2958 research outputs found
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Apple Pomace, a By-Product from the Asturian Cider Industry, Inhibits Herpes Simplex Virus Types 1 and 2 In Vitro Replication: Study of Its Mechanisms of Action
No full textThe anti-herpes simplex virus type 1 and anti-herpes simplex virus type 2 effects of apple pomace, a by-product from the cider-processing industry, were investigated. The mechanisms of antiviral action were assessed using a battery of experiments targeting sequential steps in the viral replication cycle. The anti-herpetic mechanisms of apple pomaces included the inhibition of virus attachment to the cell surface and the arrest of virus entry and uncoating. Quercitrin and procyanidin B2 were found to play a crucial role in the antiviral activity
Centros Sanitarios Libres de Humo
No full textJornada informativa sobre al Red Asturiana de Centros Sanitarios sin Humo, en el que se define el proyecto y lo que se entiende como centros sanitario sin humo
HUCA libre de humo : Red de Hospitales Libres de Humo del Principado de Asturias
No full textJornada de presentación en el Hospital Universitario Central de Asturias (HUCA) de la Red de HOspitales Libres de Humo del Principado de Asturias, perteneciente a la Red Europea de Hospitales Libres de Humo
Comparative study of PCR-sexing procedures using bovine embryos fertilized with sex-sorted spermatozoa
No full textSex determination in bovine embryos is a useful tool in reproductive biotechnology. This work compares two techniques of embryo sexing by polymerase chain reaction (PCR. Embryos were produced in vitro with sex-sorted sperm and two techniques of DNA lysis were tested (proteinase K versus heat shock). Subsequently, halves of each lysed sample was amplified both by amelogenin and BRY4a/SAT1 primers. Male embryos treated by both digestion methods and amplified by BRY4a/SAT1 gave higher rates of false negatives. Amelogenin amplification failed with embryos previously digested by proteinase K. In contrast, the lysis method allowed obtaining the best accuracy in terms of sex verification when using amelogenin amplification
Genetic resistance to powdery mildew in common bean
No full textPowdery mildew can cause severe yield losses in bean crops. Limited information about resistance sources, and nature and inheritance of resistance are available to bean breeders and plant pathologist. Sources of resistance were searched in seedling tests under controlled conditions in 44 well-known genotypes and in a Spanish germplasm core collection consisting on 201 accessions. A 0–4 scale was used to describe the infection types (IT) observed. Only six out of the 245 evaluated genotypes showed a complete resistance (IT0) without visible symptoms on the leaves: Amanda, Belneb, Cornell 49242, Negro San Luis, Porrillo Sintetico and the local accession BGE003161. Inheritance of resistance was studied in F2 and F2:3 segregating populations. Observed reactions in the five segregating populations fitted to Mendelian ratios with different modes of inheritance. Results revealed that cultivar Porrillo Sintetico carries two dominant and independent resistance genes: one gene conferring complete resistance (IT0), and another gene conferring IT3, characterized by a moderate mycelial development on the leaves. Both genes show a dominant epistatic relationship. Inheritance of response to powdery mildew in cv. Cornell 49242 was similar to cv. Porrillo Sintetico although the correspondence with the genes described in Porrillo Sintetico was not established. Line X2776 carries one dominant gene conferring IT3, and shares this gene with cv. Porrillo Sintetico. In cv. Amanda, two complementary genes appear to be involved in resistance to this fungus. This information will be relevant for the implementation of breeding programs focused on the development of cultivars carrying genetic resistance to powdery mildew
Optimization of seasonality and mother plant nutrition for vegetative propagation of Pinus pinaster Ait
