Qazvin University of Medical Sciences
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رابطه بين فلات زدگي شغلي با تمايل به ترك خدمت پرستاران بيمارستان هاي تحت پوشش دانشگاه علوم پزشكي قزوين با ميانجيگري فرسودگي
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FTIR diferentiation based on genomic DNA for species identifcation of Shigella isolates from stool samples
Get PDFShigellosis is one of the major public health concerns in developing and low-income countries caused
by four species of Shigella. There is an apparent need to develop rapid, cost-efective, sensitive and
specifc methods for diferentiation of Shigella species to be used in outbreaks and health surveillance
systems. We developed a sensitive and specifc Fourier-transform infrared spectroscopy (FTIR) based
method followed by principal component analysis (PCA) and hierarchical clustering analysis (HCA)
assays to diferentiate four species of Shigella isolates from stool samples. The FTIR based method
was evaluated by diferentiation of 91 Shigella species from each other in clinical samples using both
gold standards (culture-based and agglutination methods) and developed FTIR assay; eventually, the
sensitivity and specifcity of the developed method were calculated. In summary, four distinct FTIR
spectra associated with four species of Shigella were obtained with wide variations in three defnite
regions, including 1800–1550 cm−1, 1550–1100 cm−1, and 1100–800 cm−1 distinguish these species
from each other. In this study, we found the FTIR method followed by PCA analysis with specifcity,
sensitivity, diferentiation error and correct diferentiation rate values of 100, 100, 0 and 100%,
respectively, for identifcation and diferentiation of all species of the Shigella in stool samples
Analysis of polychlorinated biphenyls in cream and ice cream using modified QuEChERS extraction and GC-QqQ-MS/MS method: A risk assessment study
Get PDFThe concentrations of six non–dioxin-like polychlorinated biphenyls (NDL-PCBs) were measured
using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) by gas chromatography (GC)
coupled with triple-quadrupole (QqQ) tandem mass spectrometry (MS/MS) method in cream and ice
cream offered in Tehran (Iran). The results showed the limits of detection, limit of quantification
and recovery for the PCB analytes in the ranges 0.04–0.16, 0.132–0.482 ng/g fat, 5.2–9.2 and
95.5–107.2%, respectively. The results showed the mean concentration of total 6NDL-PCBs in
cream (21.634 � 2.18 ng/g fat) was higher than that in the ice cream (12.317 � 1.524 ng/g fat)
samples. The estimated daily intake (EDI) was lower than the tolerable daily intake (TDI < 10 ng/
kg BW/day). Ultimately, the probabilistic model with Monte Carlo simulation (MCS) revealed the
incremental lifetime cancer risk levels (95th) of PCB compounds in ice cream (adults = 1.62E−6
and children = 4.37E−6) and cream (adults = 9.65E−7 and children = 7.57E−6) were lower than
the level of acceptable risk (10−4
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Detection of bacterial agents causing prostate infection by culture and molecular methods from biopsy specimens
Get PDFBackground and Objectives: Prostatitis affects about 16% of men in their lifetime and sometimes leading to prostate
cancer. Bacterial infections are the most common causes of prostatitis. Diagnosis of the causative agents of bacterial
prostate infections plays an essential role in timely treating and preventing secondary complications. This study isolated bacterial infectious agents in patients’ surgical prostate and evaluated them by routine and molecular microbiological
methods.
Materials and Methods: In this cross-sectional study, 72 prostate biopsy specimens were collected from the Orology Departmen of hospitals of Qazvin University of Medical Sciences. All samples were cultured in aerobic and anaerobic conditions. Antibiotic susceptibility test by Kirby-Bauer standard method was performed for all isolated bacteria. In addition, all
isolated bacteria were identified using 16S rDNA PCR and sanger sequencing methods. Also, TaqMan real-time PCR was
applied to detect Ureaplasm aurealyticum, Mycoplasma hominins, and Mycoplasma genitalium.
Results: In conventional culture method, out of 18 positive samples, 15 samples (83.3%) were Gram-negative bacteria and
3 samples (16.6%) were Gram-positive bacteria, containing Escherichia coli (55.5%), Klebsiella pneumoniae (11.1%), Enterobacter cloacae (5.5%), Pseudomonas aeruginosa (11.1%), Staphylococcus aureus (11.1%), and Enterococcus faecalis
(5.5%). The results of molecular identification methods were the same as conventional culture results. Also, four patients
were Ureaplasm aurealyticum, and three patients were positive for Mycoplasma hominis.
Conclusion: Most bacteria isolated from prostate specimens belonged to the Enterobacteriaceae family, especially Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae. Staphylococcus aureus and Enterococcus faecalis were cocci
isolated in the specimens too. Also, Ureaplasma urealyticum, and Mycoplasma hominis were identified in prostatitis.
Keywords: Prostatitis; Pathogens; Enterobacteriaceae; 16s rDNA; Real-time polymerase chain reactio
