Jurnal Agripet
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Addition of Sodium Hyaluronate to Ringer Lactate-Egg Yolk Diluent on the Quality of Native Chicken Sperm
Full text linkThis study aims to evaluate the effect of adding varying concentrations of sodium hyaluronate (SH) to a Ringer lactate-egg yolk diluent on the quality of native chicken spermatozoa during 48 hours of storage. A completely randomized design (CRD) was used with four treatment groups: control (diluent without SH), diluent with 63 M SH, 103 M SH, and 143 M SH. The study involved six replications with pooled semen from three native chickens aged approximately 10 months. Semen was collected, diluted, and stored at 5C for 48 hours. Parameters observed after storage included motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity. Data were analyzed using analysis of variance (ANOVA). The results indicated that the treatments had no significant effect (P0.05) on any of the spermatozoa quality parameters. The observed values for motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity were 46.67-50%, 95.11-96.55%, 92.77-95.55%, 4.77-5.44%, and 71.05-75.37%, respectively. In conclusion, the addition of sodium hyaluronate at concentrations of 63-143 M in a Ringer lactate-egg yolk diluent does not significantly affect the quality of native chicken spermatozoa during 48 hours of storage at 5C
Meat Quality Comparison in Bali, Wagyu, and Their Cross-Breed Cattle Using Ultrasound Imaging
Full text linkThis study aimed to compare the meat quality of different beef cattle breeds using ultrasound imaging. A total of 28 cattle, aged 1-2.5 years, from three breeds were analyzed: Wagyu (n=7), Bali (n=10), and WagyuBali cross-breeds (n=11). Meat quality traits, including longissimus dorsi thickness (LDT), backfat thickness (BFT), intramuscular fat (IMF), and marbling score (MS), were assessed using ultrasound imaging. The association between breed and meat quality was analyzed using a completely randomized design (CRD) followed by Tukey's test. Additionally, principal component analysis (PCA) was employed to identify clusters of meat quality potential among the different breeds. The results indicated that WagyuBali cross-breeds exhibited the highest LDT (46.3804.770 mm), though the difference was not statistically significant (P0.05) compared to either Bali or Wagyu cattle. However, significant differences (P0.05) were observed between Bali and Wagyu cattle. For BFT, MS, and IMF, Wagyu cattle outperformed both Bali cattle and WagyuBali cross-breeds, with values of 5.4900.806 mm, 6.0100.998, and 49.058.140%, respectively. The PCA revealed two primary clusters: the first cluster, comprising Wagyu cattle, accounted for 75.6% of the diversity and was characterized by BFT, IMF, and MS as key variables. The second cluster included Bali cattle and WagyuBali cross-breeds, representing 21.5% of the diversity, without any specific meat quality variable as a defining marker. Ultrasound imaging effectively estimated meat quality in Bali cattle and their cross-breeds, demonstrating its potential as a tool for meat quality assessment across different breeds
Chemical Quality (Water Content, Protein, Fiber) and Organoleptic Properties of Chicken Nuggets with Addition of Vegetables
Full text linkThis study aimed to assess the chemical quality (moisture content, protein, and fiber) and organoleptic properties of chicken nuggets with the addition of vegetables. The materials used included chicken breast, wheat flour, tapioca flour, bread crumbs, eggs, pepper, salt, ice water, carrots, broccoli, spinach, and young corn. Five treatments were applied: P0 (no vegetable addition), P1 (carrot addition), P2 (broccoli addition), P3 (spinach addition), and P4 (young corn addition). The research employed a Completely Randomized Design (CRD) for analyzing chemical quality and a Randomized Block Design (RBD) for examining organoleptic properties. Preferences were tested using the Kruskal-Wallis test. The chemical quality measurements included moisture content, protein, and crude fiber, while the organoleptic measurements assessed color, aroma, texture, taste, and preference. The results showed that the addition of vegetables significantly (P0.05) affected moisture content but had no significant effect (P0.05) on protein and fiber content. Furthermore, the addition of vegetables significantly (P0.05) influenced the color, aroma, and taste of the nuggets, but did not significantly affect (P0.05) texture and preference. It was concluded that incorporating 10% vegetables into chicken nuggets increased moisture content without affecting protein and fiber levels or overall preference. The addition of carrots yielded the highest scores for aroma and taste preference
