Jurnal Biologi UNAND
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Optimasi Parsial Isolat Termofilik M5-24 dalam Produksi Protease
Get PDFResearch about partial optimization of thermophilic bacteria isolate M5-24 on protease production was conducted from May 2013 to December 2013 in Laboratory of Microbiology, Biology Department, Andalas University, Padang. Purpose of the research was to find out optimum condition of protease production by isolate M5-24. This study consisted of three steps. Step 1: Investigating the effect of temperature and pH used completely randomized design in factorial with two replications.The first factor was temperature (450C, 500C, 550C, 600C), the second factor was pH (7, 8, 9, 10). Step 2: Investigating the effect of inoculum doses and inducers used completely randomized design in factorial with three replications. The first factor was inoculum doses (1%, 5%, 10%) and the second factor was inducer (yeast extract 0,5%; pepton 0,1%; kasein 1%; meat extract 0,5%). While Step 3: Investigating the effect of agitation (125 rpm, 150 rpm, 175 rpm, 200 rpm) used completely randomized design with six replications. The result showed that the best duration of growing was at 18th hours after incubation, the highest enzyme activity was at temperature 500 C; medium’s pH at 8; the best of inoculum dose was at 5%; the best inducer was casein 1% and optimum agitation was at 175 rpm. Extrinsic optimization can increase protease production of thermophilic isolate M5-24 up to 1.7 times.Keywords : Optimization, Protease, thermophilic, bacteri
Aklimatisasi Planlet Kantong Semar (Nepenthes gracilis Korth.) pada berbagai Campuran Media Tanam Tanah Ultisol
Get PDFThe research about Acclimatization of Pitcher Plant (Nepenthes gracilis Korth.) Planlet on Variety of Mixed Planting Medium Ultisol Soil had been conducted from May to August 2014 in Greenhouse and Plant Physiology Laboratory Department of Biology, Faculty of Natural Sciences, Andalas University. This study aimed to determine the influence of the media composition on the growth of seedlings of Nepenthes gracilis Korth. This study used completely randomized design (CRD) method with 5 treatments and 5 replications. The treatments of planting medium were consisted of A. Ultisol soil (control), B. Ultisol soil : manure : sand (1:1:1), C. Ultisol soil : manure : husk (1:1:1), D. Ultisol soil : manure : sand (2:1:1) and E. Ultisol soil : manure : husk (2:1:1). The results showed that treatment A i.e. ultisol soil (control) was the best growing medium for Nepenthes seedlings growth during acclimatization
Tumbuhan Ficus L. (Moraceae) di hutan konservasi Prof. Soemitro Djojohadikusumo, PT. Tidar Kerinci Agung (TKA), Sumatera Barat
Get PDFThe floristic study of Ficus L. at conservation forest Prof. Soemitro Djojohadikusumo PT. Tidar Kerinci Agung (TKA) West Sumatera have been carried out from September 2012 to January 2013. Samples were directly collected during surveys along the tracks at the study area between 300 and 543 meter above sea levels. The samples were processed and identified at Herbarium ANDA, Biology Department. Twenty spesies of Ficus were identified. Each species could be distinguished by some specific morphological characters such as stipula, leaf frorm, waxy glands, and fruit type. We prepared description for each species and determination key of species level.
Keywords: Ficus, forest conservation, floristic study, morfologycal character, palm oil planation and Species
Pertumbuhan Kunyit Putih (Curcuma zedoaria Rosc.) yang Diinokulasi Fungi Mikoriza Arbuskula Hasil Isolasi Dari RizosfirHornstedtia scyphifera Steud.
Get PDFA study on growth of turmeric (Curcuma zedoaria Rosc.) inoculated with Arbuscular Mycorrhizzal Fungi (AMF) isolated from the rhizosphere of Situngkek (Hornstedtia scyphifera Steud.) was done from March to August 2013 in the wire house and in Plant Physiology Laboratory, Department of Biology, Faculty of Natural Sciences, Andalas University. The aim of this study was to determine the growth of white turmeric inoculated with several doses of AMF. The experiment used Completely Randomized Design (CRD) with five treatments, control (A) and four replications. The inoculation doses were 10 g / Plant AMF (B), 20 g / plant AMF (C), 30 g / Plant AMF (D), 40 g / Plant AMF (E), 50 g / Plant AMF (F). The results showed that AMF inoculation gave no significant effect on plant height, number of leaf, leaf width and dry weight of turmeric. The highest root infection was 30 gr/plant (74.83 %). Generally, turmeric plant have a dependency on FMA inoculation with less and sufficient criteria of Habte and Manjunath.
