Majalah Kedokteran Gigi Indonesia
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Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells
No full textPolyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels
Non-Dental glass fiber impregnation on flexural strength of fiber reinforced composite
No full textThe availability of dental E-glass fibers for Fiber Reinforcement (FRC) restoration is limited in Indonesia with relatively high cost.Therefore, non-dental glass fibers have been used as an alternative material. The composition of non-dental glass fibers is almost the same with dental E-glass fibers. An important parameter responsible for the strength of FRC is the impregnation of the fibers with resin. Reinforcing fibers are difficult to impregnate with the resin systems of high viscosity. The aim of study is to assess the effect of non-dental glass fibers impregnation using bis-GMA and TEGDMA resin on the flexural strength of FRC. The materials used in the study were non-dental glass fibers (CMAX, China), resin bis-GMA (Sigma-Aldrich, USA) and TEGDMA (Sigma-Aldrich, USA). Three groups of samples consisted of FRC with non-impregnated fibers, FRC with impregnated fibers (bis-GMA:TEGDMA=4:1) and FRC with impregnated fibers (bis-GMA:TEGDMA=1:1). The three groups were tested to determine flexural strength according to ISO 10477. The samples were stored in aquadest at 37 °C for 24 hours prior to flexural strength test. The results were analyzed by one way ANOVA with LSD post hoc test. The results of study showed that the lowest mean of flexural strength (116.16 ± 15.87MPa) was FRC with non-impregnated fiber and the highest mean (151.32 ± 23.74 MPa) was FRC with impregnated fiber (bis-GMA:TEGDMA=1:1). The statistical analysis showed that fiber impregnation had a significant effect (p< 0.05). It can thenbe concluded that non-dental glass fiber impregnation using bis-GMA and TEGDMA resin could increase the flexural strength of FRC and FRC with the impregnated fibers (bis-GMA:TEGDMA=1:1) that had the highest mean of flexural strength
Evaluation of surface roughness of the bracket slot before and after burning
No full textSlot bracket base is one important element to enable sliding movement of the archwire. Bracket reconditioning by means of burning changes the surface roughness of slot bracket base. The heat above 450 °C shows microstructure changes of the slot surface. On this basis, the present study aims to evaluate surface roughness changes of the slot bracket base before and after reconditioning by means of burning. Sixteen premolar brackets standard edgewise were divided into two groups each consisting of eight brackets. These two groups were tested at three different points using Scanning Electron Microscopy (SEM), with 3000 times of magnification. Subsequentl,group A was burned for 5 seconds (approximately 450 °C) and group B was burned for 10 seconds(approximately 643 °C). After treatment,these two groups were tested for the second time using Scanning Electron Microscopy (SEM), with 3000 times magnification and the images were analyzed using a discrete scale quantitative classification. Afterwards, the data were analyzed using ANOVA (p≤0.05) and post hoc analysis. The result showed that surface roughness of the slot bracket base burned for 10 seconds experienced more significant change than that of bracket burned for 5 seconds and that of the control bracket(p≤0.05).Bracket reconditioning using burning either for 5 to 10 seconds change the surface roughness of the slot bracket base
Effect of advanced platelet-rich fibrin applications on periodontal regeneration in infrabony pocket treatment
No full textOne of the regenerative periodontal treatments for infrabony pocket is open flap debridement (OFD) with the addition of growth factor derived from platelet concentrate. Advanced platelet-rich fibrin (A-PRF) is a further development of plateletrich fibrin (PRF) with a lower centrifugation speed (1,500 rpm, 14 minutes). The purpose of this study was to examine the differences in periodontal tissue regeneration after the application of A-PRF and PRF in the treatment of infrabony pockets evaluated from probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The samples were taken from 20 infrabony pockets divided into 2 groups: 10 subjects were treated with OFD+A-PRF and OFD+PRF on the remaining subjects. Probing depth (PD) and relative attachment loss (RAL) measurement were performed on days 0, 30, and 90. Bone height measurements were performed using CBCT X-rays on days 0 and 90. The results showed that PD and RAL reduction in the group of OFD+A-PRF was significantly greater than that in the OFD+PRF group. Bone height reduction in both groups showed no difference. The conclusion obtained from this study is A-PRF application enhances periodontal tissue regeneration by generating greater probing depth and relative attachment loss reduction compared toPRF, as well as an increase in bone height similar to in the treatment of infrabony pockets
The effect of TiO2 coating and coffee immersion on discoloration of thermoplastic nylon denture base
