TMKarpiński Publisher
Not a member yet
270 research outputs found
Sort by
Proposal for screening of kidney disease in a random population based on World Kidney Day campaign
Get PDFDespite the advances on early screening techniques, getting to know the chronic kidney disease (CKD) prevalence in Brazil and worldwide remains a challenge for researchers. Aging, diabetes and hypertension are the main CKD causes in Brazil. The aim of the study was to evaluate the presence of urinary dipstick abnormalities in World Kidney Day campaign. This is a cross-sectional studyconducted at FMABC. This study was based on the answers to a kidney disease questionnaire and urinary dipstick test. A total of 205 patients were randomly invited to collect urine samples on World Kidney Day 2013. Among the 205 studied patients, 66.34% were women with mean age of 46.32 years. Around 34.14% of the patients were hypertensive and 9.75% diabetic. Urinalysis alterations were observed in 28.29% of patients. The group with urine alterations had older individuals (51.36 years) andmore diabetes (18.96%) with higher levels of glucose (143.2 mg/dl). Brazilian population is getting older and diabetic which represent risk factors for the onset of CKD. The necessity of an early detection by means of specific campaigns is thus of great importance. The use of dipstick test for screening is an important tool for kidney disease diagnosis.
DOI: http://dx.doi.org/10.5281/zenodo.82692
Raspberry pomace - composition, properties and application
Get PDFRaspberry pomace can be valorised due to its nutritionally favourable effect on human health. It is an important source of polyphenols, ellagic acid, ellagitannins, tocopherols, unsaturated fatty acids, and dietary fibre. Thus, raspberry pomace can be considered as a potential raw material to receive products rich in polyphenols or dietary fibre, which can provide healthy properties to food when used as an additive. This review presents the chemical composition and antioxidant properties of raspberry pomace. The possibilities of its usage in industry are also briefly reviewed.
DOI: http://dx.doi.org/10.5281/zenodo.49519
Efficiency of consortium for in-situ bioremediation and CO2 evolution method of refines petroleum oil in microcosms study
Get PDFAn in-situ bioremediation study was conducted in a laboratory by using mixed microbial consortium. An indigenous microbial consortium was developed by assemble of two Pseudomonas spp. and two Aspergillus spp. which were isolated from various oil contaminated sites of India. The laboratory feasibility study was conducted in a 225 m2 block. Six treatment options: Oil alone, Oil+Best remediater, Oil+Bacterial consortium, Oil+Fungal consortium, Oil+Mixed microbial consortium, Oil+Indigenous microflora. Out of five treatments, the mixed microbial consortium (Block 5) degraded 55.12% refine petroleum oil compare to degradation of bacterial (Block 3) and Fungal consortium (Block 4) (i.e, degradation rate were 19.88% and 18.07% correspondingly) after the end of treatment (60 days). Bioremediation ability of this consortium was confirmed by CO2 evolution method. The result showed that 136.36 % CO2 release after 12 days incubation. 16r DNA sequencing showed that two bacterial species were Pseudomonas aeruginosa and morph taxonomical examination of fungus were Aspergillus terrus (At) and Aspergillus flavus (Af).The ability of degradation of synthetic mixture of refine petroleum oils makes the consortium potentially useful for bioremediation and microbial enhanced oil recovery.
DOI: http://dx.doi.org/10.5281/zenodo.50106
Nodulation and nitrogen fixation of some wild legumes from differing habitats in Egypt
Get PDFThis study was devoted to exploring the natural nodulation and nitrogen fixation of wild legumes grown in different Egyptian habitats. These habitats are representative to four phytogeographical regions. Sites that inhabited by Melilotus indicus, Medicago polymorpha, Trifolium resupinatum, Trigonella hamosa and Vicia sativa in each region were selected for study. High nodulation, nitrogen fixation and plant biomass were recorded in plants grown at Nile region and Oases compared with those at Mediterranean region and Sinai. The inhibition in nodulation and potential of nitrogen fixation in legumes at MR and S were attributed to drought and low soil fertility. Differences in species, regions or their interaction have significant effect on nodulation, legheamoglobin, nitroginase activity and biomass of nodules, shoots and roots; the magnitude of effect due to different species was the greatest. Five rhizobial isolates (Sinorhizobium fredii, Rhizobium mesosinicum, Rhizobium daejeonense, Rhizobium huautlense, Rhizobium alamii) recovered from root nodules of the five species were identified by 16S rRNA gene sequence. The indigenous rhizobia of legumes grown at MR and S expected to be exhibit higher tolerance to the existing harsh environmental conditions. These rhizobia can be used as inoculants for crop legumes under unfavorable environmental conditions of agroecosystems or recently reclaimed desert.
