Majalah Kedokteran Gigi Indonesia
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Comparison of apical sealing ability between bioceramic and zinc oxide eugenol-based sealer during root canal treatment, in vitro
No full textObturation with a sealer material that provides an adequate apical sealing ability is required to prevent endodontic treatment failure due to microleakage. However, there are no sealers that meet all the physical and chemical properties to be able to hermetically seal the root canal system to date. Various sealer materials have been developed in recent years including the use of bioceramic materials which are claimed to have excellent biocompatibility to tissues. This study aimed to compare the apical sealing ability of bioceramic-based and zinc oxide eugenol (ZOE)-based sealer in root canal treatment. A total of 27 extracted mandibular premolars were decoronated to the standard root length of 14 mm. The root canals were prepared with a crown-down technique using manual instrument to file F3 (30/.09). The samples were then divided into three groups: obturation with bioceramic-based sealer (n=9); ZOE-based sealer (n=9); and control group (n=9). Microleakage was measured using a dye penetration method with 1% methylene blue and observed under stereomicroscope at x20 magnification. The mean of the maximum penetration length from the lowest to the highest was found in the bioceramic-based sealer group (0.825 mm), the ZOE-based sealer group (3.850 mm), and the control group (4.444 mm). One-way ANOVA test showed a significant difference in the maximum penetration length between the three groups (p<0.05). The post hoc LSD test showed a significant difference in the maximum penetration length between the bioceramic-based and ZOE-based sealer groups (p<0.001). Obturation with bioceramic-based sealer provides a better apical sealing ability than that with ZOE-based sealer
The Effectiveness of injectable platelet-rich fibrin and bone graft addition to open flap debridement for infrabony pocket therapy
No full textVarious biomaterials have been utilized as additional material to help tissue regeneration in the open flap debridement (OFD) procedure. Platelet-rich fibrin (PRF) is an autologous platelet concentrate that contains many growth factors (GFs) and generally used as an additional biomaterial in OFD. However, PRF has several weaknesses, such as its consistency, which is hard to mix with another biomaterial, and its abundant sediment of regenerative cells resulted from high-speed centrifugation. Injectable Platelet Rich Fibrin (I-PRF) is one of the PRF derivatives that contain more regenerative cells. Due to the low-speed centrifugation, I-PRF has a liquid form. Certain GFs were also found in I-PRF, such as PDGF, TGF-β1, VEGF, IGF, EGF dan COL-Ia. In this study, we measured probing depth (PD), relative attachment level (RAL), and bone height as the healing parameter, to see the effectiveness of I-PRF addition and bone graft in the OFD for infrabony pocket as the aim of this study. Twenty (20) periodontal pockets were chosen (PD= 5-7 mm) and divided into OFD+I-PRF+bone graft (bg) group (group I) and OFD+PRF+bg group (group II) equally. The clinical evaluation was measured at the baseline, day-30, and day-90. Results showed that PD (p= 0.022) and RAL (p= 0.008) significantly increased in group I (PD 2.80 ± 0.42; RAL 2.50 ± 0.52) compared to group II (PD 2.10 ± 0.73; RAL 2.00 ± 0.67); however, there was no significant difference in the bone height (p= 0.194). Taken together, our data revealed that I-PRF+bg induces soft tissue regeneration in infrabony pocket treatment
MIC and MBC of red fruit extract (Pandanus conoideus Lam) against periodontal pathogens bacteria
No full textThere are only few studies on the antibacterial activity of red fruit extract (Pandanus conoideus Lam) against oral pathogenic bacteria. Thus, this study aims to determine the effectiveness of red fruit extracts by looking at the Minimum inhibitory Concentrations (MIC) and Minimal Bactericidal Concentrations (MBC) against periodontal pathogenic bacteria. The subjects of this study were Streptococcus mutans (ATCC 25175), Fusobacterium nucleatum (ATCC 25586), and Porphyromonas gingivalis (ATCC 33277). The antibacterial effectiveness of red fruit extract was tested by the liquid dilution method (microdilution). The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Post Hoc Least Significance Different (LSD) test method. The red fruit extract effectively inhibited and eliminated test bacteria (p <0.05). Our study showed that the red fruit extracts at a concentration of 20% could inhibit the growth of Streptococcus mutans and Porphyromonas gingivalis, which was determined as the MIC strength of 80% as MBC of both bacteria tested. Furthermore, red fruit extract at the concentration of 10% showed an inhibitory effect on the growth of Fusobacterium nucleatum, which was determined as MIC of Fusobacterium nucleatum and the strength of 40% as MBC of Fusobacterium nucleatum. The red fruit extracts were significantly effective against the growth of Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis provide essential information for further in vivo clinical studies to determine the exact dosage and clinical effectiveness of periodontal disease