No full textThe thermoplastic nylon denture base material is prone to discoloration because its amide bonds absorb water easily. Titanium dioxide (TiO2) nanoparticle have long been used as a denture base coating. Meanwhile, coffee contains chlorogenic and tanic acid, which can change the color of denture bases. The purpose of this study was to examine the effect TiO2 coating and duration of coffee immersion on discoloration of thermoplastic nylon denture base. Samples consisted of 24 thermoplastic nylon in square-shaped (30 x 30 x 2 mm), divided into 4 groups (n = 6). They were control (without TiO2 coating) and treatment (with TiO2 coating) groups, which then were immersed in coffee solution for 15 and 30 days. Discoloration test was conducted using spectrophotometer by measuring the delta absorbance of light before and after coffee immersion. The result showed that the lowest delta absorbance was in the 15-day treatment group (0.011 ± 0.005) and the highest was in the 30-day control group (0.077 ± 0.027). Two-way ANOVA test showed that TiO2 coating and coffee immersion had an effect on discoloration of thermoplastic nylon (p <0.05). Post hoc LSD test showed that there were significant differences between the control and treatment group at 15 and 30 days of coffee immersion (p <0.05). In conclusion, TiO2 as a thermoplastic nylon denture base coating can reduce discoloration by coffee immersion for 15 and 30 days. There were no differences between 15 and 30 days of coffee immersion on thermoplastic nylon’s discoloration in the control and treatment groups
Effect of A’yun’s predictor software on the behavior, saliva pH, and PHPM index
No full textHealth Research in 2013 showed that 24.8% of children with health problems and dental caries index reached 1.4. A’yun’s Predictor Software (APS) was a new tool to describe the interaction of various factors that play a role in the caries process. The program was developed to establish a better understanding of the multi-factorial aspects of dental caries in children,as well as a guide to improve behavior to reduce the risk of new caries. This study in children aged 10-12 years in SDN Baturan I,SDN Baturan II,SDN Mayangan and SD Muhammmadiyah Trini in Sleman,Yogyakarta.Sampling technique was simple random sampling. This type of research was quasi-experimental control group(pretest and posttest design with control group). The independent variable was oral health education with APS and the dependent variable was the behavior of dental health maintenance oral and mouth, salivary pH and PHPM (Personal Hygiene Performance-Modified)index.Results data were analyzed by t-test. Research shown that there were significant differences between the treatment group and control group on the behavior of the maintenance of oral health of children and PHPM index (p 0.05). The conclusions of this study was significant APS application on dental health maintenance behavior oral and mouth, salivary pH, and the index PHPM in school children
The relationship between salivary secretion and taste sensitivity level in the elderly
No full textThe increasing population of the elderly has an impact on health problems, including an increase of oral problems. One of the common oral problems is dry mouth that possibly disrupts gustatory function. The aim of this study was to investigate the relationship between salivary secretion and taste sensitivity level in the elderly. This study was a cross sectional study for the elderly population of Daerah Istimewa Yogyakarta/The Special Region of Yogyakarta (DIY) Province, Indonesia. This study consisted of interview, clinical examination, measurement of salivary secretion (sialometry) and taste sensitivity level using chemical gustometry method. Salivary secretion data were described in ml/ min. The measurement of taste sensitivity level was performed using 4 different substances: sweet (sucrose), salty (NaCl), sour (citric acid) and bitter (quinidine sulphate) with 4 different concentrations for each flavor. Four score was given if the respondent recognized the lowest concentration of the substance and 0 score was provided when all concentrations were failed to be tasted. Total score for test sensitivity level was scaled from 0 to 16. Salivary secretion data and taste sensitivity level data were analyzed by Spearman correlation test to determine the correlation between both variables. There were 103 subjects (70 women and 33 men) with the age mean of 65.61 years old, participating in this study. The mean of the sweet taste level was 2.81, 3.32 for salty taste, 3.69 for sour taste and 2.98 for bitter taste. Total taste sensitivity level mean was 12.80 out of 16. The measurements of unstimulated whole saliva showed a mean value of 0.170 ml/min with the majority of subjects having normal salivary flow. The Spearman correlation test results showed r= -0.078 with p >0.05 that indicated no correlation. We concluded that there was no relationship between salivary secretion and taste sensitivity level in the elderly
Effect of jackfruit seed fortification on high calcium milk on bone collagen density
