Majalah Kedokteran Gigi Indonesia
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Prevalence and identification of oral candida species in patients with type 2 diabetes in Yogyakarta
No full textPatients with diabetes are prone to recurring and even resistant Candidiasis, making treatment challenging. Many hypotheses proposed related to susceptibility of diabetic patients to Candida. The prevalence and species of Candida in a particular diabetic community might be different compared to other diabetic community. This study aimed to determine the prevalence of Candida colony and its species in the oral cavity of diabetic patients included in the CDM (Chronic Disease Management) program in Yogyakarta. One hundred patients with type 2 diabetes (n= 100) were recruited as the subjects of this study. The subjects were classified into controlled and uncontrolled Diabetes mellitus (DM). Samples of oral rinse solution were collected to determine the species of Candida and number of Candida colonies using CHROMagar Candida medium. There were 47 and 53 of subjects with controlled and uncontrolled DM, respectively. The mean number of Candida colony in the subjects with controlled diabetes (1003.13) was higher than that in the subjects with uncontrolled diabetes (478.43). The Candida colony most commonly identified in the subjects with controlled and uncontrolled diabetes were C. albicans and C. glabrata, respectively. Female patients had higher mean number of Candida colony (859.51) compared to male (299.21). The Candida colony most often identified in both genders was C. albicans. In addition, the subjects of this study consisted of 83 geriatric subjects and 17 non geriatric subjects, in which the mean number of Candida colony in the geriatric subjects (761.77) was higher than that in the non-geriatric subjects (545.71). The Candida colony most often identified in the geriatric subjects and non-geriatric subjects was C. albicans and C. glabrata, respectively. The Mann Whitney test demonstrated that there was a significant difference (p = 0.009) of the mean number of Candida colony between male and female. However, there was no significant difference of the mean number of Candida colony between ages (p = 0.060) and diabetic status (p=0.175). It can be concluded that the Candida species most commonly identified in all the subjects was Candida albicans with the mean colony number of 349.96, followed by C. glabrata (225.97), C. krusei (144.91), C. tropicalis (3.67), and other species (2.02)
Antimicrobial photodynamic therapy with erythrosine photosensitizer against immune response in chronic periodontitis model
No full textChronic periodontitis is a progressive inflammatory disease of the supporting tissues of the teeth caused by dental plaque bacteria with a clinical sign of periodontal pockets. A Gram-negative bacterium that can trigger this inflammatory disease is Porphyromonas gingivalis. Antimicrobial photodynamic therapy with blue LED light irradiation and photosensitizer erythrosine can reduce the survival rate of P. gingivalis. This study aimed to determine the effects of antimicrobial photodynamic therapy (APDT) exposure with blue LED light irradiation and PS erythrosine on the number of macrophages, lymphocytes, and gingival fibroblasts in gingival tissue of Sprague Dawley rats as chronic periodontitis models. This study used a posttest-only control group design to examine 27 Sprague Dawley rats which were divided into P group (healthy rats), N group (untreated chronic periodontitis rats), and PDT groups (chronic periodontitis model given 1 mg/ml PS erythrosine and irradiated with blue LED light for 60 seconds). Cell observation of histologic preparations of rat gingival tissue with hematoxylin-eosin (H&E) staining was carried out on the 1st, 3rd, and 5th days. Histological preparations of gingival tissue with H&E staining was carried out on the 1st, 3rd, and 5th days. Statistical analysis used a one-way ANOVA and the Kruskal-Wallis test, continued with LSD and the Mann-Whitney post-hoc tests. Results showed significant difference in the mean of macrophages in the PDT group compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). The mean lymphocyte in the PDT group was significantly different from the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05), and significantly lower than that in the healthy group (p < 0.05) but only on the 3rd and 5th days. The mean fibroblast in the PDT group was significantly different compared to the untreated chronic periodontitis group on the 1st, 3rd, and 5th days (p < 0.05). In conclusion, there were significant differences in the number of macrophages, lymphocytes, and fibroblasts in a chronic periodontitis rat model after treatment with APDT exposure with blue LED and erythrosine photosensitizer
Impregnation of various fiber tapes toward mechanical properties of dental fiber-reinforced composites
No full textSynthetic dental fiber tape for fiber reinforcement (FRC) restoration is relatively costly and its availability is still limited in Indonesia, so natural dental fibers have been used as an alternative material. The aim of this study was to assess the effect of impregnation of various fiber tape toward the flexural strength and hardness of FRC. The materials used were natural Bombyx mori silk fibers (Indonesia), dental polyethylene tape (Construct Kerr, USA), dental Quartz tape (Quartz Splint UD, France), dental E-glass tape (Everstick TM, GC, Japan), silane coupling agent (Ultradent, Jordan), and composite resin (Denfil-Flow, USA). Five groups of samples consisted of FRC with various fiber tapes were prepared: unidirectional-silk, braided-silk, quartz, polyethylene, and E-glass. The five groups of FRC were tested to determine the flexural strength and hardness. The results were analyzed by one-way ANOVA, followed by LSD test. The results showed that the highest flexural strength was in the quartz group (496.84 ± 109.14 MPa), while the lowest was in the braided-silk group (139.39 ± 4.30 MPa). The highest hardness property was in the unidirectional-silk group (141.29 ± 25.17 VHN), while the lowest was in the braided-silk group (139.39 ± 4.30 VHN). The ANOVA showed that various fiber tapes significantly influenced the flexural strength and hardness of FRC (p < 0.05). The LSD showed that the unidirectional-silk, braided-silk, and polyethylene groups demonstrated no significant difference. The LSD for hardness showed that the unidirectional-silk group had a significant difference with the other groups (p < 0.05). It can be concluded that various fiber tapes influenced the flexural strength and hardness of FRC. Natural silk fibers showed comparable flexural strength and hardness with the other fiber tapes