Nickel ion release of niti archwire in variations of immersion time and toothpaste
No full textNickel-titanium archwires in orthodontic wires have nickel ion releases. The use toothpaste and saliva immersion time may cause its releases. This study aims to prove the difference and interaction between immersion time and toothpaste variations towards the amount of nickel ion releases at nickel-titanium archwires. The study was an experimental research with a post-test only control group design. Twenty seven nickel-titanium archwires were divided into three groups, i.e., K group, which were immersed in artificial saliva. P1 was immersed in artificial saliva and 1.5 gram of toothpaste A, and P2 was immersed in artificial saliva, and 1.5 gram of toothpaste B. The samples were incubated (37 °C) for 1 and 1.5 months. Nickel ion released was analyzed using SSA. Two Way ANOVA and Post Hoc LSD test were the statistic test used. The results of this study were the K group which released 0.112 mg/l and 0.685 mg/l, P1 which released 0.093 mg/l and 0.670 mg/l, and P2 which released 0.099 mg/l and 0.657 mg/l. There were differences in toothpaste variations (p = 0.029), differences in immersion time (p = 0.000) and there was no interaction between immersion time and toothpaste variations (p = 0.505) on the amount of nickel ion release. There was a significant difference in K-P1 (p = 0.038), K-P2 (p = 0.013), P1-P2 (p = 0.049). There was a difference in immersion time and toothpaste variation, but there was no interaction between immersion time and toothpaste variation towards the amount of nickel ion releases
Protesa maksilofasial kerangka logam dengan hollow bulb pada kasus klas I aramany pasca hemimaxillectomy
No full textEffect of low-intensity pulsed ultrasound on mast cell degranulation and fibroblast expression on type 2 diabetes mellitus rats wound healing process
No full textImpaired wound healing is one of the Diabetes mellitus complications. Low-intensity Pulsed Ultrasound (LIPUS) therapy may accelerate the impaired wound healing. The use of LIPUS therapy in the early inflammatory phase can induce mast cell degranulation, and in the proliferative phase it can increase collagen synthesis by fibroblasts. The purpose of this study was to determine the effects of LIPUS therapy on mast cell degranulation and fibroblastexpression in the healing process of punch biopsy wound in rats with type 2 diabetes mellitus. Twenty-four Sprague dawley (n=24) were designed into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then divided into six groups: diabetes mellitus without LIPUS (DM3, DM7, DM14) and diabetes mellitus with LIPUS (DML3, DML7, DML14), 4 each, and punch biopsy wounds were made on the dorsal skin. The DML group received LIPUS therapy in the wound area (frequency 3 MHz, intensity 0.5 W/cm2, duty cycle 20%, duration 3 minutes every day for 3 days (DML3), 7 days (DML7), and 14 days (DML14). The wounded tissue area was stained with toluidine blue to observe mast cell degranulation and immunohistochemical type HSP-47 to observe fibroblast expression. Two-Way ANOVA and Post Hoc LSD tests were used to determine the differences in mast cell degranulation and fibroblast expression. The results showed that mast cell degranulation and fibroblast expression in the DML group were higher than in the DM group (table 1). Pearson test showed a correlation between mast cell degranulation and fibroblast expression (p=0.00; r= 0.839). LIPUS therapy increases mast cell degranulation and fibroblast expression in type 2 diabetes mellitus rat model. The higher the mast cell degranulation, the higher fibroblast expressions
Reference values for Down’s cephalometric analysis in Papuans
