Majalah Kedokteran Gigi Indonesia
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Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats
Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing
Development of dentists perceptions and knowledge of teledentistry
Teledentistry is a dental health service that provides consultation, guidance, or education remotely using information technology. Perception and knowledge play an essential role in the implementation of teledentistry. The purpose of this study was to explore the development of dentists' perceptions and knowledge of the concept of teledentistry. This study used a scoping review method based on the Joanna Briggs Institute methodology. Screening and study selection were carried out using the Preferred Reporting Items of Systematic Reviews and Meta-Analysis Scoping Reviews (PRISMA-Scr). Literature searches were conducted through PubMed, Science Direct, and EBSCOHost with keywords and inclusion criteria, hand searching, and viewing the list of selected article references. There were 23 articles analyzed according to the inclusion and exclusion criteria. Changes in dentists' perceptions of teledentistry would help shorten waiting list, reduce costs for dental practice, additional visits to take photographs, and additional costs to provide facilities/ equipment for teledentistry before and during the COVID-19 pandemic. Three articles (2015-2016) state that most dentists did not know or have little knowledge about teledentistry. Nine articles published during the pandemic revealed that the majority of dentists had knowledge of teledentistry. The results of this study found that developments in dentists’ perception, namely teledentistry would help shorten waiting list, reduce costs for dental practice, the need for additional visits to take photographs, and additional costs to provide facilities/ equipment for teledentistry. The level of knowledge of dentists about teledentistry increased during the COVID-19 pandemic
Differences in levels of bone morphogenetic protein-2 in periodontitis patients with and without type 2 diabetes mellitus
Periodontitis is characterized by gingival bleeding, periodontal pocket formation, damage to the connective tissue attachment, and alveolar bone resorption. One of the risk factors that can increase the occurrence of periodontitis is diabetes mellitus (DM). In Indonesia, the incidence and prevalence of periodontitis are higher in patients with type 2 DM. In the present study, BMP-2 expression in periodontitis patients with and without type 2 DM was evaluated. We performed a descriptive analytical cross-sectional study in 40 respondents with stages II-III grades B-C chronic periodontitis. They were divided into a chronic periodontitis group with type 2 DM and a chronic periodontitis group without DM. Crevicular fluid of the gingival sulcus of the central maxillary anterior teeth from each respondent was taken using paper points for 5 minutes and placed in an Eppendorf tube. Each tube was labelled according to its group. All of the samples were examined by BMP-2 ELISA kit and measured by BioRad microplate reader with a wavelength of 450 nm. Data were analysed using SPSS 16.0 program. The independent sample t-test was used to compare the BMP-2 among two groups. The results of the study revealed the BMP-2 level in periodontitis patients with type 2 DM was 63.1 pg/ml, while in periodontitis patients without DM was 66.8 pg/ml. BMP-2 levels in periodontitis patients with type 2 DM were significantly lower than those in periodontitis patients without DM (p < 0.05)
Retrieval of broken file and retreatment of previously treated mandibular left second molar
Broken files in the root canal system which are not removed may interfere with thorough cleaning of the root canals. As a result, they cannot be hermetically obturated. This imperfect cleaning can leave necrotic tissue which can potentially cause failure in root canal treatment. The purpose of this paper is to describe the management of retrieval of broken files and re-treating root canals in previously treated mandibular left second molars. The retrieval of the broken file used conventional techniques combined with a Satelec ultrasonic scaler to remove the files from the root canal. It was operated under an endodontic microscope, and after retreatment of the root canal, the tooth was restored with final restoration of endocrown. Retrieval of fractured files using conventional techniques combined with Satelec ultrasonic scaler and the root canal re-treatment followed by final endocrown restoration could restore all four tooth functions properly