DOI: http://dx.doi.org/10.5281/zenodo.22401
Leaf biomass and leaf area equations for three planted trees in Iran
Get PDFLeaf area (LA) and leaf biomass (LB) are important variables for most physiological, horticultural and agronomic studies involving plant growth, development rate, radiation use efficiency, and water or nutrient use. Measuring these variables need destructive and aggressive sampling. Fortunately, evolving allometric equations can help for low cost and non-destructive estimation of such variables. The aims of this study are Estimate, compare and develop allometric models of LA and LB per tree and per stand for Alnus subcordata (AS), Populus deltoides (PD) and Taxodium distichum (TD) plantations. We selected 12 sample trees in each stand. Leaf Fresh weight of randomly selected branches was weighted in the field. Branch-level LA was modeled as a function of branch diameter (R2 > 0.8) and total fresh weight of LB has been calculated for each sampled tree. For each species, 100 leaves from all canopy directions of trees were randomly selected and transported to the laboratory. At the lab, leaf area has been measured using leaf area meter. Allometric equations were derived using regression analysis. For all species, derived equations showed high accuracy (R2 ranged from 0.837 to 0.947). However, with respect to mean square error, power regression equations (individual leaf area = a(L×W)b and LA or LB = a DBHb) are best models to estimate Individual Leaf Area, LA, and LB of AS, PD, and PD. The highest LAI was in the order of 16.9 > 5.5 > 4.5 for AS, PD, and TD, respectively.
DOI: http://dx.doi.org/10.5281/zenodo.26791
Anthelminthic/larvicidal activity of some common medicinal plants
Get PDFThe helminthic infection are most common disease in different animals and in human beings, which affecting a large proportion of the world population. Helminthic infection can also affect millions of livestock resulting in considerable economic loss in domestic animals. For control of helminthic disease in different part of world are uses synthetic medicines which are very effective in curing helminthiasis, but it’s also causes a number of side effects. The continued uses of synthetic anthelmintic/larvicidal drugs are also causing a major drug resistance problem in several parasitic diseases. The plant derived crude products are less efficient with respect to cure of parasitic diseases but one relatively free from side effect. A large number of medicinal plants are traditionally uses to cure helminthiasis in developing countries. Thus, plant derived drugs are gaining a lot of attention for curing parasitic infection. There are several medicinal plants and their different crude products, organic extracts and active components have been scrutinized for using in various methods in helminthic/larvicidal infection control. The present reviews summarized the use of traditional medicinal plants and their different products further leads to evaluation of new researches.
DOI: http://dx.doi.org/10.5281/zenodo.103681
Natural flavonoids: classification, potential role, and application of flavonoid analogues
Get PDFNowadays, it is assumed that natural flavonoids occurring in fruits and plant derived-foods are relevant, not only for organoleptic properties or technological reasons, but also because of their potential health-promoting effects, as suggested by the available experimental and epidemiological studies. This large group of phenolic plant constituents can be divided into several classes: flavanols, flavanones, flavonols, isoflavones, flavones and anthocyanins depending on the differences in their structures.The beneficial biological effects are also attributed to flavonoid analogues and their metal complexes. These compounds are characterized by antioxidant, pharmacological, anti-inflammatory, anti-allergic, antiviral, anticarcinogenic, as well as therapeutic and cytotoxic properties. Furthermore, they possess a wide range of applications including various fields of industry.
