Jurnal Agroekoteknologi
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MORFOGENESIS ANGGREK (Anoectochilus formosanus) SECARA IN VITRO
Full text linkABSTRACTAnoectochilus formosanus is one species of Orchids, known as a “Jewel Orchids”and have been used as a folk medicine in China and Taiwan.The aim of this studywas to examine the response of using different types of explants and combination of growth regulators TDZ and BAP to morphogenesis of Anoectochilus formosanus Orchids by in vitro. The results showed that using different types of explants had very significant effect to the percentage of callus and shoot morphogenesis on 12 weeks after planting, the number of adventitious buds, adventitious shoot length, and callus diameter on three weeks until 12 weeks after planting. The best explant to callus morphogenesis was showing on shoot tip explant (50%) and to shoot morphogenesis was showing on auxillary buds and internode explant (100%). The best number of adventitious buds was observed in second internode explant with average number of shoots are 7.62 shoots. The best adventititous length was observed in axillary buds explant with average number of shoot length in 1.16 cm, and the best callus diameter was observed in shoot tip explant with average of diameter in 2.2 mm. The combination of plant growth regulator TDZ and BAP had a very significant effect to adventitious length on 10 weeks after planting, and a significant effect to adventitious length on 11 and 12 weeks after planting. The best adventitious length was observed on 0.25 mg L-1 TDZ + 0.75 mg L-1 BAP in 1.13 cm per explant on 12 weeks after planting. The best combination to callus and shoot morphogenesis was observed on 0.75 mg L-1 TDZ + 0.25 mg L-1 BAP. There was no interaction between used a different types of explant and combination plant growth regulator to morphogenesis Anoectochilus formosanus orchids by in vitro
POTENSI MIKROBA ASAL MIKROORGANIME LOKAL DALAM MENINGKATKAN PERKECAMBAHAN BENIH PAPRIKA
Full text linkABSTRACTThis experiment was continues from the study of biological fertilizers potential’slocal microorganisms from the best main raw materials obtained from previousexperiments. This experiment used five selected raw materials to make localmicroorganisms, including: bamboo roots, broccoli leaves, chicken manure, rabbit manure and goat manure, each raw material was made 2 times so that there were 10 local microorganisms samples. The results showed that the highest of total bacterial population were shown by local microorganisms goat manure with a value of 7.2 x 105 cfu/ml, then the highest population of Azotobacter and Azospirillium were shown by local microorganisms rabbit manure with values of 6.4 x 104 cfu/ml and 3.5 x 103 cfu/ml and total fungi populations was local microorganisms goat manure with a value of 4.7 x 103 propagules/ml. In the pathogenicity test, 9 nine bacterial isolates were obtained, 25 Azotobacter isolates, 15 Azospirillium isolates were proven to provide negative responses to the pathogenicity test. Furthermore, in the seed nurserytest, only 33 microbial isolates were obtained which could support the growth ofpaprika seeds with the best results were shown by aquadest water control, 4B-1 NFB, 5A-1 NFB, and 1A-2 NFB isolates respectively.
PENGARUH KONSENTRASI GIBERELIN TERHADAP PERTUMBUHAN DAN HASIL BEBERAPA VARIETAS PADI SAWAH PADA CEKAMAN SALINITAS TINGGI
Full text linkABSTRACTThis study aimed to: (1) study the effect of interactions between gibberellin concentration and several wetland rice varieties on conditions of high salinity stress on growth and yield, (2) gibberellin concentration and lowland rice varieties which have the best influence on growth and the results of lowland rice. The research was carried out at the screen house of the Faculty of Agriculture, Winaya Mukti Tanjungsari University - Sumedang Regency with altitude 850 m above sea level. The time of the study was carried out in December 2014 until April 2015. The study used a factorial Randomized Block Design method, which consisted of two factors: gibberellin concentration and several varieties of lowland rice in high salinity stress. The first factor is the concentration of gibberellin (G), consisting of four levels: g0 = soaking the seeds for 24 hours in giberelin 0 mgL-1 = soaking the seeds for 24 hours in gibberellins 20 mgL-1, g2 = soaking the seeds during 24 hours in gibberellins 40 mgL-1, g3 = soaking the seeds for 24 hours in giberelin 60 mgL-1. The second factor is rice paddy variety (P), consisting of eight levels: p1 = Inpari 10, p2 = Inpari 20, p3 = Inpari 25, p4 = Inpari 26, p5 = Inpari 27, p6 = Inpari 30, p7 = Inpara 4, and p8 = Banyuasin. The results of the study indicate that here is an interaction effect between gibberellin concentrations and some lowland rice varieties in high salinity tresses against root drop ratio at ages 7, 35 and 49 HST. Rice varieties independently affect the age of harvest and the weight of 1000 seeds. There is a concentration of gibberellins and lowland rice varieties that produce the best growth and yield in high salinity stress
