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Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve.
Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve
Effect Of Manure Input On Growth And Dry Matter Production of Napiergrass (Pennisetum purpureum Schumach) and Sorghum (Sorghum bicobr Moench)
Pertumbuhan dan produksi bahan kering Rumput Gajah (Pennisetum purpureum Schumach) dan Sorgum (Sorghum bicolor Moench) pada pemberian tiga tingkat pupuk kandangtelah diteliti selama lima bulan. Total pupuk kandang segar yang digunakan pada tiap tingkat pemupukan adalah 250 tonha (pemupukan tinggi), 150 tonha (pemupukan sedang) dan 10 tonha (pemupukan rendah). Produksi bahan kering, tingkat perhmbuhan tanaman dan indeks luas permukaan daun pada Rumput Gajah cenderung meningkat dengan rnenhgkatnya pemberian pupuk kandang, namun tidak berbeda nyata antara pemupukan tin& dan sedang. Produksi bahan kering, tingkat pertumbuhan tanaman dan indeks luas permukaan daun pada Sorgum berbeda nyata diantara perlakuan dan cenderung meningkat dengan meningkatnya pemberian pupuk kandang. Hubungan antara indeks luas permukaan dam dan tingkat pertumbuhan tanaman adalahlinier positif, tingkat pertumbuhan tanaman cenderung meningkat dengan meningkatnya indeksluas permukaan daun
Pendekatan Metodologi Dalam Penelitian Metabolisme Pada Ruminansia
Climatic factor of high temperatures and humidities of humid tropical countries, alongside with the occmence of diseases and parasites have created a complex situation in terms of the effect on animal production. Studies on quantitative relationships for better understanding of digestion, metabolism and related areas are lacking. There are however a number of appropriate methodologies available for conducting onfarm metabolic research aiming at giving better understanding of animal production and health in the humid tropics. The paper discusses methodologies and experimental approaches developed in the authors\u27s laboratory on growing,draft pregnant and lactating ruminants. Several examples of the results are presented
Sample of eggs, feed, rice grains and DOD taken from center for traditional hatcheries of Alabio ducks in Mamar Village District of Hulu Sungai Utara South Kalirnantan were examined for Aspergillus contamination. The result showed that @40% of hatched eggs, 25-50% of feed, 0% of rice grair\s and 032.86% of DOD was contamined The species of Aspergillus found in those materials were Aspergillus flavus (18 isolates), Aspergillus fumigatus (12 isolates), Aspergillus niger (9 isolates) and AspergiUus sp. (9 isolates). Aspergillus content in feed sample examined was in the range between 2.0 X 103 - 2.3 X 103 colonies/gram.
Sample of eggs, feed, rice grains and DOD taken from center for traditional hatcheries of Alabio ducks in Mamar Village District of Hulu Sungai Utara South Kalirnantan were examined for Aspergillus contamination. The result showed that @40% of hatched eggs, 25-50% of feed, 0% of rice grair\s and 032.86% of DOD was contamined The species of Aspergillus found in those materials were Aspergillus flavus (18 isolates), Aspergillus fumigatus (12 isolates), Aspergillus niger (9 isolates) and AspergiUus sp. (9 isolates). Aspergillus content in feed sample examined was in the range between 2.0 X 103 - 2.3 X 103 colonies/gram
The Pathological Chabges of Male Goat Infected by Haemonclus contortus (Rudolphi, 1803)
Pathological aspects of abomasum, intestine, liver, spleen, heart , bone marrow and lymphnode caused by H. contortus were examined. Twelve bucks. six to eight month of ages were divided into 4 groups. which were infected by 0 (DO). 200 (D200). 400 (D400). and 600 (D600) h r d stage larvae of H. contortus. Larvae was administered orally 3 times a week for 8 weeks. Patological aspects including macro and microscopic findings were studied for enteritis, abomasitis, extramedullary haemopoietic centre forming, germinal centre activity of spleen and lymphnode and activity of bone marrow
Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG
The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embry
Semen Production in Sambar Stag and Response to Oestrus Synchronization in Sambar Hind
Studies on the production of spermatozoa and oestrus synchronization in sambar deer (Cervus unicolor) were conducted using nine stags and 12 hinds. Prior to the semen collection via electroejaculator stimulation, stags were sedated using a combination of fentanyl citrate, azaperone and xylazine hydrochloride (Fentazin®, Parnell Lab. NZ). Collected semen were evaluated for its volume, motility and concentration. Collected semen were evaluated for its volume, motility and concentration, placed into straw and then kept in frozen, chilled and fresh container. In hinds, the synchronization was conducted using intravaginal Controlled Internal Drug Release type G (CIDR-G®, 9 % w/w, 300 mg progesterone; Agricultural Division, CHH Products Group Ltd, Hamilton, NZ), followed by i.m injection of 250 IU PMSG. The results showed that stags and hind did not responp very well to all treatments. Mean of ejaculated semen volume was low (0.91 ml) and some ejaculated semen had 80 % motility. Any semen collection should be conducted based on the individual performance, with the age time in hard antler condition as the main factors. Half of the hinds responded to the synchronization 59.5 hrs after the injection of PMSG
