Acta VETERINARIA Indonesiana
Not a member yet
324 research outputs found
Sort by
Identification of Herpesviridae in Macaca Fascicularis Using the Nested PCR Method at PSSP IPB
Get PDFPCR technique is used to detect the presence of Herpesviridae viruses in Macaca fascicularis that exhibit no clinical symptoms and identify them using nucleotide base sequences. This study aimed to detect and identify the presence of nucleotide base sequences in the Herpesviridae virus family in Macaca fascicularis at PSSP IPB. The study utilized 20 nasal swab samples from Macaca fascicularis. Extraction of DNA utilizing the QiaAmp™ DNA mini kit and subsequent measurement of DNA concentration. Amplification of target DNA utilizing the nested PCR technique. Sample with positive results from electrophoresis were sequenced to obtain nucleotide sequences. The average measurement value of DNA extraction concentration was 25.07 ng/µl. The DNA purity ratios at wavelengths A260/A280 and A260/A230 averaged 1.95 and 0.27, respectively. The electrophoresis results indicated a band size of 215 bp in sample code 01, corresponding to the positive control band. The sequencing results were analyzed using BLASTn on the NCBI site, revealing similarity with Macaca fascicularis lymphocryptovirus. The BLAST sample results demonstrated a Query Cover value of 100% and a percentage of identity of 98.84%.PCR technique is used to detect the presence of Herpesviridae viruses in Macaca fascicularis that exhibit no clinical symptoms and identify them using nucleotide base sequences. This study aimed to detect and identify the presence of nucleotide base sequences in the Herpesviridae virus family in Macaca fascicularis at PSSP IPB. The study utilized 20 nasal swab samples from Macaca fascicularis. Extraction of DNA utilizing the QiaAmp™ DNA mini kit and subsequent measurement of DNA concentration. Amplification of target DNA utilizing the nested PCR technique. Sample with positive results from electrophoresis were sequenced to obtain nucleotide sequences. The average measurement value of DNA extraction concentration was 25.07 ng/µl. The DNA purity ratios at wavelengths A260/A280 and A260/A230 averaged 1.95 and 0.27, respectively. The electrophoresis results indicated a band size of 215 bp in sample code 01, corresponding to the positive control band. The sequencing results were analyzed using BLASTn on the NCBI site, revealing similarity with Macaca fascicularis lymphocryptovirus. The BLAST sample results demonstrated a Query Cover value of 100% and a percentage of identity of 98.84%
Domestic Pigs as an Animal Model of Myocardial Infarction: a Comparison of Systolic and Diastolic Blood Flow Velocities of Cardiac Valves
Get PDFCardiovascular disease (CVD) is still the leading cause of death according to WHO in 2021. CVD generally results in myocardial infarction (MI), which occurred after tissue ischemia. Echocardiography is most likely a diagnostic tool without invasive effects. This study aimed to compare the blood flow in pig research animals treated with ligases of the circumflex arteries. Measurements were made in the mitral valve (MV), tricuspid valve (TV), semilunar pulmonary valve (SLP), and semilunar aortic valve (AO) of velocity during systole (Vs), velocity during diastole (Vd), time average peak velocity (TAMAX), and blood flow pressure at three-time points: before ligase treatment, after acute infarction (60 min), and 8 weeks after ligase treatment. All parameters in MV group increased at 60 min and decreased at 8th weeks. On TV, TAMAX and pTAMAX decreased at 60 min and week 8. In SLP, VS, pVS, and Vd decreased at 60 min and increased at 8th week. The pVd and pTAMAX parameters decreased at 60 min and 8th week, while TAMAX increased at 60 min and decreased at 8th week. In AO, the TAMAX and pTAMAX parameters increased at 60 min and 8 weeks; the other parameters were similar to MV and TV patterns.Cardiovascular disease (CVD) is still the leading cause of death according to WHO in 2021. CVD generally results in myocardial infarction (MI), which occurred after tissue ischemia. Echocardiography is most likely a diagnostic tool without invasive