Effect of force direction and masticatory force towards orthodontic tooth movement in rats
The aim of the research is to investigate the influence of coil spring directions and masticatory force on the amount of OTM. Materials and Methods. Thirty-six male Wistar rats (n = 36) were divided proportionally into two groups with (M) or without masticatory force (NM), treated with palatal coil type (PD) or labial coil (LD) using a costumed stainless steel coil spring to deliver 35 cN force for separating the two incisors in 10 days. The examination dates were day 0, day 5, and day 10. The tooth distance values were calculated by subtracting the distance measured at day 0 from examination days and presented in 8 groups: PD5NM, PD10NM, PD5M, PD10M, LD5NM, LD10NM, LD5M, and LD10M. The study’s results were analyzed using ANOVA followed by post hoc analyses. Result: All spring designs induced OTM. The OTM amounts from the lowest to the highest are PD5M, PD10NM, PD10M, LD5M, LD10M, LD5NM, PD5NM, and LD10NM, respectively: 0.26 mm; 0.06 mm; 0.25 mm; 0.44 mm; 0.58 mm; 0.9 mm; 0.97 mm; 1.03 mm, and 1.06 mm. The OTM distance was higher in the labial coil than in the palatal coil groups (p = 0.002). The amount of OTM in the masticatory group was lower than in the group without-masticatory force (p = 0.012), except in the day 10 palatal coil group. Conclusions: Masticatory force and force direction affected the amount of OTM. The labial coil induces more OTM than the palatal coil. Masticatory force decreased the OTM distance
Uremic stomatitis mimicking oral squamous cell carcinoma associated with local and systemic factor
Uremic stomatitis is one of the oral manifestations of chronic kidney disease. This disease is caused due to increased levels of urea in the blood circulation. Patients will complain sore lesions accompanied by a characteristic ammonia breath odor. The clinical manifestation of uremic stomatitis is not only influenced by high levels of urea in the blood but also related to local factors, the oral anatomy and parafunction habits. This study aimed to present a case report of clinical features of uremic stomatitis that mimicking oral squamous cell carcinoma. A 51 year old woman was referred from the department of internal medicine to the department of oral medicine at Hasan Sadikin Hospital, Bandung. The patient complained of pain in the tongue and also when swallowing. The clinical manifestation shows an ulcerated lesion on the right lateral of the tongue and a slightly raised hyperkeratotic area on the floor of the mouth. The treatment is using 0.12% chlorhexidine digluconate mouthwash three times a day and followed by applying a thin layer of vaseline on the upper and lower lips, as well as instructions and education on maintaining oral and tongue hygiene. Controls were carried out on the second and fourteenth day. The patient complaints are decreasing until disappear. The patient's masticatory function is getting better. Local and systemic factors have effect on the clinical manifestation of uremic stomatitis. A clinician must be able to carry out examinations, establish diagnosis and appropriate management so that patients receive appropriate treatment
Anti-inflammatory loading of cinnamaldehyde on artificial bone scaffolds against the process of bone regeneration
Cinnamaldehyde is an organic component in cinnamon and has anti-inflammatory, antibacterial, and osteogenic properties. Loading cinnamaldehyde with a concentration of 4% into an artificial scaffold from gypsum calcium carbonate hydrogel could reduce inflammation, accelerate healing, and promote new bone regeneration at bone implantation surgery. The aim of this study was to investigate the impact of cinnamaldehyde loaded on gypsum-hydrogel calcium carbonate on the process of wound healing and bone regeneration in rats. Implantation of bone scaffolding from gypsum hydrogel calcium carbonate with an addition of cinnamaldehyde was performed on 20 5-month-old Sprague-Dawley rats weighing 300-350 g in an artificial defect of condyle femoris bone dexter and sinister. Group A was a control with gypsum only, while groups B, C, and D were given gypsum and CaCO3 with a combination of cinnamaldehyde or dehydrothermal treatment (DHT). Euthanasia was performed after implantation at 1, 4 and 8 weeks for 4 groups (n = 3). Femoris condyle bone was cut, made into histological preparations by hematoxylin eosin (HE) staining, and analyzed descriptively. The results showed that the loading of cinnamaldehyde on the scaffold was effective in weeks 1 and 4, but after 8 weeks of implantation, cinnamaldehyde was observed to inhibit defect closure. The cinnamaldehyde group combined with dehydrothermal (DHT) treatment was found to be better than those without DHT
