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Pertumbuhan, Produksi dan Kandungan Nutrisi Hijauan Unggul pada Tingkat Naungan yang Berbeda
Get PDFABSTRAK. Penelitian bertujuan untuk mendapatkan spesies hijauan pakan unggul yang toleran terhadap naungan agar dapat dikembangkan pada lahan perkebunan kelapa sawit. Penelitian dilaksanakan di kebun percobaan Gowa Balai Pengkajian Teknologi Pertanian (BPTP) Balitbangtan Sulawesi Selatan. Penelitian menggunakan 5 spesies hijauan unggul yaitu Brachiaria hybrid cv. Mulato, Paspalum atratum, Setaria sphacelata, Brachiaria brizantha, dan Panicum maximum pada tiga tingkat naungan yaitu 0%, 50% dan 65%. Setiap spesies hijauan ditanam di bawah naungan berukuran 2 x 1,5 m2 dengan tiga ulangan dalam rancangan petak terbagi. Peubah yang diukur yaitu laju pertumbuhan relatif, luas daun spesifik, laju asimilasi bersih, produksi kumulatif, dan kandungan nutrisi (protein, NDF dan ADF). Hasil yang diperoleh menunjukkan bahwa Brachiaria hybrid cv. Mulato dan Panicum maximum memiliki laju pertumbuhan relatif yang lebih tinggi pada berbagai tingkat naungan dan nilai laju asimilasi bersih tinggi meskipun ternaungi hingga 65%, namun nilai rata-rata luas daun spesifik (LDS) paling rendah dibanding hijauan lainnya. Simpulan spesies Brachiaria hybrid cv. Mulato merupakan hijauan unggul yang paling toleran terhadap naungan dibanding keempat spesies hijauan lainnya.(Growth, production, and content of superior forage nutrients at different shade levels)ABSTRACT The research aimed to obtain superior` forage species that are tolerant to shade so they can be developed on oil palm plantations. The research was carried out in the Gowa experimental garden of the Balai Pengkajian Teknologi Pertanian (BPPT) South Sulawesi. This study used 5 superior forage species namely Brachiaria hybrid cv. Mulato, Paspalum atratum, Setaria sphacelata, Brachiaria brizantha, Panicum maximum and three shading levels of 0%, 50% and 65%. Each forage species is planted in a 2 x 1.5 m2 shade with three replications in a divided plot design. The variables measured are relative growth rates, specific leaf area, net assimilation rate, cumulative production, and nutrient content (protein, NDF and ADF). The results obtained showed that Brachiaria hybrid cv. Mulato and Panicum maximum have a higher relative growth rate at various shade levels and the value of the net assimilation rate is high even though it is shaded by 65%, but the average specific leaf area value is the lowest compared to other forages. The conclusion is Brachiaria hybrid species. Mulato is a superior forage that is most tolerant to shade compared to the other four forage species
Isolasi Candida sp. dan Aspergilus sp. pada Tembolok (Ingluviens) Ayam Ras dan Ayam Buras di Pasar Peunayong, Banda Aceh
Get PDFABSTRAK. Penelitian ini bertujuan mengisolasi Candida sp. dan Aspergillus sp. pada tembolok ayam ras dan buras. Sampel dalam penelitian ini adalah tembolok ayam ras dan buras masing-masing berjumlah 15 sampel yang diambil secara acak dari tempat pemotongan unggas Peunayong Banda Aceh. Isolasi Candida sp. dan Aspergillus sp. dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang diberi antibiotik selanjutnya ditanamkan pada media Sabourauds Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pengamatan morfologi Candida sp. dan Aspergillus sp. diamati secara makroskopis. Koloni yang diduga Candida sp. dan Aspergillus sp. diperiksa secara mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Candida sp. dapat diisolasi pada semua sampel (100%) tembolok ayam ras dan ayam buras. Aspergillus sp. dapat diisolasi pada 2 dari 15 (13,33%) sampel tembolok ayam ras dan 6 dari 15 (40%) sampel tembolok ayam buras. Kesimpulan penelitian ini adalah Candida sp. tidak ada perbedaan pada tembolok ayam ras dan ayam buras sedangkan Aspergillus sp. pada tembolok ayam buras lebih banyak dari pada ayam ras.(Isolation of Candida sp and Aspergillus sp. from crops (Ingluviens) of broiler and indigenous chicken in Peunayoung market, Banda Aceh)ABSTRACT. This research aimed to isolate Candida sp. and Aspergillus sp. from crop of chicken race (broiler) and indigenous chicken. This research used crops of the chicken race (broiler) and indigenous chicken, each animal consists of 15 animals taken randomly from the poultry of slaughter house Peunayong Banda Aceh. Isolation of Aspergillus sp. was done based on Thompson method (1969). The samples were washed with sterile aquadest containing antibiotics before implanted on Sabourauds Dextrose Agar (SDA), then incubated at room temperature for 2-7 days. The plate was observed from Candida sp. and Aspergillus sp. colony macroscopically and microscopically. Data were analyzed descriptively. The result showed that Candida sp. Found in all samples (100%) and Aspergillus sp. Found in 2 out of 15 (13,33%) crops samples in chicken race (broiler) and 6 out of 15 (40%) crops in indigenous chicken. The conclusion is, candida was found in both chickens race (broiler) and domestic chicken, while aspergillus was found more in indigenous chicken than chickens race broiler
Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST
Get PDFABSTRAK. Penelitian ini bertujuan untuk mengetahui waktu ekuilibrasi yang terbaik semen kerbau menggunakan pengencer andromed dan mengetahui nilai membran plasma utuh semen kerbau dalam larutan hipoosmotik pada waktu pemeraman yang berbeda. Penelitian ini terdiri dari dua tahap; Tahap pertama menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perlakuan terdiri dari waktu ekuilibrasi 3 jam, 4 jam dan 5 jam. Parameter yang diukur adalah motilitas, sperma hidup dan abnormalitas. Penelitian tahap kedua menggunakan Rancangan Acak Lengkap (RAL) dua faktor dengan 3 perlakuan dan 5 ulangan. Faktor pertama adalah waktu ekuilibrasi yang terdiri dari 3 jam, 4 jam dan 5 jam. Faktor kedua adalah waktu pencampuran semen dengan larutan hipoosmotik yang terdiri 15 menit, 30 menit dan 45 menit. pengencer yang digunakan Andromed(R) (Minitue Germany, 13503/0200). Parameter yang diukur adalah persentase membran plasma utuh semen kerbau. Hasil penelitian yang didapatkan bahwa waktu ekuilibrasi terbaik adalah 4 jam dan 5 jam dengan nilai motilitas 62,8-63,7%, sperma hidup 66,7-66,8% dan persentase abnormalitas 10,5-11,2%. Waktu HOS test yang terbaik adalah 45 menit dan waktu ekuilibrasi 5 jam dengan nilai MPU (64,7 : 57,7%). Kesimpulan penelitian ini adalah waktu ekuilibrasi semen kerbau menggunakan pengencer andromed adalah 5 jam dengan waktu pemeraman dalam larutan hipoosmotik adalah 45 menit.(Semen quality of buffalo at different equilibration and incubation period in hipoosmotic solution of swelling TEST)ABSTRACT The aimed of this study was to find out the best equilibration time of buffalo semen used andromed diluents and the optimal time to test the integrity of plasma membrane of buffalo semen using a hypoosmotic swelling test (HOS test). This study consists of two stages; the first step was used a Completely Randomized Design (CRD) with 3 treatments and 5 replications. The treatments were equilibration time of 3 hours, 4 hours and 5 hours. The parameters measured were motility, live sperm, and abnormalities. The second stage of the study used a two-factor Completely Randomized Design (CRD) with 3 treatments and 5 replications. The first factor was the equilibration time of 3 hours, 4 hours and 5 hours. The second factor was the time of semen mixing with a hypoosmotic solution of 15 minutes, 30 minutes and 45 minutes. diluents used by Andromed(R) (Minitube Germany, 13503/0200). The parameters measured were the percentage of the integrity of the plasma membrane of buffalo semen. The results showed that the best equilibration time was 4 hours and 5 hours with motility values 62.8-63.7%, live sperm 66.7-66.8% and percentage abnormalities 10.5-11.2%. The best HOS test time is 45 minutes and the equilibration time is 5 hours with MPU value (64.7: 57.7%). The conclusion of the research is that the equilibration time of buffalo semen using andromed diluents was 5 hours with the mixing time in the hypoosmotic solution being 45 minutes
Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat
Get PDFABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.(The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck)ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status
Karakteristik Fisiko Kimia Tepung Ikan yang Diberi Pengawet Bawang Putih (Allium sativum) pada Masa Penyimpanan yang Berbeda