effects. This study aimed to compare the blood flow in pig research animals treated with ligases of the circumflex arteries. Measurements were made in the mitral valve (MV), tricuspid valve (TV), semilunar pulmonary valve (SLP), and semilunar aortic valve (AO) of velocity during systole (Vs), velocity during diastole (Vd), time average peak velocity (TAMAX), and blood flow pressure at three-time points: before ligase treatment, after acute infarction (60 min), and 8 weeks after ligase treatment. All parameters in MV group increased at 60 min and decreased at 8th weeks. On TV, TAMAX and pTAMAX decreased at 60 min and week 8. In SLP, VS, pVS, and Vd decreased at 60 min and increased at 8th week. The pVd and pTAMAX parameters decreased at 60 min and 8th week, while TAMAX increased at 60 min and decreased at 8th week. In AO, the TAMAX and pTAMAX parameters increased at 60 min and 8 weeks; the other parameters were similar to MV and TV patterns
A Pengamatan Kadar BUN dan Kreatinin Pada Kucing Yang Mengalami FLUTD (Feline Lower Urinary Tract Disease)
No full textPenelitian ini bertujuan untuk mengetahui nilai Blood Urea Nitrogen (BUN) dan kreatinin pada kucing penderita Feline Urinary Tract Disease (FLUTD). Kucing dengan FLUTD seringkali mengalami gangguan ginjal seperti azotemia dan kelainan ginjal kronis yang disebut Chronic Kidney Disease (CKD) . Kedua kondisi tersebut terlihat dari tingginya kadar BUN dan Kreatinin dalam darah. Kadar BUN dan Kreatinin diperiksa dengan mengambil serum darah kucing penderita FLUTD menggunakan spuit sekali pakai berukuran 3 cc dan ditampung dalam tabung kemudian disentrifugasi dengan kecepatan 3000 rpm selama 10 menit. Alat yang digunakan untuk melakukan pemeriksaan BUN dan kreatinin adalah alat pemeriksaan kimia darah VetTest Idexx Laboratory®. Hasil pemeriksaan pada 25 ekor kucing terdapat 64% (16/25) mengalami peningkatan nilai BUN dan 36% (25/9) mengalami peningkatan nilai kreatinin. Berdasarkan hasil penelitian, dapat disimpulkan bahwa kucing dengan FLUTD juga akan mengalami gangguan ginjal, sehingga diperlukan terapi tambahan untuk menurunkan kadar BUN dan kreatinin.Feline Lower Urinary Tract Disease (FLUTD) is one of the most common diseases affecting cats. One type of FLUTDthat often occurs is urinary tract obstruction. Urinary tract obstruction makes it difficult for cats to urinate, causing urea and creatinine to be reabsorbed into the bloodstream. This study aimed to examine of Blood Urea Nitrogen (BUN) and creatinine values in cats with Feline Urinary Tract Disease (FLUTD). Cats with FLUTD often had kidney problems such as azotemia and chronic kidney disorders called Chronic Kidney Disease (CKD). Both conditions can be seen from high levels of BUN and Creatinine in the blood. The BUN and Creatinine levels were examined by taking blood serum from cats with FLUTD using a 3 cc disposable syringe and accommodated in a plain tube and then centrifuged at 3000 rpm for 10 minutes. The tool used to perform BUN and creatinine examinations is the VetTest Idexx Laboratory® blood chemistry examination tool. The result of the examination on 25 cats was 64% (16/25) had an increase in BUN values and 36% (9/25) had an increase in creatinine values. BUN and creatinine are waste products of metabolism that are filtered by the glomerulus and excreted in the urine. Elevated levels of BUN and creatinine in cats with FLUTD are due to the cat\u27s inability to urinate as a result of urinary tract obstruction or decreased glomerular filtration rate. Based on the results, it can be concluded that cats with FLUTD will also have kidney problems, and additional therapy is needed to reduce BUN and creatinine levels
Anatomical and Histological Features in Muscles of Wild Boar (Sus scrofa) from Timor Island