Three dimensional architecture of the sub epithelial connective tissue in the forestomach of the lesser mouse deer was studied by scanning electron microscopy after macerated with 10% NaOH. In general, the architecture of the connective tissue in the rumen and reticulum showed similar pattern, which was honeycomb like pattern. This pattern was observed in all portions of the forestomach. Primary wall bordered each cell of the honeycomb. Inside the cells there were some secondary or tertiary wall that connect to the primary ones. The primary wall in the rumen were leaflike shaped with narrow and irregular surface while those of the reticulum were low columnar shaped with convex surface. The honeycomb in the ruminal papillae showed similar pattern from the apical to the basal portion. On the contrary, cone-like primary wall without secondary or tertiary ones dominated the apical portion of the reticulum papillae. The sub epithelial connective tissue consisted of collagen fibers which were arranged and formed a network. The collagen fibers in the rumen were more densely distributed as compared to those of the reticulum. The differences observed may indicate a difference in function between the rumen and the reticulum in the digestive function of the lesser mouse deer.
Three dimensional architecture of the sub epithelial connective tissue in the forestomach of the lesser mouse deer was studied by scanning electron microscopy after macerated with 10% NaOH. In general, the architecture of the connective tissue in the rumen and reticulum showed similar pattern, which was honeycomb like pattern. This pattern was observed in all portions of the forestomach. Primary wall bordered each cell of the honeycomb. Inside the cells there were some secondary or tertiary wall that connect to the primary ones. The primary wall in the rumen were leaflike shaped with narrow and irregular surface while those of the reticulum were low columnar shaped with convex surface. The honeycomb in the ruminal papillae showed similar pattern from the apical to the basal portion. On the contrary, cone-like primary wall without secondary or tertiary ones dominated the apical portion of the reticulum papillae. The sub epithelial connective tissue consisted of collagen fibers which were arranged and formed a network. The collagen fibers in the rumen were more densely distributed as compared to those of the reticulum. The differences observed may indicate a difference in function between the rumen and the reticulum in the digestive function of the lesser mouse deer
The objective of this research was to investigate the genetic and phenotypic correlations for several productive traits on Madura cattle. The results were expected as a fundamental consideration in selection programme. The research was conducted in Bangkalan Madura for a year. Hundred and eighty calves were analysed from 9 sires sampled using stratified random analysis. At the end of the research 3 bulls were sold thus in the final analysis 120 calves from 6 sires were analysed using resemblance between relatives. The results indicated that genetic and phenotypic correlations between birth weight and weaning weight were 0.43±0.31 and 0.32±0.18, respectively. Genetic and phenotypic correlations between birth weight and pre weaning gain were 0.38±0.28 and 0.32±0.18, respectively. Genetic and phenotypic correlations between weaning weight and yearling weight were 0.59±0.11 and 0.31 ±0.27, respectively, and finally genetic and phenotypic correlations between weaning weight and post weaning gain were 0.43±0.13 and 0.47±0.24, respectively. Based on the correlations, a selection will be able to be conducted based on birth and weaning weight.
The objective of this research was to investigate the genetic and phenotypic correlations for several productive traits on Madura cattle. The results were expected as a fundamental consideration in selection programme. The research was conducted in Bangkalan Madura for a year. Hundred and eighty calves were analysed from 9 sires sampled using stratified random analysis. At the end of the research 3 bulls were sold thus in the final analysis 120 calves from 6 sires were analysed using resemblance between relatives. The results indicated that genetic and phenotypic correlations between birth weight and weaning weight were 0.43±0.31 and 0.32±0.18, respectively. Genetic and phenotypic correlations between birth weight and pre weaning gain were 0.38±0.28 and 0.32±0.18, respectively. Genetic and phenotypic correlations between weaning weight and yearling weight were 0.59±0.11 and 0.31 ±0.27, respectively, and finally genetic and phenotypic correlations between weaning weight and post weaning gain were 0.43±0.13 and 0.47±0.24, respectively. Based on the correlations, a selection will be able to be conducted based on birth and weaning weight
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells
Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut