Majalah Kedokteran Gigi Indonesia
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The effect of acellular dermal matrix therapy on biphasic calsium sulfate bone graft
No full textInfrabony pocket therapy is needed to eliminate pocket wall, creating easy to clean conditions for new attachment, and bone regeneration. Biphasic calcium sulfate (BCS) bone grafts combined with collagen membranes are known to regenerate bone tissue and have good osteoconductive effects. The addition of collagen membranes promotes migration and proliferation of fibroblast cells, osteoblasts, and homeostasis. However, the collagen membrane is a rapidbioresynthesis and may cause disease transmission from animal. Acellular dermal matrix (ADMA) membrane contains a bioactive matrix that has the ability to support normal revascularization, cell repopulation, and tissue remodeling. Combination of BCS with ADMA membrane are proven to induce bone and tissue regeneration. The objective of this study is to determine the therapeutic effect of BSC and ADMA combination to eradicate pocket, gingival recession, bone recession and attachment loss. The samples were taken from 20 infrabony pocket sites divided into 2 groups. The first was treated by combination of BCS and ADMA, while the second group was treated by the combination of BCS and collagen membrane. After 1 and 3 months of flap surgery, the result was observed by probing depth(PD), relative attachment loss (RAL), gingival recession, alveolar bone height and radiological examination. The result showed the decrease of PD, RAL, gingival recession, and alveolar bone height in both two groups. However, there wereno significant differences between those two groups. In conclusion, the combination of BCS and ADMA or BCS and collagen decreased the PD, RAL, gingival recession and alveolar bone height
Effects of contact time of natural cu-zeolite on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa
No full textInfection of a surgical wound due to bacteria is a major problem for surgical patients. Cu-zeolite is a material that can suppress bacterial growth with reversible cation characteristics and adsorption to be developed into non-toxic disinfectants for humans. Packaging uses filter paper to keep disinfectant solutions or instruments that will be sterilized clean. This study aimed to observe the effects of contact time of natural Cu-zeolite on the growth of Streptococcus pyogenes (S. pyogenes) and Pseudomonas aeruginosa (P. aeruginosa) bacteria. An experimental research was simple randomized design. Cu-zeolite 10 grams were packaged in Whatman no 42 paper bags measuring 5 x 5 cm2, contacted for 15, 30 and 45 minutes in 99.5 ml of distilled water exposed to 0.5 x 108 CFU / ml of S. pyogenes and P. aeruginosa. Subsequently, bacterial growth was calculated using total plate count method. The average growth of S.pyogenes for 15, 30 and 45 minutes (1840 ± 571.236 CFU; 29 ± 16.33 CFU and 0 CFU) while P. aeruginosa was (2776 ± 725.277 CFU; 55 ± 23.214 CFU and 0 CFU) respectively. Based on the independent t-test on Cu-zeolite, the bacterial growth in the 15th and 30th minute contact between S. pyogenes and P. aeruginosa was significantly different (pth and 30th minute contact
Histopathological characteristics of dental socket healing on collagen density following use of pangas catfish (Pangasius djambal) gelatin
No full textTooth extraction is a common treatment in dental practice. However, complications such as bleeding or dry socket may occur during healing process. Pangas catfish (Pangasius djambal) gelatin contains glutamine which could increase the proliferation of fibroblast, accordingly collagen synthesis increases. The study aimed to demonstrate collagen density after the application of pangas catfish gelatin on post-tooth extraction wound of albino rats (Rattus norvegicus). This wasan experimental study with randomized posttest-only control group design which involved male Wistar strain albino rats. The rats were selected using a random sampling method and divided into four groups, namely day-3 control group (n=7),day-7 control group (n=7), day-3 treatment group (n=7) and day-7 treatment group (n=7). Pangas catfish gelatin was administered into the socket once after extraction. The variable measured in the study was collagen density, determinedby collagen scoring of hematoxylin eosin–stained histopathological preparations. Collagen formation were determined according to the assessment criteria of collagen distribution and density. Data analysis showed there was a significantdifference of collagen synthesis between the control and treatment groups according to Kruskal-Wallis test of which the result of control group day-3 was 1.13 ± 0.34, control group day-7 was 1.76 ± 0.67, treatment group day-3 was 1.66 ±0.47 and treatment group day-7 was 2.93 ± 0.25. In addition, the day-7 treatment group showed the highest collagen scoring values as compared to the control groups according to the Mann-Whitney test (p<0.05). In conclusion, pangascatfish gelatin increased collagen density during dental socket healing process after tooth extraction in albino rats
Quality of life patients after treatment of mandibular fractures with ORIF in oral surgery departement of Dr. Sardjito general hospital
