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Evaluation of the effects of chrysin-omega-3 and quercetin-omega-3 fatty acids complexes on proliferation and apoptosis in a-735 melanomas cell line
Get PDFBackground and Aim: Recent studies on the treatment of melanoma, have focused on
designing efficient methods with low side effects. The aim of the present study was to
investigate the effect of quercetin- omega-3 and chrysin- omega-3 complexes on proliferation
and apoptosis in the A-375 melanoma cell line.
Materials and Methods: The cells were cultured and then were treated with different
concentrations of quercetin- omega-3 and chrysin- omega-3 complexes. MTT assay and flow
cytometry were used to evaluate the effects of the above compounds on the rate of cell
proliferation and apoptosis.
Results: Chrysin- omega-3 and quercetin- omega-3 complexes in the experimental
concentrations, inhibited cell growth in a time and concentration-dependent manner. No
significant changes in cell growth rate were observed after 24 and 48 hours of treatment with
0-125μM of chrysin- omega-3 complex. However, concentrations of 150, 175 ,and 200μM
chrysin- omega-3 complex significantly decreased the growth of melanoma cells after 24 and
48h (p <0.05). After 72hours, the inhibitory effect was more potent, so that the inhibitory
effect was also seen in lower concentrations (100 and 125μm). The highest rate of induction
of apoptosis was observed after 72hours (p< 0.05). In regard to quercetin- omega-3 complex,
the results of the study were similar.
Conclusion: Antiproliferative effects of quercetin- omega-3 and chrysin- omega-3 complexes
on melanoma cells are very promising, and suggest these compounds can be considered as
potential therapeutic candidates.
Keywords: Melanoma, Quercetin, Chrysin, Omega-3, Apoptosi
The effect of peers support on fear of hypoglycemia in iranian patients with type 1 diabetes: A clinical trial study
Get PDFBackground: Fear of hypoglycemia is a medical emergency which leads to disrupt individuals' normal lives. Peers support is a hopeful approach to improve diabetes self-care behaviors. This study was conducted to assess the effect of peers support on the fear of hypoglycemia in patients with type 1 diabetes. Materials and Methods: This randomized clinical trial study was performed among 60 patients with type 1 diabetes in Qazvin city from September 2019 to October 2020. Patients were assigned to control and intervention groups using a random method. The data collection tools included demographic characteristics and a standard questionnaire for Hypoglycemia Fear Survey (HFS). Patients in the intervention group were trained by skilled peers for 2 months, but those of the control group only received routine hospital training. The data were analyzed by SPSS version 16 and paired and independent t-test. Results: The scores of the fear of hypoglycemia in diabetic patients in the two groups had no significant statistical difference before intervention (t53= 0.93, p = 0.94). But after the intervention, the independent t-test showed that there was a significant difference between the scores of the fear of hypoglycemia in both groups (t53=-2.13, p = 0.03). Conclusions: Considering the results of the current study, peer support for diabetic patients is an effective way to reduce the fear of hypoglycemia. Therefore, it is recommended using this training method to train diabetic patients. © 2021 Wolters Kluwer Medknow Publications. All rights reserve
ارزیابی کیفیت پرکردگی ترکیب کلسیم هیدروکساید-زینک اکساید در درمان ریشه دندان های قدامی شیری با استفاده از توموگرافی کامپیوتری با دسته اشعه مخروطی: یک مطالعه آزمایشگاهی
Get PDFبررسی ارتباط مقابله مذهبی و حمایت اجتماعی باشدت افسردگی در بیماران مولتیپل اسکلروزیس در قزوین
Get PDFبررسی ارتباط بین سواد مالی و کیفیت زندگی با نقش میانجیگری رضایت مالی در سالمندان بازنشسته شهر قزوین در سال 1399
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The clinicopathologic features of colorectal adenocarcinoma with respect to mismatch repair genes and KRAS status: a retrospective study
Get PDFOBJECTIVE: KRAS and mismatch repair genes are two important molecular markers used in determining prognosis and probability of response to the targeted therapy in patients with colorectal cancer. In this study, the expression status of mismatch repair genes was investigated along with mutation in KRAS gene, and clinicopathologic features were also evaluated in these tumors.
PATIENTS AND METHODS: Clinical and pathological data were collected from 153 known cases of colorectal adenocarcinoma. Expression status of mismatch repair genes was assessed using immunohistochemistry (IHC) technique. Mutation in codons 12 and 13 of KRAS gene was evaluated using pyrosequencing technique. Relationship between clinical and pathologic features of the patients with status of MMR and KRAS markers was also investigated.
RESULTS: A total of 16.3% of tumors lacked expression at least in one of MLH1, MSH2, MSH6, and PMS2 proteins. KRAS was found to be mutated in 37.9% of the patients. Tumors were classified into four categories: dMMR + wild type KRAS (12.9%), dMMR + mutated KRAS (3.9%), pMMR + wild type KRAS (49.6%), and pMMR + mutated KRAS (33.9%). The dMMR tumors mostly had features, such as female gender, proximal location, mucinous production, increased number of lymph nodes, Signet ring cell, and vascular invasion. Compared to pMMR + wild type tumors, pMMR + mutated KRAS tumors were more associated with female gender, proximal location, mucinous component, and lymph node metastasis.
CONCLUSIONS: Results of this study indicated a relationship between MMR status and presence of mutated KRAS with clinicopathologic features of colorectal tumors. Knowledge about these markers along with pathological features can help us to predict metastatic progression and prognosis of the disease
