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Assessing Speaking & Writing Skills in the EFL Classroom.
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The receptor activator of nuclear factor jB ligand receptor leucine-rich repeat-containing G-protein-coupled receptor 4 contributes to parathyroid hormone-induced vascular calcification
No full textBackground. In chronic kidney disease, serum phosphorus (P) elevations stimulate parathyroid hormone (PTH) production, causing severe alterations in the bone–vasculature axis. PTH is the main regulator of the receptor activator of nuclear factor jB (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) system, which is essential for bone maintenance and also plays an important role in vascular smooth muscle cell (VSMC) calcification. The discovery of a new RANKL receptor, leucine-rich repeat-containing G-proteincoupled receptor 4 (LGR4), which is important for osteoblast differentiation but with an unknown role in vascular calcification (VC), led us to examine the contribution of LGR4 in high P/high PTH–driven VC.
Methods. In vivo studies were conducted in subtotally nephrectomized rats fed a normal or high P diet, with and without parathyroidectomy (PTX). PTX rats were supplemented with PTH(1–34) to achieve physiological serum PTH levels. In vitro studies were performed in rat aortic VSMCs cultured in control medium, calcifying medium (CM) or CM plus 10_7 versus 10_9MPTH.
Results. Rats fed a high P diet had a significantly increased aortic calcium (Ca) content. Similarly, Ca deposition was higher in VSMCs exposed to CM. Both conditions were associated with increased RANKL and LGR4 and decreased OPG aorta expression and were exacerbated by high PTH. Silencing of LGR4 or parathyroid hormone receptor 1 (PTH1R) attenuated the high PTH–driven increases in Ca deposition. Furthermore, PTH1R silencing and pharmacological inhibition of protein kinase A (PKA), but not protein kinase C, prevented the increases in RANKL and LGR4 and decreased OPG. Treatment with PKA agonist corroborated that LGR4 regulation is a PTH/PKA-driven process.
Conclusions. High PTH increases LGR4 and RANKL and decreases OPG expression in the aorta, thereby favouring VC. The hormone’s direct pro-calcifying actions involve PTH1R binding and PKA activation.This study was supported by Instituto de Salud Carlos III (ISCIII)—Fondo de Investigación Sanitaria (FIS 17/00715, FIS16/00637 and FIS 10/00896), Fondo Europeo de Desarrollo Regional (FEDER), Plan de Ciencia, Tecnología e Innovación 2013–2017 y 2018-2022 del Principado de Asturias (GRUPIN14-028, IDI-2018-000152), Fundación Renal Íñigo Álvarez de Toledo and ISCIII Retic REDinREN (RD06/0016/1013, RD12/0021/0023 and RD16/0009/0017 and RD16/0009/0007). Panizo S has been supported by FINBA-REDinREN and IDI-2018- 000152; and Carrillo-López N by GRUPIN14-028 and IDI-2018- 000152, Martinez-Arias L by ISCIII-FINBA (PI16/00637), Alonso-Montes C by ISCIII FINBA-REDinREN and Martín Carro B by ISCIII-FINBA (PI17/00384)
La leche puede contar la vida de la vaca
No full textArtículo divulgativoLoubna Abou El Qassim tiene un contrato de Formación Predoctoral, Programa "Severo Ochoa" de ayudas predoctorales para la investigación y docencia del Principado de Asturias. BP17-049. Organismo financiador: Consejería de Educación y Cultura. Gobierno del Principado de Asturias. Duración: 2019-2021Existen biomarcadores en la leche que pueden servir para trazar el origen y la alimentación del ganado producto
Screen-Printed Electrode-Based Sensors for Food Spoilage Control: Bacteria and Biogenic Amines Detection
Get PDFFood spoilage is caused by the development of microorganisms, biogenic amines, and other harmful substances, which, when consumed, can lead to di erent health problems. Foodborne diseases can be avoided by assessing the safety and freshness of food along the production and supply chains. The routine methods for food analysis usually involve long analysis times and complex instrumentation and are performed in centralized laboratories. In this context, sensors based on screen-printed electrodes (SPEs) have gained increasing importance because of their advantageous characteristics, such as ease of use and portability, which allow fast analysis in point-of-need scenarios. This review provides a comprehensive overview of SPE-based sensors for the evaluation of food safety and freshness, focusing on the determination of bacteria and biogenic amines. After discussing the characteristics of SPEs as transducers, the main bacteria, and biogenic amines responsible for important and common foodborne diseases are described. Then, SPE-based sensors for the analysis of these bacteria and biogenic amines in food samples are discussed, comparing several parameters, such as limit of detection, analysis time, and sample type
Impedimetric Aptamer-based Glycan PSA Score for Discrimination of Prostate Cancer from other Prostate Diseases
