Jurnal Agroekoteknologi
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PERTUMBUHAN TANAMAN KANTONG SEMAR (Nepenthes rafflesiana Jack.) DENGAN MODIFIKASI KONSENTRASI MEDIA DAN pH SECARA IN VITRO
This research about growth of pichter plant Nepenthes rafflesiana Jack was aimed to determine the modification medium’s concentration and pH at in vitro . This research has been conducted in the laboratory of plant concervation center Bogor Botanical Garden. This research was doing from Juni to September 2010. research using randomized block design with 2 factor, the first factor is modification concentration medium MS, consisting of 5 level is 1 MS, 1/2 MS, 1/4 MS, 1/8 MS, dan 1/16 MS; and the second factor is the pH medium was consisted 3 level: pH 4,7, pH 5,7 dan pH 6,7 was repeated four times. The results showed that concentration medium 1/8 MS and all pH medium were significant the variable of number pichter, number rootlet, percentage of planlet life, percentage of planlet pichter, percentage of planlet rootlet.Key words: Nepenthes rafflesiana Jack, medium concentration, pH
SKRINING RHIZOBAKTERIA PENGHASIL INDOLE ANCETID ACID PENGINDUKSI PERTUMBUHAN DARI RHIZOSFIR CABAI (Capsicum annuum L.)
Rhizobacteria was a colonialist bacterial at rooting area that had capacity to produce IAA and enhance plant growth. Rhizobacteria indigenus chili had ability to produce IAA and induces various of plant growth. IAA production was not always linked with the ability to enhance plant growth. Obtained there were 11 isolates with various of morphological characters and had ability as RMSD (Plant Growt Promoting Rhizobacteria) with an effective increase in growth from 57.88 to 82.80%.Key words: Rhizobacteria, IAA, Growth, Chili Rhizosfe
REGENERASI BEBERAPA EKSPLAN TANAMAN JAMBU BIJI (Psidium guajava L.) PADA MEDIA KOMBINASI BAP DAN IBA SECARA IN VTRO
The experimen was done to know the best combination of the treatment with the used of different types of explan conducted in Tissue Culture Laboratory, 633 Biotechnology Building - Center for the Assessment and Application of Biotechnology (Biotech Center of BPPT), Agency For The Assesment and Application of Technology (PUSPIPTEK), Serpong July to October 2009. The results of this study is expected to be used as a reference in the process of guava rapid regeneration (in vitro) in order to support quality improvement activities of the guava plant. Research used Completely Randomized Designed (RAL) with 3 factors, BAP concentrations level (0; 2.5; 5; 7.5 and 10 mg/L), IBA concentrations level (0 and 0.5 mg/L) and types of explan (cotyledon, hipokotil and tip). Results showed that combination treatment of 0 mg/L BAP and 0.5 mg/L IBA was good for callus initiation hipokotil and cotyledon at 2 weeks after palnting, diameter of callus in the cotyledon and the number of roots. Treatments combination which most effective in inducing shoot growth is the tip treatment with the concentration of 10 mg/L BAP and 0.5 mg/L IBA in 6 weeks after planting. Concentration of 2.5 mg/L BAP and 0.5 mg/L IBA is a combination of the best growth regulator substances to the shoot height, number of leaves and node. Provision of BAP, IBA and Explan either single or interaction between the second and third generally provide a significant effect on the growth of Psidium guajava L.Key words: guava plant, Psidium guajava L., BAP, IB
PERGESERAN KOMPOSISI GULMA PADA TANAMAN PAPAYA (Carica papaya) YANG DIBERI PUPUK ORGANIK DAN ANORGANIK
ABSTRACT This study aims to see a shift in the composition of the weed on papaya plants fed organic fertilizer (pukana) and inorganic (NPK). And analyze its effect on dry weight of weeds. Research done at the Garden Village folk Sukasari Kec. Talang Kelapa, Palembang, South Sumatra, from June to October 2010. Randomized studies using Design Group (RAK) with 5 treatments and 5 replications. Fertilizer treatments consisted of: P1 = 30 kg plant pukana -1, P2 = 100 g NPK tanaman-1, P3 = 30 kg + 100 g of NPK plant pukana -1, P4 = 25 kg + 200 g of NPK plant pukana-1, P5 = 20 kg + 300 g of NPK plant pukana -1. To determine the dominant weeds were analyzed by the method of squares. The results showed that a shift in the composition of the weeds before cultivation and after treatment fertilizer age 1 month, 2 months and 3 months. Before tilling the land is dominated by Imperata cylindrica, Cyperus rotundus and, Mimosa invisa, while after treatment is dominated by the weed Ageratum conyzoides and Echinochloa colonum both at age 1, 2 and 3 months after planting. The results of the diversity of the highest weed dry weight obtained in the treatment of P3 is 81.85 g and the lowest in the treatment of P2 with weight 37.85 g.Keywords: Shifts, Weeds, Papaya, Pukana, NP
