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NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS
Get PDFBackground: Chronic Periodontitis (CP) and Aggressive Periodontitis (AP) are chronic inflammation diseases in the dental supporting tissues characterized by the existence of pocket inside, alveolar bone attachments and damages rapidly leading to the dental loss. The pocket inside is related to the existence of the dominating bacteria known as the black-pigmented anaerobic bacteria group. This black-pigmented bacteria group consists of Porphyromonas spp and Prevotella spp genus abundantly found in the periodontal pocket. This research aims at examining the number of black-pigmented bacteria taken from the Gingival Crevicular Fluid (GCF) of patients suffering from the Chronic Periodontitis (CP) and Aggressive Periodontitis (AP).Method: The ethical clearance was obtained from the Faculty of Dentistry, Universitas Jember. The subjects diagnosed with the Chronic Periodontitis (CP) and Aggressive Periodontitis were recorded into pocket and then the panoramic Rontgen photographs were taken. The Gingival Crevicular Fluid (GCF) of patients were taken using a sterile paper point and then inserted to the pocket inside for 30 seconds. The paper point was then inserted to the PBS and examined using a microbiological test on blood agar media. The mouth cavity hygiene levels of subjects were recorded using OHI-S, while the needs on periodontal treatments using CPITN. The statistical test was conducted using T-test with the value of p0.05). this shows the same periodontal disease severity of both Chronic Periodontitis (CP) and Aggressive Periodontitis (AP). The bacteria culture result shows that the number of black- pigmented anaerobic bacteria in both Chronic Periodontitis (CP) and Aggressive Periodontitis (AP) was not significant (p>0.05). The mouth hygiene level of patients suffering from the Chronic Periodontitis (CP) and Aggressive Periodontitis (AP) was at the medium (93%) level with the needs on periodontal treatments of scaling and root planning (85%)Conclusion: The number of black-pigmented anaerobic bacteria colonies belonging to the patients suffering from the Chronic Periodontitis (CP) and Aggressive Periodontitis (AP) was same
CHARACTERISTIC OF ORAL MEDICINE PATIENT USING TELEDENTISTRY DURING COVID-19 PANDEMIC
Get PDFBackground: The COVID-19 pandemic has affected health care systems around the world. Dentistry is one of the most closely related to the production of aerosols or droplets. Minimal visits to a dental clinic or hospital are highly recommended except in emergency cases. The government's recommendation is the use of teledentistry to conduct diagnosis, treatment, and monitoring of diseases. The oral medicine field is fully in charge of this activity and until now it has never been studied how the characteristics of oral medicine patients who use teledentistry services including their level of satisfaction. Method: To describe the characteristic including the level of satisfaction of Oral Medicine patients using teledentistry in Dental Hospital Usakti during pandemic COVID-19. An observational cross-sectional study was conducted in the Oral Medicine Clinic in 31 patients who used teledentistry services at the beginning of the COVID-19 pandemic under informed consent. Validated modified questionnaire and sociodemographic data were collected and satisfaction scores were categorized. Results: The validity of the questionnaire showed Cronbach-alpha 0.83 with a reliability of 0.95 and a separation of 4.49. Most of the subject used teledentistry was female (54.83%), 31-60 years old (51.61%), no history of contact with COVID-19 patient, and prior history of teledentistry (100%, 77.41%, respectively). 93.6% of subjects showed a high satisfaction level in using teledentistry services. Conclusion: The characteristic of oral medicine patients using teledentistry described a high satisfaction level, thus the teledentistry method could be a solution in/after the pandemic era
THE EFFECT OF SOURSOP (ANNONA MURICATA L.) ESSENTIAL OILS ON VIABILITY CELLS: AN IN-VITRO STUDY
Get PDFBackground: Development of new preventive agents for dental caries is needed. One of the candidates for preventive agents from natural products is Soursop leaf. The present study aimed to determine the effect of Soursop leaf oil on the cultured epithelial and fibroblast cells.Methods: In this experimental study, Soursop leaf essential oils were provided, and their effect was discovered on epithelial and fibroblast cells line using MTT assay. The MTT assay was conducted to measure the activity of enzymes that reduce MTT and switch it to formazan dye creating a purple colour. Using a microplate reader, the optical density was measured at 550 nm and the absorbance value directly represented relative cell numbers.Results: Data compilation and analysis were done using one-way analysis of variance. Soursop leaf essential oils exhibited variable noxious effects on cultured cells. The present study shows that epithelial cell death was less than 30% at the concentration 2.5 µl/ml while the percentage of fibroblast cell death was less than 30% at smaller concentrations of 1.25 µl/ml. Through an increase in the concentration of Soursop leaf essential oils, the toxicity of these materials substantially increased (p<0.05)Conclusion: Soursop leaf essential oils at certain concentrations may cause epithelial and fibroblast cell death