Get PDFABSTRAK. Penelitian ini bertujuan untuk mengetahui karakteristik fisiko kimia tepung ikan yang diberi tepung bawang putih (Allium sativum) pada masa penyimpanan yang berbeda. Rancangan penelitianyang digunakan adalah Rancangan Acak Lengkap Pola Faktorial (RALF) dengan faktor A adalah tepung ikan dengan 3 level penambahan tepung bawang putih (0, 1, 2 dan 3%) dan Faktor B adalah lama penyimpanan tepung ikan (1, 2, 3 dan 4 minggu). Setiap perlakuan diulang sebanyak 3 kali. Variabel yang diamati dalam penelitian ini adalah Warna, Aroma, Kadar Air (KA), Kadar Abu (KA), Lemak Kasar (LK) dan Protein Kasar (PK). Hasil penelitian menunjukkan bahwa penambahan tepung bawang putih di dalam tepung ikan dengan level 0, 1, 2 dan 3% dengan lama penyimpanan yang berbeda berpengaruh nyata terhadap meningkatkan kadar air, tetapi menurunkan abu dan protein kasar. Interaksi antara level tepung bawang putih yang ditambahkan pada tepung ikan dan lama penyimpanan yang berbeda berpengaruh nyata (P0,05) terhadap kadar lemak kasar dan protein kasar.(Physicochemical characteristics of fish meal with garlic (Allium sativum) preservatives at different storage periods)ABSTRACT. This study aimed to determine the physicochemical characteristics of fish meal fed with garlic flour (Allium sativum) at different storage periods. The research design used was a Factorial Complete Randomized Design (RALF) with factor A being fish flour with 3 levels of adding garlic flour (0, 1, 2 and 3%) and Factor B was the storage time of fish meal (1, 2, 3 and 4 weeks). Each treatment was repeated 3 times. The variables observed in this study were colour, aroma, water content (KA), ash content (KA), crude fat (LK) and crude protein (PK). The results showed that the addition of garlic flour in fish meal with levels 0, 1, 2 and 3% with different storage periods significantly affected water content, but reduced ash and crude protein. The interaction between the level of garlic flour added to fish meal and the different storage times significantly (P0.05) on crude fat and crude protein
Evaluasi Kecernaan In Vitro Bahan Pakan Hasil Samping Agro Industri
Get PDFABSTRAK. Penelitian ini bertujuan untuk mengevaluasi nilai kecernaan bahan kering, kecernaan bahan organik dan nilai TDN bahan pakan hasil samping agro industry. Evaluasi dilakukan secara in vitro menggunakan cairan rumen kambing Peranakan Ettawa dengan pakan PK 12% dan TDN 62%. Penelitian menggunakan metode eksperimental rancangan acak lengkap 7 perlakuan dan 3 ulangan. Pakan perlakuan terdiri atas ampas tahu, bungkil kopra, bungkil kelapa sawit, bungkil kedelai, onggok, kulit kopi, dan janggel jagung. Data dianalisis menggunakan ANOVA dan n uji lanjut Duncan. Hasil penelitian menunjukkan adanya perbedaan kecernaan bahan kering, kecernaan bahan organik dan nilai TDN (P0,05) antara bahan pakan hasil samping agro industri. Ampas tahu memiliki kecernaan bahan kering (73,03%), kecernaan bahan organik (71,66%), dan nilai TDN (71,88%) lebih tinggi dibanding bungkil kopra, bungkil kelapa sawit, bungkil kedelai, onggok, janggel jagung dan kulit kopi.(In vitro digestibility evaluation of feed ingredients from agro-industry by-product)ABSTRACT. This study aimed to evaluate the value of dry matter-, organic matter digestibility and TDN of feed ingredients from agro-industrial by product. In vitro evaluation using rumen fluid of Ettawa crossbreed goat feeding with 12% PK and 62% TDN. The experimental used a completely randomized design with 7 treatments and 3 replications. The feed treatments were tofu waste, coconut meal, palm kernel meal, soybean meal, onggok, coffee husk, and corn cob. Data were analyzed using ANOVA and continued by Duncan test. The results showed differences in dry matter digestibility, organic matter digestibility and TDN value (P 0.05) between feed ingredients from agro-industrial by product. Tofu waste has dry matter digestibility (73.03%), organic matter digestibility (71.66%), and TDN value (71.88%) higher than coconut meal, palm kernel meal, soybean meal, onggok, corn cob and coffee husk
Perbandingan Model Pendugaan Total Digestible Nutrients (TDN) dan Protein Tercerna pada Domba Garut Jantan yang Diberi Ransum Berbasis Bahan Pakan Lokal
