ODONTO : Dental Journal
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THE EFFECT OF PEGAGAN GEL (CENTELLA ASIATICA (L.) URBAN) ON WOUND HEALING PROCESSES IN MICE (MUS MUSCULUS) IN VIVO
Get PDFBackground : Tooth extraction and incision are procedures that are often performed in dentistry that cause injury and damage to oral tissues. Injuries to the oral tissue must be treated immediately so as not to interfere with the stomatognathic system. Pegagan (Centella asiatica (L.) Urban) is one of the medicinal plants used to accelerate wound healing. The purpose of the study was to determine the effect of giving extract pegagan gel on the oral mucosal wounds of mice (Mus musculus) in various concentrations on the number of fibroblast cells.Methods : The research method used experimental post test only control group design. Data analysis used One Way Anova test and Least Significant Difference (LSD) test. The experimental used were 24 male mice aged 6-8 weeks weighing 20-25 grams and healthy, divided into 4 (four) groups. The extraction wounds were smeared with 5%, 10%,15% concentration of pegagan extract gel and the placebo was smeared for 1 minute and done twice a day. Experimental animals were decapitated on day 5 without pain and continued with the stage of making microscopic preparations of tissue taken and fixed with buffer formalin 10% for a maximum of 24 hours.Results : The results showed that the average number of fibroblasts in the administration of extract pegagan gel was 15% with 400 cells, 10% was 291 cells, 5% was 262 cells and the average CMC Na gel group was 74 cells.Then continued with the One Way Anova test and the Least Significant Difference (LSD) test and results showed the average number of fibroblast cells in the four groups after being given treatment was significantly different with a significant level (Ï) < 0.05 and followed by a post hoc test, it is known that the gel extract pegagan 5%, 10%, and 15% can increase the number of fibroblast cells.Conclusion : Extract pegagan gel 15% more increased the number of fibroblast cells than extract pegangan gel10% and 5%
CHEWING XYLITOL CANDY AND PROBIOTIC CANDY ON THE GROWTH OF DENTAL PLAQUE AND NUMBER OF COLONY OF STERPTOCOCCUS MUTANT BACTERIA
Get PDFBackground: Caries or cavities is a disease in the hard tissue of the teeth characterized by damage to enamel and dentin caused by the metabolic activity of bacteria in plaque which can cause demineralization due to the products of microorganisms. Xylitol gum and probiotics can be used to reduce the plaque index and the number of Streptococcus mutans colonies. The purpose of this study was to compare the effect of chewing gum containing xylitol and probiotics on plaque growth and to determine the number of Streptococcus mutans colonies in students of the Faculty of Dentistry, Sultan Agung Islamic University.Method: This study used an experimental research method with a randomized posttest only control group design used 30 respondents divided into 3 groups, namely the xylitol, probiotics, and sugar groups. Each group had a good oral hygiene index (OHIS) and then checked the plaque index and the number of Streptococcus mutans colonies before and after chewing gum for 1 minute. Data were analyzed in the Laboratory of Microbiology, Faculty of Medicine, Sultan Agung Islamic University. Data were analyzed using paired t-test and Wilcoxon test. Result: The p value <0.05 or there was no significant decrease in each group. Conclusion: There is an effect of chewing gum containing xylitol and probiotics on the number of Streptococcus mutans and plaque index, but there is no significant difference between the plaque index and the number of Streptococcus mutans colonies
KNOWLEDGE ON INFECTION PREVENTION AND CONTROL AMONG STUDENTS IN UNIVERSITAS PADJADJARAN DENTAL HOSPITAL
Get PDFBackground: Dentist is one of the professions that are vulnerable to the transmission of infectious diseases there is hence it requires proper infection control. The educational hospital is responsible for carrying out infection control training that includes appropriate standard precautions for dental students before carrying out educational activities in the hospital so that students are ready to treat patients. This study aims to determine the knowledge of interns and residents at Universitas Padjadjaran Dental Hospital on infection prevention and control.Method: This is a quantitative descriptive study with a cross-sectional approach using data from the training section of Universitas Padjadjaran Dental Hospital from August 2020 to February 2021. Determination of the number of samples taken using the Isaac and Michael formula and using a proportionate stratified random sampling technique.Result: The average value of interns and residents are the same, which is 71, seen also from the average percentage of those who answered correctly on the questionnaire which showed similar results between interns and residents. Conclusion: Knowledge on infection prevention and control among interns and residents are the same, with knowledge classified in the sufficient category
The effect of casein and lactoferrin of bovine's milk on nestin expression of the exposed dental pulp
