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Drug-induced hyperploidy stimulates an antitumor NK cell response mediated by NKG2D and DNAM-1 receptors
Get PDFFormation of polyploid or aneuploid cells is a pathological hallmark of malignant tumors. Cell cycle checkpoint mechanisms play a crucial role in ensuring genomic integrity during mitosis, avoiding the generation of aneuploid cells. Additionally, cancer cell DNA ploidy is subjected to extrinsic controls operated by activation of adaptive immune responses mediated by T cells. NK cells exert a central role in the innate anticancer immunity; however, the mechanisms involved in the recognition of tumor cells by NK cells have not been fully elucidated. Herein, we report that drug-induced polyploidy in cancer cells activates antitumor responses mediated by NK cells. Thus, hyperploidy-inducing chemotherapeutic agents strongly upregulate the tumor expression of ligands for the NK cell activating receptors NKG2D and DNAM-1. Drug-induced hyperploidy modulated the repertoire of activating receptors and the cytokine profile of NK cells, rendering tumor cells more susceptible to NK cell-mediated lysis through the activation of NKG2D and DNAM-1 receptors. In addition, hyperploidization stimulated the production of IL-2 by CD4 T cells, which induced NK cell proliferation and activity. The stimulation of MICA, a key NKG2D ligand, in hyperploid cells was mainly mediated by ATM protein kinase. Likewise, pharmacological inhibition of key regulators of endoplasmic reticulum stress in certain cell models supports a role for this pathway in NKG2D ligand upregulation. Overall, our findings indicate that, besides the cytotoxic effect on tumor cells, the therapeutic activity of anti-mitotic drugs may be mediated by the induction of a coordinated antitumor immune response involving NK and T cells
Broadening the Chemical Scope of Laccases: Selective Deprotection of N-Benzyl Groups
Get PDFEl sistema catalítico formado por la lacasa de Trametes versicolor y el TEMPO es utilizado de forma eficiente para llevar a cabo la desprotección quimioselectiva de los grupos N-bencilo primarios en condiciones suaves de reacción.Laccase from Trametes versicolor together with TEMPO has
been found as a very efficient system to deprotect Nbenzylated
primary amines, differing from previously
described methods since it uses oxygen as mild oxidant in
aqueous medium. Chemoselective removal of the benzyl
group was achieved with excellent yields when secondary
amine and alcohol moieties were also presentMICINN (Projects CTQ2011-24237 and CTQ2013-44153).
Principado de Asturias (predoctoral fellowship Severo Ochoa)
Recursos fitogenéticos de maíz para forraje y grano
Get PDFEl maíz es una de las muchas especies
importadas a Europa tras el descubrimiento
de América. En Galicia y en la
Cornisa Cantábrica se adaptó muy bien a
la climatología y, dado su alto rendimiento,
su explotación se fue extendiendo hacia
toda Europa. Podemos decir que el
cultivo del maíz fue causa y consecuencia
de la revolución industrial de la agricultura.
Aumentó el rendimiento de la superficie
cultivada y permitió la estabulación de
los animales, que empezaron a ser alimentados
con piensos, mientras producían
el estiércol necesario para abonar los
cultivos, constituyendo desde entonces
una parte muy importante de la dieta tanto
humana como animal en Europa
Soil thermal buffer and regeneration niche may favour calcareous fen resilience to climate change
Get PDFThis is a pre-copyedited, author-produced PDF of an article accepted for publication in Folia Geobotanica following peer review. The version of record (Fernández-Pascual, E., Jiménez-Alfaro, B., Hájek, M., Díaz, T. E., & Pritchard, H. W. (2015). Soil thermal buffer and regeneration niche may favour calcareous fen resilience to climate change. Folia Geobotanica 50, 293-301) is available online at: http://dx.doi.org/10.1007/s12224-015-9223-y.Calcareous fens are azonal habitats permanently saturated by groundwater. This is expected to have a buffer effect on soil temperature, alleviating climate changes and allowing plant communities to occupy diverse climatic regions. We analysed the extent of such buffering and its relation with a relevant plant trait, the seed germination niche breadth, along altitudinal gradients in fens of the Cantabrian Mountains (Spain) and the Western Carpathians (Slovakia). In each fen we recorded soil temperature for several years and compared it with WorldClim predictions for air temperature. We also collected seeds from five Cyperaceae fen specialists to evaluate the influence of soil temperature on germination. Although soil temperatures and WorldClim were strongly correlated, their absolute values differed substantially, showing a narrower thermal amplitude and warmer minimum winter temperature in the soil. The greatest differences in soil temperature and germination niche breadth were those between mountain regions. Narrower germination niches correlated with the colder Slovakian winter. Our results suggest that the soil thermal buffer allows species to prevent frost temperatures in winter, but also high summer temperatures in warm regions, explaining their wide distribution ranges. The warm regeneration niche does not match the cooler soils, but shows variability and potential for adaptation. While this findings support resilience to climate warming, changes in precipitation rather than temperature seem to be the main threat for fen persistence.The Masaryk University of Brno provided institutional support. E.F.P. was supported by the Government of Asturias (Grant BP09-107, Programa de Ayudas Predoctorales ‘Severo Ochoa’, Plan de Ciencia, Tecnología e Innovación del Principado de Asturias) and the FP7-Marie-Curie-COFUND programme of the European Commission (Grant ‘Clarín’ ACA14-19); B.J.A. by the project ‘Employment of Best Young Scientists for International Cooperation Empowerment’ (CZ.1.07/2.3.00/30.0037) co-financed by the European Social Fund and the state budget of the Czech Republic; M.H. by the Academy of Sciences of the Czech Republic (RVO 67985939)
Taller de procesamiento de trayectorias cinemáticas de sensores aerotransportados en RPAS
Get PDFLa incorporación de sistemas de posicionamiento autónomo a los sistemas de captura de datos para aplicaciones geomáticas no es una novedad. La integración de sensores estación total/GPS, GPS/Inercial, cámara fotogramétrica/GPS/INS, clinómetros con distanciometría.