Get PDFABSTRAK. Perhitungan total digestible nutrients (TDN) dan Protein tercerna secara biologis seringkali mengalami kesulitan sehingga dilakukan perhitungan dengan menggunakan model pendugaan. Penelitian bertujuan untuk membandingkan model pendugaan TDN dan protein tercerna pada domba Garut jantan yang diberi ransum berbahan baku pakan lokal. Dua puluh empat ekor domba Garut diberi ransum berbasis bahan pakan lokal dengan kandungan TDN dan protein berbeda, lalu diukur nilai TDN dan protein tercerna. Nilai keakuratan model pendugaan TDN dan protein tercerna diukur dengan perhitungan ratio prediction to deviation (RPD), Hubungan TDN dan protein tercerna in vivo dengan berbagai model pendugaan dilakukan dengan menggunakan analisis regresi. Model pendugaan yang digunakan untuk mengukur TDN adalah model Sutardi, Wardeh dan Harris et al., sedangkan model pendugaan protein tercerna menggunakan model Beenson dan Knight dan Haris. Hasil menunjukkan bahwa model pendugaan TDN Wardeh lebih akurat dibandingan dengan model Sutardi maupun Beenson dengan nilai ratio prediction to deviation (RDP) = 2,45, R2 = 08629 dan r = 0,9289. Model pendugaan protein tercerna Beenson dan Knight dan Haris tidak dapat digunakan karena memiliki nilai RDP yang sangat rendah. Kesimpulannya model pendugaan Wardeh lebih akurat dalam mengukur TDN pada domba Garut jantan.(Comparison of the total digestible nutrients (TDN) and digestible proteins models in male Garut sheep fed local feed-based rations)ABSTRACT. Calculation of total digestible nutrients (TDN) and digested proteins biologically are often difficult, so calculations are made using the estimation model. The study aimed to compare the estimation model of TDN and digestible proteins in male Garut sheep fed local feed-based rations. Twenty-four of male Garut sheep were given various types of rations based on local feed ingredients with different TDN and protein content, then measured the value of TDN and digested protein. Then the accuracy of the TDN and digested protein estimation model was measured by calculating the ratio of prediction to deviation (RPD), while measuring the relationship of TDN and digested proteins In Vivo with various estimation models was carried out using regression analysis. The estimation model used to measure TDN was the Sutardi, Wardeh and Harris et al. models, while the digested protein estimation model is using Beenson and Knight and Haris models. The results show that the Wardeh TDN estimation model is more accurate compared to the Sutardi and Beenson models with the RDP = 2.45, R2 = 0.8629 and r = 0.9289. Beenson and Knight and Haris digestible protein estimation model cannot be used because it has a very low RDP value. The conclusion is Wardeh estimation model is more accurate in measuring TDN in male Garut sheep
Evaluasi Kinerja Pertumbuhan Sapi Silangan Belgian Blue dan Peranakan Ongole
Get PDFABSTRAK. Tujuan penelitian ini adalah mengevaluasi kinerja pertumbuhan sapi persilangan Belgian Blue (BB) dan sapi Peranakan Ongole (PO) pada turunan pertama (F1). Sapi persilangan (BB dan PO) yang digunakan sebanyak 15 ekor terdiri atas jantan 6 ekor dan betina 9 ekor, selain itu digunakan 8 ekor sapi PO betina yang dipelihara di Balai Embrio Ternak (BET) Cipelang Bogor. Peubah yang diamati adalah bobot lahir (BW), bobot sapih (WW) 205 hari, bobot 1 tahun (YW), pertambahan bobot badan harian dari lahir (GBW) sampai sapih (GWW) dan pertambahan bobot badan harian dari sapih (GWW) sampai umur 1 tahun (GYW). Data dianalisis secara deskriptif dan perbedaan antar sapi silangan (BB-PO) dengan sapi PO dilakukan uji t. Hasil analisis bobot badan dan pertumbuhan menunjukkan bahwa, bobot badan sapi silangan BB dan PO lebih tinggi dibandingkan dengan sapi PO pada WW 205 hari (165,221,3 kg dan 11515,2 kg), YW (365 hari) (240,728,1 kg dan 194,926,1 kg) dan GBW sampai GWW (0,6750,097 kg dan 0,4410,059 kg). BW dan GWW sampai GYW tidak berbeda antara sapi silangan BB-PO dengan PO. Hasil penelitian ini menunjukkan bahwa program cross-breeding sapi BB dengan PO dapat meningkatkan kinerja bobot badan dan pertumbuhan.(Growth performance evaluation of belgian blue and ongole crossbreed)ABSTRACT. The purpose of this study was to evaluate the growth performance of cross-breeding of Belgian Blue (BB) and Ongole Grade (PO) cattle in the first generation (F1). Crossbred of BB and PO cattle used as many as 15 individuals consisting of 6 males and 9 females, also, 8 individual PO females are used which are kept in the Livestock Embryo Center (BET) Cipelang, Bogor. The observed variables were birth weight (BW), weaning weight (WW) 205 days, 1-year weight (YW) 365 days, daily body weight gain from birth (GBW) to weaning (GWW) and daily body weight gain from weaning (GWW) until the age of 1 year (GYW). Data were analyzed descriptively and the difference between cross-breeding (BB-PO) cattle and PO cattle was analyzed by t-test. The results of body weight and growth analysis showed that crossbred BB and PO cattle were higher than PO cattle at WW 205 days (165.2 21.3 kg and 115 15.2 kg), YW (365 days) (240.7 28.1 kg and 194.9 26.1 kg ) and GBW to GWW (0.675 0.097 kg and 0.441 0.059 kg). BW and GWW to GYW do not differ between crossbred BB-PO and PO cattle. The results of this study indicate that the cross-breeding program for BB cattle with PO cattle breeds can improve the performance of body weight and growth