Get PDFBackground: Pulp tissue can regenerate if damage occurs. The pulp differentiates into new odontoblast-like cells in Progenitor cells. Nestin is a marker of the presence of new dontoblast-like and expressed in pathological conditions. Bovine's milk casein and lactoferrin contain proteins that accelerate cell growth. This study aimed to determine the effect of casein and lactoferrin on the expression of nested cells in the exposed dental pulp.Method: The research method is an experimental laboratory with male 30 Spraque Dawley rats. Rats were grouped as : casein, lactoferrin, casein-lactoferrin, and Ca-(OH)2. Each group consisted of 15 samples of molars which were divided into 5 samples of teeth for observation on the 7, 14, and 21 days. The first molars of rats were prepared until the pulp was exposed. The data of nestin quantity with immunohistochemical (IHC) analysis on histological preparations were analyzed using two-way ANOVA then the least significance difference (LSD) test.Result: The results of the average number of nested cells on 7,14 and 21 days were highest on combination of casein and lactoferrin. Two-way ANOVA statistical proved that there was a significant difference (p<0.05) in each observation period and group and the number of expressions of nestin cells. LSD test showed that there was a significant difference (p<0.05) between groups compared to the control group.Conclusion: Combination casein and lactoferrin extracts affect the expression of the number of nestin cells as a marker of new odontoblast-like cells in the exposed dental pulp
Cytotoxicity test of apple cider vinegar as a root canal irrigant against fibroblast cells
Get PDFBackground: Apple cider vinegar potentially can be used as an alternative to irrigation solutions because of its antibacterial compounds that can inhibit Enterococcus faecalis, a pioneer bacteria that cause root canal treatment failure. One of the ideal irrigation solution requirements is that it isn’t toxic to oral cavity tissues, so it’s necessary to run a cytotoxicity test on apple vinegar solution. Cytotoxicity test is the initial part of the evaluation of a dental material before it can be used by humans. Cytotoxicity test was performed on fibroblast cells because the irrigation solution can contact with fibroblast, which are the main cells in the periodontal ligament around the apical. The purpose of this study was to analyze the in vitro cytotoxicity effect of ACV on fibroblast cells.Method: Apple vinegar with concentrations of 0.31%, 0.63%, 1.25%, 2.5%, and 5% was tested using the MTS assay method.Result: The results showed that there was a cytotoxicity effect of apple vinegar solution as a root canal irrigation agent against fibroblasts cell. Apple cider vinegar with concentrations of 1.25%, 2.5%, and 5% are potentially toxic because the percentage of cell viability is less than 70%.Conclusion: There is a cytotoxicity effect of apple cider vinegar solution as a root canal irrigant on fibroblast cell
Knowledge description of adolescent ages 14-18 years about side effects of using fixed orthodontics
Get PDFBackground: The use of fixed orthodontics is not a new thing for the community. The rapidly increasing information has changed people's understanding of the use of fixed orthodontic appliances where the initial function of fixed is for health, but along with changes in one's knowledge, the use of fixed orthodontics has turned into a style or trend that aims to make the appearance more attractive. Most of the youth are currently doing fixed orthodontic treatment only for a lifestyle, trend or style without knowing the complaints and goals of fixed orthodontic treatment. In addition, most teenagers do not know the orthodontic treatment that should be carried out by orthodontic specialist dentists. This study aims to specifythe description of adolescents aged 14-18 years in SMA Negeri 1 Belitang, South Sumatra province regarding the side effects of using orthodontic appliances.Method: This is a descriptive observational using a cross sectional study. The sampling technique used is total sampling with a total of 50 respondents.Result: The results of the study revealed that adolescents' knowledge of the side effects of using fixed orthodontic appliances was in the good category of 13 subjects (26%), sufficient category 29 subjects (58%), and less category 8 subjects (16%).Conclusion: The conclusion of this study is that the knowledge possessed by adolescents aged 14-18 years at SMA Negeri 1 Belitang, South Sumatra Province regarding the side effects of using orthodontic appliances is quite good in as many as 29 subjects(58%)
Binahong leaf extract (anredera cordifolia) mucoadhesive patch as an alternative therapy for recurrent aphthous stomatitis