No full textProstate specific antigen (PSA) is the common biomarker for prostate cancer (PCa). However, its lack of specificity to differentiate PCa from benign prostate disorders stimulates the search for alternative cancer biomarkers to improve the clinical management of the patients. Different studies have described changes in the core-fucosylation level of PSA between PCa patients and healthy controls. To exploit these findings, we have adapted an impedimetric aptamer-based sensor to the dual recognition of PSA. Two different aptamers, PSAG-1 and anti-PSA, are immobilized onto two adjacent nanostructured gold electrodes. The direct binding from diluted serum samples of specific glycosylated-PSA to the first sensor and total PSA to the second one leads to changes in the charge transfer resistance, which correlate to the amount of glycosylated and total PSA in the sample. The sensors are able to measure PSA in serum with a dynamic range between 0.26 and 62.5 ng/mL (PSAG-1) and from 0.64 to 62.5 ng/mL (anti-PSA), with a reproducibility of 5.4 %. The final output of the proposed platform is the ratio between PSAG-1 reactive PSA and total PSA, defined as the glycan score. The glycan score was tested in serum samples from patients with different pathologies, showing excellent correlation between the measured score and the known diagnosis of the patients. Hence this dual aptamer-based impedimetric biosensor could be used as a minimally invasive method for the diagnosis of prostate cancer.Instituto de Investigación Sanitaria del Principado de Asturias (ISPA
Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling
Get PDFDetecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers. Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging. Here we report a method for selecting aptamers toward the glycosylation site of a protein. It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern. We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event. This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.Instituto de Investigación Sanitaria del Principado de Asturias (ISPA
Protocolo de diagnóstico y manejo de la retención urinaria postparto (RUPP). Hospital Universitario Central de Asturias
No full textProtocolo del Servicio de Obstetricia y Ginecología el HUCA. Área Sanitaria I
Real-Time Impact Analysis and Response using a New Disaster Metrics: 2018 Sulawesi (Indonesia) Earthquake and Tsunami
Get PDFIntroduction: The Richter Scale measures the magnitude of the seismic activity for an earthquake; however, it does not quantify the humanitarian need at the point of impact. This poses a challenge for humanitarian stakeholders in decision and policy making, especially in risk reduction, response, recovery, and reconstruction. The new disaster metrics tool titled “The YEW Disaster Severity Index” (DSI) was developed and presented at the 2017 World Congress of Disaster and Emergency Medicine, May 2017, Toronto, Canada.
It uses a median score of three for vulnerability and exposure indicators, a median score percentage of 100%, and medium YEW DSI scoring of four to five as baseline, indicating the ability to cope within local capacity. Therefore, scoring more than baseline coping capacity indicates that external assistance is needed. This special real-time report was presented at the 2nd National Pre-Hospital Care Conference and Championship,
October 2018, Malaysia.
Report: The aim of this analysis is to present the real-time humanitarian impact and response to the 2018 earthquake and tsunami at Donggala and Palu, Sulawesi in Indonesia using the new disaster metrics YEW DSI. Based on the earthquake (measuring 7.7 on the Richter Scale) and tsunami at Donggala, the humanitarian impact calculated on September 29, 2018 scored 7.4 High in the YEW DSI with 11 of the total 17 indicators scoring more than the baseline coping capacity. The sameYEWDSI score of 7.4 was scored on the earthquake and tsunami at Palu, with 13 of the total 17 indicators scoring more than baseline ability to cope within local capacity. Impact analysis reports were sent to relevant authorities on September 30, 2018.
Discussion & Conclusion: A State of Emergency was declared for a national response, which indicated an inability to cope within the local capacity, shown by the YEW DSI. The strong correlation between the earthquake magnitude, intensities, and the humanitarian impact at Donggala and Palu reported could be added into the science of knowledge in prehospital care and disaster medicine research and practice. As a conclusion, the real-time disaster response was found to be almost an exact fit with the YEW DSI indicators,
demonstrating the inability to cope within the local capacity.Instituto de Investigación Sanitaria del Principado de Asturias (ISPA
Automatic Delineation of Forest Patches in Highly Fragmented Landscapes Using Coloured Point Clouds
Get PDFPrincipado de Asturias - Union Europea
Clarin - Marie Curie Cofund (AC A 17-02
Coordinación sociosanitaria durante la crisis sanitaria por la COVID-19, enfermería y atención a la comunidad.
No full textEditorial de la Revista RqR Enfermería Comunitaria en el que se describe la actuación de las enfermeras como responsables de la coordinación socio-sanitaria en la crisis covid 1