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Sero-Epidemiological Studies of Trypanosoma in Cattle and Buffalo Using Card Agglutinationtest (CATT) in West Java, Indonesia
No full textPenelitian sero-epidemiologik pada surra telah dilakukan di Jawa Barat. Sample darah dikumpulkan dari 237 ekor sapi dan 48 ekor kerbau dari 5 Koperasi Unit Desa(KUD) sapi perah/sapi potong di Bogor, Sukabumi serta sample darah kerbau dari rumah pemotongan hewan (RPH) Bogor. Darah diperiksa terhadap parasit trypanasoma dangan metoda "microhacmatocrit" (MHTC) dan sampel serum diperiksa atas adanya antibody terhadap T.evals dengan :Card Agglution Test" (CATT). T.evals dideteksi positif dengan MHCT pada 1,3 % dari sapi yang diperiksa dan 2,1% pada kerbau, sedang antibody positif dideteksi 10% dari sapi dan 66,7% dari kerbau.Persentase hewan yang positif terhada T.evansi adalah rendah, tetapi pemeriksaan serologik adalah relatif tinggi. Diduga bahwa keadaan ini adalah kronis pada populasi hewan
The experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respectively better than FLS (32.9%). The fertilization rate was significantly higher (p
No full textThe experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respectively better than FLS (32.9%). The fertilization rate was significantly higher (
Conception Rate In Ewes Having Different Number of Ovulating Follicle During The First Estrous Cycle After PGF2, Injection
Get PDFForty sis ewes were used to study the effects of number of ovulating follicle on the conception rate during the first estrous cycle after PGF& injection. The experimental ewes were injected twice with PGF& to synchronize estrous cycle and to remove previously existed corpus luteum. The number of ovulating follicle was determined by counting the number of corpus luteum by laparoscopy, 5 days after the estrous cycle. The distribution of ewes having 1. 2. 3, and >3 ovulating follicle was 12, 19, 12, 3, respectively. Conception rate in each group during the first estrous cycle after the last PGF& injection was determined by counting the parturition date. The results of the experiment showed that the hgher the number ofovulating follicle the greater the percentage of ewes being pregnant (33,33, 63,12, 58,33, and 10%, for ewes having 1, 2, 3, and >3, ovulating follicle, respectively). It was suggested that an increasing number of ovulating follicle could be used to improve conception rate in ewes
Portal Blood Flow Of Femgle Growing and Lactating Goats on Different Food Intakes
Get PDFNet production or uptake rates of the portal drained viscera (PDV) were estimated by measuring portal venous blood flow (PBF) according to the Fidc principle in asmiation with measurement of arterio-venous blood concentration differences. H-paro-aminohippuric acid wes constantly infused into a mesenteric vein to measure PBF on female growing and lactating PE goats fed different levels of feed intakes. Cardiac output (CO) and whole body energy expenditure were measured by the carbondioxidee entry rate technique (CERT). PBF (n=2 for each group) of growing goats were 1032,624,652,486 and 394 mllmin for 100,90, 80,70 and60 % of ad libitum feeding which corresponded to 32.7, 17.4, 19.8, 14.8 and 13.7 % of CO. For lactating goats, PBF (n-2 each) were 500, 371 and 223 ml/min for 100, 90 and 80 % of ad libitum feeding, corresponding to 13.6, 13.7 atid 7.6 % of CO. The calorigenic effect associated with feed intake of growing goats varied in descending order from 45 to 20 % of total body energy expenditure, while that of lactrrting goats was around 7 %. Absorption of volatile fatty acids was proportional to level of dietary intake
Morphology Of The Gut Endocrine Cells In The Gastrointestinal Tract Of The Lesser Mouse Deer (Tragulus javanicus)
Get PDFThe ultrastructure of endocrine cells in the gastrointestinal tract of the lesser mouse deer (Tragulus javanicus), the smallest ruminant, was studied using electron microscopy. The cells possess components such as rough endoplasmic reticulum, golgi complexes and mitochondria and characterized prominently by the presence of cytoplasmic secretary granules. The secretary granules were polymorphous, rounded, oval or spindle shaped and varied greatly in size and electron density from one cell type to another. The granules were generally concentrated in the intranuclear region of the cells. Two types of endocrine cells could be observed. Open type cells were oval, triangular or spindle in shape, showed apical lurninal contact by means of microvilli. Closed type cells were generally round or triangular in shape. The endocrine cells were located in the basal portions, close to either capillaries or submucosal nerve fibers. The morphology of the endocrine cells was discussed in relation to their possible functions
The research was conducted for 12 months at three different locations of poultry farm at Kemang of Parung sub district, Bogor. The sites are about 10 km away from Bogor, surrounded by orchards or other trees and far enough of human dwelling. The influence of ambient temperature, humidity, monthly rainfall and number of rainy days were observed against the population of filth flies Musca domestica and Ophyra chalcogaster. Within a year the four climatic factors during wet and dry seasons were not significantly different (p>0.05), and the influence of climate was not distinct against the population of filth flies and parasitoid. However, the population of parasitoid was strongly correlated of filth flies (r=0.921).