No full textManagement of mandibular fracture using open reduction Internal fixation (ORIF) method is one of the ideal treatments for single and multiple mandibular fractures. The aim of this study was to determine the quality of life of patients after the treatment of mandibular fractures with ORIF using miniplate in single and multiple mandibular fractures. This research used case-control by recalling 43 research subjects in the oral surgery department Dr Sardjito general hospital between 2013 and 2017, that consisted of patients suffering from 23 single fractures and 20 multiple mandibular fractures. Each fracture would be examined clinically and subjectively. Clinical examination parameters were conducted using the mandibular mobility index (MMI) consisting of mouth opening assessment, left and right lateral mandibular excursion, and mandibular protrusive movement. Subjective parameters were performed based on general oral health asseesment index (GOHAI) questionnaire to assess physiological aspects, psychosocial aspects, and pain. The results of the chi-square test statistic study showed that the quality of life of a patient with a single mandibular fracture was better than that of multiple mandibular fractures based on MMI and GOHAI examinations. It was concluded that patients with a single mandibular fracture had a younger age, longer time adaptation and have a better quality of life
The differences in parallelism between ala-tragus line and occlusal plane of down syndrome and normal individuals
No full textPeople with down syndrome have a different craniofacial structure compared to normal individuals. Commonly, down syndrome patients have abnormal ear morphology with smaller size, and lower ear position compared to that of normalsubjects. These patients also have smaller nose, shorter maxilla structure and facial height. Other disorders are anomalies in tooth shape, numbers and size. The ala-tragus line is one of the anatomical landmarks to determine the occlusal plane orientation. Abnormalities in the anatomical structure of people with down syndrome can affect the parallelism between the ala-tragus line and the occlusal plane. The purpose of this research was to determine the differences in parallelism between the ala-tragus line and the occlusal plane of people with down syndrome and normal individuals. The sample in this study were people with down syndrome and normal individuals. The sample was photographed using a digital camera with a fox plane attached to the oral cavity. Parallelism of the ala-tragus line and the occlusal plane was analyzed by tracing these photographs. Photo tracing was done using the Autocad 2013 software. The mean value of angle on people with down syndrome (5.852° ± 5.367°) was greater than that of normal individuals (2.169° ± 2.557°). Based on the research, it was concluded that the parallelism of the ala-tragus line to the occlusal plane in people with down syndrome was different from that in normal individuals. The ala-tragus inferior line in normal individuals was more parallel to the occlusal plane compared to that in people with down syndrome
The effect of platelet-rich fibrin on the acceleration of osteoblast proliferation after endosseous dental implant insertion
No full textThe osseointegration process of dental implants begins with osteoblast differentiation and proliferation, which is an important aspect of the bone regeneration process. Platelet-rich fibrin (PRF) is a platelet that contains a large amount of fibrin and growth factor, which is widely used to accelerate bone regeneration. This study aims to determine the effect of PRF on the acceleration of osteoblast proliferation after endosseous dental implant insertion. Twelve male Wistar rats weighing 250 to 300 gr of 8 to 15 weeks as an animal model were divided into two groups, the control group and treatment group, based on days and PRF treatment. Three ml of Wistar rat blood was obtained and centrifuged for 12 minutes at a speed of 2700 rpm to make PRF. Afterward, platelet-rich fibrin was applied to implant bed, and the dental implant was inserted at the lateral epicondyle of the right femur in 3 mm depth and 1.8 mm diameter dimension. Incontrol groups, dental implants were immediately inserted after implant bed preparation without PRF administration. Observation of the Wistar rats was carried out on days 14 and 28 for each group. The rats were terminated accordingto the timeline of group design. The epicondylus lateralis femoris dextra bone of the rats was taken and fixed with 10% buffered formalin solution. Then, histological samples were made with hematoxylin-eosin staining. Sample observation was done under a light microscope to calculate the number of osteoblasts. The data were analyzed using the two-way ANOVA test, followed by LSD post hoc test. Based on the two-way ANOVA test, there was a significant difference between control groups and treatment groups (p<0.05), and there were significant differences between the observation result of days 14 and 28 (p<0.05). The application of PRF increased osteoblast proliferation for the first 14 days, but the rate decreased after that. Based on the LSD post hoc test, there were differences in osteoblast proliferation between the treatment and control group (p<0.05). Thus, it is concluded that platelet-rich fibrin increased the acceleration ofosteoblast proliferation after endosseous dental implant insertion