RESPONS PERTUMBUHAN TANAMAN HIAS ANTHURIUM ‘GELOMBANG CINTA’ (Anthurium plowmanii) PADA BEBERAPA KONSENTRASI BAP DAN FREKUENSI PEMBERIAN PUPUK DAUN
Full text linkOrnamental plants anthurium wave love great demand of society because the leaves are beautiful, unique and interesting. The edges of the leaves of anthurium generally with characteristic wavy. Anthurium plant growth stimulation can be enhanced by the application of growth regulators BAP and foliar fertilizers. The purpose of this study was to obtain a concentration of growth regulators BAP and best foliar fertilizer for growing ornamental plants anthurium. Experiments was carried out in the plant tissue culture laboratory of the Faculty of Agriculture, Andalas University from November 2015 to February 2016. Materials used in the love wave anthurium seeds, plant media are a mixture of soil, sand, compost 1: 1: 1 (v / v). Two-factor factorial experiment arranged in completely randomized design with three replications. The first factor was the concentration of BAP (0, 2.5, 5.0 and 7.5 mg/L) and the second factor was the frequency of giving leaf fertilizer (0, 1, 2 times). The variables observed were plant height, leaf width, root length and fresh weight of plants. The data were analyzed by analysis of variance followed by Honestly Significant Difference test 5%. The results showed that there is an interaction between BAP and the frequency of leaf fertilizer application to plant height and root length. The best BAP 2.50 mg/L for leaf width growth and 5.0 mg/L best to increase the fresh weight of plants. The best BAP concentration of 2.50 mg/L for leaf width growth and 5.0 mg/L is best for increasing the fresh weight of plants. Application of leaf fertilizer with a frequency of 2 times shows the highest plant heightKeywords: Anthurium, BAP, Concentration, Foliar fertilizer, Frequenc
INTRODUKSI FORMULA RIZOBAKTERIA Bacillusthuringiensis pv. toumanoffi PADA TANAMAN KEDELAI UNTUK PENINGKATAN KETAHANAN TERHADAP PENYAKIT PUSTUL BAKTERI (Xanthomonas axonopodispvglycines) DI LAPANGAN
Full text linkBacillus thuringiensis srv.toumanoffi is an indigenous endophytic rizobacteria from healthy soybean rizosphere capable of controlling bacterial pustule disease (Xanthomonas axonopodispvglycines). Rhizobacteria needs to be formulated to remain effective in storage and application. The aimed of this research was to obtain a stable bacterial formula in controlling bacterial pustule disease in soybeans. This research was designed in factorial in a randomized group consisting of 13 treatments with 3 replications. The Treatment was a combination of carrier material (peat, tapioca and coconut water+1% palm oil) and storage time (0, 2, 4, and 6 weeks). Soybean seeds were introduced with each Bacillus thuringiensis srv. toumanoffi formula before planting. The results showed that the formula Bacillus thuringiensissrv. toumanofi was relatively stable in suppressing the incidence and severity of bacterial pustule disease in soybean leaf. The best formula for controlling bacterial pustules was Bacillus thuringiensis srv. toumanoffi in peat, coconut water + palm oil and tapioca each 2 weeks storage
RESPON TANAMAN CABAI MERAH (Capsicum annum L.) YANG DIAPLIKASIKAN BAKTERI ENDOFITIK DIAZOTROF DAN PUPUK N TERHADAP POPULASI ENDOFITIK DIAZOTROF, KONSENTRASI N, DAN BOBOT KERING TANAMAN PADA INCEPTISOLS JATINANGOR
Full text linkThe aim of the research was to determine the effect of nitrogen-fixing endophytic bacteria and N fertilizer on population of nitrogen-fixing (diazotroph) endophytic bacteria, N concentration and dry matter of red chili plant (Capsicum annuum L.) on Inceptisols from Jatinangor. The experiment conducted design was Randomized Block Design in factorial pattern consisted of two factor and three replication. The first factor was diazotroph endophytic bacteriaconsisted two levels: without used diazotroph endophytic bacteria, and used diazotroph endophytic bacteria. The second factor was dosage of N anorganic fertilizer consisted four levels 0, 50, 100 and 150 kg N ha-1. The results showed that there is interaction found between diazotroph endophytic bacteria and N inorganic fertilizer on population of diazotroph endhophytic bacteria but there were not interaction effect on N concentration and dry matter of red chili plant. Independenly, treatment giving diazotroph endophytic bacteria and N anorganic fertilizer showed not significant effect on N concentration of plant. While the treatment of diazotroph endophytic bacteria and 150 kg N ha-1 inorganic fertilizer were independenly each could significantly increased dry matter of red chili plant on Inceptisols from Jatinangor. Keywords: Diazotroph endophytic bacteria, N fertilizer, Red chili plan
PENINGKATAN VIABILITAS DAN VIGOR BENIH PADI SAWAH (Oryza sativa L.) DENGAN TEKNIK BIOMATRICONDITIONING