DOI: http://dx.doi.org/10.5281/zenodo.54577
Comparison of biofilm-producing Enterococcus faecalis, Enterococcus faecium, and unusual Enterococcus strains
Get PDFThe present study focused on determining the prevalence of biofilm-forming ability in Enterococ-cus faecalis, E. faecium, and unusual Enterococcus clinical isolates, and comparison of resistance and the prevalence of selected virulence factors among biofilm-positive strains. The ability to form biofilm was detected in 13.3% of E. faecalis, 90% of E. faecium, and 57.1% of unusual Enterococcus strains (p=0.026). All E. faecalis strains were susceptible to β-lactams, while 37.5% of unusual and all E. faecium isolates were resistant to these antibiotics. Resistance to gentamicin was detected in 75% of E. faecalis, 55.5% of E. faecium, and 25% of other strains; resistance to streptomycin in 25%, 83.3%, and 50%, respectively. Analysis of the virulence revealed that the enterococcal surface protein (esp) gene was found in all E. faecium, 75.0% of E. faecalis, and 37.5% of other strains; collagen adhesin gene (ace) in 100%, 25.0%, and 37.5%; and hyaluronidase gene (hyl) in 83.3%, 0%, and 37.5%, respectively. Analysis of the resistance and virulence patterns showed that E. faecium isolates had the greatest variety of virulence and resistance determinants, while the lowest variety was exhibited by unusual strains. These findings indicate that unusual biofilm-producing Enterococcus strains have lower resistance and virulence potency than E. faecalis and E. faecium.
DOI: http://dx.doi.org/10.5281/zenodo.100083
Mycological and enzymatic studies on fresh beef meat sold in Taiz City, Yemen
Get PDFThe mycological analysis of 30 fresh beef meat samples on Czapek’s agar at 7º and 28ºC revealed that, heavily contamination with moulds was observed especially at 28ºC. A total of 234 and 400 colonies ⁄ 450 g meat were collected on both temperatures, respectively. Sixty-seven species belonging to 20 genera were identified. Members of Aspergillus, Mucor, Penicillium and Trichoderma were the most prevalent fungi. At 7°C was highly spoilage by yeasts fungi, while filamentous fungi predominated at 28°C. The ability of the common fungal isolates to produce protease and lipase enzymes revealed that most of them were positive. Among 152 isolates tested, 103 (67.8%) and 96 (63.2%) could respectively produce these enzymes. Because the deteriorative effects of the above fungi, food should be frequently and routinely analyzed. Also, it is essential to store the meat at lower temperature immediately after slaughtering and during transport and storage to reduce or prevent mould growth.
DOI: http://dx.doi.org/10.5281/zenodo.103723
Immunomodulatory and hematological effects induced by diclofenac, ibuprofen or paracetamol toxicity in Swiss albino mice
Get PDFAnti-inflammatory drugs (both COX-2 inhibitors and nonselective non-steroidal anti-inflammatory drugs = NSAIDs), paracetamol and opioid agents are associated with potentially different adverse events with varying degrees of efficacy. The present work was conducted to elucidate the haemato-immunological changes in mice when treated with diclofenac (Diclo), ibuprofen (Ibu) and paracetamol (Para). Mice were intraperitoneally administered with Diclo (7.4 mg/kg and 14.8 mg/kg), Ibu (60 mg/kg and 120 mg/kg) or Para (36.7 mg/kg and 73.4 mg/kg) daily for one month against saline-treated mice served as control. Diclo administration (14.8 mg/kg) caused decrease in RBCs count, Hb content and Hct%, depending on dose toxicity, while paracetamol and ibuprofen treatment showed increase in RBCs count, Hb content and Hct%. Additionally, all tested drugs induced activities of IgM and C-reactive protein in serum and caused perturbations in absolute and relative weight of immune related organs. Further, Diclo and Para treatments reduced levels of IgG in dose dependent manner however, Ibu administration enhanced activities of IgG that was reduced with increasing dose of Ibu. And activities of serum complement component C3 was diminished after administration of tested drugs activating alternative complement pathway. The implication of this research is that long use of diclofenac, ibuprofen or paracetamol may cause immunotoxic and hematotoxic effects in mice; and the dose plus the duration of treatment may augment their toxicity probably due to immune modulatory effects. Further studies are needed to assess the relevance between Diclo, Ibu or Para treatment and immunological and hematological perturbations.
DOI: http://dx.doi.org/10.5281/zenodo.104193