No full textOsteoporosis is a bone disease characterized by reduced bone mass and damage to the microarchitecture of bone tissue. One of osteoporosis types is primary type-1 osteoporosis or postmenopausal osteoporosis due to estrogen deficiency. Calcium is an important mineral that plays a central role in the formation of bone strength structure. Jackfruit seeds (Artocarpus heterophyllus) contain isoflavones that can replace the role of estrogen in the body. Fortification of jackfruit seeds and high calcium milk can be a potential alternative for the treatment of postmenopausal osteoporosis. The purpose of this study was to determine the effect of drinking high calcium milk fortified with jackfruit seed extract (Artocarpus heterophyllus) on alveolar bone collagen fiber density of menopause induced rats. Twenty-five Sprague dawley rats were included in this study. Twenty Sprague dawley rats were ovariectomized, while 5 rats were left untreated (normal control group). Seven days after ovariectomy was performed, 5 rats were treated with fortification of jackfruit seed extract and high calcium milk, 5 rats were treated with jackfruit seed extract, 5 rats were treated with high calcium milk, and 5 rats were left untreated (ovariectomized control group). The treatments were done for 28 days. The rats were euthanized and the alveolar bone was taken and processed for a histological specimen. Alveolar bone collagen fibers were observed under a binocular microscope with 100 magnification. Kruskal-Wallis result showed there was a significant difference in the alveolar bone collagen fiber density among these groups (p0.05). In conclusion, consuming high calcium milk fortified with jackfruit seed extract increases the density of alveolar bone collagen fibers
Streamline characteristics using the computational fluid dynamic analysis in the flow of 18% EDTA irrigation solution to remove Ca(OH)2
No full textThe remaining calcium hydroxide (Ca(OH)2) medicaments in the root canal wall can block the penetration of filling medicaments to the dentine tubule and cause the failure in the root canal treatment. One of the ways in cleaning the root canal wall from the remains of (Ca(OH)2) is by using 18% Ethylene Diamine Tetra Acetic Acid (EDTA) irrigation solution. The cleanliness of the root canal can be examined using the Computational Fluid Dynamics (CFD) analysis. The aim of this research was to see the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with 18% EDTA irrigation by means of CFD analysis. This is a descriptive-explorative research. Having been validated with the experimental research using test specimen in the form of block resin made in accordance with the characteristics of root canal, the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with the 18% EDTA irrigation was analyzed using CFD method. The irrigation needle used was the side-vented type with the position of 3 mm from the tooth apical. The results of the research showed the conformity between the result of experimental research and CFD research. One of the results of this research was the characteristics of streamline of 18% EDTA in the root canal showing a unique behavior due to the characteristics of the form of side vent irrigation needle. The irrigation flow in the coronal area of the inlet (side vent irrigation needle) showed a low velocity causing the more fluid flowing out from the inlet went to apical rather to the outlet (root canal orifice). In conclusion, this research showed that 18% EDTA solution indicated the conformity of validation results between experimental research and CFD research in the frames of 5, 10, 15, and 20 secs observed from experimental research with the of frame 0,010, 0,099, 0,150 and 0,410 secs as observed from CFD research
Anti-inflammatory role of papaya seed extracts in inhibiting osteoclastogenesis of rats with Periodontitis
No full textSome previous studies mentioned that papaya seed extract has anti-inflammatory activity because it contains polyphenols including phenolic and flavonoid acids. Polyphenols of the papaya seed extracts are known to inhibit the severity of periodontitis. It can prevent the release of excessive osteoclastogenesis which may promote bone resorption aggravating periodontitis. Hence, this study aims to determine the role of papaya seed extract in reducing the number of osteoclasts in rats with periodontitis. Twenty-eight male Wistar rats were divided into 4 groups of induced periodontitis using lipopolisakarida (LPS) Porphyromonas gingivalis (P. Gingivalis) and followed by wire ligature in “8” placed around the mandible mandibular incisor. The first group was given standard food without any papaya extract, while each rat in three other groups with periodontitis (P2, P3, P4) was given with Papaya seed extracts of 200 mg/kgBW, 300 mg/kgBW, and 400 mg/kgBW. Seven rats in one group were not induced by periodontitis and were fed with standard food as a control group. On the 30th day, the rat was sacrificed, and its transverse pieces of alveolar bone around the mandibular incisor were stained with Hematoxylin-Eosin. The data analyzed by one way ANOVA indicated a significant result. Thus, it is conclusive that extract of papaya seed ethanol inhibited osteoclastogenesis in Wistar rats induced with periodontitis