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KRAS, NRAS, BRAF, and PIK3CA mutation rates, clinicopathological association, and their prognostic value in Iranian colorectal cancer patients
Full text linkAim: Mutations in KRAS, NRAS, BRAF, and PIK3CA genes are critical factors in clinical
evaluation of colorectal cancer (CRC) development and progression. In Iran, however,
the data regarding genetic profile of CRC patients is limited except for KRAS exon2
and BRAF V600F mutations. This study aimed to investigate the mutational spectrum
and prognostic effects of these genes and explore the relationship between these
mutations and clinicopathological features of CRC.
Method: To achieve these objectives, mutations in KRAS (exons 2, 3, and 4), NRAS
(exons 2, 3, and 4), PIK3CA (exons 9 and 20), and BRAF (exon 15) was determined using
PCR and pyrosequencing in a total of 151 patients with colorectal cancer.
Results: KRAS, BRAF, NRAS, and PIK3CA mutations were identified in 41%, 5.96%,
3.97%, and 13.24% of the cases, respectively. There were some significant correlations between clinicopathological features and KRAS, PIK3CA, BRAF, and NRAS mutations. Mutations in KRAS and PIK3CA were shown to be independent risk factors for
poor survival of the patients at stage I-IV (p < 0.0001 and p = 0.001, respectively). No
significant impact on prognosis was observed in patients with BRAF mutations.
Conclusion: Our study revealed the prevalence of CRC biomarkers mutations in
Iranian patients and emphasized the role of KRAS and PIK3CA on shorter overall survival rates in this population
Molecular Characterization of Mitochondrial Cytochrome c oxidase subunit 1 (Cox1) gene from Trichostrongylus Species (Nematoda: Trichostrongylidae) in Northern Iran
Full text linkObjective: The objective of the present study was to identify Trichostrongylus species by molecular analysis and also phylogenetic
relationships of Trichostrongylus species by mitochondrial Cytochrome c oxidase subunit 1 (Cox1) gene in Guilan province,
northern Iran.
Methods: Abomasum and duodenum contents of 144 livestock were collected from sheep, goats, and cattle in Guilan province.
Morphological survey was performed for initial screening. Total DNA was extracted, and the partial region of Cox1 gene was
amplified and sequenced. Genetic diversity was calculated and phylogenetic analysis of the data on nucleotide sequence was
conducted by MEGA7 software.
Results: Three species of Trichostrongylus including T. colubriformis, T. vitrinus, and T. axei were identified by morphological
characteristics. The genetic divergence within the species in the present study was observed for T. axei (0-2.5%), T. colubriformis
(0.77%), and T. vitrinus (0%). The mean inter-species difference between the three species of Trichostrongylus obtained in this
study was 14.4-15.4%.
Conclusion: The Cox1 sequences of the members of Trichostrongylus spp. were highly variable and this could be used as a valuable
measure to achieve a proper assessment on biodiversity. Sequence data generation from other species of Trichostrongylus will be
needed to reconstruct the phylogenetic relationships of this genus of nematodes.
Keywords: Trichostrongylus spp., ruminants, Cox1, phylogenetic analysis, Ira
Evaluation of Biofilm Formation, Hydrolytic Eenzymes and Antifungal Susceptibility of Planktonic Cells of Candida Albicans Species Isolated from Different Clinical Samples
Full text linkBackground: Candida species are common organisms in human and animal mucosa that cause a wide range of
Candida infections in immunocompromised patients. This study investigated the ability to produce proteinase,
phospholipase and hemolysin as well as biofilm formation in different clinical isolates of Candida albicans.
Methods: In this study, ninety-four C. albicans were identified using phenotypic tests and amplification of the
hyphal wall protein (HWP1) gene, and the proteinase, phospholipase and hemolysin production in specific
mediums, as well as the ability to biofilm formation using the crystal violet method were evaluated. Then, the
antifungal susceptibility of planktonic cells was tested on the basis of the CLSI- M27-A3/S protocol.
Findings: In this study, the proteinase, phospholipase and hemolysin activities of C.albicans isolated from
different body sites were 82%, 75.5%, and 68%, respectively. Additionally, 74.5% of the isolates had the ability
to biofilm formation. Among the isolates being studied, the strains isolated from the oral cavity showed the
highest activity of proteinase, hemolysin and biofilm formation, and the strains isolated from vaginal secretions
showed the highest level of phospholipase activity. The susceptibility pattern of C. albicans species to
antifungals showed that all isolates were sensitive to AMB and VRC, and resistance to FLC and ITC was
reported as 5.4% and 2.2%, respectively.
Conclusion: The results show the importance of molecular epidemiology studies and understanding the role of
hydrolytic enzymes and biofilm production in C. albicans strains.
