Jurnal Biologi UNAND
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Characterization And Screening Of Protease, Amylase, And Cellulase From Phylloplane Fungi Isolates Of Avicennia marina (Forssk.) Vierh.
Full text linkMangrove (Avicennia marina) is an important mangrove species, because it has many benefits for human life, including in the ecological scope as a source of food and medicine. These plants can also be used as a source of symbiont mold isolates which can be developed as an alternative to produce bioactive compounds, one of which is enzymes. Enzymes are protein compounds that can catalyze all chemical reactions in biological systems. This study aims to determine whether mangrove symbiont fungi (A. marina) can produce amylase, protease and cellulase enzymes. This research was conducted using a method based on enzyme activity, namely amylase activity with lugol iodine staining, protease activity and cellulase activity with congo red staining. on agar media enriched with 1% skim milk, 1% starch and 1% CMC. The results showed that 4 isolates were able to show potential enzymatic activity to be developed as agents for enzyme production
Kidney Histology of Wistar Rats (Rattus norvegicus Berkenhout 1769) Due to White Coffee
Full text linkCoffee is a drink that is very popular with the people of Indonesia and in other countries. Coffee has many benefits but on the other hand, coffee can also damage health, the dangerous content in coffee is caffeine and cafeeic acid. The effect of coffee consumption is still a matter of controversy, one of which is the effect on the kidneys because the kidneys are the main excretory organ for removing metabolic waste from the body. The kidney is the second organ after the liver, which is most often the target of damage by chemicals. This study aims to determine the effect of multilevel doses of white coffee on the histological picture of rats' kidneys. This research is a completely randomized design study with four 4 treatments and four 4 replications. Consists of K (distilled water), P1 (0.36 ml), P2 (1.08 ml) and P3 (2.16 ml). The results of this study showed that macroscopic observations of the administration of white coffee civet coffee solution with different doses showed that the kidneys looked normal. It is characterized by the shape of the kidney like a pea and brownish-red in color. The P0 group was significantly different from the P1, P2, P3 groups. The P1 group was significantly different from the P0 group and the P2 group, P3. The P2 group was significantly different from the P0, P1, and P2 groups. The P3 group was significantly different from the P0, P1, and P2 groups. This study concluded that white coffee in multilevel doses affects the histopathology of kidneys in rats
RAPD Primers Selection for Genetic Variation Analysis of Banana Plant (Musa spp.)
Full text linkIdentification of genetic diversity using molecular markers is important for basic information for plant conservation. Banana is a fruit bearing plants that important for food sources in human life. This study aimed to determine the optimum conditions of  PCR-RAPD reaction and RAPD primers that suitable to amplify DNA fragmens. DNA isolation was done using modified of CTAB and chloroform isoamil alcohol. The samples used was young leaves of nine banana cultivar plants. Optimation was done using variety of DNA and MgCl2 concentration. Eight primers produced by Operon Primer Technology were tested. The DNA genomic concentration obtained was in the range of 23,3 ng/µl – 70 ng/µl. The optimum conditions of PCR-RAPD of banana plants that produce clear band were 50 ng/µl DNA template, 3 mM MgCl2 with the number of thermal cycles was 40 x. There were six RAPD primers that successfully amplified DNA : OPA 02, OPA 04, OPB 12, OPD 20, OPH 01, and OPH 03. The primer OPA-04 had the lowest resolving power value (4,4) , while  OPH 01 had the highest (11,3) resolving power
Diversity of Bivalvia, Gastropoda and Holothuroidea in Intertidal Zone of North Javan Sea Coastal, Indonesia
Full text linkThis study aims to know diversity of Bivalvia, Gastropoda and Holothuroidea. Measures used in this study were Density Index (D), Diversity Index (H’), Evennes Index (E’) dan Important Value Index (INP). This study used transect sampling method by 1 × 1 meter quadrant. Target species of this study were member of Bivalvia, Gastropoda and Holothuroidea that inhabit in intertidal zone of north Javan Sea coastal. This intertidal zone was divided into 3 zone; zone I is 50 meter, zone II is 75 meter and zone III is 100 meter from coastal line to sea. Total sample of this species were 1064 individual, that divided into 7 species of Bivalvia, 10 species of Gastropoda and 2 spesies of Holothuridea. The highest Diversity Index was for Gastropoda in zone I (253 individual/m2). Diversity Index was in moderate category (1< H’ < 2) while the zone III was the highest. Evennes Index of all zones were relatively similar. The highest Important Value Index was species of Clypeomorus clypeomorus, that has value 53,06%
Bioassessment of Batang Kandis River Water Quality Using Macrozoobenthos in Koto Tangah district, Padang City
