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Isolation and Identification of Free Living Amoeba from Patients and Contact Lens Users in Iran
Get PDFBackground: Free-living amoebae (FLA) such as Acanthamoeba spp., are considered as opportunistic and pathogenic protozoans. Acanthamoeba granulomatous encephalitis (AGE) is a serious threat for immunodeficient patients and Acanthamoeba keratitis (AK) for contact lens users.
We aimed to identify the presence of free living amoebae in nasal swabs of patients and contact
lens users in Qazvin, Iran.
Methods: During 2019, 251 nasal and oral swabs (including the pharynx and mouth) were collected from patients with diabetes, AIDS and those under periodic dialysis in Qazvin, Iran. In
addition, 27 soft contact lenses were collected from the participants. Following DNA extraction,
PCR and sequencing were conducted to identify the genotypes of the amoeba. Phylogenetic
analysis of the identified sequences was performed using MEGA 7 software.
Results: A strain of Acanthamoeba belonging to the T3 genotype was isolated from hemodialysis
patients. Two specimens of Acanthamoeba with T3 genotype were isolated from keratitis patients.
Conclusion: The clinicians should pay attention to the possible complication of this organism
because this amoeba is potentially pathogenic for immunocompromised patients. Since the
amoeba is present in environmental resources, the use of contact lenses should be accompanied
by considering proper hygien
PREVALENCE OF SALMONELLASTRAINS ISOLATED FROM INDUSTRIAL QUAIL EGGS AND LOCAL DUCK EGGS, IRAN
Get PDFSalmonella is a worldwide public health issue as one of the reasons for
foodborne illness for humans and animals. Eggs can be a significant source
of this bacterium and the prevalence of salmonellosis. Thus, the control of
contamination by Salmonella has become essential for the consumer. This
study investigates the prevalence, and serotype distribution of Salmonella
isolates recovered from industrial quail eggs and local duck eggs collected
from Qazvin city, Iran, in 2020. In this cross-sectional study, 130 eggs were
collected randomly (including 100 industrial quail eggs and 30 local duck
eggs) from the retail and stores in Qazvin city, Iran. Salmonella was isolated
from eggshells and egg contents using conventional culture methods for
selective isolation of Salmonella and biochemical identification, suspect
colonies confirmed by Real-Time PCR assay for the amplification and
detection of Salmonella using specific primers. A 16.67% prevalence of
Salmonella was observed from duck eggs; however, no Salmonella
recovered from quail eggs. Salmonella was isolated from 0% (0 groups of 6
groups) and 16.67% (1 group of 6 groups) of eggshells and contents of duck
eggs, respectively. Isolates from positive egg samples characterized as S.
Typhimurium. Although Salmonella infection was low in this study,
Continuous monitoring is required to prevent health hazards associated with
poultry products in this area, and the presence of duck eggs can be a public
health problem. The results of this study are essential for the government
