Indo. J. Chem. Res.
Not a member yet
303 research outputs found
Sort by
Development of Differential Pulse Anodic Stripping Voltammetry Technique for Cadmium(II) Detection and Its Application in Water Spinach
Get PDFCadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 µM~0.0771 ppm and 0.5525 µM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg
Antibacterial Effectiveness of Syzygium cumini (L.) Skeels Leaves to Escherichia coli pBR322
Get PDFBacterial resistance is a serious problem that until now still has become a global problem. The search for new antibacterial compounds is needed to overcome this problem. Syzygium cumini (L.) Skeels is a plant known to contain bioactive compounds that can be used as an antibacterial agent. This study aims to explore the leaves of this plant as an antibacterial against Escherichia coli pBR322 resistant bacteria. Based on the antibacterial test results, extracts and leaf fractions of this plant can inhibit bacterial growth. Ethyl acetate fraction at a concentration of 800 ppm showed strong antibacterial activity with an inhibition zone diameter of 10.36±0.02 mm followed by ethanol extract and other fractions, namely 8.43±0.01 mm (ethanol extract), 7.24±0.01 mm (water fraction), and 1.45±0.01 mm (n-hexane fraction). The results of spectrophotometric MIC determination also showed inhibition against bacterial growth, which was marked by a decrease in the absorbance value at the lowest antibacterial concentration of 600 ppm from 0.454 to 0.070 at wavenumber 600 nm after 24 hours of incubation. The decrease in the absorbance value indicated that the antibacterial properties of the plant leaves at this concentration were effective in inhibiting bacterial growth
Recovery of Gold in Au/Cu/Mg System from SH/Fe3O4@SiO2 as a Magnetically Separable and Reusable Adsorbent
Get PDFThe recovery of Au(III) in the Au/Cu/Mg system from mercapto-silica hybrid coated magnetite (SH/Fe3O4@SiO2) adsorbent has been investigated. This adsorbent characterized using FT-IR to determine functional groups, crystallinity study using XRD, surface morphology using SEM, material compositions with XPS, surface area using nitrogen adsorption, and TGA to study thermal stability. Adsorption of metal ions carried out with batch system for 30 minutes at a pH of 3. In the Au/Cu/Mg multi-metal system, Au(III) ions were easily desorbed (approximately 85%) by SH/Fe3O4@SiO2 adsorbent based on HSAB (Hard Soft Acid Base) theory that Au(III) ion is a softer metal than Cu(II) and Mg(II) where Au(III)>Cu(II)>Mg(II). The recovery of Au(III) ions was easily desorbed using thiourea 7% in 0,1 M HCl solution with the percentage of 79%. The process of SH/Fe3O4@SiO2 adsorbent separation after adsorption and recovery was very easy. The adsorbent could perfectly separate in 5 minutes using an external magnet. The SH/Fe3O4@SiO2 adsorbent can be reused on the adsorption-desorption process of Au(III) in the Au/Cu/Mg system approximately four times of cycle reactions
Bioconcentration of Chrome (Cr) Metal in Three Types of Sponge (Callispongia sp., Xetospongsia sp. and Petrosia sp.) from Ambon Bay
Get PDFResearch about bioconcentration of Chrome metal in three types of sponge (Callispongia sp., Xetospongia sp., and Petrosia sp.) from Ambon Bay. This research main is to know the most effective type to accumulate Cr metal in water. The Cr concentration measurement of water, sediment, and sponges at each location shows that metal concentration is 0.001-0.030 ppm in sediment higher than 0.001-0.003 ppm in water. Concentration based on sponges type Callispongia sp., Xetospongia sp., and Petrosia sp. are 0.001-0.262 mg/kg dry weight: 0.001-0.134 mg/kg dry weight, 0.001-0.330 mg/kg dry weight, respectively. The adsorption factor (AF) of Cr metal ranges from 0.000-0.028 ppm. Bioconcentration factor (BCF) based on sponges type Callispongia sp., Xetospongia sp., and Petrosia sp. are -0.011-0.261 mg/kg dry weight, -0.001-0.132 mg/kg dry weight, and -0.001-0.329 mg/kg weight dry, respectively. Sponges type that suitable used as Cr metal pollution monitoring media is Callispongia sp. and Xetospongia sp
Qualitative Protein Hydrolyzed from Nerita undata in Supralittoral Rocks and Mezolittoral Zone of Hasa Cape Using TLC
Get PDFGastropods are the sources of protein for coastal communities in the Maluku islands. We conducted analysis quantitative and qualitative of protein in Nerita undata meat from Hasa Cape in Saparua Island. The percentage of protein was analyzed by the Kjeldahl method. Qualitative analysis began with breaking peptide bonds in protein to amino acid components by sulphuric acid and barium hydroxide hydrolyzed. We identified amino acid compounds by using thin-layer chromatography (TLC) in butanol-acetic acid-water (8:1:1, v/v) as eluent. The result showed that N. undata meat contains 11.15% of protein and twelve amino acid compounds. There are seven essential amino acids in N. undata meat from supralittoral rocks and mezolittoral zone of Hasa cape is a source of quality protein. Thus, this species can be considered as a source of high-quality protein
Morphological Observation, Identification and Isolation of Tropical Marine Microalgae from Ambon Bay, Maluku
