Majalah Kedokteran Gigi Indonesia
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The effect of in vitro royal jelly provision on adhesion of Pseudomonas aeruginosa
No full textPseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium, which could aggressively infect immunocompromised patients and thus, cause high mortality rate. In addition, P. aeruginosa in oropharynx could be aspirated and cause ventilator associated pneumonia. Royal jelly is one of bee’s products that has been used for therapeutic needs including antibacteria. Adherence factor of P. aeruginosa were flagelum, pili and lectin. The aim of the study was to determine the effect of royal jelly to P. aeruginosa adhesion. Suspension of P. aeruginosa (ATCC® 27853) was incubated at 37 °C for 18 h. Treatment groups were exposed to royal jelly with several concentrations, 2%, 4%, 6%; while distilled water was being used as negative control. Bacterial adhesion test was determined using spectrophotometer λ = 600 nm to measure optical density values of adhered bacterial suspension in tubes. The result of one-way ANOVA showed significant differences (p<0.05) of optical density values among groups indicating that royal jelly affected the bacterial adhesion. LSD results showed significant difference of optical density values between 2%, 4%, and 6% royal jelly compared to distilled water. Six percent of royal jelly had the least optical density value compared to the other groups. In conclusion, royal jelly has the ability to decrease adhesion of P. aeruginosa. Six percent of royal jelly has better ability to decrease adhesion of P. aeruginosa than other concentrations
Differences in results of infrabony pocket treatment with addition of platelet rich fibrin and platelet rich plasma gel in DFDBA bone graft
No full textTreatment of infrabony pocket makes use of bone graft material demineralized freeze dried bone allograft (DFDBA) from different individuals which has undergone demineralization process and it is osteoinductive. Enhancement of growth factor was done by adding platelet rich fibrin (PRF) and platelet rich plasma (PRP). PRP is activated with an addition of calcium chloride CaCl2) to form gel. The method used to apply the bone grafting material is open flap debridement OFD). This research aimed to reveal the differences in the results of infrabony pocket treatment using PRF and PRP gel with an addition of DFDBA. The sample was taken from 20 infrabony pocket points divided into 2 groups, 10 infrabony pocket were treated with OFD+DFDBA+PRF and the other were treated with OFD+DFDBA+PRP gel. Pocket depth (PD) was measured on the baseline and the first and third month after treatment. Alveolar bone height was measured using cone beam computed tomography (CBCT) radiograph on the baseline to the third month after treatment. The results of this research showed that there was difference in the results of infrabony pocket treatment using PRF and PRP gel with an addition of DFDBA which could be observed from a reduction in PD from the baseline, month 1 and month 3 as well as reduction in alveolar bone height from the baseline to month 3. This research concluded that infrabony pocket treatment PRF application yields better results than PRP gel application in terms of PD and alveolar bone height reduction
Apical sealing ability of chitosan nanoparticles in epoxy-resin-based endodontic sealer
No full textA good sealing ability of apical part of root canal system is needed to prevent microleakage thus resulting long-lasting successful treatment. Chitosan nanoparticles as nanofillers could be added to epoxy resin sealer to increase adaptation to dentinal wall. The purpose of this study was to evaluate the apical sealing ability of chitosan nanoparticles addition to epoxy-resin-based sealer. Thirty mandibular premolars were used in this study. The root canals were prepared using rotary files up to #30/0.09. The canal was irrigated with 2.5% NaOCl and 17% EDTA, then was rinsed with sterile water. All teeth were randomly divided into 3 groups (n=10). Group I was obturated with gutta-percha, Group II was obturated with gutta-percha and epoxy resin sealer, group III was obturated with gutta-percha and epoxy resin sealer that was added with chitosan nanoparticles. All specimens were stored in an incubator for 7 days at 37 °C. After that, specimens were tested with centrifuging dye penetration method with methylene blue 2% solution. The specimens were longitudinally sectioned, observed under stereomicroscope (8x magnification) and measured in millimeters (mm). The data were analyzed using one-way Anova. The results of one-way Anova showed that dye penetration in apical part of the root canal in all groups was statistically significant difference. The addition of chitosan nanoparticles to epoxy resin sealer increases the apical sealing ability of root canal obturation material
The correlation between serum zinc and vitamin D/25(OH)D in women with recurrent aphthous stomatitis