No full textDue to the high economic importance of Pinus pinaster Ait., there is considerable interest in developing, improving and extending the use of its families for mass clonal propagation and in breeding programmes. In the current study, we evaluated shoot growth, rooting ability and mini-cuttings production of P. pinaster in response to nitrogen fertilization and seasons. We compared eight half-sib families of P. pinaster from Asturias and Galicia (Northern Iberian Peninsula), searching for useful parameters and growing conditions to be included in a mass propagation program for clonal family forestry. We fertilized P. pinaster seedling mother plants kept in a greenhouse with three levels of nitrogen: high (HN), medium (MN) and low (LN) to evaluate rooting ability of mini-cuttings. In addition, we evaluated the maximal potential production of rooted mini-cuttings considering nine cycles of propagation over 1 year, also using three levels of nitrogen. The HN treatment significantly influenced the rooting process, with length, area and volume of roots all being positively affected. Spring was the most favourable season for mini-cuttings in the HN treatment. This study provides valuable new information to optimize the clonal propagation protocol for P. pinaster and shows that the mini-cuttings technique has great potential in mass scale cloning, providing high quality sprout production and well-formed new plants
Prion protein gene polymorphism in four West African sheep populations
No full textA total of 162 individuals, belonging to three Burkinabé and one Niger sheep populations, were analysed for prion protein (PrP) gene polymorphism at codons 136, 154 and 171. The ARQ allele was the most frequent in both the Burkinabé (86.7%) and the Niger (67.5%) sheep populations. The highly sensitive allele VRQ was not found in the sampled individuals. The highly resistant ARR allele was in very low frequency in the Burkina-Sahel (4.4%) and Mossi (3.2%) populations and was not present in the Djallonké and Touareg populations. Only 4 out of 15 possible PrP genotypes were identified in the sampled individuals. No favourable ARR/ARR genotypes were found in either of the breeds. Sequencing a subgroup of the samples allowed the identification of other five polymorphisms on the PrP gene sequence at codons 116, 138, 151, 237 and 240. The very low frequency of the ARR allele in the West African sheep should dissuade the implementation of a preventive selection programme aimed to increase resistance to scrapie, to avoid an extreme erosion of the genetic stock
Incidencia de primer ictus y prevalencia de factores de riesgo cardiovascular en el Área Sanitaria II
No full textStroke is the leading cause of disability in adults. In Asturias is the leading cause of death in women and fourth in men. The Health District II is more aging than the set of Asturias -28.3% vs 22.4% people> 65 years in 2011 -. The aim of the study was to determine the incidence of first stroke in the Health Area II and the presence of cardiovascular risk factors prior to strokeXIII Jornadas PASQALEl ictus es la primera causa de invalidez en adultos. En Asturias es la primera causa de muerte en mujeres
y la cuarta en hombres. El Área Sanitaria II tiene mayor envejecimiento que el conjunto de Asturias -28,3%
vs 22,4% las personas >65 años en el año 2011-. El objetivo del estudio fue conocer la incidencia de un
primer ictus en el Área Sanitaria II y la presencia de factores de riesgo cardiovascular previos al ictu
Acoustic communications in water pipes: an experimental approach
No full textArtículo presentado en el "1st International Congress water, waste and energy management" que se celebró en Salamanca los días 23-25 de Mayo de 2012. Dicho artículo recoge las primeras aproximaciones de cara a la consecución de la comunicación digital entre dos puntos usando una tubería de agua como infraestructura y discos piezoeléctricos de bajo coste como sensores de comunicaciónThis paper presents an experimental approach of establishing a digital communication between two points using water pipes as infrastructure and low-cost piezoelectric based sensors.
A pipe network, that represents a common infrastructure used in water distribution, was installed in the laboratory to carry out different set of tests in order to evaluate the best range of frequencies and different digital communication modes. The tests were performed with two low-cost piezoelectric based sensors connected to a laptop computer.
The results of the experimental tests determine that it is possible to establish a reliable communication in the sound range between 2 and 3 kHz. This is considered as the first step for developing of a new family of low-cost devices to remote control the water distribution network."Plan de Ciencia, Tecnología e Innovación (PCTI) de Asturias 2006-2009"
"Programa Operativo Feder del Principado de Asturias 2007-2013"