Relationship of Chest Girth, Body Length, and Sex with Body Weight and Average Daily Gain in Batur Lambs
Full text linkThis study aimed to investigate the relationships between chest girth, body length, and sex with body weight and average daily gain (ADG) in Batur lambs. The study sample consisted of 12 male and 8 female Batur lambs, aged 5 to 12 months, with an average body weight of 23.97 kg. Data were collected through a survey study using a random sampling method. The variables measured included chest girth, body length, sex, body weight, and ADG. The results demonstrated that: (1) the regression equation for chest girth on body weight was Y= -50.47 + 1.02CG (r = 0.91, R2 = 0.82); (2) the regression equation for body length on body weight was Y= -64.19 + 1.58BL (r = 0.88, R2 = 0.78); (3) the multiple regression equation for chest girth and body length on body weight was Y = -62.08 + 0.65CG + 0.69BL (r = 0.93, R2 = 0.85). These results indicate a very strong positive correlation between chest girth, body length, and body weight, suggesting that body weight in Batur lambs can be accurately predicted using these variables. However, ADG was not significantly correlated with chest girth or body length due to a low coefficient of determination
Polymorphisms of IGF-1 Gene in Indonesian Local Goat Reared Under Smallholder Farmers in Sulawesi Region
Full text linkABSTRACT. The objective of this study was to determine the allele frequency of IGF-1 in Kacang and Peranakan Ettawa goats in Indonesia. The DNA samples were extracted from the blood of 105 heads of goats and collected from the South Sulawesi and West Sulawesi provinces. The IGF-1 target was amplified using the PCR-RFLP method. Two allele variants (A and B) and three genotypes of the IGF-1 gene (AA, AB, and BB) have been identified in the local goat population. Allele frequencies of IGF-1 were A (0.81) and B (0.19) in the total population of local goats. Allele A is the most common allele in both the Kacang and Peranakan Ettawa goat populations, with the highest frequency found in the Kacang population (0.87). The most common genotype is genotype AA, with the highest frequency in the Kacang population (0.75). The observed and expected heterozygosity were 0.276 and 0.303, respectively. The population of local goats in these regions was in Hardy-Weinberg equilibrium. The conclusion of this research is that the IGF-1 gene in local goats in the Sulawesi region is polymorphic and this diversity information can be used for association studies with growth traits, litter size, and twinning rate.(Polimorpisme gen IGF-1 pada populasi kambing lokal Indonesia yang dipelihara oleh peternak kecil di daerah Sulawesi)ABSTRAK. Penelitian ini bertujuan untuk mengetahui frekuensi alel IGF-1 pada kambing Kacang dan Peranakan Ettawa di Indonesia. Sampel DNA diambil dari darah 105 ekor kambing dan dikumpulkan dari Provinsi Sulawesi Selatan dan Sulawesi Barat. Target IGF-1 diamplifikasi menggunakan metode PCR-RFLP. Dua varian alel (A dan B) dan tiga genotipe gen IGF-1 (AA, AB, dan BB) telah diidentifikasi pada populasi kambing lokal. Frekuensi alel IGF-1 adalah A (0,81) dan B (0,19) pada total populasi kambing lokal. Alel A merupakan alel yang paling banyak ditemukan baik pada populasi kambing Kacang maupun kambing Peranakan Ettawa, dengan frekuensi tertinggi terdapat pada populasi Kacang (0,87). Genotipe yang paling umum adalah genotipe AA, dengan frekuensi tertinggi pada populasi Kacang (0,75). Heterozigositas yang diamati dan diharapkan adalah 0,276 dan 0,303, masing-masing. Populasi kambing lokal di wilayah ini berada dalam keseimbangan Hardy-Weinberg. Kesimpulan yang dihasilkan adalah bahwa gen IGF-1 pada kambing lokal yang dipelihara oleh peternak di wilayah Sulawesi bersifat polimorfik dan informasi keragaman ini dapat digunakan untuk studi asosiasi dengan sifat pertumbuhan, litter size dan kelahiran kembar
Karakterisasi Dedak Padi dan Campuran Sekam Padi berdasarkan Sifat Fisik dan Kimia