No full textRecurrent aphthous stomatitis (RAS) is an oral mucosal disease which was more prevalent in women than men. Study has shown that zinc deficiency associated with the occurance of RAS. Other study also have shown that vitamin D deficiency contributed in RAS. However, there is no study yet that reveal the association between serum zinc and vitamin D/25 (OH)D in women with RAS. To investigate the association between serum zinc and vitamin D/25(OH)D in women with RAS. Thirty two women patients with RAS who meet the inclusion criteria participated in this study. The inclusions criteria was non pregnant women who have been diagnosed RAS and did not have other oral diseases. The exclusions criteria were those who have systemic diseases, taking medications including multivitamin, and have bad habits (i.e. smoking). Data concerning characteristic of subjects, severity of RAS, serum vitamin D/25(OH)D, and zinc were collected and presented descriptively. The correlation between serum zinc and Vitamin D/25(OH)D was analyzed using Pearson correlation test with 95% confidence interval. This study has been approved by Medical and Health Ethics Committe, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta. All subjects of this study have low mean value of serum vitamin D/25(OH)D (11.08 ± 3.11 ng/mL) and categorized with vitamin D deficiency. The subjects also have low mean value of serum zinc (54.78 ± 9.19 μg/dL) and seventy percent subjects have mean value of serum zinc below normal. The result of Pearson correlation test showed that there was a significant positive correlation between mean serum zinc and Vitamin D/25(OH)D (r= 0.351, p<0.05) in women with RAS. Serum zinc associated with vitamin D/25(OH)D and play role in pathogenesis of RAS
The transversal strength comparison between polyethylene and glass fiber as an acrylic resin denture plate repair material
No full textAcrylic resin is the most commonly used denture base material. However, it has a shortage of being easily broken. One way to resolve this problem is by adding polyethylene (PE) or glass fibers. The purpose of this research is to compare the transversal strength of PE and glass fibers from denture plate acrylic resin repair material. The experiment involved 32 plates of heat cure acrylic with the dimensions of 65 mm x 10 mm x 2.5 mm. The speciments were prepared to create a 3 mm gap and 45° bevel. Subjects were divided into 2 groups, each group containing 16 plates. Group I was reinforced with PE fiber and Group II was reinforced with glass fiber. All plates were soaked in distillation water for one day at 37 °C. Plates were tested for transverse strength with universal testing machine and all data were analyzed with independent t-tes at 95% confidence level. Macro photo analysis was used to observed the bond failure on fiber and resin. The mean of transverse strength (MPa) denture plate acrylic resin repair material reinforced with PE fiber was (67.38 ± 4.31) MPa, while glass fiber was (93.61 ± 6.14) MPa. Independent t-tes showed that type of fiber had a significant effect (p<0.05). Thus, it is possible to conclude that addition of glass fibers in denture plate acrylic resin repair material increased the transverse strength and made it stronger than those added with PE fibers
The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats
No full textIndonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was 0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats
Differences in effectiveness of membrane fixation using periosteal vertical mattress and simple sling suture techniques in infrabony pocket treatment
No full textGuide tissue regeneration (GTR) is the treatment of infrabony pockets for soft and hard tissue regeneration. Membrane is used as a barrier and prevents apical migration of the cells in epithelial tissues. Membrane fixation is one of the procedures in GTR treatment because resorbed membrane is less stable. Simple sling suture technique for membrane fixation has 1 anchorage, located in coronal flap, while periosteal vertical mattress suture technique has 2 anchorages in periosteum, making it more stable. This study aimed to study the differences in the effectiveness of membrane fixation using periosteal vertical mattress suture and simple sling suture techniques in terms of probing depth, relative attachment loss, and alveolar bone height in the treatment of infrabony pockets. The samples were divided into 2 groups. The first group was open flap debridement (OFD) with demineralized freeze dried bone allograft (DFDBA) application and membrane fixation with simple sling suture, while the second group was OFD with DFDBA application and membrane fixation with periosteal vertical mattress suture, observed on day 0, 30th day, and 90th day. The results of the study showed significant differences in the probing depth and relative attachment loss (except from the 30th day to the 90th day), and there were no significant differences in the alveolar bone height from the baseline to the 90th day, between the group of membrane fixation using simple sling suture and that of periosteal vertical mattress suture techniques on flap surgery. This study concluded that membrane fixation in the treatment of infrabony pocket with periosteal vertical mattress suture technique is more effective in reducing the probing depth and relative attachment loss, but there is no difference in increasing the alveolar bone height when compared to simple sling suture technique