Get PDFThe anatomical features and histology of wild boars serve as two parameters for assessing the quality of wild boar meat. This study aimed to identify the anatomical and histological characteristics of wild boar muscles (Sus scrofa) from Timor Island, with potential benefits for the community and veterinary students. The research samples were the longissimus dorsi and biceps femoris muscles from three wild boars slaughtered in the laboratory. Muscle samples were prepared for anatomical observations. Muscle samples were anatomically observed, fixed in 10% alcohol, and then prepared for histological analysis using Hematoxylin and Eosin (HE) staining. The anatomical observation indicated that the boar\u27s muscles exhibited a blackish-red coloration. The longissimus dorsi muscles had a chewy consistency, while the biceps femoris muscles were also chewy, displaying slight density and the distinctive aroma of fresh meat. Histological images indicated that muscle fibers appeared polygonal in shape in transverse sections, while elongated in longitudinal sections. Some fasciculi composed of muscle fibers exhibited multiple nuclei located at their peripheries. Between the muscle fibers, round fat cells with a nucleus were surrounded by three types of connective tissue. The histological characteristics of wild boar exhibit similarities to those of the Timor pig, Sumatran Ongole cattle, and Bali cattle.Gambaran anatomi dan histologi babi hutan merupakan dua parameter yang dapat digunakan untuk menentukan kualitas dari daging babi hutan. Tujuan dari penelitian ini adalah untuk mengetahui gambaran anatomi dan histologi otot babi hutan (Sus scrofa) asal Pulau Timor yang dapat bermanfaat bagi masyarakat dan mahasiswa kedokteran hewan. Sampel penelitian yang digunakan adalah otot longissimus dorsi dan bicep femoris dari tiga ekor babi hutan yang disembelih di Laboratorium. Sampel otot kemudian dilakukan pengamatan anatomi. Sampel otot yang telah diamati secara anatomi, kemudian dilakukan proses fiksasi menggunakan alkohol 10% dan dilanjutkan pembuatan preparat histologi serta pewarnaan Hematoksilin Eosin (HE). Gambaran anatomi yang diamati adalah otot babi hutan berwarna merah kehitaman, konsistensi otot longissimus dorsi kenyal dan otot bicep femoris kenyal dengan sedikit padat dan bau khas daging segar. Gambaran histologi menunjukan pada potongan transversal serabut otot akan terlihat berbentuk poligonal dan pada potongan longitudinal serabut otot akan terlihat memanjang. Terdapat beberapa fasikulus yang dibentuk oleh serabut otot juga memiliki inti banyak di tepinya. Terdapat sel lemak diantara serabut otot yang berbentuk bulat, inti sel lemak dan tiga jaringan ikat.
 
Daily Behavioral Patterns of Timor Deer (Rusa timorensis) Fawns at CV. Bahtera Satwa Breeding Center, Margorejo Village, Dawe District, Kudus Regency: Pola Perilaku Harian Anak Rusa Timor (Rusa timorensis)
No full textThe phenomenon of the declining population of Timor deer (Rusa timorensis) has led the Indonesian government to designate them as a protected species. Captive breeding has become an effort to conserve Timor deer, and daily behavior serves as a measure of the success of the breeding program. The objective of this research was to evaluate the daily behavior patterns of Timor deer fawns, including movement, feeding, resting, social, alert, eliminative, and grooming behavior at the CV. Bahtera Satwa Breeding Center. Data collection for the research was conducted over 14 days using the scan sampling method. The results showed significant differences (p<0.05) in movement behavior, feeding, resting, social, and eliminative behavior between morning, afternoon, and evening. The frequency of social behavior in deer fawn was observed more often than in other behaviors, while the duration of resting activities was higher compared to other activities. The conclusion of this study was that the daily behavior patterns of Timor deer fawns, consisting of social and resting behaviors, were consistently performed in the morning and afternoon. The social behavior exhibited by the fawns was an effort to interact and explore their environment, while resting behavior was used to avoid heat and recover energy after morning activities.Fenomena penurunan populasi rusa timor membuat pemerintah Indonesia menetapkan rusa timor sebagai jenis satwa yang dilindungi. Penangkaran menjadi upaya pelestarian rusa timor, perilaku harian menjadi tolak ukur keberhasilan penangkaran sebagai indikator tingkat stres pada satwa, khususnya anak rusa timor. Penelitian ini