Full text linkThis study was conducted from July to December 2019 which was aimed to determine the composition of macrozoobenthic community and to assess the water quality of the Batang Kandis river based on the BMWP ASPT index. A survey method was used in this study. The study site was determined by purposive sampling based on environmental condition which was divided into four stations. Macrozoobenthos samples were taken using a surber net (30 x 30 cm2). The physical and chemical factors of water condition were measured during sampling time. The results showed that 23 genera of macrozoobenthic communities were found in the Batang Kandis river which were consisted of two classes i.e., Hirudinea (1 order, 1 family, 1 genus) and Insect (7 orders, 14 families, 22 genera). These macrozoobenthic communities had an average density ranges from 220.00 - 706.67 ind. m-2 with the highest density was found at Station II and the lowest one at Station IV. Variation in the predominant genus was found at every station: Orthocladius and Elophila (station I); Polypedilum, Orthocladius, Elophila, and Hydropsyche (station II); Polypedylum, Macropelopia, and Caenis (station III); and Polypedylum, Orthocladius, and Caenis (station IV). The water quality of Batang Kandis river was classified into not polluted (station I, II and III) to slightly polluted (station IV) based on the BMWP-ASPT indexes.Â
Above Ground Biomass Estimation of Syzygium aromaticum using structure from motion (SfM) derived from Unmanned Aerial Vehicle in Paninggahan Agroforest Area, West Sumatra
Full text linkAbove ground biomass (AGB) is all living organic matters above the soil including stem, seed and leaves. This study aimed to estimate the individual clove (Syzygium aromaticum) and it’s above ground biomass using Unmanned Aerial Vehicle in the Agroforestry area in Paninggahan, West Sumatra. This study used a photogrammetry method to calculate trees and estimated the AGB. We detected 257 numbers of trees based on aerial image analysis and observed 270 after we validated on ground check in the field. The result was slightly different between estimated AGB from UAV and observed AGB from our ground validation. The estimated AGB was 5.9 ton/ Ha where the surveyed AGB was 5.6 ton/Ha. The difference between estimated AGB and observed AGB was 0.3 ton/Ha
Root induction on siam orange (Citrus nobilis Lour.) originated from Kampar using in vitro shoot in MS media enriched with IBA and NAA
Full text linkSiam orange (Citrus nobilis Lour.) is a prominent agriculture commodity in Riau province. It tastes sweet, with fragrant aroma and thin skin. Its cultivation, however, needs improvement to gain better quality of product. One effort that is using the in vitro culture. The study aimed to determine the best IBA and NAA concentrations to trigger root formation on siam orange transplant and used Completely Randomized Design (CRD). The result showed that the rooting reached 100% on all treatments. The fastest rooting was observed in 2 mg/L IBA treatment (7.67 days after planting). The most root growth was obtained with combination of 0.5 mg/L NAA with 1.5 mg/L IBA (2.67 units). The longest root was obtained through the mixture of 1.5 mg/L NAA with 0.5 mg/L IBA (2.27 cm)
Ultrastructure Morphology of Melastoma L. (Melastomataceae) Pollen.
Full text linkMelastoma L. is the type genus of Melastomataceae. While Melastoma malabathricum is the type species with the mostwide spread distribution compared to other species in this genus. This research aim to study ultrastructure morphology of Melastoma pollen. The research was carried out by collecting species which included into Melastoma genus using survey method. Ultrastructure morphology of pollen was observed with Scanning Electron Microscope Type JSM-IT-200. Pollen samples originated from the pre-anthesis Melastoma flowers. The pollen was fixated, dehydrated and coated before observed, photographed and identified with electrone microscope. One species, M. malabathricum, has prolate spheroidal pollen. Four other species, M. setigerum, M. baccarianum, M.minahasae and M. malabathricum var. malabathricum, with oblate spheroidal pollen. The scanning results from electron microscope onto these five Melastoma species observed an aperture like a gap called colpus that varied in number. Pollen ornamentation resemble striate type on these five Melastoma species
Diversity of Ground-foraging Ants (Hymenoptera: Formicidae) in Bukit Kasang and Lubuk Bonta, Padang Pariaman District, West Sumatra
Full text linkThe Study of ground-foraging ant diversity was conducted in Bukit Kasang and Lubuk Bonta, Padang Pariaman District, West Sumatera from April to December 2019. The pitfall trap was used to collect the ground-foraging ants on the area in seven type of habitats. A total 35 species belonging to 23 genera, 4 subfamilies were collected, with Camponotus (4 spp.), Pheidole (3 spp.), and Leptogenys (3 spp.) being the taxa with the most species. Myrmicinae is subfamily with the highest number of species with 15 species, followed by Formicinae (10 spp.), Ponerinae (8 spp.) and Dolichoderinae (2 spp.)
Antimicrobial Activity of Skin Secretion of Rana hosii Frog (Anura: Ranidae) against several pathogenic microbes
Full text linkThe research on antimicrobial activity of skin secretion derived from Rana hosii (Anura: Ranidae) against several pathogenic microbes had been conducted at Microbiology Laboratory and at Genetics and Biomolecular Laboratory, both in the Biology Department, Universitas Andalas. The research used to survey and experimental methods. The study aimed to determine the effectivity of Rana hosii skin secretion to inhibit microbial activity, hence it tested onto some pathogenic microbes such as Escherichia coli, Staphylococcus aureus and Candida albicans. The results showed that the biggest inhibition zone formed against E. coli was 10.96 + 0.63 mm, on S. aureus was 11.74 + 1.00 mm, while on C. albicans was 7.42 + 0.80 mm. The results indicated that the skin secretions from Rana hosii frog could be a potential broad-spectrum antibacterial, and antifungal activity against C. albicans