Qazvin University of Medical Sciences

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    10283 research outputs found

    Specific detection of fasciola hepatica and f. Gigantica in infected domesticated animals using high-resolution melting analysis (HRM)

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    Background: It is difficult to make an exact morphological distinction between Fasciola hepatica and Fasciola gigantica. We used High Resolution Melting analysis (HRM) method to differentiate the F. hepatica species from F. gigantica in order to differentiate them. Methods: Overall, 80 adult liver flukes were collected from infected slaughtered animals including cattle, sheep and goats from Lorestan Province, western Iran from Sep 2015 to Aug 2017. Genomic DNA was extracted using commercial DNA extraction kit. The multilocus sequences of mDNA including COX1, COX3 and ND6 were amplified employing real-time PCR & HRM analysis. Specific and universal primer pairs were designed for differentiation Fasciola spp. Results: Universal primers cannot be used to distinguish between these two species, but in the contrary, specific primer pairs of each species could differentiate them properly. Molecular identification using specific primer pairs were consistent. Conclusion: HRM is a simple, fast and reliable method for detecting and differentiating F. hepatica from F. gigantica and can be used for diagnostic and epidemiological purposes

    A system dynamics approach for water resources management with focusing on domestic water demand

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    Background: Due to the complexity and interdependence of water resources, the evaluation of the efficiency of water resources management is difficult. So, it is necessary to use effective modeling methods to study and recognize the feedback loops available in water resources systems. Regarding the importance of domestic water for the human health, the aim of this study was to develop a dynamic model with focusing on domestic water management. Methods: This study was performed using a system dynamics approach. The strategies used in this study include population scenarios (ps), water use education scenarios (wu education), and agricultural water management scenarios (Agriwm). Results: There was a significant relationship between education on water saving in domestic sector and water use per capita. In various scenarios of population growth, domestic water use was declined rapidly with education. According to the obtained results, under “ps3-wu education” scenario, lower population growth, and higher water use education, domestic water use will reach the minimum level, by 2041. The results showed that the proposed scenarios can lead to 60% reduction in domestic water use. Conclusion: With education strategy, water use per capita up to 2024 can reach the optimum level of 100 liters per person per day. However, without education even by 2041, this goal is unattainable. So, sustainable goals can be achieved by adopting education strategy. The developed systematic model can be applied in various conditions. In each study area, the regional water and population data should be provide

    Microleakage comparison of resin modified glass ionomer and OrthoMTA used as a coronal barrier in nonvital teeth bleaching

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    Background and Aim: Common causes of dental discoloration include trauma, drugs, genetic defects, decay, and age. In order to correct discoloration of pulpless teeth, internal bleaching is recommended. The aim of this study was to compare microleakage of resin modified glass ionomer and OrthoMTA used as an intraorifice barrier in non-vital bleaching. Methods: In this experimental study, 36 extracted mandibular premolars were selected and randomly divided into two experimental (n = 16) and two control groups (n = 2). 2mm of OrthoMTA and RMGI cements were placed as intraorifice barriers in the experimental groups. Subsequently, we placed a mixture of sodium perborate and 30% hydrogen peroxide as internal bleaching material into the canal and replaced it every three days. Leakage was measured using pH diffusion method by a digital PH meter. Data were statistically analyzed by using T- independent test and repeated measures and variance analysis (P<0.05). Results: The pH value of the negative control group was as same as the PH of normal saline while the PH value of positive control group was significantly higher than those of other groups. PH values of Ortho MTA and RMGI at the baseline and on the first, sixth and ninth day did not show significant differences with one another, while microleakage of OrthoMTA group was significantly lower on the third day. Conclusions: In general, OrthoMTA had less leakage than RMGI but both materials can be used as suitable barriers for internal tooth bleaching Keywords: Tooth bleaching, OrthoMTA, Glass ionomer, leakag

    A Comparative Investigation of the Microleakage of Biodentine and Mineral Trioxide Aggregate as Coronal Barrier in Nonvital Bleaching

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    Background: Internal bleaching is recommended to correct the discoloration of pulpless teeth. This study aimed to compare the microleakage of biodentine and mineral trioxide aggregate (MTA) used as an intracanal barrier in non-vital bleaching. Methods: An experimental study was performed in which 36 extracted mandibular premolars were randomly divided into two experimental groups (n= 16) and two control groups (n= 2). In experimental groups, 2mm of OrthoMTA and biodentine cement were placed as intracanal barriers. The control groups were prepared similar to experimental groups, except that in positive control group the orifice barrier material was not used and in negative control group the whole root surface was covered with nail polish and orifice barrier material was not used. Subsequently a mixture containing sodium perborate and 30% hydrogen peroxide was placed into the pulp chambers and replaced every three days. Microleakage was measured using a pH diffusion method by digital pH meter. pH was checked before placement and at days 1, 3, 6, and 9 after placement. Data analysis was done in SPSS V24 Results: The pH value in negative control group was similar to that in normal saline group, while the pH value in positive control group was found to be significantly higher than other groups (P< 0.05). Nosignificant differences were seen in pH values between the experimental groups at baseline and days 1 and 6, but pH values of biodentine were significantly higher than orthoMTA at days 3 and 9 (P< 0.05). Conclusions: MTA can be used as an efficient orifice barrier during internal bleaching, specifically in cases where there is probability for cervical root resorption. Keywords: tooth bleaching, mineral trioxide aggregate, dental leakag

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