conducted by considering fixed variables of semen dose (3), bull (2), inseminator (6) and parity (5). The results showed that three frozen semen doses did not significantly affect (P>0.05) on CR (%). By decreasing semen dose to L.3 gave CR (%) similar to L2 even to L1. It was concluded that frozen semen doses of HF bulls at the levels of 20 million to 15 million sperms per straw per volume of 0.25 cc resulted CR (%) similar to the standard one
Pengembangan Model Fuzzy Berlian Keunggulan Kompetitif pada Indrustri Penggolaha Susu di Indonesia
No full textPengembangan model Fuzzy berlian keunggulan kompetitif pada indrustri penggolaha susu di Indonesi
Penambahan Lysin dan Methionin pada Dedak untuk Pakan Entok yang Sedang Tumbuh
No full textPenambahan Lysin dan Methioninpada Dedak untuk Pakan Entok yang Sedang Tumbu
Perbandingan Perilaku Komunikasi Peternak di Desa Urban dan Desa Rural
No full textPerbandingan Perilaku Komunikasi Peternak di Desa Urban dan Desa Rura