Majalah Kedokteran Gigi Indonesia
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Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity
No full textAcrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity testswere done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials
Potency of stored DNA derived from buccal mucosal epithelial cells for genotype analysis of interleukin 4 -590 C/T
No full textThe genotype variation of the interleukin 4 (IL-4) gene on spot -590 C/T is known to correlate with IL-4 protein expression in periodontitis, but the results found in the population are inconsistent. Sequencial genotype analysis of multiple genes often used stored DNA samples obtained from buccal mucosal epithelial cells. This study aims to determine the use of stored DNA samples obtained from buccal mucosal epithelial cells for genotype analysis specifically on -590 C/T IL-4 genes. This is a descriptive analytical study. The samples were 58 DNA derived from the buccal mucosal epithelial cells taken by sterile cotton swab isolated with PrestoTM kit (GeneAid). The DNA has been stored for 2 years at -20 °C. The genotype analysis was conducted using PCR RFLP technique with BSmFI enzyme and the data were presented descriptively. The results show that 51.72% of the stored DNA is still in good condition and 37.9% can be used for genotype analysis. It was concluded that DNA derived from buccal mucosal epithelial cells stored at -20 °C for 2 years can still be used for genotype analysis, but the quality of DNA is affected by storage time.
Oral health knowledge and practices of antenatal mothers in Hospital Universiti Sains Malaysia
No full textMothers play a very significant role in influencing the oral health status of the family. Many studies have highlighted the importance of good oral health during pregnancy. This study aimed to assess knowledge, self-perceived oral health status, and practices of antenatal mothers in Hospital Universiti Sains Malaysia, Kelantan, Malaysia. A total of 76 antenatal mothers visiting Obstetrics & Gynaecology specialist clinic at Hospital USM were involved in this cross-sectional survey study. The questionnaires contained items related to oral health knowledge, self-perceived dental problems, barriers to seeking dental care, oral hygiene habits, perceptions of oral health, and access to dental care. Majority (98.7%) agreed that their oral health was important as part of general health. About 58% of them had answered correctly regarding oral health knowledge. Most respondents had self-perceptions of having dental problems during pregnancy, including cavitated teeth (34.4%) and sensitive teeth (20.5%). Regarding practice, only 35.3% visited a dentist in the last six months. Time constraints (52.4%) and safety concerns regarding dental treatment (26.2%) were the main barriers to seek dental care. Many (90%) brushed their teeth at least twice daily with adult fluoridated toothpaste, while some used mouthwash daily (36.8%) and flossed their teeth (11.8%). Antenatal mothers had an average level of knowledge regarding oral health, experienced dental problems during pregnancy and lacked awareness of a regular dental visit. Hence, there is a need for more vigorous oral health promotion, which also include antenatal care providers, to improve oral health awareness among antenatal mothers
Dealing with the high-risk potential of COVID-19 cross-infection in dental practice
No full textThe World Health Organization reported that the SARS-CoV-2 virus has infected more than 5 million people around the world. Dental care providers and health care professionals need to be aware of the high-risk potential of crossinfection since the routes of virus transmission commonly happen through droplets and aerosols. This review aimed at collecting essential knowledge about the COVID-19 needed by dental practitioners. The review focused on the oral involvement in COVID-19, the role of oral transmission as the high-risk potential of cross-infection and recommended strategies to minimize the risk of cross-infection in dental practice. We searched all the published clinical features from PubMed, Google Scholar, Scopus and hand searched library online databases, from January 2015 until May 2020. Keywords used were “COVID-19”, “Dentistry”, “Dental protection”, “Cross-contamination”, “Aerosol and non aerosol”, and ”Povidone-iodine” with their combinations. We identified 52 articles to review after the initial selection with inclusion and exclusion criteria. Results showed use of topical applications of povidine-iodine and viricidal mouthwash could significantly reduce the high-risk of cross-infection from dentistry patients who are asymptomatic with COVID-19 infection. Further safeguards include suspending all non-emergency procedures temporarily and closely screening patients for symptoms which may be suspected to be COVID-19 infection