Keyword: Curcuma zedoaria, Mycorrhizal, Growth
Analisis Vegetasi Gulma Pada Perkebunan Karet (Hevea brasiliensis Mull.Arg.) di Kecamatan Batang Kapas, Kabupaten Pesisir Selatan
Get PDFVegetation analysis of weeds at rubber plantation (Hevea brasiliensis Mull.Arg.) at Batang Kapas, Pesisir Selatan, was carried out from March to April 2013. The weeds were sampled purposively using plot quadrat method at three and six years old of rubber plantations. There were totally 13 families, 23 genera dan 27 species of weeds found in the two types of rubber plantations. The two locations were dominated by different species of weeds. Borreria alata has the highest Summed Dominance Ratio (SDR) at three years and Scleria sumatrensis at six years old of rubber plantations. Diversity indices were relative moderate (2,17 at three years old and 2,2 at six years old rubber plantations) in both locations and species similarity between the two location were low (45,7%)
Inventarisasi Semut Subfamili Formicinae di Kawasan Cagar Alam Lembah Anai, Kabupaten Tanah Datar, Sumatera Barat
Get PDFAn inventory of ants from subfamily Formicinae at Lembah Anai Nature Reserve, West Sumatra was conducted from June to November 2013. Quadra Protocol methods were used to collect ant samples, i.e; hand collecting, honey bait trap, soil core sampling and leaf litter sifting along two 180 m transects. Those methods were applied at two different habitats (forest edge and forest interior). We found 163 individuals of Formicinae which belong to 23 species, 8 genera and 3 tribes. Each species was described based on morphological characteristics. Keywords: ants, Formicinae, identification, Lembah Anai Nature Reserv
Isolasi Bakteri Pendegradasi Limbah Cair Industri Minyak Sawit
Get PDFAn isolation of degrading bacteria of Palm Oil Mill Effluent (POME) was conducted from May to October 2014 at the Laboratory of Microbiology, Department of Biology, Faculty of Natural Sciences, Andalas University. This study aimed to describe the characteristic of the bacteria which can degradate the POME. The result obtained five isolates ie BLS1, BLS2, BLS3, BLS4 and BLS5 and their characters were rod-shaped (basil), gram-positive, motile, positive catalase and forming endospores.                                                                                                        Keywords:      isolation, characterization, degrading bacteria, POM
Dinamika Populasi Anakan Pohon Klimaks Calophyllum soulattri Burm dan Swintonia schwenkii T.&B Di Hutan Bukit Pinang-Pinang
Get PDFA study on population dynamics of Calophyllum soulattri Burm and Swintonia schwenkii T.&B in Pinang-Pinang rain forest have been carried out from September 2012 to Februari 2013 by using descriptive method. Data were collected after 23 years of demographic data available. We found that the highest seedling mortality of Calophyllum soulattri was at the height class of 11-30 cm (9.4 %) and the lowest was at the height class of 71- 80 cm (3.4 %). The heighest seedling mortality of Swintonia schwenkii was  at the height class of 11-20 cm (10.3 %) and the lowest was at height class 51-60 cm (2.9 %). Relative height growth rate of Calophyllum soulattri seedling was at the height class of 21-30 cm (0.38 cm/cm/yr). Whereas relative height growth rate of Swintonia schwenkii seedling was at the height class of 31-40 cm (0.21 cm/cm/yr). Keywords: dynamics population, Calophyllum soulattri, Swintonia schwenkii,mortality, relative high growth rate
Isolasi dan Karakterisasi Bakteri Alkali-Proteolitik Sumber Air Panas Semurup Kerinci Jambi
Get PDFThermophile protease has economic value because this enzyme is useful on all kind of industry that used high temperature on it production process. In the effort of obtaining indigenous alkaline thermostable a protease bacterial that produce we explore potential hot spring at Semurup, Kerinci Jambi. This study was conducted from September 2013 to February 2014, Water samples were collected by purposive sampling, the character of thermophilic bacteria were described for each in laboratory of microbiology and biomolecular, development of biology, faculty of mathematic and natural science Andalas university, Padang. There were 40 thermophile colonies, 7 isolates showed alkali proteolytic index range from 0.3 to 2.00. SIII-10 isolate showed the highest in alkali proteolitic index. The characteristic of SIII-10 are: whute and bolt colony, gram negative, motil, positive sitrat agar and positive gelatin.Key words, Isolation, characterization, alkaline protease, thermostable, Hot spring
Efektivitas Insektisida Komersial Terhadap Kecoak Jerman (Blattella Germanica L.) Strain VCRU-WHO, GFA-JKT dan PLZ-PDG dengan Metode Kontak (Glass Jar)
Get PDFThe purposes of this study was to find out the effectiveness of commercial insecticides to German cockroaches. Study was conducted from September to November 2013 in the Laboratory of Animal Physiology, Department of Biology, Andalas University. This study used the contact method (glass jar) with three strains of German cockroaches (VCRU–WHO, GFA-JKT and PLZ-PDG) and five kinds of insecticide (ByWS, HtWS, MtWS, RdWS, and VpWS). The average of knockdown time and lethal time were counted with a probit analysis. The effectiveness category divided into two groups, value of knockdown time and lethal time were needed to be achieved in a certain time. The results showed that based on the value of knockdown time 90% (KT90), effective aerosol insecticide to knockdown the German cockroach is aerosol insecticide ByWS, HtWS and VpWS. Aerosol insecticide MtWS was only effective to knockdown the German cockroach VCRU-WHO strain and ineffective for GFA-JKT and PLZ-PDG strains. Aerosol insecticide RdWS was only effective to knockdown the German cockroach VCRU-WHO and GFA-JKT strains, ineffective for PLZ-PDG strain. Based on the lethal time 90% (LT90) there was no single insecticide effective to kill German cockroach