KOREKSI KESESUAIAN LAHAN DENGAN REFERENSI KESESUAIAN SUHU PERTANAMAN KENTANG MERAH PADA DATARAN SEDANG BENGKULU
ABSTRACTAdaptation red potato planted on medium altitude in Bengkulu upland in order to correct land suitability classification based on criteria of land characteristics/quality. The research was conducted from July to October 2009, Kepahiang, Bengkulu located in 4 (four) experimental stations; mediumaltitude which located in Kelobak 525 m (up sea level, usl) and Pematang Donok, 650 m usl comparing location in Tugu Rejo 1135 m usl and Bandung Baru 1346 usl. Temperature in high altitude on Bengkulu upland was 22.3 oC in Bandung Baru and 23.5 oC in Tugu Rejo. In highland Bengkulu, land suitability class was Marginally Suitable (S3t) around Bandung Baru, while around Tugu Rejo, and medium altitude of upland Bengkulu around Kelobak and Pematang Donok with average temperature 24.8 oC categorized Permanently Non Suitable (N2t). Following the result of red potato adaptation research, there was no significantly diference among 4 (four) experimental location of potato yields in which yield of potato in Bandung Baru was 16.7 tons ha-1, Tugu Rejo was 15.5 ton ha-1, and yield of red potato in medium altitude of Bengkulu upland in Kelobak was 15.4 tons ha-1 and Pematang Donok was 19.3 tons ha-1. Based on those, temperature as a limiting factor for potato growth was the same suitable for red potato cultivation in the high and medium altitude of Bengkulu upland. Criteria of land sutability classification for potato cultivation in medium altitude of Bengkulu upland categorized N2t, Permanently Unsuitable should be corrected because of medium altitude of upland in Bengkulu high potential to develop as area for red potato cultivation.Key words: temperature, land suitability classification, red potato
EFEK SUSPENSI PENGAWET TERHADAP KUALITAS BUNGA POTONG HERBRAS
ABSTRACTThe usage of preservative solution as a force to improve the quality of Transvaal daisy cut flower Ornella cultivar is effective. A preservative that was formulated from sucrose + citric acid + lysol + oxyacetate acid was made as suspension by adding of cmc, will be examined its effectivity in this experiment. An experiment by using Randomized Block Design bifactor, which are dosage of suspension and suspension saving period before it was going to be added into the soaking water of Transvaal daisy cut flower. Respons of variable which analyzed are vase life, petal blooming capacity, dynamics of fresh weight relative and water absorpsiton relative 2-days. The result showed that dosage a 50 mLL-1 of suspension and three months saving period was the best compare to another respons variable.Keywords: Cut flower preservative, Dosage suspensio
IDENTIFIKASI CENDAWAN TERBAWA BENIH PADI DARI KECAMATAN CIRUAS KABUPATEN SERANG BANTEN
ABSTRACT The objective of this experiment was conducted to identification of seed-borne fungi that infected or contaminated paddy seed. Paddy seed samples were collected from sub district of Ciruas, Serang, Banten. Eighty hundred seeds used by random sampling method as the work sample in seed health testing were obtain from 8200 gram of the sample that collected from 82 group of farmer. Testing method that been used were blotter test and agar test. Thirty genera of fungi were found from this experiment. They were Rhizopus, Fusarium, Mucor, Alternaria, Drechslera, Pythium, Botrytis, Penicillium, Aspergillus, Cercospora, Cercosporella, Trichoderma, Colletotrichum.Keywords : identification, fungi, paddy, seed-borne
RESPON REGENERASI BEBERAPA GENOTIPE DAN STUDI TRANSFORMASI GENETIK TOMAT (Lycopersicon esculentum Mill.) MELALUI VEKTOR Agrobacterium tumefaciens
ABSTRACTThe development of an effective Agrobacterium transformation for tomato depends on several factors including plant genotype, explant vigor, Agrobacterium strain, selection system and culture conditions. It has been reported that tomato genotype contributed to success of transformation and regenerability in tissue culture. The purpose of this experiment was to study of regeneration response of four tomato genotypes and genetic transformation of tomato through Agrobacterium tumefaciens vector. The experiment of tomato genotype regeneration study was conducted at Biotechnology Laboratory, Asian Vegetables Research and Development Center (AVRDC), Taiwan, meanwhile the experiment of tomato genetic