Effect of salat (prayer) activity on salivary status and cortisol level
No full textThe majority of Indonesian people are Muslim who perform the obligatory prayers five times each day known as salat. The series of activities in salat begins with wudu with a gargle movement that increases salivary secretion through mechanical stimulation. The movement of ruku and sujood when salat causes oxygen-rich blood to flow to the brain is well suited. When a person feels calmer and more psychically relaxed after performing salat and wudu, the parasympathetic nerves increase so normal cortisol levels can secrete more saliva which is aqueous with normal acidity (pH). Oral health will affect the overall health of the body. This study aimed to determine the effect of salat and wudu on the salivary flow rate, pH, and cortisol level as an indicator of oral health. The design of this study was the static group pretest and posttest design. The study was conducted by comparing the value of salivary flow rate, pH, and cortisol levels in 20 subjects consisting of 10 subjects as the treatment group and 10 subjects as the control. Saliva was collected without stimulation before and after salat activity for five minutes, followed by measurement of salivary flow rate and pH level. Salivary cortisol level was measured using Cortisol Assay Elisa kit with λ 540 nm. The results showed an increase in salivary pH, flow rate and cortisol level after the salat activity but there was no significant difference by Kruskal-Wallis test but there was a strong correlation using Kendall’s tau_b test. In conclusion, salat activity may have correlation with an increase in salivary pH, flow rate and cortisol level
Orbital cellulitis as a complication of odontogenic infection
No full textOrbital cellulitis is uncommon sequelae of the spread of odontogenic infection to adjacent maxillary sinuses or to distant sites such as the orbits. Once it happened, the spread of such infection can be of serious complication such as complete blindness or even more serious and life threatening situations as cavernous sinus thrombosis, intra-cranial abscess, or even death. This paper aimed to expose the guideline treatment of orbital cellulitis as a complication of odontogenic infection. It reported one case of orbital cellulitis in the emergency unit of Hasan Sadikin hospital Bandung, with complaints of pain, swelling at cheek and periorbital region, and the history of toothache. The treatment include incision drainage, extraction of tooth, and parenteral administration of antibiotic and analgesic. Odontogenic infections are derived from dental infection and can potentially spread rapidly to be ascending infection like orbital cellulitis. Odontogenic infection and orbital cellulitis should be adequately treated with incision drainage, extraction of tooth and parenteral administration of antibiotic, steroid and analgesic. Without immediate treatment, odontogenic infection can lead to ascending infection. Orbital cellulitis due to spreading of odontogenic infection is a rare case. In this case report, the patient had a significantimprovement due to immediate and proper treatment
Effect of addition titanium dioxide nanoparticles as acrylic resin denture base filler on cytotoxicity
No full textDenture base material should have a good level of biocompatibility. Acrylic resin is frequently used as a denture base material, however it has a disadvantage of producing residual monomer. Residual monomer is known to have a cytotoxicity effect. Titanium dioxide (TiO2) nanoparticles are used as fillers due to their biocompatibility and ability to enhance the mechanical properties of acrylic resin. The addition of the material to acrylic resin could affect the amount of residual monomer. The aim of this study was to examine the effect of the addition of TiO2 nanoparticles as acrylic resin denture base filler on the cytotoxicity in fibroblast cells. The samples consisted of 24 heat cured acrylic resins in disc shape (5 mm in diameter and 2 mm in thickness), divided into 4 groups (n = 6): three groups given treatment with0.5%, 1%, 2% TiO2, respectively and one control group. Cell viability was measured with MTT assay. The results were tested with one way ANOVA with 95% confidence level followed by LSD post hoc test. The results showed that the highest percentage of cell viability was found in the treatment group of 0.5% TiO2 with value of 91.83 ± 1.75%, while the lowest value was seen in the treatment group of 2% TiO2 with value of 79.38 ± 3.34%. Significant differences were shown between the treatment groups of 0.5% and 2% TiO2, as well as between the control and treatment group with 2% TiO2. The conclusions of this research are the addition of TiO2 nanoparticles as acrylic resin denture base filler has an effect on cytotoxicity; the addition of 0.5% TiO2 nanoparticles filler results in lower cytotoxicity on fibroblast cells compared to the addition of 1% and 2% TiO2.