Quality of life of patients with lower jaw benign tumor post resection and mandibular reconstruction
No full textThe quality of life of patients becomes an essential part of the success of an intervention since it is used as an outcome measure. Mandible resection will cause discontinuities that can degrade the quality of life of patients. Extensive resectionand reconstruction will affect the quality of life of patients after resection and reconstruction surgery as compared with that before the surgery. On this basis, cross-sectional study was conducted among 27 patients suffering from benignmandibular tumors undergoing surgical procedure of marginal resection, segmental resection, or hemimandibulectomy at Dr. Sardjito genral hospital in 2010-2015. Quality of life of patients after mandibular resection and reconstruction was measured with modified oral health impact profile (OHIP-14). An assessment of the quality of life before and after surgery was based on gender and type of reconstruction and it was analyzed using t-test and one-way ANOVA. It was revealed that the quality of life of patients with benign tumors increased significantly (p=0,000), and that gender differences did not significantly affect the improved quality of life (p=0.433). The mean score of patient’s quality of life was highest on the type of marginal resection (mean value =14.50), and the lowest was on segmental resection (mean value=7.50), but the type of resection did not significantly influence the improved quality of life (p=0.152). Resection and reconstruction procedures under taken by Oral and Maxillofacial Surgeon at Dr. Sardjito general hospital can improve the quality of life of patients with benign tumors of the lower jaw
Effect of immersion time in mouthwash on the flexural strength of polyethylene fiber-reinforced composite
No full textFiber-reinforced composite (FRC) fixed dentures are exposed to various oral environments. One of the ways in maintaining good oral condition is by using mouthwash. Questions have been araised about the safety of prolongeduse of mouthwash towards FRC. The aim of this study was to evaluate the effect of immersion time in mouthwash on the flexural strength of polyethylene FRC. The specimens used were polyethylene FRC (Construct, KerrLab, USA) and flowable composite resin (Master Flow Biodinamica, Brazil). Block shaped specimens (2x2x25 mm) was light cured (n=12). Specimens were divided into threek groups of immersion: Group 1 (without immersion); Group 2 (24 hour immersion); Group 3 (48 hour immersion). The mouthwash used was Listerine Multi-Protect (Johnson & Johnson, Indonesia) (21.6% alcohol content). Flexure strength was measured by Universal Testing Machine. The results showed the average strength value (MPa) for Group 1 was 91.318 ± 12.466, Group 2 was 62.253 ± 8.027, and Group 3 was55.033 ± 3.373. Statistical analysis (ANOVA) showed that immersion time in mouthwash influenced the flexural strength of polyethylene FRC (p<0.05). LSD0.05 showed there were significant differences of flexural strength between Group1-Group 2, Group 1-Group 3 but not for Group 2-Group 3. In conclusion, the flexural strength of polyethylene FRC were decreased by the immersion time in mouthwash
β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure
No full textPanoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients
Effect of sandwich bone augmentation using hydroxyapatite and demineralized freeze- dried bone on infrabony pocket treatment
No full textPeriodontitis is periodontal inflammation in response to plaque bacterial antigens, causing damage to periodontal ligament and alveolar bone resorption. Bone graft material combination i.e. demineralized freeze-dried bone allograft (DFDBA) and hydroxyapatite (HA) using sandwich bone augmentation (SBA) method will support each other and will be beneficial to be used as a scaffold. The body takes long time to resorb HA so this could complement DFDBA which is more easily dissolved. This study aimed to reveal the effect of bone graft addition using SBA method on the treatment of infrabony pocket with open flap debridement in terms of probing depth, relative attachment loss, alveolar bone height, and density. This study was carried out to 20 infrabony pockets, where 10 of them were treated using open flap debridement with HA addition, while the other 10 groups were treated using open flap debridement with DFDBA and HA using SBA method. Probing depth and relative attachment loss were measured on days 0, 30 and 90. Bone height and density were measured using cone-beam computed tomography (images on day 0 and 90). The study showed that probing depth reduction on SBA group was greater than HA group. There were significant differences in probing depth and relative attachment loss examinations. However, bone height and bone density reduction did not show any significant difference. The conclusion from this study is open flap debridement using SBA method yields better regeneration in terms of probing depth and relative attachment loss than open flap debridement with HA addition. There is no difference in bone height and bone density between the two groups