PROPERTIES OF COMPOSITE RESIN ALKASIT AND ZIRCONIA-REINFORCED GLASS-IONOMER CEMENT IN DIFFERENT STORAGE
Get PDFBackground: The temperature and salivary pH in a person's mouth are highly dynamic (e.g., before, during, and after eating) and so restorations in a cavity must be resilient to these variable conditions. Temperature and immersion conditions affect the mechanical properties of a restoration. This study aimed to determine the effect of environmental conditions on diametral tensile strength (DTS) and surface microhardness of a resin composite with alkaline fillers or zirconia–reinforced glass ionomer cement (Zr-reinforced GIC). Method: Thirty specimens of a resin composite with alkaline fillers (Cention-N, Ivoclar-Vivadent, Lichtenstein) and 30 specimens with zirconia-reinforced GIC (Zirconomer, Shofu, Japan) were stored at different conditions (23°C and 37°C; with and without immersion in water) for 24 hours. DTS was tested with a Universal Testing Machine (AGS-X series, Shimadzu, Japan) and surface microhardness was tested with a Vickers Microhardness tester (HMV-G Series Micro Vickers Microhardness Tester, Shimadzu, Japan). Data were analyzed statistically using a one-way ANOVA test (and Shapiro-Wilk test. Result: The values of microhardness and DTS increased significantly both for the composite resin alkasite and zirconia-reinforced GIC with increasing temperature in the groups without immersion. However, there was a significant decrease in microhardness and DTS after immersion in distilled water at 37°C for both the composite resin alkasite and zirconia-reinforced GIC. Conclusion: It can be concluded that storage conditions affect the microhardness and DTS of resin composite Alkasite and Zirconia-reinforced GI
THE CORRELATION BETWEEN DENTAL CARIES AND ORAL HEALTH RELATED QUALITY OF LIFE AMONG VISUALLY IMPAIRED CHILDREN
Get PDFBackground: Visually impaired children have a low vision to visualize the process of plaque removal on teeth resulting in dental caries progression. Dental caries has an impact on children’s quality of life. The impact of dental caries on oral health related quality of life among visually impaired children remains mostly unknown. This study aimed to determine the correlation between dental caries and oral health related quality of life (OHRQoL) among visually impaired children.Method: This study was an observational study with a cross sectional design and conducted across four regencies in Yogyakarta and Central Java Province. A total of 70 visually impaired children aged 7-18 participated in this study. The participants underwent a dental examination to determine their caries experience using DMFT/dmft index. Oral health related quality of life was measured using the COHIP SF 19 questionnaire with interviewing method.Result: Data analysis showed that caries frequency was 94,3%. The mean number of DMFT/dmft index was 4,8 ± 2,743. Spearman correlation test showed the p value was 0,611 (p>0,05) for the correlation between DMFT/dmft index and OHRQoL.Conclusion: This study showed no correlation between dental caries and oral health related quality of life among visually impaired children
SCHOOL-BASED FLOURIDE MOUTH RINSE (S-FMR) SEBAGAI UPAYA PENCEGAHAN KARIES PADA ANAK: SEBUAH TINJAUAN PUSTAKA
Get PDFBackground: Dental caries is an infectious microbial disease that begins as demineralization of an inorganic portion of tooth, followed by the destruction of organic parts, leading to cavity formation. Fluoride helps to prevent caries which can be administered systematically or topically. Fluoride mouth rinse has been used in school program to prevent caries.Method: This article aim is to describe the effectiveness of school-based fluoride mouth rinse (S-FMR) as one of the effective methods to prevent caries on children.Discussion: The School-based Fluor Mouth Rinse (S-FMR) program or gargling using fluoride in schools is the use of fluoride as a mouth rinse for children aged 6-18 years conducted in the school environment. Several studies have shown that S-FMR can reduce the prevalence of caries.Conclusion: S-FMR is considered effective in preventing caries due to the simple method, cost-effectiveness, time efficiency, and easily undertaken by the teacher’s supervisio
COWHIDE GELATIN NANOPARTICLES AND TITANIUM-PREPARED PLATELET-RICH FIBRIN POTENTIAL IN PERIODONTITIS HEALING PROCESS