Full text linkABSTRACT. Mengetahui karakteristik bahan berdasarkan sifat fisik dan kimia adalah sangat penting, agar dalam perancangan dan penarikan kesimpulan penelitian lebih akurat. Terutama penelitian dedak padi yang mempunyai kualitas yang beragam karena sangat ditentukan oleh metode dan mesin pada proses penggilingan gabah. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik dedak padi berdasarkan fraksi dan campurannya dengan sekam padi dengan berbagai metode evaluasi. Penelitian menggunakan dedak padi galur lokal unggul Banowati dari Desa Sindangsari, Sumedang. Penelitian tahap pertama untuk mengetahui karakteristik dedak padi berdasarkan fraksi ukuran partikel menggunakan saringan dengan mesh 10, 20, 30, 40 dan 50. Tahap kedua untuk mengetahui karakteristik campuran dedak padi dan sekam dengan proporsi 100, 75, 50, 25 dan 0 % dengan ukuran lolos mesh 30. Parameter yang diukur adalah Persentase ukuran partikel, gambaran visual, densitas, porositas, kandungan nutrien dan kandungan lignin. Hasil penelitian menunjukkan bahwa fraksi dedak padi berdasarkan ukuran partikel/mesh mempunyai karakteristik yang berbeda, perbedaan tersebut pada komposisi, kandungan nutrien, lignin, densitas dan porositas. Protein kasar mesh 30 sebesar 13,41%, mesh 40 sebesar 13,08%, dan mesh 50 sebesar 13,60. Uji lignin mesh 30, 40, dan 50 menunjukkan warna coklat terang. Berdasarkan sifat fisik dan kimia dedak padi lolos mesh 30, 40 dan 50 yang terbaik. Dedak padi yang dicampur dengan sekam padi yang semakin tinggi, maka semakin rendah nilai nutrien, kandungan lignin semakin tinggi, densitas dan porositas nya semakin tinggi.(Characterization of rice bran and rice husk mixture based on physical and chemical properties)ABSTRAK. Knowing the characteristics of materials based on their physical and chemical properties is very important for designing and drawing research conclusions more accurately. Especially rice bran research, which has a variety of quality because it is determined by the method and machine in the grain milling process. The purpose of this study was to determine the characteristics of rice bran based on its fraction and mixture with rice husks using various evaluation methods. The study used Banowati superior local strain rice bran from Sindangsari, Sumedang Village. The first stage of research was to determine the characteristics of rice bran based on particle size fractions using sieves with meshes of 10, 20, 30, 40, and 50. The second stage is to determine the characteristics of a mixture of rice bran and husk with proportions of 100, 75, 50, 25, and 0% with a mesh pass size of 30. The parameters measured are particle size percentage, visual image, density, porosity, nutrient content, and lignin content. The results showed that rice bran factions based on particle or mesh size have different characteristics, including differences in composition, nutrient content, lignin, density, and porosity. Crude protein mesh 30 is 13.41%, mesh 40 is 13.08%, and mesh 50 is 13.60. Lignin mesh assays 30, 40, and 50 show a light brown color. Based on the physical and chemical properties of rice bran, meshes 30, 40, and 50 are the best. The higher the rice bran mixed with rice husks, the lower the nutrient value, and the higher the lignin content, the higher the density and porosity
Kualitas Spermatozoa dan Tudung Akrosom Utuh pada Semen Beku Sapi Friesian Holstein dengan Mutu Genetik yang Berbeda
Full text linkABSTRAK. Penelitian ini bertujuan untuk mengetahui analisis kualitas spermatozoa dan tudung akrosom utuh semen beku pada pejantan sapi Friesian Holstein (FH) dengan mutu genetik yang berbeda. Metode penelitian yang digunakan adalah penelitian observasional dengan 2 kelompok perlakuan jenis semen beku yang berbeda. Kelompok perlakuan jenis semen beku adalah Sapi FH proven bull (P1) dan sapi FH grade B (P2). Parameter yang diamati meliputi motilitas spermatozoa, konsentrasi spermatozoa, viabilitas spermatozoa, abnormalitas spermatozoa, dan tudung akrosom utuh (TAU) spermatozoa. Data dianalisis menggunakan uji-t untuk mengetahui perbedaan kualitas antar kelompok perlakuan, kemudian dilakukan uji chi-square untuk membandingkan nilai hasil observasi dengan nilai harapan. Hasil penelitian menunjukkan bahwa kualitas semen beku pada sapi FH proven bull dan sapi FH grade B tidak berbeda nyata (P0,05). Disimpulkan bahwa perbedaan mutu genetik pada pejantan tidak memengaruhi kualitas spermatozoa semen beku sapi FH.