bertujuan mengevaluasi pola perilaku harian anak rusa timor yang meliputi perilaku berpindah, perilaku makan, perilaku istirahat, perilaku sosial, perilaku waspada, perilaku eliminatif, dan perilaku grooming pada pagi, siang dan sore hari di Penangkaran CV. Bahtera Satwa Dawe Kudus. Pengambilan data penelitian dilakukan selama 14 hari menggunakan metode scan sampling. Hasil penelitian menunjukkan terdapat perbedaan yang nyata (p<0,05) pada perilaku berpindah, perilaku makan, perilaku istirahat, perilaku sosial, dan perilaku eliminatif antara pagi, siang, dan sore hari. Frekuensi perilaku sosial anak rusa lebih sering ditemukan daripada perilaku lainnya, sedangkan durasi aktivitas istirahat lebih tinggi dibandingkan aktivitas lainnya. Kesimpulan dari penelitian ini adalah pola perilaku harian anak rusa timor berupa perilaku sosial dan istirahat konsisten dilakukan di pagi dan siang hari. Perilaku sosial yang dilakukan oleh anak rusa sebagai upaya untuk berinteraksi dan melakukan eksplorasi lingkungan, sementara perilaku istirahat dipergunakan untuk menghindari panas dan memulihkan energi setelah aktivitas pagi hari
Characterization and Supplementing of PVA-curcumin Nanofibers on the Initial Week Performance of Broiler Growth
Get PDFBroiler chickens are a vital global commodity, known for their rapid growth and efficiency, but their productivity has historically relied on Antibiotic Growth Promoters (AGP), necessitating the development of safe alternatives. This study aimed to assess the efficacy of Polyvinyl Alcohol (PVA)-curcumin nanofibers as a potential substitute for Antibiotic Growth Promoters (AGP) in improving the growth performance of broiler chickens. The nanofibers were synthesized via the electrospinning technique at varying PVA concentrations of 5%, 10%, and 15%, and their morphology was characterized by Scanning Electron Microscopy (SEM). The in vitro assay evaluated curcumin release profiles were measured in phosphate buffer (PBS, pH 7,4) at 37 °C. A seven-day in vivo experiment involving 30 broiler hens categorized into 5 groups to assess the impact of supplementation on body weight, feed intake, and feed conversion ratio (FCR). The synthesized PVA- curcumin nanofibers exhibited optimal dimensions (100–135 nm), with gradual curcumin release measuered at 43,5–51,5% at 5 minutes and 49,27–64,5% at 120 minutes. While statistical analysis showed no significant difference (p> 0,05). The treatment group showed higher average daily gain (ADG) (99,49–115,4 g) and feed intake (FI) (118,76–129,11 g), resulting in better feed conversion ratio (FCR) (0,921–1,00) compared to the control group. In conclusion, PVA-curcumin nanofibers were successfully synthesized, demonstrating their potential to improve growth performance, and are suitable as an alternative non-antibiotic growth promoter in broilers.Abstrak
Penelitian ini bertujuan untuk mengevaluasi efektivitas penggunaan nanofiber yang terbuat dari polivinil alkohol (PVA) dan kurkumin sebagai alternatif pengganti antibiotic growth promotor (AGP) untuk meningkatakn performa pertumbuhan ayam broiler. Metode: Nanofiber PVA-kurkumin disintesis menggunakan teknik electrospinning dengan variasi konsentrasi PVA, yaitu 5%, 10%, dan 15%. Morfologi serat diamati berupa struktur yang dianalisis dengan menggunakan scanning electron microscope (SEM), sedangkan pelepasan kurkumin diuji secara in vitro menggunakan larutan buffer fosfat saline (PBS) pH 7,4 pada suhu 37°C. Uji in vivo dilakukan selama 7 hari pada 30 ekor ayam broiler untuk menilai performa pertumbuhan. Hasil: Nanofiber PVA-kurkumin berhasil terbentuk dengan ukuran antara 100 hingga 135 nm. Selain itu, uji in vitro menunjukkan bahwa pelepasan kurkumin dilakukan secara bertahap atau terkontrol. Hasil uji in vivo menyatakan bahwa pengaplikasian nanofiber PVA-kurkumin mampu meningkatkan bobot badan, konsumsi pakan, dan feed conversion ratio (FCR) ayam broiler; meskipun demikian, analisis statistik tidak menunjukkan perbedaan yang signifikan antara kelompok perlakuan dan kontrol (p>0,05). Kesimpulan: Nanofiber PVA-kurkumin berpotensi dijadikan alternatif AGP untuk meningkatkan performa pertumbuhan ayam broiler.