Get PDFIntroduction: Based on epidemiological data, the periodontitis prevalence in Indonesia reached 96.58%. Periodontitis is a periodontal disease caused by specific microorganisms causing attachment loss, alveolar bone resorption, and periodontal pocket. The pocket which is more than 6 mm couldn’t be carried out using standard care. Therefore bone grafting is needed to fix the bone defect. Cowhide Gelatin nanoparticle (GNPs) combined with Titanium-Prepared Platelet-Rich Fibrin (T-PRF) is an innovative healing of periodontitis. Objective: To review the potential of cowhide gelatin nanoparticles combined with titanium-prepared platelet-rich fibrin (T-PRF) as an innovation in the healing of periodontitis defects. Literature review: Cowhide GNPs are the main ingredients for the bone replacement, which are nano-sized, biocompatible, bioactive, osteoconductive, non-toxic, and have a porous structure that supports the drug-delivery process. T-PRF contains platelets, fibrin, growth factors that can stimulate bone regeneration. Discussion: GNPs support the attachment and proliferation of osteoblast cells and the formation of mineralized bone matrix. The contents of T-PRF, which are fibrin, platelets, and growth factors, one of TGF-β1 will produce bone formation and periodontal healing in the large number. Moreover, T-PRF has leukocyte-fibrin, which may give an antibacterial effect. Thus, GNPs combined with T-PRF have more potent properties that support each other in carrying out their functions to stimulate the healing process. Conclusion: The combination of cowhide gelatin nanoparticles (GNPs) and titanium-prepared platelet-rich fibrin (T-PRF) has the potential to be effective in bone regeneration in periodontitis defects
ANTIBACTERIAL EFFECT OF WHITE POMEGRANATE PEEL EXTRACT (PUNICA GRANATUM L) AGAINST STREPTOCOCCUS SANGUINIS
Get PDFBackground: Streptococcus sanguinis is one of the bacteria that plays role in the occurrence of caries, acts as an anchor for the attachment of other oral microorganisms that will form dental plaque in the development of caries. The extract of White Pomegranate peel has several compounds such as flavonoids and tannins that have potential as an antibacterial. Objective: To analyze the antibacterial potency of white pomegranate peel extract against Streptococcus sanguinis bacteria. Methods: Using disc diffusion method (Kirby-Bauer) with 7 treatment groups (n=4). Streptococcus sanguinis bacteria is inoculated in agar medium on petri dish, dripped 50 μl to each disc with 100%, 50%, 25%, 12,5%, 6,25% concentration of pomegranate skin extract, and control group. The petri dish is placed in the incubator at 37oC for 24 hours. Each of antibacterial is observed with the presence of inhibition zones around the disc. The analysis uses Kruskal-Wallis and followed by the Mann-Whitney test. Results: There are inhibitory zones for all treatment concentrations, except for concentration of 12.5%, and 6.25%. There is a significant difference in the concentration of pomegranate skin extract towards the growth of Streptococcus sanguinis bacteria. Conclusion: White pomegranate skin extract has antibacterial potency at 25%, 50%, and 100% concentratio
DISCLOSING AGENT FROM RED DRAGON FRUIT PEEL AS DENTAL PLAQUE INDICATOR
Get PDFBackground: Red dragon fruit peel mucoadhesive gel has the potential to dye dental plaque. The colour of a transparent dental plaque can be seen with visualize using dyes (disclosing agent). One of the natural products containing dyestuff betacyanin, namely the peel of the red dragon (Hylocereus polyrhizus). This research aimed to review the peels of red dragon betacyanin (Hylocereus polyrhizus) in the mucoadhesive gel formula as an alternative disclosing agent.Method: Research is done with the manufacture of thick peels of a red dragon extract with the maceration methods. In clinical trials carried out an application red dragon fruit peel mucoadhesive gel 10%, concentration 15 % and Three-Tone Plaque Disclosing Gel. The assessment of plaque was carried out using the O’leary method. Result: The results obtained were analyzed by the t-test method. The mean of plaque index scores with red dragon fruit peel mucoadhesive gel concentrations of 10% (22.08), 15% (26,29) and Three-Tone Plaque Disclosing Gel (66,21; 64,20). The effectiveness of the gel with a concentration of 10% is 33.87%, and 15% concentration is 40.32%. Conclusion: The mucoadhesive gel of red dragon fruit peel has the potential to colour dental plaque
STUDY ANALYSIS OF ORAL TUBERCULOSIS LESION IN SOUTH KALIMANTAN, INDONESIA
Get PDFBackground: Tuberculosis is an infectious disease which is the second-largest cause of death in the world after HIV / AIDS. Tuberculosis caused by Mycobacterium tuberculosis that usually attacks the lungs but can manifest in the oral mucosa. Clinical manifestations of oral Tuberculosis related to age, sex, duration of treatment, systemic disease, and Multi-Drug Resistant therapy. There is no research on descriptive studies of oral manifestations of tuberculosis patients in South Kalimantan, specifically in Banjarmasin. This study aims to analyze the clinical manifestations of oral Tuberculosis descriptively in South Kalimantan, Indonesia. Method: This was a descriptive analysis with cross-sectional study design, based on history and clinical oral examination. It was used 30 respondents by purposive sampling method. Data were analyzed by Chi-square (p0.05). The clinical manifestations of oral Tuberculosis are xerostomia, candidiasis, and angular cheilitis.Conclusion: The duration of treatment and the systemic diseases are associated with xerostomia and candidiasis in the oral cavity of TB patients