Get PDFBackground: Recurrent Aphthous Stomatitis (RAS) is one of the most common oral diseases of all ages. There has been no study that defines the leading cause of RAS. The utilization of binahong leaves (Anredera cordifolia) can be beneficial for the treatment of RAS as it acts as an anti-inflammatory and accelerates the proliferation of fibroblasts. The combination between binahong leaves and the mucoadhesive patch will increase the effectiveness of RAS treatment.Method: Literature study through PubMed and Google Scholar databases was conducted using PRISMA flow chart looking for the efficacy of binahong leaves (Anredera cordifolia) for mucosa wound healing and mucoadhesive patches for the treatment of RAS.Result: The search results found 31 articles that met the criteria for analysis. Flavonoids and saponins in binahong leaves extract act as an anti-inflammatory and increase the proliferation of wound healing. Mucoadhesive patch formulation can increase the effectiveness of RAS treatment on the mucosa.Conclusion: Binahong leaves extract in the form of mucoadhesive patches can be used as an alternative treatment for Recurrent Aphthous Stomatitis
EFFECT OF FIBROIN SPONGE ON ALVEOLAR BONE REMODELING PROCESS POST TOOTH EXTRACTION
Get PDFBackground: There are still quite a lot of cases of tooth extraction in Indonesia because of the cavities are not treated or because of other cases. Extraction is the main cause of 90% of bone destruction and a risk of infection and bleeding. Fibroin sponge is a natural polymer material derived from Bombyx mori silk cocoon which contains of 18 amino acids and have a good mechanically, biodegradable and biocompatible. This study aimed to determine the effect of fibroin sponge on the remodeling of post tooth extraction sockets.Method: This study was a laboratory experiment with quasy experimental design using 30 Sprague dawley rats divided into 2 groups. Each group had the mandibular left incisor extracted. The first group as a control was given 15 ug of Curaspon and the second group was given 15 ug of fibroin sponge intra alveolar.Result: The results were observed after the 3rd, 7th, 14th, 21st, 28th days under a light microscope and identified by scoring bone remodeling based on predetermined criteria. Data were analyzed using the Mann-Whitney Test followed by the Kruskall-Wallis Test. The results showed that there was no difference in the process of bone remodeling post tooth extraction in the fibroin sponge group compared to Curaspon (p>0.05).Conclusion: Fibroin sponge has the same effect on the bone remodeling process as Curaspon
HERPES ASSOCIATED ERYTHEMA MULTIFORME (HAEM) : CASE REPORTS
Get PDFBackground: Erythema Multiforme (EM) is a mucocutaneous inflammatory reaction with clinical manifestations ranging from mild to severe which are self-limiting but can also be life-threatening. EM is considered an immunological disease in the form of a hypersensitivity reaction caused by an immune response to certain antigens such as herpes simplex virus known as Herpes Associated Erythema Multiforme (HAEM) or certain types of drugs known as Drug Induced Erythema Multiforme (DIEM). Hematinic factors greatly affect the length of the healing process in cases and can also worsen anemia because there is a tendency for bleeding on the lips. Case and Management: This case report discusses the case of EM in a 41-year-old woman who came to the practice with complaints of canker sores and scabs on her lips, disappearing and bleeding easily. The patient's condition is accompanied by chronic anemia with results of Hb, MCV, MCH, MCHC (below the reference value). Anti HSV IgG examination was 44.2 (reactive) so that HAEM was diagnosed with chronic anemia. Pharmacological management was given paracetamol, acyclovir, folic acid, Fe, and vitamin B12. Lip lesions are given a blended ointment. Provided education and instructions to patients to maintain nutritional intake, adequate rest and regular use of drugs. Conclusion: The healing condition can be faster by paying attention to the chronic anemia condition experienced by the patient
RELATIONSHIP BETWEEN NUMBER AND REGION OF TOOTH LOSS WITH THE QUALITY OF LIFE IN THE ELDERLY.
Get PDFBackground: Prevalence of tooth loss due to extraction is highest in the elderly aged 65 years and over. Tooth loss means loss of tooth function therefore it affects the quality of life. It seems a reflection of a person's comfort and disability in terms of limited physical, psychological, and social functions. The aim of this study was to determine the relationship between number and region of tooth loss with the quality of life in the elderly at the Margo Mukti Elderly Social Service Home, Rembang.Method: Research design was observational study with cross sectional approach. Sample size was 58 elderly people taken from the total population of 300 elderly who live at social services home. Data was collected by examining the number and region of tooth loss followed by interviews using the Oral Health Impact Profile (OHIP-14) questionnaire. Data analysis was performed by univariate and bivariate analysis, using the test chi square and Kolmogorov Spirnov to determine the relationship between independent and dependent variables.Result: The results showed significance of statistical was found at relation number and region of tooth loss with quality of life (0.006). The highest number of tooth loss was in the range of 6-23 teeth. Anterior region was the most tooth lost. Most of elderly (67.2%) have good quality of life.Conclusion: There is relationship between number and region of tooth loss with quality of life related to oral healt