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Design of a gold nanoprobe for the detection of Pseudomonas aeruginosa elastase gene (lasB)†
Get PDFNosocomial infections are one of the major health problems that increase mortality. Pseudomonas aeruginosa is one of the important causes of nosocomial infections. This bacterium has a gene called the lasB gene, the product of which is a zinc-dependent metalloprotease. This gene plays a central role in the pathogenesis of Pseudomonas spp. This pathogen is highly toxic and destroys tissues and also has a moderating effect on the immune system; on the other hand, it initiates the intracellular pathway of biofilm growth. Although there are methods such as molecular methods for identifying the lasB gene, due to the high cost and the need for specialized personnel, it is necessary to replace them with an appropriate method. In this study, a gold nanoparticle-based DNA diagnostic sensor sensitive to the aggregation states of gold nanoparticles was used to identify amplified and non-amplified lasB genes. The results of the experiment were evaluated both visually and spectrally. The minimum detection value of this method was 10 ng of the amplified lasB gene and 50 ng of the non-amplified lasB gene. This method is very fast, simple, easy and low cost
ANTICANCER EFFECT OF PROBIOTIC SACCHAROMYCES BOULARDII SUPERNATANT ON HUMAN CACO-2 CELLS; AN IN VITRO STUDY
Get PDFColon cancer is an important worldwide cause of death in human which is treated commonly by chemotherapy, radiotherapy and surgery methods with different side effects. Natural treatment such as microbial cell wall extract is suggested to be used as an effective alternative of chemical drugs for treatment of colon cancers without any side effect. Saccharomyces boulardii is used in probiotic foods and supplement capsules in viable and yeast cell wall extract forms. At the present study, we in vitro investigate the anticancer properties of S. boulardii supernatant (SBS) on colon cancer cells. We found that, SBS without dilution after 72 hours successfully killed the colon cancer cells. Also, this treatment induced apoptosis and downregulated the expression of survivin gene significantly. However, effects of SBS without dilution after 24 and 48 hours were considerable. Downregulation of survivin gene expression by functional compounds in SBS induced apoptosis and killed the colon cancer cells successfully. However, future in vivo and in vitro investigation of anticancer effects of SBS on other cancer cells are suggested to be implemented
2019درباره کوروناویروس جدید
No full textاسلایدهای آموزشی درباره کروناویروس جدید شناسایی شده در چین، به صورت پاورپوین
تاثیر آموزش مبتنی برتئوری رفتار برنامه ریزی شده برعملکرد ایمن تکنسین های فوریت های پزشکی در مواجهه با حوادث شیمیایی(فایل دفاع نهایی)
Get PDFPrevalence of Intestinal Parasites among Rural Inhabitants of Fouman, Guilan Province, Northern Iran with Emphasis on Strongyloides stercoralis
Get PDFBackground: Intestinal parasitic infections (IPIs) are among the most important etiologies of gastrointestinal disorders in developing countries. The present study was performed to determine the prevalence of IPIs in rural inhabitants of Fouman, northern Iran.
Methods: Overall, 31 villages were randomly selected during 2015-2016. Stool samples were collected from 1500 inhabitants aged 2-87. The samples were examined by direct wet smear, formalin ethyl-acetate concentration and agar plate culture. Trichrome staining and modified acid-fast staining were used as confirmatory tests for intestinal amoeba and flagellates and cryptosporidium spp., respectively. Data were analyzed with Chi-Square and Fisher exact tests using SPSS.
Results: 8.06% of participants were positive for at least one intestinal parasite. The prevalence of mixed parasitic infections was 0.87%. The most prevalent IPIs were caused by Trichostrongylus spp. (3.13%), followed by Strongyloides stercoralis (1.5%), Giardia lamblia (1.3%), and Entamoeba coli (1.0%), Blastocystis hominis (0.86%), E. histolytica/dispar (0.53%), Endolimax nana (0.26%), Iodamoeba butschlii (0.13%), Trichuris trichiura (0.07%), Enterobius vermicularis (0.07%), Hook worm (0.07%) and E. hartmani (0.07%). Statistically, the prevalence of IPIs showed significant differences regarding the age groups, education status, occupation (P<0.001), and the habit of eating raw vegetables (P<0.007), whereas, the differences were insignificant with regard to sex (P=0.924) and water supply (P=0.088).
Conclusion: The prevalence of IPIs, especially soil-transmitted helminthes (STHs) has sharply decreased in northern Iran. Excluding Trichostrongylus spp. and S. stercoralis, other intestinal parasites only produce a marginal and unnoticeable health problem in this area, today
Comparison of Intelligence Quotient in Early Treated Neonates with Congenital Hypothyroidism Compared to Healthy Children
Get PDFAssisted reproductive technology and the risk of gestational diabetes mellitus: a systematic review and meta-analysis
Get PDFBackground: The use of assisted reproductive technology (ART) is increasing worldwide, and observational studies
have indicated that women who conceived by ART have an increased risk of pregnancy complications including
gestational diabetes mellitus (GDM). We aimed to determine the risk of GDM among women who conceived with
ART by systematic review and meta-analysis.
Main text: A systematic literature search was conducted in ISI Web of Knowledge, MEDLINE, Scopus, and Embase
through May 2017 for English-language articles using a list of keywords. All studies comparing GDM in women
conceived by ART and those who conceived spontaneously were included. Data extraction was performed by two
authors independently and discrepancies were resolved by discussion. In total, 48 studies with 91,487 pregnancies
conceived through ART and 2,525,234 spontaneously conceived met the inclusion criteria. There was evidence of
substantial heterogeneity among these studies (P < 0.001, I
2 = 98.6%). Random effects meta-analysis showed a
significant increase in GDM among those who conceived by ART compared with those who conceived
spontaneously (pooled relative risk = 1.51, 95% confidence interval = 1.18–1.93). Visual inspection of the funnel plot
did not reveal any publication bias, which was supported by Egger’s test and Begg’s test.
Conclusion: The findings of this systematic review indicate that the use of ART treatment is associated with a 1.51-
fold increase in GDM. Women need to be counselled carefully before undergoing ART treatment about the
possibility and risk of GD
Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population
Get PDFBackground: Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low
back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the
inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play roles
in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single
nucleotide polymorphisms (SNP) in this disease.
Method: Seventy-six patients who were diagnosed with IVDD and 140 healthy controls who complied with
eligibility criteria were included. A total volume of 5 cc peripheral blood was obtained from each participant to
investigate the IL-2 + 166G/T, IL-2 -330G/T, IL-12 − 1188A/C, and IFN-γ +847A/T SNPs through PCR-SSP method.
Results: The ‘TG’ and ‘TT’ genotypes of IL-2 − 330G/T polymorphism were significantly more common among
patients and healthy controls respectively. The ‘GT’ and ‘TT’ haplotypes of IL-2 (comprised of -330G/T, and + 166G/T
SNPs) were also more common among patients and controls respectively.
Conclusion: This study indicated the significant role of IL-2 genotypes and haplotypes in IVDD. These SNPs were
differently distributed in patients and controls. Therefore, alteration in the structure of IL-2 gene could play an
important role in pathophysiology of IVDD.
Keywords: Intervertebral disc degeneration, Single nucleotide polymorphism, Interleukin 2, Interleukin 12, Interferon
γ, Immunogenetics, Cytokin