Associations between influence of infant feeding patterns on the deciduous second molar relationship in preschool-aged children
No full textExclusive breastfeeding during the first six months of life is extremely important for the development of the dentocraniofacial structure, occlusion, breathing and swallowing during childhood. The prevalence severity rate of malocclusion in children who were breastfed is lower than those who were bottle-fed. The aim of this study is to evaluate the associations of infant feeding patterns on the deciduous second molar relationship in preschool aged children (3-5 years old children) of selected preschools in and around Medan Selayang. This study used analytic cross-sectional study design as the research method. The sampling was carried out by purposive sampling technique. This study involved 130 children aged 3-5 years as the sample, who consisted of two groups, the exclusively breastfed group and the bottle-fed group with the inclusion criteria of the children having a good oral and dental health. The result is statistically analyzed using Chi Square with a significance level of p<0.05. There was a significant difference between children who were breastfed and those who were bottle-fed. Children who were breastfed mostly had mesial step (55, 4%), followed by distal step(27.7%) and flush (16.9%), while those children who were bottle-fed mostly had distal step (46.2%), flush (33.8%), and mesial steps (20%). Based on Chi Square test, it is shown that there is a significant relationship between infant feeding patterns to primary molar relationships (p<0.001)
Quality of dental care services and satisfaction of patients visiting dental treatment center of Kahuripan public health center, Tawang sub district, Tasikmalaya city
No full textPatients, especially those who use health insurance have felt that the quality of primary care services tends to be less satisfying in terms of both public services and dental care services. Based on the results of the annual report at the Kahuripan Health Center, the number of patient visits to BPG decreased from 2016 to 2017 by 802 people. This study aimed to determine the quality of dental care services and the satisfaction of patients visiting BPG Kahuripan Health Center. This case study used a qualitative method and an explanatory research design. The sample in this study consisted of 40 patients as the respondents responding to the questionnaires about service quality and patient satisfaction, and 12 people were interviewed. The results showed that all dimensions of the quality of dental care services at the Kahuripan Health Center were categorized as very good i.e. real dimensions (82.71%), reliability (83.21%), responsiveness (87.12%), assurance (84.93%), and empathy (83.54%). As for patient satisfaction, three stages of patient satisfaction were categorized as very satisfied, namely the anamnesis and diagnosis stage (83.02%), action (83%), and completion of action (82.62%). The stages categorized as satisfying were the registration and waiting room stage (78.87%), and the stage of preparation of treatment (72.50%). The result of interview showed that the average respondents stated that the quality of dental care services was very good. All the five dimensions of the quality of dental care services were categorized as very good. Patients who visited BPG expressed satisfaction with the five stages, namely they were very satisfied with the three stages and satisfied with the other two stages
Effect of freeze-drying process of collagen-activated platelet-rich plasma on transforming growth factor-β1 level
No full textPeriodontal tissue damage requires regenerative material to repair the damage. Platelet-rich plasma (PRP) is known as a regenerative material from blood which contains high level of growth factor that plays a role in wound healing and tissue remodeling. However PRP has a weakness, i.e. it is too watery so it is easily dissolved in the oral cavity, and should be used immediately after preparation. Therefore PRP storage method is needed to increase the benefits of PRP. The addition of collagen to PRP serves as a scaffold as well as an activator that stimulates the release of growth factors. One method of storing PRP is by freeze-drying process. The purpose of this study was to analyze the effect of freeze-drying process of collagen-activated PRP (PRP+C) on transforming growth factor-β1 (TGF-β1) levels. Transforming growth factor-β1 is a cytokine content in PRP, that plays a role in bone remodeling and is an important stimulator for osteoblast formation, causing chemotaxis, osteoblast proliferation and differentiation. In this study, PRP was produced from peripheral blood probandus. Platelet-rich plasma was then activated with collagen (PRP+C), and divided into two groups: freeze-dried PRP collagen (FD PRP+C); and non freeze-dried PRP+collagen (PRP+C). Transforming growth factor-β1 levels were measured using the ELISA method, followed by independent t-test. The TGF-B1 level of FD PRP+C group was significantly higher than PRP+C group (p<0.05). From this study it can be concluded that freeze-dried collagen-activated PRP has an effect to increase TGF-β1 level