The effect of bitter gourd (Momordica charantia) ethanolic extract on inflammatory infiltrates and NF-κB activation in periodontitis
No full textPeriodontitis is a periodontal disease involving the gingiva, periodontal ligament, cementum, and alveolar bone due to an inflammatory process. Virulence factors of periodontopathogens and inflammatory responses in periodontitis can stimulate nuclear factor-kappa B (NF-κB) activity. Charantoside c and Momordicosides g in bitter gourd prevent NF-κB activation stimulated by TNF-α in HepG2 cells. This study aims to determine the effect of bitter gourd fruit (Momordica charantia) ethanolic extract as an anti-inflammatory substance on the level of inflammatory infiltrates and the number of cells that experience NF-κB activation in the periodontitis model. Eighty male Wistar rats were divided into 5 groups. The mandibular incisors were ligated for 14 days to induce periodontitis. Each group was given Momordica charantia extract of 500 mg/kg BW, 250 mg/kg BW, and 100 mg/kg BW; ibuprofen 100 mg/kg BW; and aquades orally using oral gavage on the day 14. Rat necropsy was carried out on day 1, 3, 5, and 7 after giving the substances. Taking out the lower jaw was done to make tissue preparations followed by staining them with hematoxylin eosin (HE). Immunohistochemicalanalysis was performed to observe cells that were positive for NF-κB activation. The results showed a decrease in the density of inflammatory infiltrates in all groups, except for those given aquades. The number of inflammatory cells ofneutrophils, macrophages, and lymphocytes that experienced NF-κB activation showed the most effective decrease in the group of Momordica charantia 500 mg/kg BW, on the 7th day. The conclusion of this study is that ethanolic extractof Momordica charantia has an anti-inflammatory effect and prevents the activation of NF-kB in rat gingival induced by periodontitis. The highest effect was found at a dose of 500 mg/kg BW on day 7 after giving of extracts
Effect of glass fiber non-dental’s length and position on the flexural strength of FRC
No full textGlass fiber non-dental has a similar composition to E-glass fiber dental that commonly used as fiber reinforced composite (FRC) materials in resin bonded prosthesis. Fiber effectiveness can be determined by the length and the position. The aim of this study was to examine the effect of glass fiber non dental’s length and position on the flexural strength of FRC in resin bonded prosthesis. This study has been done used 36 FRC samples with beam shaped (15 mm x 2 mm x 2 mm). Fiber reinforced composite samples were consisted of 9 groups (a combination between length: 4 mm, 6 mm, and 12 mm and position: compression, neutral, tension zone). The flexural strength was tested by universal testing machine and statistically analyzed using two-way ANOVA (p<0.05). The result showed that the lowest (compression, 4 mm) and the highest (tension, 12 mm) flexural strength were 104.30 ± 13.90 MPa and 166.18 ± 8.59 MPa. The two-way ANOVA test showed that variation of position, length, and interaction between placement-length had a significant effect on the flexural strength (p<0.05). The conclusion of this study was fiber position on compression zone with 4 mm length had the lowest flexural strength. In addition, fiber position on tension zone with 12 mm length had the highest flexural strength.
Differences between male and female mandibular length growth according to panoramic radiograph
No full textEvaluation of the mandible was used for age and sex determination in the forensic field and it established the treatment planning in dentistry. The present study aims to determine mandibular length growth on male and female group aged 9-25 years old using panoramic radiograph. The research was conducted using analytical cross-sectional design method. The subject of this research was 412 panoramic radioraphs of patients aged 9-25 years old. It involved a totalsamples of 207 males and 205 females. This research was conducted from May to October 2017 in RSGM Padjadjaran University Bandung to meassure the length and height of mandibula from the point in condyleus to mentone. The MannWhitney test results showed a significant difference in mandibular length between men and women and no significant difference between the right andleft mandibular length in men and women. There is a difference between male and female mandibular length growth, in which the increase of growth of mandibular length in female is earlier than that of male. Male mandibular length is greater than female