Full text linkThis research was aimed to examine and get biomatriconditioning technique that increase viability and seed vigor rice (Oryza sativaL.). This research was conducted at Agronomi Unit of the Laboratory Agrotechnology of Haluoleo University, Kendari from January until March 2013. This research used completely randomized design with factorial. The first factor was the variety: Konawe and Inpari 10, the second factor was biomatriconditioningtechnique Control, Bacillus CKD061+Husk Charcoal Powder, Bacillus CKD061+Powder Red Brick. Serratia CMN 175 + Husk Charcoal Powder, Serratia CMN175+Red Brick powder. For the all numbers there were 14 combination treatments, repeated for 3 times, so the total of experimental units were 42. Replication consisted of 5 polybag so there were 210 plants. Data were analyzed using a manner sidik, followed by Test Honestly Significant Difference. The results showed that the best biomatriconditioning for Konawe varieties was Serratia CMN175 + rice husk powder treatment, while for the Inpari varieties 10 Bacillus CKD061 + husk powder for the both of treatment could be able to improve simultaneity grow, vigor index, relative growth speeds, T50, plant height and number of tillers.Keywords: Bacillus CKD061, P. fluorescensPG01, seed of rice, Serrati
PENGARUH 2iP DAN AIR KELAPA TERHADAP MULTIPLIKASI TUNAS BAWANG MERAH (Allium Ascalonicum L.) KULTIVAR SUMENEP SECARA IN VITRO
Full text linkGenerative propagation of onion sumenep cultivars had not provided optimum results. To overcome this problem vegetative propagation was used with tissue culture systems. Growth Regulator (ZPT) 2iP and Coconut Water were used as a source of cytokines to stimulate explant. This research was aimed to observe effect of 2iP and coconut water in stimulating red onion compound shoots. This experiment was carried out using a completely randomized design consisting of two factors. The first factor was the provision of 2iP with five levels of concentration of 0.0, 2.0, 4.0, 6.0, 8.0 mg/l and the second factor was the provision of coconut water with three levels of concentration of 10, 20 and 30%. The parameters observed were shoot number, leaf number, leaf length, root number and root length .The results of this research showed that the combination of 8.0 mg/l 2iP and 20% coconut water resulted in an average number of shoots and the highest number of leaves, each of which was 6.5 shoots and 6.3 leaves at 8 MSP. The combination of 2iP treatment and coconut water gave more significant effect than that of the single treatment
EFEKTIVITAS HARA MAKRO DAN MIKRO TERHADAP PERTUMBUHAN TANAMAN JAGUNG (Zea mays L.)
Full text linkNutrient absorption in plants must be complex. Plants were able to produce optimum productivity, if the land grew the plant had a good fertility rate. The elements were most needed by plants to complete their life cycles were elements of N, P, and K. The purpose of this research was to estimate soil fertility to biological nutrition. The research was conducted in September until November 2017 at Green House of STIPER Petra Baliem Wamena, Papua. The treatments were assigned in Randomized Complete Design with a combination of fertilization, namely:Control (Without Treatment (K0), without N (PK), without P (NK), without K (NP), and complete fertilizer (NPK). The data was analyzed by using Anova with α = 5%, then followed by DMRT α 5%. The results showed that NK treatment gave a good effect for plant growth especially on plant height and ratio of plant leaf area. While, NP treatment gave a good influence to plant physiological properties, such as plant chlorophyll content and plant rate of assimilation.Keywords: effectiveness, hara, mayz
HUBUNGAN LUAS DAUN TERHADAP PERTAMBAHAN BERAT KERING RUMPUT GAJAH DI BERBAGAI PERLAKUAN DI MEDIA TAILING TIMAH
Full text linkElephant grass (Pannisetum purpureum) can grow in various types of land conditions and produces and is resistant to environmental stress. The predominant sand tin tailing conditions and poor nutrients cause limited plant growth above them. This research tested some kind of fertilizer treatment on sand tailing media after tin addition by using elephant grass plants. The results showed that the type of fertilizer had significant effect on the specific leaf area variant, the ratio of leaf area and the leaf weight ratio. Overall growth were greatly depressed on the 100% tailings treatment. Lime applied in conjunction with NPK fertilizer was not show the total leaf weight that was different from the addition of NPK alone. NPK fertilizers better influence the growth of elephant leaves in tin tailings than organic fertilizers. The best treatment was obtained on organic fertilization with NPK inorganic fertilization. The association of total dry weight of elephant grass with specific area of elephant grass leaf was only visible in organic fertilization treatment together with NPK inorganic fertilizer. In the best condition of elephant grass growth, the specific leaf area had a significant role to increase the total weight of the plant with the value of r2 = 0.864 or LDS will affect the total dry weight of elephant grass in the tailings medium with the addition of organic and inorganic materials NPK of 86.4 percent the remaining 13.6% increase in total dry weight was influenced by factors other than specific leaf area