Majalah Kedokteran Gigi Indonesia
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Vitamin D inhibits TNF-α serum level in wistar rats stimulated with Porphyromonas gingivalis
No full textPeriodontitis is a chronic inflammation of the periodontal tissues initiated by increased proinflammatory cytokines such as TNF-α due to the induction of Porphyromonas gingivalis (Pg). This study aimed to analyze the effect of vitamin D (cholecalciferol) on TNF-α serum level in Wistar rats stimulated with Pg to induce periodontitis. Twenty-seven male Wistar rats (n = 27) were divided into three equal groups. Group I was healthy Wistar rats that received 2000 IU vitamin D once a day. Group II was Wistar rats stimulated with Pg and received 2000 IU vitamin D, while group III was Wistar rats stimulated with Pg but did not receive vitamin D. Blood was collected through the orbital sinus and centrifuged to get the serum. TNF-α serum levels were assessed using Elisa method on the 7th, 14th, and 28th. The data were normally distributed and homogeneous. The mean TNF-α data was analyzed for differences between groups using the one-way ANOVA and LSD post hoc test. Significant differences were seen in group II (418.49 ± 161.08 ng/mL) and group III (172.16 ± 104.18 ng/mL) on the 28th day (p = 0.001). The findings suggest that vitamin D inhibits the TNF-α serum level in Wistar rats stimulated with Pg on the 28th day
Differences in mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis
No full textBruxism is a condition that involves grinding and clenching which can place a large load on the mandible, so it can change the morphology of the mandible. Based on the Levandoski analysis, this study aimed to determine whether there are differences in the mandibular morphology between bruxism and non-bruxism patients. This study was a cross-sectional analytic study conducted on the panoramic radiographic samples of bruxism and non-bruxism patients with a total sample of 30 patients (n = 30) in each group, obtained from the radiographic archives at the Radiology Installation of RSGM Universitas Padjadjaran which have been confirmed as bruxism and non-bruxism patients. Measurements were carried out using ImageJ software based on the reference lines by Levandoski. The results of the study were analyzed using SPSS software by performing normality and homogeneity tests, followed by a statistically independent t-test or Mann-Whitney test. The results of the independent t-test showed that three lines had a p-value 0.05. There were differences in the mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis, namely the length of the maxillary vertical midline, the distance from the condyle top point to the maxillary vertical midline, the distance from the point gonion to the maxillary vertical midline, the distance from the top point of the condyle to the maxillary interincisal point on the left side, and the distance from the top point of the condyle to the mandibular interincisal point on the left side. On the other four lines, there was no significant difference between bruxism and non-bruxism patients
The effect of various denture cleansers on porosity and discoloration level of acetal resin denture base
No full textDenture cleansing is one clinical parameter of successful denture wearing. This study aims to examine the effect of the type of denture cleanser on porosity and discoloration of acetal resin as a denture base. Twenty-four samples of acetal resin with 10 mm in diameter and 2 mm in thickness were divided into four groups. Porosity was measured using the wet and dry sample measurement method, and color value levels were measured using chroma meter for all samples. All of the samples were soaked into four types of denture cleanser: distilled water, alkaline peroxide tablets, 0.2% chlorhexidine gluconate, and 0.5% sodium hypochlorite for seven days, then the final porosity and color value level were assessed. The results showed that there were significant differences between groups in porosity and in color change of acetal resin (p < 0.05). Pearson’s correlation test showed that there was a positive correlation between the porosity level and discoloration level of acetal resin (p < 0.05). The research discovered that the type of denture cleaner has a significant influence on the porosity and color change of acetal resin. Sodium hypochlorite has the highest porosity and color change when compared to other cleansing agents
Effect of hexane extract of Clinacanthus nutans leaves on HSC-3 cells migration in vitro