No full textThe research was conducted for 12 months at three different locations of poultry farm at Kemang of Parung sub district, Bogor. The sites are about 10 km away from Bogor, surrounded by orchards or other trees and far enough of human dwelling. The influence of ambient temperature, humidity, monthly rainfall and number of rainy days were observed against the population of filth flies Musca domestica and Ophyra chalcogaster. Within a year the four climatic factors during wet and dry seasons were not significantly different (p>0.05), and the influence of climate was not distinct against the population of filth flies and parasitoid. However, the population of parasitoid was strongly correlated of filth flies (r=0.921)
In Vitro Development Of Holstein And Japanese Black Breeds Embryo
Get PDFTwo experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulatedcow serum (SCS) and fetal calf serum (FCS) supplementation into medium for in vjtro development (experiment I) and the effect of different source of oocytes (experiment II). Theendpoint was development to cleavage on day 2 and to blastocyst up to day 9 after msermnabon. In experiment I, oocytes were matured and cultured m vltro (after fertilization)in TCM-199 supplemented with 5% SCS or 5% FCS. The cleavage rate of zygotes cultured in medium supplemented with 5% SCS was higher (
Milkchecker, As An Alternative Tool To Detect Subclinical Mastitis
Get PDFTwo hundred and thirty milk samples of different quarters taken from dairy cows around Bogor and Cipanas were used in this study. The milk samples were tested aseptically against subclinical mastitis using the MC and AMP in the field and in the laboratory. The amount of leucocytes were counted using the BREED method.Using the MC the sensitivity is 85.2% and the specificity is 97.8% in detecting subclinical mastitis. In comparing the use of MC to the AMP or to the BREED method no significant difference was found. The MC should be used routinely to detect mastitis especiallynew for cases of subclinical mastitis
The Evaluation Of Potasium Thiocyanate Extracted Pasteurella multocida Vaccine On Pig
Get PDFPasteurella multocida has been isolated from pigs at Kapuk Farm West Jakarta that had been diagnosed pasteurellosis. These isolates were extracted with potassium thiocyanate(KSCN) and has been evaluated as vaccine for pig. Seventeenth 3-4 years-old pigs were used for experiment and they were divided to four groups. i.e (i) control, (ii) vaccination, (iii)vaccination and challenge and (iv) challenge groups. All pigs were fed and receive water ad Irbiturri. The experiment was carried out for 30 days. All experiment groups showed no difference statistically for antibody titer until days-21. At days-23, vaccination groups showed antibody titer higher than non vaccination groups.Challenge groups showed clinical sign i.e. anorexia, abdominal respiration, rhinitis and demonstrated pathological changes i.e. local dermatitis superficialis, limphadenitis, oedem cervicalis ventral, trcheitis, laryngitis, pleuropneumoni, emphysema pulmonum, pleuritis, pericarditis, epicarditis, hipertrophy ventricle dextra, dilatation ventricle sinistra, splenitis, anteritis catarhalis, tephlitis, colitis, cystitis,, myositis necroticans, gastritis catarhalis and gangrenosa, degeneration parenchymatosa. hidronephrosis, sinusitis catarhalis,pneumoni lobuler and epiglositis. Histopathological changes showed alveoli damaged, germinal ccnters formation in spleen and timpltoglandulae which was more severe in thechallenge groups. P. multocida was isolated from visceral organs that showed pathology and histopathology changes.Mild clinical signs was observed in the vaccination groups. This groups showed similar pathological changes as other groups but therc were no d e m a at cervicalis region. This group showed chronic histopathological changes. No P. nrultocido was isolated from visceral organs. It can be concluded that all vaccinated pigs was protected by vaccine during the 30days experiment
The Application of Decomposting Microbes For Reduce Amonia and Hydrogen Sulfide Emission From The Piggeries in Bali
Get PDFThe unpleasant odor of piggeries is mostly caused by the emission of ammonia and hydrogen sulfide. An experimentwas conducted to study the effect of decomposting microbes EM4 on the emission of ammonia and hydrogen sulfide. The manure was treated with four different concentrations of EM4@, 0\u2770, 0.5%, 1.0% and 1.5% respectively. The ammonia released was trapped in 0.02N boric acid and analyzed with Nessler method, while the hydrogen sulfide was trapped in 0.02N zinc acetate and analyzed with the methylene blue method. The study suggested that the high concentrationof ammonia and hydrogen sulfide gas were decreasedafter 14 days, and there was no significant differencesin either ammonia or hydrogen sulfide emission although theammonia emission decreased to under 20 ppm with of 1.5%EM^" and the hydrogen sulfide decreased to 0.202 ppmwith application of 1.0% EM4@