Get PDFMicroalgae are photosynthetic microorganisms that are widely distributed in waters. They have the potential as an alternative source of pigments, lipids, proteins, and carbohydrates. In this study, identification and isolation of single-cell microalgae from Ambon Bay were carried out by serial dilution method and inoculation of the culture by appearance/spread on the surface of the solidified agar medium. The results showed that the waters of Ambon Bay have physical parameters such as temperature, pH, salinity, and TDS, which are still within the threshold specified in the seawater quality standard for biota. The water conditions at the 10 sampling locations had different salinity, pH, temperature, and TDS. This condition affects the distribution of microalgae in the waters in Ambon Bay. The image from light microscopy shows that there are 27 species of microalgae scattered in the waters of Ambon Bay, which belong to the classes Chlorophyceae, Flagilariophyceae, Bacillariophyceae (diatoms), Coscinodiscophyceae, and Dinophyceae. Microalgae isolation obtained five species (single-cell) including four species from the class Bacillariophyta (Navicula sp, Nitzschia sp, Cyclotella sp) and one species from the class Chlorophyta (Chlorella sp)
Adsorption and Characterization of Activated Sugarcane Bagasse Using Sodium Hydroxide
Get PDFSugarcane Bagasse can be used as an adsorbent both under natural conditions and modified by chemical activation using sodium hydroxide (NaOH). Activation of sugarcane bagasse with NaOH was carried out at variations of 5:1, 10:1, and 20:1 (w/w). The absorption ability of bagasse adsorbent to methylene blue solution was carried out with the parameters of variation of contact time (60, 90, 120, 150, and 180 minutes), adsorbate concentration (20, 30, 40, 50, and 60 ppm) and temperature (30, 40, 50, and 60 oC). The adsorbent's characterization included determining the functional groups using FTIR, morphology, and mass of elements using SEM-EDX, and determining the surface area and volume of adsorbent pores using the BET methods. The highest adsorption percentage results were found in the NASB10:1 adsorbent at 99.50%. The optimum conditions for the NASB10:1 adsorbent are with a contact time of 120 minutes, an adsorbate concentration of 50 ppm and a temperature of 30 oC or 303 K. The NASB10:1 adsorbent has the highest surface area compared to other adsorbents, namely 2.803 m2/g so that it can perform the maximum absorption of methylene blue
The Computational Calculation and Molecular Docking of Aeroplysinin-1 As Antibacterial
No full textAeroplysinin-1 is naturally found from marine sponges as an anti-bacterial compound. Computational calculation and molecular docking were performed for aeroplysinin. Aeroplysinin as an inhibitor has optimized in the gas phase using DFT with 6-31G(d) functional. The structure from geometry optimization of aeroplysinin-1is, not in one plane. The interaction of aeroplysinin-1 with two different DNA gyrase from E. Coli and S. Aureus. In this research,aeroplysinin-1 can inhibit the protein with the free binding energy of about -5.7 kcal/mol and -6.35 kcal/mol, respectively, for E. Coli and S. Aureus. The dominant molecular interaction is the hydrogen bond
The Ouw Natural Clay Impregnation Using Titanium Dioxide as a Rhodamine B Dyestuff Degrader
No full textThis study aims to determine the effect of calcination temperature on Titanium dioxide impregnated Ouw Natural Clay (LAO) on the degradation of Rhodamine B dye. The calcination temperature used was 200, 300, and 400 °C. Their radiation was carried out with 3x8 watt UV lamps and sunlight. The degradation percentage of Rhodamine B dye produced by LAO-TiO2 calcined at a temperature of 200, 300, and 400 °C with sunlight were 98.80%, 98.87%, and 96.07%, respectively. The degradation percentage of Rhodamine B dye produced by LAO-TiO2 calcined at temperatures of 200, 300, and 400 °C with the help of UV light were 96.01%, 97.41 %, and 97.18%, respectively
Study of UiO-66 and UiO-66 Modulated with Acetic Acid as the Adsorbent for Eriochrome Black T Dye
Get PDFUiO-66, as one of the metal-organic framework (MOF) compounds, has been used to treat some anionic and cationic dye waste. In order to determine the adsorption selectivity decisively, the synthesis of UiO-66 and UiO-66 modulated with acetic acid had been carried out, along with their adsorption tests for Eriochrome Black T (EBT) dye solution. The synthesis was performed by utilizing a solvothermal method with the reaction mixtures of zirconium (IV) chloride (ZrCl4) and terephthalic acid (H2BDC) as a ligand heated at 120 oC for 24 hours. Both UiO-66 (without acetic acid) and acetic acid modulated UiO-66 were obtained as a white powder. Acetic acid as a modulator was added and being investigated for the adsorption capability compared to the normal UiO-66. This study showed that normal UiO-66 exhibited better adsorption than acetic acid modulated UiO-66 with a mmol ratio of acetic acid:ligand varied from 50:1, 100:1, and 150:1. Acetic acid modulated UiO-66 with a mmol ratio of 50 exhibited the best crystallinity as observed by using x-ray diffraction. It can be concluded that the adsorption of EBT using normal and acetic acid modulated UiO-66 obeyed the pseudo-second-order reaction rate law as well as the Langmuir adsorption isotherm pattern