No full textCancer is a major health issue and considered the most life-threatening disease worldwide. The oral cavity is one of the top ten locations in the body most frequently affected by cancer. The most common type of oral cancer (90%) is squamous cell carcinoma. Leaves of C. nutans have many uses and benefits, one of which is its potential as an anticancer agent. This study aims to determine the effect of C. nutans leaves hexane extract on HSC-3 cell strain migration. The leaves of C. nutans were extracted using a hexane solvent with the maceration method. Various concentrations (500, 250, 100, 50 and 25 mg/mL) were assessed to identify the effect of C. nutans leaves hexane extract on HSC-3 cells migration at 0, 6, 12, 24 and 48 time point. The concentration of 500 μg/mL was found to possess the ability to inhibit migration and induce apoptosis by the 6th hour. The concentration of 250 μg/mL induced apoptosis by the 24th hour. Concentrations of 100 μg/mL and 50 μg/mL were capable of inhibiting cell migration, and the concentration of 25 μg/mL could only delay cell migration up to the 12th hour. In conclusion, hexane extract of C. nutans leaves can inhibit HSC-3 cells migration
Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis
No full textSodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis
Treatment of temporomandibular joint trauma following odontectomy
No full textTemporomandibular Joint Disorder (TMD) is a non-dental musculoskeletal disorder that has a complex and multifactorial etiology. The most frequent symptoms are pain in the masticatory muscles, in front of the ears, and in the temporomandibular joint (TMJ); this disorder can also be accompanied by clicking sounds and locking. This study aimed to present a case report of temporomandibular joint trauma following odontectomy. A 22-year-old patient came with some complaints, namely pain in the TMJ, a clicking sound when opening and closing the mouth, and limited mouth opening. The symptoms started when the patient underwent odontectomy on all the four third molars, causing tightness around the jaw, pain, and clicking. However, she did not seek any treatment for these conditions until the pain became worse and disturbed her activities. The patient had to deal with limited mouth opening and severe migraines up to twice a day. During an examination, the patient was able to open her mouth without pain only 25 mm in width, with a maximum mouth opening of 35 mm. The therapy was done by constructing a 4-mm stabilization splint and educating the patient on how to reduce the pain. In fact, 2 months after the treatment and the use of stabilization splints, her complaints of TMJ pain and clicking sounds decreased, her mouth opening increased, and the migraines never came back. In this case, non-invasive treatment as the first-line therapy in patients with disc displacement with reduction and arthralgia can reduce the joint pain symptoms, and some of the symptoms have even disappeared
Evaluation of viscosity and pH of Zingiber officinale var. officinale juice in mouthwash formulation
No full textZingiber officinale var. officinale juice has many benefits, including as a mixture of mouthwash. This research aims to analyze the differences in pH and viscosity values of Zingiber officinale var. officinale-containing mouthwashes with concentrations of 3.125%, 6.25%, and 12.5% on days 0, 14, 28, 42, and 56. Zingiber officinale var. officinale-containing mouthwashes with concentrations of 3.125%, 6.25%, and 12.5% were mixed with propylene glycol, PEG-40 hydrogenated castor oil, oleum menthae, benzoic acid, sodium benzoate, calcium lactate, sorbitol 70% and calcium thiocyanate. The formulation was measured with a pH meter (Jenway, United Kingdom) and a viscometer (Brookfield, Massachusetts) for 56 days. pH data was analyzed using GLM repeated measures ANOVA and one-way ANOVA (p < 0.05). Viscosity data was analyzed using GLM repeated measured ANOVA and the Kruskal-Wallis test (p < 0.05). The pH value of the 3.125% formulation showed significant difference for 56 days in the range of 5.50-5.61. The viscosity value showed no significant difference for 56 days in the range of 3.03-3.09 cP. The pH and viscosity value of the 6.25% formulation showed no significant difference for 56 days with pHs of 5.78-5.83, while the viscosity is 3.12-3.13 cP. The pH and viscosity value of the 12.5% formulation showed no significant difference for 56 days with pHs of 4.90, while the viscosity was 3.43-3.50 cP. The Zingiber officinale var. Officinale mouthwash formulation with a concentration of 3.125% was unstable, but it had the lowest viscosity value and was stable for 56 days. The pH and viscosity value of the 6.25% and 12.5% formulations were stable for 56 days. The highest pH value was in the 6.25% formulation
Candida albicans adherence on soft denture liner coated with silica and titanium nanoparticles
