Hochschule Bonn-Rhein-Sieg

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    7939 research outputs found

    CE-Kennzeichnung

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    Dieses Video (ca. 2,5 Minuten) aus der Videoreihe „Normen-ABC“ erklärt den Ablauf einer CE-Kennzeichnung in Zusammenhang mit den zu erfüllenden EU-Richtlinien. Es zeigt den Unterschied zum sehr ähnlichen Zeichen für „China Export“

    Digitale Lernangebote schaffen – von der Teaching Library zum E-Learning-Zentrum

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    Das Potenzial digitaler Informations- und Schulungsangebote, deren Ausgestaltung zum klassischen Aufgabenportfolio der Teaching Library gehört, hat sich im Zuge der Digitalisierung sowohl technisch als auch didaktisch stark ausdifferenziert. Darüber hinaus übernehmen Bibliotheken die Entwicklung und Betreuung zentraler Service-Strukturen für das digitale Lehren und Lernen an Hochschulen und entwickeln sich so zu E-Learning-Zentren weiter. Welche Chancen diese strategische Neuausrichtung für die Positionierung von Bibliotheken als Ganzes hat, wie sich dies auf die Gestaltung bereits bestehender Schulungsangebote auswirken kann und welcher sich wandelnde Kompetenzbedarf daraus hervorgeht, wird im Folgenden anhand eines praktischen Lehrbeispiels zur Vermittlung wissenschaftlicher Schreibkompetenz aufgezeigt

    Pitfalls of using sequence databases for heterologous expression studies - a technical review

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    Synthesis of DNA fragments based on gene sequences available in public resources has become an efficient and affordable method that gradually replaced traditional cloning efforts such as PCR cloning from cDNA. However, database entries based on genome sequencing results are prone to errors which can lead to false sequence information and, ultimately, errors in functional characterization of proteins such as ion channels and transporters in heterologous expression systems. We have identified five common problems that repeatedly appear in public resources: 1) Not every gene has yet been annotated; 2) Not all gene annotations are necessarily correct; 3) Transcripts may contain automated corrections; 4) There are mismatches between gene, mRNA, and protein sequences; and 5) Splicing patterns often lack experimental validation. This technical review highlights and provides a strategy to bypass these issues in order to avoid critical mistakes that could impact future studies of any gene/protein of interest in heterologous expression systems. Abstract figure legend Projects involving heterologous gene expression are often characterised by similar steps. Initially, database research (A) is necessary to retrieve information of full of partial sequences of a gene of interest. A multitude of genome assemblies are annotated and deposited in public databases or that are available for refined search options using individual sequence information. The search results need to be scrutinised and compared to already available information (B). Once the sequence has been determined, DNA synthesis (C) by PCR or commercial synthesis are necessary for further cloning procedures (D). Eventually, the DNA needs to be transfected (E) and expressed in, e.g., eukaryotic cells (F). Finally, the expression of the gene of interest needs to be documented and its function analysed (G). This article is protected by copyright. All rights reserved

    Comunity of NKS raw data

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    Full data on citizen questionnaire, conducted in 2018. as an online survey, with the sample size of 808 respondents. In order to qualify for participation in the survey, the respondents needed to be citizens of the rural community Neunkirchen-Seelscheid with voting rights, meaning older than 16 years

    IEEE Joint IES/IAS/PELS German Chapter Organizes Student Summit

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    Becoming an Online Wine Taster: An Ethnographic Study on the Digital Mediation of Taste

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    There has been a growing interest in taste research in the HCI and CSCW communities. However, the focus is more on stimulating the senses, while the socio-cultural aspects have received less attention. However, individual taste perception is mediated through social interaction and collective negotiation and is not only dependent on physical stimulation. Therefore, we study the digital mediation of taste by drawing on ethnographic research of four online wine tastings and one self-organized event. Hence, we investigated the materials, associated meanings, competences, procedures, and engagements that shaped the performative character of tasting practices. We illustrate how the tastings are built around the taste-making process and how online contexts differ in providing a more diverse and distributed environment. We then explore the implications of our findings for the further mediation of taste as a social and democratized phenomenon through online interaction

    Twitchen als Kulturtechnik

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