No full textThe asessment of cranio-facial structures forms a part of orthodontic diagnosis and treatment. One of the most frequently used cephalometric analysis is the Down’s analysis. It consists of 10 parameters of which 5 are skeletal and 5 are dental. Down has based his findings on 20 Caucasians, which have physical characteristic differences with Papuans. Indigenous Papuans are people of Melanesian descent consisting of indigenous tribes in Papua province and/or people who are accepted and recognized as Papuans by indigenous Papuans.The use of cephalometric values in different racial types, may result in the incorrect orthodontic diagnosis and treatment plan. According to those, the objectives of this study are to determine reference values for Down’s cephalometric analysis in papuans, to evaluate the differences exists between Papuan adult male and female, and also to compare the mean difference between persent study and established value of Down’s norms. A study was conducted on 16 lateral cephalograms of Papuans that have been selected based on inclusion criteria. The Down’s method was used for the cephalometric analysis. The data obtained was processed statistically to determine the mean, standart deviation, and minimum-maximum value, and presented in the form of frequency, table, or diagram. The result showed that the normal cephalometric values of papuans different from the normal values used in the Down’s analysis. The values obtained in this research is expected to be a reference in conducting Down’s cepahlometric analysis in Papuans for orthodontic treatment purposes
Periodontal status in patients with Alzheimer’s disease: a scoping review
No full textPeriodontitis is the most common condition of chronic periodontal infection and inflammation in the elderly population. Periodontal disease can cause local inflammation that contributes to higher risk of systemic inflammatory disease. Current research suggests a possible link between periodontal disease and neurodegenerative disease, such as Alzheimer’s disease. The aim of the study is to provide a profile of the periodontal tissue status in people with Alzheimer’s disease. This scoping review followed Joanna Briggs Institute (JBI) guideline, and the searching was conducted using PubMed, Cochrane, and EBSCOHost databases during February 6th – February 7th, 2021 with keywords of periodontal disease, periodontitis, periodontal inflammation, Alzheimer’s disease and dementia. It also included MeSH terms of “periodontal disease” and “Alzheimer’s disease” if available. Additionally, snowballing technique was used to include more articles. The identification and writing process for this article followed the PRISMA-ScR framework. There were 60 articles included in this study. This scoping review shows a profile of general characteristics including decreased oral hygiene and periodontal tissue status showed by high score of plaque and calculus, gingival inflammation, high percentage of Clinical Attachment Loss (CAL), Bleeding on Probing (BOP) along with deterioration of cognitive function. There was a decreasing level of periodontal health along with the decline in cognitive function experienced by AD participants. However, further research is needed to see the mechanism of this relationship
The effect of aerosol suction distance during brushing brackets of orthodontic patients on the presence of Streptococcus and Staphylococcus
No full textDentists are one of the professions most at risk of infection transmission because dental care potentially produces many infectious aerosols for bacterial transmission. Using personal protective equipment and aerosol suction that meets the standards for dentists is vital to prevent cross-infection in patients in practice. This study aims to determine the effect of aerosol suction distance on the orthodontic patient bracket brushing process on total plate number and the presence of Streptococcus and Staphylococcus on the dentist’s face shield. The method used is the swab method on the face shield used by dentists after brushing orthodontic patient brackets with variations in the distance of aerosol suction to the oral cavity of 10 cm, 15 cm, and 20 cm. After 2 x 24 hours of incubation, the total plate count was calculated, and Streptococcus and Staphylococcus were identified by analyzing the characteristics of the growing colonies and executing the catalase test. The results showed that the lowest total plate number found on a face shield with aerosol suction distance of 10 cm from the oral cavity, 1.23 ± 0.01 CFU/cm2. The one-way ANOVA test obtained a significance value of p < 0.05, concluding that the variation of the aerosol suction distance affects the total face shield plate number. The presence of bacteria on the face shield was negative for Streptococcus and positive for Staphylococcus