Relationship between peatland water use and periodontal disease in Daha Selatan Subdistrict
Periodontal disease is one of the dental and oral health problems in Hulu Sungai Selatan Regency with a prevalence of gingivitis of 24.58% and periodontitis of 10.83%. One of the risk factors for periodontal disease is the use of peatland water. Peatland water has a low pH which can affect the colony of anaerobic bacteria in the oral cavity of the water users. Bacteria that cause periodontal disease are able to develop and survive in acidic conditions. This study aimed to analyze the relationship between peatland water use and periodontal disease in Daha SelatanSubdistrict. This was analytical research with a cross-sectional approach. The sampling technique used a simple random sampling technique with a total sample of 100 people (n = 100) who were residents aged 35-44 years in Daha Selatan Subdistrict. The results showed that the participants with high use of peatland water showedthe highest percentage (39%). In addition, there were 61% respondents who suffered from periodontal disease. ChiSquare test obtained a significance value of 0.022 (p < 0.05), showing that there was a relationship between peatland water use and periodontal disease. The risk of periodontal disease in the respondents who used peatland water in the high category was 4.167 times greater than in the respondents who did not use peatland water. It is essential to educate the general public about the effects that using peatland water can have on dental and oral health because there is a high prevalence of periodontal disease, which is caused by the widespread use of peatland water
Management of herpangina: a viral infection in infants
Herpangina is a self-limiting disease caused by coxsackievirus. The manifestation consists of acute febrile with small ulcerative or vesicular lesions on the tonsillar, soft palate, uvula and buccal mucosa. The disease mostly affects children. The aim of this case report is to discuss the oral symptoms of herpangina based on current literature for dental and health practitioners. In our case, a 15-month-old baby girl presented with high fever reaching 40.2 °C for 2 days with no seizures, cold and cough. Intra oral examination showed several well-defined whitish round lesions on the soft palate and one on the upper left lip. The patient was prescribed 3ml of methisoprinol 250 mg 3 times a day, 4 ml of paracetamol 125 mg every 6 hours, hyaluronic acid spray 3 times a day, and 1ml of vitamin C 40 mg taken once a day. The patient showed no sign of bacterial infection. Lesion disappeared after 7 days of treatment. Upon follow-up visit after 2 weeks, the lesion had completely disappeared. Dentists should be able to recognize and manage herpangina cases in infants
Characterization of β-tricalcium phosphate derived from green mussel shells (Molarity variation)
β-Tricalcium phosphate (β-TCP) is a widely used bioceramic material. In dentistry, it is commonly used as bone graft material. β-TCP is osteoconductive, bioresorbable, bioactive, and has biocompatibility properties. This study aims to evaluate the optimum molarity of CaO and H3PO4 to synthesize β-TCP from a natural source (Perna viridis linn). This is laboratory experimental research conducted by reacting calcium compounds from green mussel shells and phosphoric acid using the dissolution precipitation method with variations in molarity ratio. X-ray diffraction (XRD), scanning electron microscope (SEM), and fourier transform infrared (FTIR) were used to identify the characteristics of β-TCP synthesized from green mussel shells. The XRD chart pattern showed the formation of peaks identical to the β-TCP (Sigma-Aldrich). However, formation of whitlockite phase was also seen in the results. FTIR results showed that phosphate, hydroxyl, and carbonyl groups were shown on the graph and could be identified as β-TCP. SEM characterization showed that the sample consisted of small particles irregularly shaped to form like aggregates. β-TCP synthesized using molarity ratio of 0.6M CaO: 0.4M H3PO4 had characteristics resembling β-TCP (Sigma-Aldrich)