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Colorimetric-based detection of Ureaplasma urealyticum using gold nanoparticles
Get PDFUreaplasma urealyticum (uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target-specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide-functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of U. urealyticum DNA. A non-cross-linking approach utilising a single Au-nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated. Results from both visual and spectral analyses showed that target–Au-nanoprobe hybrids were stable against aggregation after adding the inducer. Furthermore, when a non-target PCR product was used, the peak position shifted and salt-induced aggregation occurred. The assay's limit of detection of the assay was 10 ng with a dynamic range of 10–60 ng. This procedure provides a rapid, facile and low-cost detection format, compared to methods currently used for the identification of U. urealyticum
Glibenclamide mitigates cognitive impairment and hippocampal neuroinflammation in rats with type 2 diabetes and sporadic Alzheimer-like disease
Get PDFA growing body of evidence suggests that type 2 diabetes (T2D) is a risk factor for cognitive impairment and dementia. Both preclinical and clinical studies have provided evidence that brain insulin resistance is associated with cognitive decline in patients with T2D and sporadic Alzheimer disease (AD). Accordingly, antidiabetic medications have been suggested as potential drugs for the treatment of cognitive impairments in patients with sporadic AD. This study set out to determine whether glibenclamide (GBC), an antidiabetic agent, can ameliorate cognitive impairments in rats with T2D and sporadic AD. Both animal models were treated with GBC for 23 consecutive days. To assess working and spatial memory, animals were subjected to the Y-maze and Morris water-maze tests. We measured glucose and insulin levels in the blood, and inflammatory cytokines such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in the hippocampus of animals. Our findings indicated that T2D and sporadic AD impaired memory and elevated TNF-alpha and IL-6 in the hippocampus. We found increased glucose and insulin levels in the blood of T2D-induced rats but not of sporadic AD rats. In contrast, GBC treatment improved memory impairment, increased insulin, and reduced glucose and hippocampal inflammation in rats with T2D and sporadic AD. This study suggests that GBC could be considered as a potential treatment for cognitive deficits in patients with T2D and sporadic AD. Taken together, this study highlights the need for further studies in humans to test whether GBC treatment is associated with cognitive improvement in sporadic AD patients
Effects of unsaturated fatty acids (Arachidonic/Oleic Acids) on stability and structural properties of Calprotectin using molecular docking and molecular dynamics simulation approach
Get PDFCalprotectin is a heterodimeric protein complex with two subunits called S100A8/A9. The
protein has an essential role in inflammation process and various human diseases. It has
the ability to bind to unsaturated fatty acids including Arachidonic acid, Oleic acid and etc.,
which could be considered as a major carrier for fatty acids. In this study we aimed to
appraise the thermodynamics and structural changes of Calprotectin in presence of Arachidonic acid/Oleic acid) using docking and molecular dynami simulation method. To create
the best conformation of Calprotectin-Oleic acid/Arachidonic acid complexes, the docking
process was performed. The complexes with the best binding energy were selected as the
models for molecular dynamics simulation process. Furthermore, the structural and thermodynamics properties of the complexes were evaluated too. The Root Mean Square Deviation and Root Mean Square Fluctuation results showed that the binding of Arachidonic acid/
Oleic acid to Calprotectin can cause the protein structural changes which was confirmed by
Define Secondary Structure of Proteins results. Accordingly, the binding free energy results
verified that binding of Oleic acid to Calprotectin leads to instability of S100A8/A9 subunits
in the protein. Moreover, the electrostatic energy contribution of the complexes (Calprotectin-Oleic acid/Arachidonic acid) was remarkably higher than van der Waals energy. Thus,
the outcome of this study confirm that Oleic acid has a stronger interaction with Calprotectin
in comparison with Arachidonic acid. Our findings indicated that binding of unsaturated fatty
acids to Calprotectin leads to structural changes of the S100A8/A9 subunits which could be
beneficial to play a biological role in inflammation process
Thermodynamic, kinetic and docking studies of some unsaturated fatty acids-quercetin derivatives as inhibitors of mushroom tyrosinase
Get PDFInhibition of activity and stability structure of mushroom tyrosinase (MT) is highly
important, since it is a key enzyme of melanogenesis playing various roles in organisms. In this study,
thermodynamic stability and diphenolase activities were investigated in the presence of
quercetin-7-linoleate (ligand I) and quercetin-7-oleate (ligand II) on mushroom tyrosinase by
experimental and computational methods. Kinetic analyses showed that the inhibition mechanism of
these ligands is reversible and competitive manner. The inhibition constants values (KiI = 0.31 and KiII
= 0.43 mM) and the half maximal inhibitory concentration (IC50 = 0.58 and 0.71 mM) were
determined for ligand I and ligand II respectively. Thermal denaturation for the sole and modified
enzyme were performed by using fluorescence spectroscopy to obtain the thermodynamic parameters
of denaturation. Type of interactions and orientation of ligands were determined by molecular docking
simulations. The binding affinities of the MT–ligand complexes during docking were calculated. In the
computational studies performed using the MT (PDBID: 2Y9X) from which tropolone was removed,
we showed that the ligands occupied different pockets in MT other than the active site. The best
binding energies with values of −9 and −7.9 kcal/mol were calculated and the MolDock scores of the
best poses with the lowest root mean square deviation (RMSD) were obtained as −172.70 and −165.75
kcal/mol for complexes of MT–ligand I and MT–ligand II, respectively. Computational simulations and
experimental analysis demonstrated that the ligands increased the mushroom tyrosinase stability by
reducing the activity of enzyme. In this regard, ligand I showed the potent inhibitory and played an
important role in enzyme stabilit
مقایسه تاثیر آموزش تغذیه خانواده محور وفردی برکاهش علائم جسمی سندروم پیش از قاعدگی در دختران 14-18 ساله
Get PDFEffects of ear and body acupressure on labor pain and duration of labor active phase: A randomized controlled trial
Get PDFObjectives: This study sought to compare the effects of multi-point ear and body acupressure on labor pain and the duration of labor active phase. Design: Three-armed randomized controlled trial. Setting: Kowsar Hospital, Qazvin, Iran. Intervention: Participants in the body acupressure group received acupressure on GB21, GB30, BL32, LI4, and SP6 points, each for two minutes, at cervical dilation of four, six, and eight centimeters. For participants in the ear acupressure group, adhesive auriculotherapy-specific Vaccaria seeds were attached to their auricles on the zero, genitalia, Shen Men, thalamic, and uterine 1 and 2 acupoints. The seeds were compressed every thirty minutes, each time for thirty seconds. Participants in the control group received routine care services. Main outcome measures: Labor pain intensity was assessed using a visual analogue scale at cervical dilation of four and ten centimeters. Results: While there was no significant difference between mean scores of pain among three groups, mean score of labor pain in both acupressure groups was significantly less than that in the control group (P < 0.001). However, the difference between the acupressure groups was not statistically significant (P=0.12). Moreover, the duration of labor active phase in the ear acupressure group was significantly less than those in the body acupressure and the control groups (P < 0.001). Conclusion: Ear acupressure was significantly effective in reducing labor pain and shortening labor active phase. However, body acupressure solely reduces labor pain. Therefore, ear acupressure can be used to reduce labor pain and shorten labor active phase
Assessing the psychological response to the COVID-19: A response to Bitan et al.“Fear of COVID-19 scale: Psychometric characteristics,reliability and validity in the Israeli population”
Get PDFاثرات تورم خدمات سلامت بر رفاه خانوارهای استان قزوین: رویکرد سیستم معادلات تقاضا (AIDS) (فایل دفاع نهایی)
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