No full textThe soft liner of acrylic resin dentures, used to coat removable partial dentures, still has drawbacks in the easy attachment of Candida albicans fungus. A treatment on the surface of the acrylic resin denture soft liner is needed to reduce the attachment of Candida albicans. Silicon dioxide (SiO2) and titanium dioxide (TiO2) nanoparticles have antimicrobial properties because they can induce photocatalytic production. This study aims to determine the effect of silica and titanium coating concentration on the attachment of Candida albicans to the soft liner of an acrylic resin denture. This study used 48 samples for 2 types of research. Each research consisted of 4 groups with 6 samples each. The samples were disc-shaped, with a diameter of 10 mm and a thickness of 2 mm. Silica and titanium coating materials of 0.5%, 1%, and 2% were obtained by mixing each nanoparticles of 0.5 g, 1 g, and 2 g in 100 ml of ethanol. After the application of the coating, the attachment test of Candida albicans was carried out. Candida albicans in SDA media were counted using a colony counter. The analysis was carried out using a one-way ANOVA test for each experiment. The ANOVA results showed an effect of silica and titanium coating concentrations on the attachment of Candida albicans to the soft liner of an acrylic resin denture (F = 10.929; p < 0.05 for silica, and F = 9.830; p < 0.05 for titanium). The group with a 2% silica coating concentration had the least amount of Candida albicans among all groups (0.48 ± 0.98 x 107 CFU/ml), as well as the group with a 2% titanium coating concentration (0.30 ± 0.83 x 107 CFU/ml). In conclusion, this study shows that the concentration of silica and the 2% titanium coating is the most effective in preventing the attachment of Candida albicans to the soft liner of acrylic resin dentures
Relationship between dental caries level and quality of life of communities aged 35-44 years in South Daha District, South Hulu Sungai Regency
No full textDental caries is the most common dental and oral health problem worldwide, including in Indonesia. Dental caries can be caused by a person’s decreased level of productivity, and severe dental caries can cause pain and difficulty eating and sleeping, thus affecting the quality of life. This study aims to determine the relationship between the level of dental caries and the quality of life of people aged 35-44 years in Daha Selatan District, Hulu Sungai Selatan Regency, South Kalimantan Province. This research used analytic observational method with a cross sectional approach. The population of this research was 106 people of South Daha District aged 35-44 years who were selected using simple random sampling technique. The results of statistical analysis using the Chi-square test obtained a significance value of 0.012 (p < 0.05), indicating a significant correlation between the level of dental caries and the quality of life of 35-44-year-olds. There is a definite need for increasing public awareness of the importance of maintaining oral and dental health to reduce cases of tooth decay caused by dental caries and improve people’squality of life
The relationship between caries risk assessment using the CAT-AAPD method and the incidence of caries in children
No full textSouth Kalimantan Province has a fairly high dental caries problems, and one region with this problem is South Hulu Sungai Regency with a percentage of cases of 45.56%. Dental caries is influenced by various risk factors, including socioeconomic status, eating habits, general health, fluorine use, saliva, and caries experience. Risk assessment of dental caries can be done using the Caries-Risk Assessment Tool by the America Academy of Pediatric Dentistry (CAT-AAPD). This study aims to analyze the relationship between caries risk assessment using the CAT-AAPD method and the incidence of caries in children aged 6-8 years in South Daha District, South Hulu Sungai Regency. This is an observational analytic study with a cross-sectional approach. The population of this study was children in South Daha aged 6-8 years. The total number of samples was 104 selected using the simple random sampling technique. High category caries risk was most common in children suffering from dental caries in a very high category with 64 respondents (53.3%). The results showed that there was a strong relationship (< 0.05) between caries risk assessment using the CAT-AAPD method and the incidence of caries in children aged 6-8 years. The higher the risk of caries, the higher the incidence of dental caries. Parents’ increased awareness of maintaining children’s dental and oral health is important to reduce cases of tooth decay caused by dental caries, and they can determine the treatments that should be undertaken