Kata kunci: AGP, Ayam Broiler, Kurkumin, Nanofiber, Perform
A comparison of the Hematology Profiles from Preventive and Curative Tests on Lissachatina fulica Snail Mucin Cream for Atopic Dermatitis
Get PDFAtopic dermatitis (AD) is a chronic inflammatory skin disease. Continuous use of topical NSAIDs in AD cases has been shown to cause adverse effects. Lissachatina fulica mucin (LFM) cream, a natural alternative, has been used for wound healing and can be used as a preventative or curative agent in AD. The objective of this study is to assess the efficacy of LFM cream, exploring its potential as a preventative and curative measure in AD. A total of 36 male BALB/c mice were divided into three cream dosage groups: 0%, 5%, and 10%. The two treatments were preventive and curative, and the two testing times were 0 and 5 days, with three replicates each. Mice were induced with atopic dermatitis by applying 1% DNCB to a 1x1 cm area on their dorsal area for seven days. The preventive group used LFM cream for seven days before DNCB, while the curative group used it after DNCB. Blood samples were collected on days 0 and 5 of the treatment period and analyzed using a hematology analyzer. The data were analyzed using a two-way ANOVA with GraphPad Prism 10, followed by Tukey\u27s test at a 95% confidence level. The toxicity test confirmed that the LFM cream is non-toxic and safe for topical use. The DNCB compound 1% exhibited four main symptoms of atopic dermatitis: pruritus, erythema, excoriation, and lichenification. LFM cream was highly effective, significantly suppressing AD symptoms, with the ADSI score remaining below 2 (p<0.05). Specifically, the curative efficacy was demonstrated by a significant decrease in the ADSI score on day 5 (p<0.05). Hematological analysis revealed statistically significant differences (p<0.05) in the number of lymphocytes, neutrophils, and thrombocytes across groups. The LFM cream is effective and safe for preventative use and successfully treats acute AD at 5% concentration
Tes Identifikasi Cepat Prevalensi Tuberkulosis Zoonotik (Mycobacterium bovis) di Bandung Barat dan Pangalengan: Identifikasi Cepat Prevalensi Tuberkulosis Zoonotik
No full textTuberkulosis sapi (bTB) disebabkan oleh bakteri Mycobacterium bovis (M. bovis). Tuberkulosis sapi pada sapi perah diyakini berkontribusi terhadap peningkatan kasus tuberkulosis karena dapat menular ke manusia (zoonosis), sehingga disebut Tuberkulosis Zoonotik. Penularan melalui mulut dapat terjadi dengan mengonsumsi susu atau produk susu yang terkontaminasi bakteri. Tujuan penelitian ini adalah untuk mengidentifikasi prevalensi infeksi M. bovis sebagai bagian dari upaya mitigasi risiko penyakit bawaan makanan pada susu. Metode yang digunakan adalah bersifat deskriptif kualitatif, menggunakan pewarnaan AFB dan PCR dengan gen MPB70, RD1, CSB1, CSB2, dan CSB3 sebagai target amplifikasi DNA. Hasil menunjukkan bahwa prevalensi infeksi pada 60 sampel susu yang diuji dengan metode pewarnaan AFB adalah 0% (negatif), dan pada 6 sampel (10% dari semua sampel) yang diuji dengan PCR, prevalensi juga 0%. Simpulan penelitian ini adalah tidak ada M. bovis dalam sampel susu, menunjukkan risiko yang saat ini rendah, tetapi pemantauan berkelanjutan, diagnostik yang ditingkatkan, dan kolaborasi interdisipliner diperlukan untuk mengurangi penularan zoonotik dan dampak ekonomi. Upaya pencegahan dan pengendalian bTB di Indonesia berfokus pada promosi kesehatan seperti menghindari kontak dengan hewan terinfeksi, memasak daging secara menyeluruh, dan mengonsumsi susu olahanBovine tuberculosis is caused by the bacterium Mycobacterium bovis (M. bovis). Bovine tuberculosis in dairy cows is thought to contribute to an increase in tuberculosis cases because it can be transmitted to humans (zoonosis), hence the term Zoonotic Tuberculosis. Transmission through the mouth can occur by consuming milk or dairy products contaminated with bacteria. The objective of this study was to identify the prevalence of M. bovis infection as part of efforts to mitigate the risk of foodborne disease in milk. The study design was descriptive qualitative, using AFB staining and PCR with the MPB70, RD1, CSB1, CSB2, and CSB3 genes as DNA amplification targets. The results showed that the prevalence of infection in 60 milk samples tested using the AFB staining method was 0% (negative), and in 6 samples (10% of all samples) tested using PCR, the prevalence was also 0%. The conclusion is there is no M. bovis in milk, indicating a currently low risk, but ongoing surveillance, improved diagnostics, and interdisciplinary collaboration are needed to reduce zoonotic transmission and economic impacts. Efforts to prevent and control Bovine Tuberculosis in Indonesia focus on promoting community practices: avoiding contact with infected animals, cooking meat thoroughly, and consuming processed milk