Keywords: Candida albicans; Antifungal agents; Disease susceptibility; Virulence; Biofilm
Epidemiological study of patients admitted to the psychosomatic ward in Qazvin BuAli Sina Hospital in 2019
Full text linkBackground: Psychosomatic disorder is defined as diseases for which there is no medical justification despite the physical symptoms, or despite the physical problem, the patient's complaint is much more than the severity of the disease. The aim of this study is to investigate the epidemiological and demographic characteristics of patients hospitalized in the psychosomatic department of Bo Ali Qazvin Hospital in 2018 .
Method: This study is a cross-sectional descriptive study on 150 patients who were admitted to the psychosomatic department of Bo Ali Hospital in 2018. Therefore, for patients, a questionnaire including: gender, age, place of residence, education, employment status, previous history of psychiatric illness, hospitalization history, family history of psychiatric disorder, history of referral to a psychiatrist, history of medical illness, recent psychiatric illness, how the illness started, Drug use and associated diseases were recorded through the patients' files.
Results: Out of this number, 57.33% of the patients were female, against 42.67% were male. Also, the highest accumulation of frequency in terms of age group was in the group of 31-40 years old with a percentage of frequency of 30.67%. 70% of the patients were married, 27.33% were single, and 2.67% were separated. In terms of employment status, 54.67% of housewives and 11.34% were unemployed. 59.34% of the patients were below diploma in terms of education. 74% of the patients did not have a history of hospitalization due to psychiatric disorders. 67.33% of the patients did not have stressful situations such as unemployment, divorce, co-morbidities, arguments and death of close relatives.
Conclusion: The findings of the present study show 54% of the studied patients had a history of psychiatric disorder, and 8% of these patients had psychosomatic disorders. Also, a high prevalence of psychiatric disorders was observed in women, 57.33%.
Key words: epidemiology - psychosomatic - demography - psychiatric disorde
بررسی پارامترهای پاتولوژیکی در بیماران مبتلا به کلون کنسر با کاهش میزان بیان Circ-0006282
Full text linkEvaluation of Cannabidiol (CBD) effects on positive symptoms of patients with Schizophrenia in 22 Bahman hospital of Qazvin city in 1399
Full text linkچکیده
زمینه و هدف: اسکیزوفرنی یک اختلال روانپزشکی با دوره های مداوم یا عودکننده روان پریشی میباشد . دارو های آنتی سایکوتیک خط اول درمان اسکیزوفرنی, از طریق آنتاگونیست گیرنده های مرکزی دوپامین هستند. مطالعه ای با هدف بررسی تاثیر کانابیدیول به عنوان داروی کمکی در بیماران اسکیزوفرنی تحت درمان با ریسپریدون بر اساس پرسشنامه ی PANSS و مقایسه با گروه دریافت کننده ی ریسپریدون و پلاسبو در بیمارستان 22 بهمن قزوین سال 1401 انجام دادیم.
مواد و روش ها: این مطالعه کارآزمایی بالینی سه سوکور, بر روی 30 بیمار اسکیزوفرنی مراجعه کننده به بیمارستان 22 بهمن قزوین در سال 1401انجام شد. بیماران به دو گروه مداخله با رسپریدون و 150 میلی گرم CBD لیپوزومال در روز یا رسپریدون و دارونما تقسیم شدند. قبل از شروع درمان, دو هفته , 4 هفته و6 هفته بعد از شروع درمان پرسشنامه ی PANSS (positive and negative syndrome score) برای بیماران تکمیل گردید و تغییرات بررسی شد. داده ها با استفاده از نرم افزار SPSS وارد کامپیوتر شد و سطح معنی دار کمتر از 0.05 در نظر گرفته شد.
یافته ها: فاکتورهای میانگین نمره ی کل پرسشنامه ی PANSS ، میانگین نمره ی علائم مثبت و میانگین نمره ی علائم جنرال بین دو گروه کانابیدیول و پلاسبو درهفته های 2,4و6 بعد از مداخله تفاوت معناداری وجود دارد و بین میانگین نمره علائم منفی طی روند درمان 6 هفته ای بین 2 گروه تفاوت معناداری وجود ندارد. هم چنین در دو گروه مورد مطالعه تفاوت معناداری از نظر بروز عوارض وجود نداشت.
نتیجه گیری: طبق این مطالعه میتوان از کانابیدیول در کنار داروی ریسپریدون به عنوان دارو ی کمکی جهت درمان بیماران اسکیزوفرنی استفاده کرد. بهبود بهتر و سریع تردر گروه دریافت کننده ی کانابیدیول به نفع استفاده از این دارو است.