Complex aestheticss treatment of six maxillary anterior teeth with smile design and deep bite correction
No full textDental trauma mostly damages the maxillary anterior teeth, affecting the individual’s esthetics, functionality, and psychological well-being. Restorative dentistry treatment is required to obtain a natural appearance based on the smile design approach. The aim of this report is to characterize the procedure of complicated cosmetic restoration of the six maxillary anterior teeth, which results in straighter and more beautiful natural-looking smiles. A 32-year-old male patient presented with multiple crown fractures in the anterior maxillary teeth, resulting in an unaesthetics smile. The patient also had a deep bite. A comprehensive aesthetics dental treatment was carried out. The dental treatment plan had to be carefully executed by considering several factors such as dental convexities and contours, re‑establishment of function, and esthetics. Smile design should always be approached as a multi-factor decision-making, which enables the doctor to treat patients using a personalized and interdisciplinary approach
Effectiveness of Aspergillus sp. extract in denture adhesive on surface roughness of acrylic resin on Candida albicans biofilm formation
No full textThe denture adhesive increases retention on the denture base and affects oral microorganisms. Adding antifungals to denture adhesives can inhibit the Candida albicans biofilms formation and prevent denture stomatitis. The combination of denture adhesives and herbal medicines is an alternative to antifungals, which have few side effects because it is a plant. Moreover, one of them is the endophytic Aspergillus sp. extract containing chemical compounds that can inhibit the Candida albicans biofilms formation. This study aims to analyze the effectiveness of the endophytic Aspergillus sp. extract in denture adhesive materials for Candida albicans biofilm formation on acrylic resin surfaces. The research method is to extract the Aspergillus sp. extract antibiofilm test. Denture adhesive formulation was adjusted to the standard, and added Aspergillus sp. with concentrations of 3.125%, 6.25%, 12%, and 25%. The research sample used hot polymerized acrylic resin. The control group used X denture adhesive and added nystatin, each group suspended by Candida albicans for 24, 48, and 72 hours. Examination of biofilm formation activity on the surface of acrylic resin used SEM. The analysis used Two Way Anova. Aspergillus sp. extract in denture adhesive effectively prevents Candida albicans biofilm formation within 24 hour incubation time. In conclusion, extract of the endophytic Aspergillus sp. in denture adhesive can inhibit the formation of Candida albicans biofilm on the surface roughness of acrylic resin
A potential of Jasminum sambac (L.) Aiton leaf nano-extract as spray treatment of gingivitis-induced Sprague Dawley rats
No full textGingivitis is the second most common disease that occurs in the oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such as allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, and tannins compounds reported to have an influence on wound healing process. The aim of this research was to determine the effect of observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract on the number of neutrophils, macrophages, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rats. Sixty male Sprague Dawley rats aged 2.5-3 months with body weight of 200-250 g were anesthetized with ketamine HCl (10 mg/kg BW) and xylazine (2 mg/kg BW) then induced using silk ligature 3.0 on the interdental of the mandibular incisor. The rats were divided into five groups and got daily spray using distilled water (negative control), nano-extract at a concentration of 40%, 45%, 50%, and 0.12% chlorhexidine (positive control). The rats were euthanized on the 1st, 3rd, 5th, and 7th days post-treatment. The tissues were processed histologically with HE staining. The number of neutrophils, macrophages, and angiogenesis were counted using a microscope (400x) and OptiLab Viewer® (13x) at five fields of view. The result of the Two Way ANOVA test showed that there were significant effect of concentrations and observation time, and interaction of both observation time and concentrations on the number of neutrophils, macrophages, and angiogenesis (p < 0.05). It can be concluded that observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract affected the number of neutrophils, macrophage, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rat (p < 0.05)
In vitro study of the stretching time-associated force magnitude generated by NiTi and stainless-steel closed coil springs