Kriopreservasi Semen Kambing Peranakan Boer dengan Pengencer Andromed dan Ekstrak Daun Kelor
No full textThe nutrients contained in Moringa leaves can protect spermatozoa from damage during semen cryopreservation. This study investigated the effectiveness of Moringa leaf extract in Andromed extender on the quality of frozen semen from Boer crossbreed goats. Fresh semen was divided into four tubes in equal volume and diluted with Andromed (control), 98% Andromed + 2% Moringa leaf extract (ME-2), 96% Andromed + 4% Moringa leaf extract (ME-4), and 94% Andromed + 6% Moringa leaf extract (ME-6), respectively. Diluted semen was placed in a mini-straw (0.25 mL), equilibrated in a refrigerator at 5°C for 4 hours, and then frozen by positioning the straw 10 cm above the surface of liquid nitrogen for 15 minutes. Variables were evaluated after diluting and thawing, including percentage of spermatozoa motility, live, and intact plasma membrane (IPM). Results of this study showed that the percentage of spermatozoa motility, live, and IPM for ME-4 (56.25, 67.5, and 65.75%) were significantly (p<0.05) higher than control (47.5, 56.75, and 53%), ME-2 (51.25, 59.5, and 56.25%), and ME-6 (51.25, 62.25, and 56%). This study’s findings indicate that adding 4% Moringa leaf extract to Andromed extender is optimal for enhancing the quality of frozen semen in Boer crossbreed goats. ABSTRACT
Nutrien yang terkadung di dalam daun kelor dapat melindungi spermatozoa dari kerusakan selama proses kriopreservasi semen. Tujuan penelitian ini adalah menguji efektivitas ekstrak daun kelor di dalam pengencer Andromed terhadap kualitas semen beku kambing peranakan Boer. Semen segar dibagi ke dalam empat buah tabung reaksi dan masing-masing diencerkan dengan pengencer Andromed (kontrol), 98% Andromed + 2% ekstrak daun kelor (EK-2), 96% Andromed + 4% ekstrak daun kelor (EK-4), dan 94% Andromed + 6% ekstrak daun kelor (EK-6). Semen yang telah diencerkan dikemas di dalam straw mini (0,25 ml), dan diekuilibrasi di dalam refrigerator lemari es pada suhu 5oC selama 4 jam. Semen dibekukan dengan cara meletakkan straw 10 cm di atas permukaan nitrogen cair selama 15 menit. Variabel meliputi persentase spermatozoa motil, hidup, dan membran plasma utuh (MPU) dievaluasi setelah pengenceran dan thawing. Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa, hidup, dan MPU setelah thawing perlakuan EK-4 (56,25; 67,5; dan 65,75%) nyata (p<0,05) lebih tinggi dibandingkan dengan kontrol (47,5; 56,75; dan 53%), EK-2 (51,25; 59,5; dan 56,25%), dan EK-6 (51,25; 62,25; dan 56%). Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan 4% ekstrak daun kelor di dalam pengencer Andromed dapat meningkatkan kualitas semen beku kambing peranakan Boer.
 
Effectivity and Safety of Endo-1,4 -xylanase (Xylanase), Endo-1,3(4) beta-glucanase (Beta-glucanase), Endo-1,4 beta-glucanase (Cellulase), and α Amylase as A Growth Promoter in Broiler Chickens
Get PDFThis study evaluates the effectivity and safety of Endo-1,4 -xylanase (Xylanase), Endo-1,3(4) beta-glucanase (Beta-glucanase), Endo-1,4 beta-glucanase (Cellulase), and α Amylase that packed in Ronozyme-AX ct (MGHSPHYT+) as an alternative to antibiotic growth promoter. A total of 64 chickens were used, divided into four groups, with each group divided into two replicates, 8 chickens per replicate. The groups were divided into treatment group, and groups administered with the different dosage of supplementation, namely 184, 230, and 276 gram/ton feed for 28 days, consecutively. Parameters such as weekly weight gain, final weight, feed conversion ratio (FCR), and carcass weight were observed to determine growth performance. Meanwhile, parameters such as mortality, blood hematology and biochemistry were observed to determine the safety of the supplementation. Based on the results, there was a significant difference on weekly weight gain, particularly in the second week, and in the final weight, with the group administered with 184 gram/ton feed showing the highest result. Meanwhile, other parameters such as FCR and carcass weight did not show any significant difference. This may be due to the enzymes not working directly to increase feed utilization but work with improving nutrient digestibility and utilization in the body. The safety parameters showed that the supplementation was relatively safe as no significant difference was observed in mortality, blood constituents and biochemistry