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Good luck, elementary school students. Support future doctors! - Report on the activities of the Mizuta Memorial Library Student Advisers -
Get PDF地域連携報告 / Reports 編集：城西大学・城西短期大学地域連携センター紀要編集委員
Collaborative development of bread by training course students of registered dietitian - Focusing on ingredients produced in Saitama Prefecture -
Get PDF地域活動ノート / Notes 編集：城西大学・城西短期大学地域連携センター紀要編集委員
エイユウ ショウハシ ハジマリ ノ トウイツ オウ ポスター
Get PDF開催日：2024年1月20日（土）～2月21日（水）開館時間：午前9時30分～午後4時30分 休館日＝土曜日、日曜日、祝日、1月23日（火）、2月5日（月）※1月20日（土）、21日（日）は開館します。観覧料 ： 無料 会場 ： 城西大学水田美術館ギャラリー1 企画・監修：山本 正昭（沖縄県立博物館 ・ 美術館 主任学芸員）協力：沖縄県立博物館・美術館 後援：沖縄県立博物館友の会
Phospholipid Scramblase 1 Localized Proximal to Sphingomyelin Synthase Isoforms but s Not Involved in Sphingomyelin Synthesis
Get PDFCeramide (Cer) is synthesized de novo in the bilayer of the endoplasmic reticulum and transported to the cytosolic leaflet of the trans-Golgi apparatus for sphingomyelin (SM) synthesis. As the active site of SM synthase (SMS) is located on the luminal side of the Golgi membrane, Cer translocates to the lumen via transbilayer movement for SM synthesis. However, the mechanism of transbilayer movement is not fully understood. As the Cer-related translocases seem to localize near the SMS, the protein was identified using proximity-dependent biotin identification proteomics. Phospholipid scramblase 1 (PLSCR1), which is thought to act as a scramblase for phosphatidylserine and phosphatidylethanolamine, was identified as a protein proximal to the SMS isoforms SMS1 and SMS2. Although five isoforms of PLSCR have been reported in humans, only PLSCR1, PLSCR3, and PLSCR4 are expressed in HEK293T cells. Confocal microscopic analysis showed that PLSCR1 and PLSCR4 partially co-localized with p230, a trans-Golgi network marker, where SMS isoforms are localized. We established CRISPR/Cas9–mediated PLSCR1, PLSCR3, and PLSCR4 single-knockout cells and PLSCR1, 3, 4 triple knockout HEK293T cells. Liquid chromatography-tandem mass spectrometry revealed that the levels of species with distinct acyl chains in Cer and SM were not significantly different in single knockout cells or in the triple knockout cells compared to the wild-type cells. Our findings suggest that PLSCR1 is localized in the vicinity of SMS isoforms, however is not involved in the transbilayer movement of Cer for SM synthesis.This is an open access article distributed under the terms of Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/). Published by The Pharmaceutical Society of Japa
Smile up caffe オゴセマチ バイリン カフェ デ ノ ワンデイ カフェ キカク ウンエイ ノ トリクミ
Get PDF2024年度運営メンバー 教員：君羅好史、小暮更紗、中里見真紀、佐藤景子、大学院生：３名 ３年生：４名 ２年生：１０名 １年生：９名（学生合計２６名
Galectin-2 Agglutinates Helicobacter pylori via Lipopolysaccharide Containing H Type I Under Weakly Acidic Conditions
Get PDFGalectins are β-galactoside-binding animal lectins involved in various biological functions, such as host defense. Galectin-2 and -3 are members of the galectin family that are expressed in the stomach, including the gastric mucosa and surface mucous cells. Galectin-3 exhibits aggregation and bactericidal activity against Helicobacter pylori in a β-galactoside-dependent manner. We previously reported that galectin-2 has the same activity under neutral pH conditions. In this study, the H. pylori aggregation activity of galectin-2 was examined under weakly acidic conditions, in which H. pylori survived. Galectin-2 agglutinated H. pylori even at pH 6.0, but not at pH 5.0, correlating with its structural stability, as determined using circular dichroism. Additionally, galectin-2 binding to the lipopolysaccharide (LPS) of H. pylori cultured under weakly acidic conditions was investigated using affinity chromatography and Western blotting. Galectin-2 could bind to H. pylori LPS containing H type I, a Lewis antigen, in a β-galactoside-dependent manner. In contrast, galectin-3 was structurally more stable than galectin-2 under acidic conditions and bound to H. pylori LPS containing H type I and Lewis X. In conclusion, galectin-2 and -3 might function cooperatively in the defense against H. pylori in the stomach under different pH conditions.13p. Article number: 8725, Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
キタサカド ニギワイ サロン ツウシン ダイ107ゴウ
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