Majalah Kedokteran Bandung
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Role of Surf Redfish (Actinopyga mauritiana) in Wound Incision Healing Activities of Diabetic Mice
Get PDFIt is estimated that 6% of the US population suffer from diabetes with 15% of them experience diabetic foot. Several studies have explored the various benefits of sea cucumber for human being, including its antioxidant nature. This study aimed to investigate the effect of surf redfish, one species of the sea cucumbers, in diabetic wound healing. This was an animal experimental study performed at the Pharmacology and Pharmaceutical laboratory of the Faculty of Pharmacy, Universitas Tjut Nyak Dhien Medan, Indonesia, during the period of February- July 2019. Twenty five mice in diabetic condition were divided into five groups: control, standard, ethanol extract of surf redfish-1, ethanol extract of surf redfish-2, and ethanol extract of surf redfish-3 3. The surf redfish was obtained from Lamreh Village in Aceh and the ethanol was extracted using the maceration method. The parameters used for evaluating the efficacy of surf redfish ethanol extract were blood glucose level, length of wound incision, and histopathological features. Surf redfish extract presented a significant reduction of blood glucose level which that followed the increase in the dose of the ethanol extract (p-value <0.05) where the most significant reduction was seen in the group with the highest dose on the last day. This trend was also seen for the length of the wound, where the reduction was significantly higher in treatment groups when compared to control (p-value <0.05). The histological study also confirmed the improvement in the clinical appearance of wound by showing significantly increased fibroblast cell number and collagen density (p-value <0.05). Overall, the surf redfish can help better incision's wound healing in diabetic condition
Use of Herb Combination (Jamu Atoke) Before Mating to Improve Rat Pup Health
Get PDFMortality and health disturbances in children often correlate with maternal health and fertility. Avocado, mung bean sprouts, and holy basil have been traditionally used to improve maternal health, before and during pregnancy. This study was aimed to assess the efficacy of herbal combination of avocado, mung bean sprouts, and holy basil (Jamu ATOKE) in optimizing reproductive health. Eighteen female Sprague Dawley rats (9–10 weeks old, BW: 180–250 g) were randomly divided into 3 treatment groups (n=6 rats for each group) of control, group I and II. ATOKE were added into rats drinking water and consumed for 30 days before pregnancy. After the rats were pregnant and gave birth, pup per parent ratio, pup mortality, and pup health performance (body weight gain, feed and drink consumption, motor activity, pup speed in finding light and feed, red blood cell (RBC) count, white blood cell (WBC) count and differential, SGPT, SGOT, blood urea nitrogen (BUN), creatinine, and proinflammatory mediators (TNF-α and IL-6) were analyzed. Pups born to in group I had a generally better health performance compared to the control group. There was a 100% mortality in the litter of group II due to the excessive aggressive behavior of the dams as a sign of toxicity. Administration of 2.5% Jamu ATOKE showed the best effects on the health and intelligence of the rat pup
Correlation between Albumin, Colon Leakage Score (CLS), and Anastomotic Leakage after Colorectal Cancer Resection
Get PDFAnastomotic leakage is still the most undesirable complication of colorectal surgery. The reported incidence of anastomotic leakage after colorectal surgery varies from 1.8% to 15.9%. The impact of anastomotic leakage is increased morbidity, mortality (12–30%), length of stay, and cost of hospitalization; hence, this complication should be prevented as much as possible.Several studies have shown a correlation between low albumin levels and increased anastomotic leakage.The Colon Leakage Score (CLS) is a standard score for predicting anastomotic leakage, but this score does not include the albumin level as a parameter of anastomotic leakage despite the importance of the albumin level’s contribution on the anastomotic leakage. Therefore, this study sought to analyze the correlation between the albumin level, CLS, and the incidence of anastomotic leakage after anastomotic resection surgery in colorectal cancer (CRC) patients at Dr. Hasan Sadikin General