transformation study was conducted at Molecular Biology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Bogor. Three of well-adapted genotypes in Indonesia i.e. Intan, Gondol Hijau and CL6046 were used as regeneration response experimental materials. AVRDC’s genotype, CLN1559A, was used as a control genotype in in-vitro culture. The result of experiment showed that there was variation response on the ability of explant to regenerate and form the shoot among the four genotypes. The control genotype, CLN1559, was the most regenerable genotype that indicated by the highest number of regenerated explants and shoots per explants (90,56% and 0,66), followed respectively by Intan (66,22% and 0,37), CLN6046 (45,94% and 0,26) and Gondol Hijau (17,08% and 0,09). Based on the results, among of the well-adapted genotypes in Indonesia, Intan was the most responsive genotype to be regenerated compared with two other genotypes. Considerably, Intan could be used as an explant for study on genetic engineering tomato through A. tumefaciens vector. The results of genetic transformation study have successfully obtained several explants formed the callus dan shoot on medium selection containing 50 mg/l kanamycin. Nevertheless, transformaton efficiency in the experiment still low which of 55,7 explants in average generated 1,7 shoots (about of 3%) that survive on selection medium (50 mg/l kanamycin). Keywords: Lycopersicon esculentum Mill., Regeneration, Genetic Transformation, Agrobacterium tumefaciens
PENGARUH PENGHEMATAN PUPUK BUATAN MELALUI PENGGUNAAN PUPUK ORGANIK TERHADAP PRODUKTIVITAS PADI (Oryza sativa L.) PADA SISTEM TANAM SRI
ABSTRACT The study of influence organic manure to save the fertilizer to get yield of rice (Oriza sativa L.) at system plant SRI have been conducted at paddy soil at Manggindo Jorong Sangkir Kenagarian Lubuk Basung Province West Sumatra in the year 2009. Target was to get measuring fertilize able to be economized by using organic manure to increase result of rice field paddy in conducting of SRI. Attempt used Complete Random Design (RAL), with treatment of comparison of thrift of usage of fertilizer with addition of organic manure 6 treatment and 4 times restating, so that there were 24 attempt plot. As for fertilizer and organic manure which used in this research [is] : 200 kg/ ha Urea, 100 kg / ha SP-36, 50 kg / ha MOP and 10.000 Sink / ha Compost hay + cage manure ( v / v). The treatment [is] : 100% Manure Made in without organic manure ( A); 100% manure made in + 100% organic manure ( B); 75% manure made in + 100% organic manure ( C); 50% manure made in + 100% organic manure ( D); 25% manure made in + 100% organic manure ( E); and 100% organic manure without manure made in ( F). Data of Disidikragam, if F [count/calculate] bigger than real F level 5% continued with Test of Duncan New Method of Range Test ( DNMRT) [at] real level 5% by using program of SPSS 15. Perception for example is a. High [of] crop, Amount of anakan per clump, Amount of productive anakan per clump, Amount of productive anakan, length of Malai, Amount of shell of rice per malai,. Amount of shell of rice of bernas per malai, wight 1000 seed, dry Production shell of rice per plot and per hectare. Best result obtained from treatment with [gift/ giving] of manure made in 100% with organic manure 100% with production rate of gabak dry equal to 5, 05 ton / Ha. Boundary thrift of usage of manure made in with addition of organic manure able to be lenient by paddy of IR42 to obtain;get result of maximal [at] this research [is] treatment that is 25% manure made in + 100% organic manure. Key words: SRI, organic manure, saving fertilize
PERTUMBUHAN DAN HASIL TANAMAN WIJEN DENGAN PEMBERIAN FUNGI MIKORIZA ARBUSKULA (FMA) DAN BOKASHI TITHONIA PADA TANAH ULTISOL
ABSTRACTThis research aims to obtain dose combination bokashi Tithonia and more precise AMF on growth and yield of sesame in the Ultisol. This research used Completely Randomized Design with 3 replications. The treatment is given, the first factor (A) is a measure bokashi Tithonia 4 levels namely, 0, 1, 2, and 3 t ha-1. The second factor (B) is 3 levels of arbuscular mycorrhizal fungus (AMF), namely: 25, 50 and 75 g plant-1. Investigated range of advanced real test, if the F count > F Table 5% with advanced test LSD 5% level. The results showed that bokashi tithonia and mycorrhizal provides no real interaction, but a single real influence. Giving bokashi Tithonia 3 t ha-1 and FMA 50 g plant-1 gives growth and yield of sesame is the best on Ultisol.Key words: AMF, Tithonia, Sesamum