(Sperm quality and intact acrosome cap of Friesian Holstein frozen semen with different genetic qualities)ABSTRACT. This study aimed to determine the quality of spermatozoa and intact acrosome cap of Friesian Holstein bulls with different genetic qualities. This study used observational research with two categories of frozen semen types treatment. The frozen semen consisted of Friesian Holstein Proven Bull (T1) and Friesian Holstein grade B bull (T2). The parameters observed include sperm motility, sperm concentration, sperm viability, sperm abnormality, and intact acrosome cap. Data were analyzed using a t-test to determine the differences in quality between treatments. Then, the chi-square test was used to compare the observed and expected values. The results showed no significant difference (P0.05) in frozen semen quality of Friesian Holstein proven bull and Friesian Holstein grade B bull. In conclusion, the different genetic qualities of bulls did not affect the quality of frozen semen
Stimulus Pertumbuhan Organ Reproduksi Ayam Buras Betina melalui Aplikasi Pemberian Phytoestrogen dari Kelompok Isoflavonoid Hasil Ekstrak Limbah Edamame
Full text linkABSTRAK. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan dan perkembangan organ reproduksi ayam buras betina yang diberi flavonoid dari ekstrak limbah kedelai edamame. Penelitian dilakukan dalam dua tahap; kuantifikasi total flavonoid ekstrak limbah kedelai edamame dan eksperimen menggunakan ayam buras berumur 8-16 minggu. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) terdiri atas empat kelompok umur dan lima perlakuan pemberian ekstrak limbah edamame per oral, dengan level 0% (P0), 0,02% (P1), 0,04% (P2), 0,08% (P3), dan 0,16% (P4) dari jumlah pakan yang diberikan. Variabel yang diamati yaitu pertambahan bobot badan (PBB), berat organ reproduksi, dan panjang saluran reproduksi. Data yang diperoleh dianalisis dengan analisis varian dan uji lanjut menggunakan Duncan. Hasil uji kuantitatif menunjukkan bahwa ekstrak limbah edamame mengandung senyawa flavonoid dari golongan isoflavon dan secara kuantitatif diperoleh 40 ppm total flavonoid. Penggunaan ekstrak limbah edamame secara signifikan meningkatkan PBB, panjang saluran reproduksi, dan berat organ (P0,05).(Stimulus for reproductive organs growth of female domestic chickens through phytoestrogens application of isoflavones group from edamame waste extracts)ABSTRACT. This study aimed to access the growth and development of female domesticated chicken reproductive organs after being treated with the addition of flavonoid compound from extract of edamae waste. The research consisted of two experiments; quantification of flavonoids compounds in edamame waste and feeding trial using female domesticated chickens (aged 4-16 weeks). The later used Randomized Block Design, consisted of four groups and five treatments (addition of edamame waste extract at 0%, 0.02%, 0.04%, 0.08%, and 0.16% of the amount of feed given). The variables observed were body weight gain, reproductive organ weight, and reproductive tract length. The data obtained were analyzed using analysis of variance analysis of variance (ANOVA) and Duncan's test. The phytochemical test showed that the edamame waste extract contained flavonoid compounds from the isoflavone group. The addition of edamame waste extract significantly affected body weight gain, reproductive tract length, and reproductive organ weight (P0.05)
Pertumbuhan dan Produksi Indigofera zollingeriana pada Lahan Pasca Tambang Pasir dengan Penambahan Pupuk dan Mikoriza
Full text linkABSTRAK. Penelitian ini bertujuan untuk mengetahui pertumbuhan dan produksi legum Indigofera zollingeriana pada lahan pasca tambang pasir dengan penambahan pupuk dan mikoriza. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan lima ulangan. Perlakuan yang diberikan adalah pupuk organik berupa pupuk kandang kambing, pupuk anorganik berupa NPK, FMA (Fungi Mikoriza Arbuskula), MPF (Mikroba Pelarut Fosfat) dan pupuk hayati berupa Asam humat. Hasil penelitian memperlihatkan bahwa perlakuan P1 sampai P6 pada pertumbuhan legum Indigofera zollingeriana dengan pemberian pupuk organik, anorganik dan hayati tidak memberikan pengaruh nyata terhadap tinggi tanaman, diameter batang dan jumlah tangkai Indigofera zollingeriana pada pemotongan I dan II. Perlakuan P2 sampai P6 pada produksi legum Indigofera zollingeriana memperlihatkan pengaruh yang sangat nyata (P0,01) terhadap produksi tanaman pada berat kering daun dan berat kering ranting pemotongan I. Perlakuan P1 sampai P6 pada berat kering batang pemotongan I, berat kering daun pemotongan ke II, berat kering batang pemotongan ke II, berat kering ranting pemotongan ke II, produksi biomassa dan berat kering akar tidak memperlihatkan pengaruh nyata. Kesimpulan dari penelitian adalah Pemberian pupuk organik, dan FMA tidak berpengaruh terhadap pertumbuhan, namun penambahan pupuk NPK dapat meningkatkan pertumbuhan dan produktivitas tanaman Indigofera zollingeriana pada lahan pasca tambang pasir.(Growth and production of Indigofera zollingeriana in post-sand mining land with addition Fertilizer and mycorrhizae)ABSTRACT. This research aims to determine the growth and production of Indigofera zollingeriana legumes on post-sand mining land with the addition of fertilizer and mycorrhiza. The experimental design used was a Completely Randomized Design (CRD) with six treatments and five replications. The treatment given is organic fertilizer in the form of goat drum fertilizer, inorganic fertilizer in the form of NPK, AMF (Arbuscular Mycorrhizal Fungi), MPF (Phosphate Solvent Microbes) and biological fertilizer in the form of humic acid. The results of the research showed that treatment P1 to P6 on the growth of Indigofera zollingeriana legumes by providing organic, inorganic and biological fertilizers did not have a significant effect on plant height, stem diameter and number of Indigofera zollingeriana stalks at cuttings I and II. Treatments P2 to P6 on the production of Indigofera zollingeriana legumes showed a very significant effect on (P0.01) plant production on dry weight of leaves and dry weight of twigs cut I. Treatments P1 to P6 on dry weight of stems cut I, dry weight of leaves cut II, dry weight of second cutting stems, dry weight of second cutting twigs, biomass production and root dry weight did not show a significant effect. The conclusion of the research is that the provision of organic fertilizer and AMF has no effect on growth, but the addition of NPK fertilizer can increase the growth and productivity of Indigofera zollingeriana plants on post-sand mining land
The Impact of Turmeric, Curcuma, and Ginger Feed Additives on the Productivity of Kaur Cattle as a Genetic Resource in Bengkulu
Full text linkKaur cattle, a local breed in Bengkulu, represent a valuable genetic resource that requires preservation and effective utilization to enhance livestock production. Turmeric, curcuma, and ginger are plants known for their essential oils and curcumin content, which offer numerous proven benefits. This study aimed to evaluate the impact of these feed additives on the productivity of Kaur cattle. A quantitative research method employing a completely randomized design was used, consisting of four treatments and four replications. The diet comprised wild grass (10% of body weight) and palm kernel meal in an 80:20 ratio. The treatments were as follows: P0 (control: wild grass and palm kernel meal), P1 (wild grass, palm kernel meal, and turmeric powder Curcuma xanthorrhiza Roxb.), P2 (wild grass, palm kernel meal, and curcuma powder Curcuma longa L.), and P3 (wild grass, palm kernel meal, and ginger powder Zingiber officinale Rosc.). The study involved 16 male Kaur cattle aged 1.5-2 years, with an average body weight of 160-180 kg, observed over 60 days. The variables measured included average daily weight gain, forage consumption, total feed consumption, feed efficiency, and cost efficiency. Data were analyzed using a one-way ANOVA. The results showed that the mean daily weight gain (kg/head/day) was P0=0.352.57, P1=0.472.39, P2=0.502.46, and P3=0.492.88, with no significant differences between treatments. The mean feed efficiency (%) was P0=0.124.44, P1=0.254.71, P2=0.275.22, and P3=0.264.38, also showing no significant differences. The mean cost efficiency for feed use was P0=35,8697.47, P1=34,2817.08, P2=33,2207.39, and P3=35,4107.21, with no significant differences observed. Overall, the addition of turmeric, curcuma, and ginger powders to the feed did not significantly impact the productivity of Kaur cattle