No full textClosed coil spring is an additional tool in a fixed-orthodontic treatment. NiTi is an orthodontic alloy that has shape memory and elasticity features, while stainless steel has properties of malleability, low friction between wire-bracket, as well as easy soldering and welding. Various properties of closed coil springs have been widely studied, but research on the stretching time remains unclear. This study aimed to determine the effect and differences in the stretching time for 7 days, 14 days, 21 days, and 28 days on the force magnitude of NiTi and stainless-steel (SS) closed coil spring. A 7 x 5 x 1 cm acrylic blocks with 1 cm acrylic posts were employed, and 25 mm closed coil springs were attached to the acrylic posts using modified T-sticks. The springs were immersed in an artificial saliva with pH of 6.75 for 7, 14, 21, and 28 days in an incubator at 37 °C. The container was removed from the incubator and the force was measured using a universal testing machine. Kolmogorov-Smirnoff test was carried out to analyze parametricdata, while independent T-test was used to determine the difference between each group. There was a significant effect of stretching time on the force magnitude of the SS closed coil spring in all the groups (p 0.05) but there was a significant effect on day 28 (p < 0.05). A significant effect between the ratio of the SS and NiTi closed coil spring (p < 0.05) was confirmed. Longer stretching time indicated a force loss in the SS closed-coil spring, while the NiTi spring had a more stable force which implies a formidable treatment-time for orthodontic treatment
A histological evaluation of cellular response on bone regeneration scaffold
No full textBiocompatibility testing for a new implant material is necessary before clinical use. The materials for bone scaffolding must also go through biocompatibility tests both in vitro and in vivo. The purpose of this study was to compare different staining methods in histological preparations to assess the biocompatibility of the material to tissues. Twenty Wistar rats (n= 20), aged 4 months, males, weighing about 250-350 g were divided into 2 research groups, namely sub-cutaneous and bone implantation for 3, 14, and 30 days. The sample was a biodegradable material with gypsum and calcium carbonate as the basic ingredients. Histological stains were prepared in several ways using Hematoxylin Eosin (HE), Mallory, Toluidine Blue, Tartrate-resistant acid phosphatase polyclonal antibody (TRAP), and Chromogen 3,3 diaminobenzidine (DAB) after pre-performing a decalcification process with ethylenedinitril-o-tetra-acetic acid (EDTA). The observation was done using magnification ranging from 10X10 to 100X10 with a light microscope (Nikon eclipse E600) and OptiLab viewer. The results were displayed in the form of descriptions of images generated from different staining methods. Staining by the HE method is most often chosen because it is cheap and easy but the other staining methods such as Toluidine Blue, Mallory, and TRAP show better contrast. Tissue staining techniques with immunohistochemistry (DAB, VEGF) are used to evaluate cell and tissue responses by identifying specific proteins present
Association between childhood obesity, oral health behavior, and dental caries in Jetis, Yogyakarta
No full textThe number of school-age children with overweight and obesity is increasing in developing countries. This is a risk factor for prosperity improvement followed by lifestyle changes. People with obesity are reported to be more susceptible to caries than people who are not, and the average numbers of caries in permanent molars increase on higher BMI. Dental caries is an infectious disease as a result of demineralization in enamel and dentine which has a relationship with behavior. The purpose of this study was to determine the relationship between oral health behavior and dental caries among overweight and obese children in Jetis District, Yogyakarta. This research was an analytical observational study with a cross sectional design. One hundred and ninety-six overweight and obese elementary school children in Jetis District were chosen using multistage cluster random sampling. The independent variable was behavior and the dependent variable was dental caries. Behavior variables were measured using questionnaire based on a Likert scale with validity testing (correlation coefficient > 0.30) and reliability testing (Cronbach alpha = 0.819). The caries was measured using teledentisry during the COVID-19 pandemic. Data analysis used Spearman’s correlation analysis. Behavior of maintaining oral and dental health in overweight and obese children had a relationship with caries (p < 0.001 and r = -0.410). There is no difference between overweight and obese children in maintaining their oral health behavior. The better the oral health behavior of the overweight and obese children, the lower the incidence of caries
Degree of facial profile convexity using Subtelny’s analysis in patients aged 6 to 12 years
No full textSkeletal malocclusion is caused by a disproportion of the maxilla and mandible, which leads to an unharmonious facial profile. Straight, convex, and concave soft tissue profiles may serve as a guide to determine the underlying skeletal relations and location of the jaws from the anteroposterior plane. In this study, Subtelny’s cephalometric analysis of the skeletal and soft tissue profile is used to evaluate the facial profile convexity and is divided into three parts, which are the skeletal profile convexity, soft tissue profile convexity, and total soft tissue profile convexity. The purpose of this study is to determine the degree of facial profile convexity according to Subtelny’s analysis in patients aged 6 to 12 years at Faculty of Dentistry Dental Hospital of Trisakti University. This was a descriptive observational study using samples of cephalograms of 40 patients aged 6 to 12 years. Cephalometry tracing and identification of anatomical landmarks were done to acquire the facial profile convexity degrees. The anatomical landmarks used were nasion, A-point, pogonion, soft tissue nasion, subnasale, pronasale, and soft tissue pogonion. The results of this study found that the average degree of the skeletal profile convexity was 171.26°, the soft tissue profile convexity was 166.18°, and the total soft tissue profile convexity was 144.83°. The degrees of skeletal profile convexity, soft tissue profile convexity, and total soft tissue profile convexity could be influenced by age, gender, and differences in growth and changes in the skeletal and facial soft tissue structures