کلمات کلیدی: اسکیزوفرنی_ کانابیدیول_کانابینوئید_ PANSS_سایکوز_CB
بررسی فراوانی لنگش و علل ان متعاقب درمان شکستگی دیافیز شفت فمور با میله داخل استخوانی در سال 1400 در بیمارستان شهید رجایی قزوین
Full text linkEnhancement of immune responses by vaccine potential of three antigens, including ROP18, MIC4, and SAG1 against acute toxoplasmosis in mice
Full text linkToxoplasma gondii (T. gondii) causes considerable financial losses in the livestock industry and can present serious
threats to pregnant women, as well as immunocompromised patients. Therefore, it is required to design and
produce an efficient vaccine for controlling toxoplasmosis. The present study aimed to evaluate the protective
immunity induced by RMS protein (ROP18, MIC4, and SAG1) with Freund adjuvant, calcium phosphate nanoparticles (CaPNs), and chitosan nanoparticles (CNs) in BALB/c mice. The RMS protein was expressed in
Escherichia coli (E. coli) and purified using a HisTrap HP column. Thereafter, cellular and humoral immunity was
assessed by injecting RMS protein on days 0, 21, and 35 into four groups [RMS, RMS-chitosan nanoparticles
(RMS-CNs), RMS-calcium phosphate nanoparticles (RMS-CaPNs), and RMS-Freund]. Phosphate buffered saline
(PBS), CNs, CaPNs, and Freund served as the four control groups. The results displayed that vaccination with
RMS protein and adjuvants significantly elicited the levels of specific IgG antibodies and cytokines against
toxoplasmosis. There were high levels of total IgG, IgG2a, and IFN-γ in vaccinated mice, compared to those in the
control groups, especially in the RMS-Freund, indicating a Th-1 type response. The vaccinated and control mice
were challenged intraperitoneally with 1 × 103 tachyzoites of the T. gondii RH strain four weeks after the last
injection, and in RMS-Freund and RMS-CaPNs groups, the highest increase in survival time was observed (15
days). The RMS can significantly increase Th1 and Th2 responses; moreover, multi-epitope vaccines with adjuvants can be a promising strategy for the production of a vaccine against toxoplasmosis
بررسی شیوع ابتلا به بیماری کووید-19و ارتباط آن با رعایت پروتکل های بهداشتی در صنایع شوینده شهرستان البرز
Full text linkچکیده:
هدف:پاندمی کووید19دولت ها و تصمیم گیرندگان حوزه سلامت را با چالش های زیادی مواجه کرده است. با توجه به شیوع گسترده و عدم وجود درمان موثر، یکی از بهترین راه های پیشگیری، رعایت شیونامه های بهداشتی است. ویروس کرونا در محیط های جمعی به سرعت انتشار می یابد از اینرو، کارگران شاغل در صنایع مستعد ابتلا به ویروس کرونا هستند. باتوجه به تراکم جمعیت در کارخانه ها و شرکت ها، بررسی شیوع کووید19و نیز بررسی وضعیت رعایت شیوه نامه های بهداشتی مقابله با کرونا ضروری است. مطالعه حاضر با هدف بررسی شیوع ابتلا به بیماری کووید19و ارتباط آن با رعایت شیوه نامه های بهداشتی در صنایع شوینده شهرستان البرز انجام شد.
روش کار: این مطالعه مقطعی در 7 واحد صنعتی فعال در حوزه تولید محصولات شوینده شهرستان البرز با استفاده از روش نمونه گیری طبقه ای تصادفی نسبی انجام شد با در نظر گرفتن سطح اطمینان95%، سطح خطای مجاز 3% و شیوع11% حجم نمونه با نرم افزار stata ، 552 نفر محاسبه شد اطلاعات مربوط به ابتلای کووید19 و اطلاعات دموگرفیک براساس پرسشنامه اطلاعات دموگرافیک جمع آوری شد و میزان رعایت پروتکل های بهداشتی با استفاده از چک لیست مصوب وزارت بهداشت ارزیابی شد برای توصیف داده های کمی از میانگین و انحراف معیار و برای توصیف داده های کیفی از تعداد و درصد استفاده شد. آنالیزهای تحلیلی به روش رگرسیون لجستیک تک متغیره و چند متغیره با استفاده از نرم افزارSPSS )نسخۀ21) انجام شد.
یافته ها: میانگین سنی شرکت کنندگان 27/7±17/35 سال و3/14% زن بودند میزان رعایت پروتکل های بهداشتی به صورت میانگین 6±36/94 درصد در هریک از صنایع بود و شیوع بیماری کرونا در صنایع تولید مواد شوینده 5/33 % بود. نتایج نشان داد شیوع کرونا باسطح تحصیلات، سابقه ابتلای خانواده، حضور در اماکن شلوغ و گندزدایی سطوح ارتباط دارد.(05/0>p)
نتیجه گیری: توسعه زیرساخت های بهداشتی و تقویت اقدامات کنترل مهندسی بهداشت حرفه ای در شرایط عادی، نقش اساسی در آمادگی برای مقابله با شرایط اضطراری و پیشگیری و کنترل اپیدمی ها در صنایع دارد.
کلیدواژه ها: کووید-19، پروتکل های بهداشتی، بهداشت حرفه ای، صنایع شویند