Evaluasi Program Inseminasi Buatan pada Sapi Lokal Betina di Kecamatan Juli, Kabupaten Bireuen, Provinsi Aceh
Get PDFABSTRAK. Penelitian ini bertujuan untuk mengetahui keberhasilan program inseminasi buatan pada sapi lokal betina. Penelitian ini dilakukan di Kecamatan Juli, Kabupaten Bireuen, Provinsi Aceh, yaitu Desa Keude Dua, Bunyot, Blang Ketumba, Paya Cut dan Desa Batee Raya. Metode survei digunakan dalam penelitian ini. Responden yang digunakan adalah peternak sapi lokal dan inseminator. Responden ditetapkan dengan menggunakan metode purposive sampling. Sebagai responden ditetapkan 40 peternak dan 4 inseminator. Peternak responden harus memenuhi kriteria memelihara minimal dua ekor sapi lokal yang telah beranak 2 (dua) kali dan sistem perkawinan dilakukan secara inseminasi buatan (IB). Inseminator telah memiliki Surat Izin melakukan Inseminasi Buatan (SIMI). Hasil penelitian menunjukkan bahwa pelaksanaan program inseminasi buatan di Kecamatan Juli telah berjalan dengan baik, dimana rataan nilai conception rate (CR) sebesar 88,05%, service per conception (S/C) adalah 1,2, jarak beranak (calving interval) sebesar 12,36 bulan, dan calving rate sebesar 84,23%.(Evaluation of artificial insemination programs in local cows in Juli district, Bireuen, Aceh Province)ABSTRACT. This study aimed to determine the success of the artificial insemination program in local female cattle.This research was carried out in Juli District, Bireuen , Aceh Province, namely the villages of Keude Dua, Bunyot, Blang Ketumba, Paya Cut and Batee Raya villages. The survey method was used in this study. Respondents were determined by purposive sampling method. Respondents used were local cattle breeders and inseminators. The respondents involved ware 40 breeders and 4 inseminators. Breeders must maintaining minimal two female local cows that have given birth 2 (two) times with artificial insemination as mate system. The Inseminator involved is the one who has Artificial Insemination License. The results showed that the implementation of the artificial insemination program in the District of Juli has gone well, where the average value of the conception rate (CR) is 88.05%, service per conception (S/C) is 1.2, calving interval equal to 12.36 months and calving rate is 84.23%
Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3)
Get PDFProteases are one of most important and abundant enzymes produced by the biotechnology industry, for scientific, physiological and industrial application and dominates of the whole enzyme market. Lactobacillus plantarum IIA-1A5 is an Indonesian lactic acid bacteria (LAB) isolated from beef Peranakan Ongole cattle. Preliminary analysis on its whole genome sequence indicated that this strain harbours some genes involved in protein degradation and might be promising to be further applied. This study aims to optimize the gene sequence of a lon-like protease of L. plantarum IIA-1A5 for heterologous expression system. The Lon-like gene expression system is made using genes that have been optimized first in silico. pET-28a(+), E. coli BL21(DE3), Nde1 and BamH1 were used in this study as a expression vector, a host and retriction enzyme, respectively. Molecular weight was validated using SDS-PAGE and expasy.org software. The results showed that optimization increased codon adaptation index value (CAI) and GC content to 0.97 and 56.57%, respectively, which were suitable for the E. coli expression system. The Lon-like IIA gene was successfully expressed in the cell cytoplasm by induction of 1 mM Isopropyl -D-1-thiogalactopyranoside (IPTG) at 37 C. As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. Lon-like IIA was successfully expressed in a host cell induced with 1 mM IPTG at 37oC . IPTG induction was performed at the 3rd hour of incubation with OD600 0.59. In addition, Lon-like IIA molecular weight was detected approximately 43 kDa