La aparición de sistemas aerotransportados no tripulados (UAVs) para aplicaciones geomáticas está originando acercamiento de los sensores aéreos al fotográmetra, permitiendo a este realizar la toma de datos sin la dependencia de una
compañía especializada en navegación aérea. En la actualidad prácticamente la totalidad de los UAV existentes, tanto comerciales como proyectos de código abierto, utilizan receptores GPS con seguimiento de portadora L1 y código C/A. Este tipo de sensores garantizan un posicionamiento por debajo de los 3 m, pero sin la posibilidad de control o postproceso externo. El sensor publica un flujo de datos en formato NMEA o propietario del que solamente se puede extraer posición, tiempo y estado de satélites para cada época, sin posibilidad de postproceso o cálculo diferencial de posición
Estudio de la Impedancia en la Apertura de Guías Abiertas Sintetizadas en Substrato Microstrip (SIW)
Get PDFEste trabajo ha sido financiado por el Gobierno del Principado
de Asturias mediante el Plan de Ciencia Tecnología
e Innovaci´on (PCTI) con las ayudas BP13042 y BP12032,
por el Gobierno del Principado de Asturias y FEDER (Unión
Europea) a través del proyecto GRUPIN14-114 y por el
Ministerio de Economía y competitividad a través del proyecto
TEC2014-54005-P.XXX Simposium Nacional de la Unión Científica Internacional de Radio, URSI 201
Spanish biofuels heating value estimation based on structural analysis
Get PDFThe importance of waste biomass as an energy source is likely to increase during the coming years as a result of European energy policy objectives, and because of the wide range of possibilities that it offers: it is a cheap fuel, widespread, and available in large quantities. In addition to crops and forestry operations, the Spanish fruit, olive and wine industries generate large amounts of currently undervalued solid wastes such as stones, branches, pulps or pomaces. The use of these by-products offers environmental benefits like removing waste and preventing fires at the same time as providing an energy yield. A proper energy valorization will require a complete physicochemical characterization. In this article, a structural and thermal characterization is developed from twenty samples from the olive and wine industries, as well as from forest and agro wastes. In addition, predictive equations are proposed to determine higher heating value (HHV) from chemical composition. For this purpose, the chemical extraction method (also called the ‘classic’ method) was used, and results were obtained in accordance with the data shown in the bibliography. Two predictive equations were developed: one based on lignin and hemicellulose content, and the other based on lignin quantity. Both present an absolute average error (AAE) of 0.87% and 1.13%, respectively.Beca predoctoral Severo Ochoa (BP13058)
PSE-ARFRISOL (PS-120000-2005-1)
MINECO (CTQ2013-45155-R
Applying Goldratt’s Theory of Constraints to reduce the Bullwhip Effect through Agent-Based Modeling
Get PDFIn the current environment, Supply Chain Management (SCM) is a major concern for businesses. The Bullwhip Effect is a proven cause of significant inefficiencies in SCM. This paper applies Goldratt’s Theory of Constraints (TOC) to reduce it. KAOS methodology has been used to devise the conceptual model for a multi-agent system, which is used to experiment with the well known ‘Beer Game’ supply chain exercise. Our work brings evidence that TOC, with its bottleneck management strategy through the Drum-Buffer-Rope (DBR) methodology, induces significant improvements. Opposed to traditional management policies, linked to the mass production paradigm, TOC systemic approach generates large operational and financial advantages for each node in the supply chain, without any undesirable collateral effect.Beca predoctoral Severo Ochoa. Ref BP13011
Programa de Atención al Déficit Auditivo Infantil 2002-2014
No full textPrograma de Atención al Déficit Auditivo Infantil 2002-201
Entre la arcilla y la madera. Reflexiones sobre la pervivencia de formas cerámicas romanas en la tornería tradicional asturiana
No full textI Encontro Internacional de Novos Investigadores en Arqueoloxía e Ciencias da Antigüidade (EINIACA)La vajilla de las casas campesinas asturianas entre el siglo XVI y el XIX era, casi en su totalidad, de madera torneada. Los cuencos y las fuentes de la tornería tradicional asturiana, sobre todo los fabricados en la zona de Ibias recuerdan y tienen una gran familiaridad con algunas formas de cerámica romana que se documentan en Asturias entre los siglos I y II d.C.. Pero… ¿a qué se debe éste hecho