Hospital Bandung, Indonesia. This study was a cross-sectional retrospective analytical study on medical records of CRC patients over the age of 18 who underwent intestinal anastomotic resection surgery between 2016–2020. There were thirty-two patients with colorectal cancer underwent anastomotic resection during the study period, with two patients experienced anastomotic leakage. Correlation analysis showed that the correlation coefficient for the albumin level and anastomotic leakage was 0.209, while the correlation coefficient between CLS and anastomotic leakage was 0.110. There is no correlation between albumin level, CLS, and the incidence of anastomotic leakage after anastomotic resection surgery in CRC patients treated at Dr. Hasan Sadikin General Hospital Bandung, Indonesi
Laparoscopic Ventral Mesh Fixation in Patients with Pelvic Organ Prolapse
Get PDFPelvic organ prolapse (POP) is a condition with a high incidence rate and often creates difficulties in surgical coloproctology and gynecology. The aim of this study was to evaluate the effectiveness of laparoscopic ventral reticular sacrocolporectopexy and sacrorectopexi in women and men with POP, respectively. This study was conducted at the Educational-Surgical Clinic of Azerbaijan Medical University and Department of Surgery of the Faculty of Medicine of Ankara University (2016-2019) on 21 patients with POP (15 women and 6 men). Results of diagnostics and surgical treatment of POP were studied with preferences towards endoscopic, radiation, and functional methods. The surgical methods used in these patients included laparoscopic fixation methods (sacrocolpopexy, sacrocolporectopexy) of protruding organs (uterus, vaginal vault/vaginal cuff, rectum) and simultaneous vaginal (colporrhaphy, colpolevatoroplasty, vaginal plastic surgery) and proctological surgeries (circulatory resection, hemorrhoidectomy, sphincteroplasty). The findings demonstrated that the most progressive POP mostly occurred in women of premenopausal age and during menopause. Based on the results of the long-term evaluation of the surgical treatment (6-12 months), the rates of recurrence of prolapse and complications were low (up to 4.8% and 9.5%, respectively) with favorable long-term functional results, such as a decrease in the degree of fecal incontinence and constipation, observed in the evaluation. Due to the concomitant weakness of the ligamentous apparatus of the pelvic floor in these patients, there is the need for intra-abdominal apical support of organs. In conclusion, that laparoscopic sacrocolpopexy in women and sacrorectopexy in men are reliable surgical method to treat POP. However, specific skills need to be acquired by both gynecologist and coloproctologist to be able to do these laparoscopic surgery techniques
Effect of High Fat Diet on Histopathological Appearance of Pregnant Wistar Rat’s Liver
Get PDFIndonesia is facing increased consumption of calorie-dense foods, such as fast food, among its society. Changes in diet and physical activities towards unhealthy ones can result in an imbalanced lifestyle, leading to various consequences, including hyperlipidemia. Pregnant women experience significant increases in plasma concentrations of lipids, lipoproteins, and apolipoproteins. Excessive cholesterol levels in pregnant women will result in the formation of fat in the liver. The purpose of this study was to determine the effect of a high-fat diet to pregnant Wistar rats (Rattus norvegicus) on the histopathological features of the liver. A true experimental with post test only control group design was applied in this study, which was conducted from August 2020 to November 2021. Fourteen (14) pregnant female rats were divided into two groups. The first group of rats was fed with a high-fat diet, while the second group, which was the control group, was given a standard diet. The high-fat diet used was 3 mL/rat/day beef brain. After the rats delivered their litters, the rats’ livers were retrieved, then examined histopathologically under a microscope. This research was conducted from No fatty liver was identified in the control group or the treatment group. Hence, a high-fat diet does not affect the liver histopathological features in pregnant Wistar rats
DMBA-induced Modulate Estrogen Receptors α and β Breast Cancer’s Animal Model
No full textThe high incidence of breast cancer cases in the world requires the use of applicative methods. The 7,12-dimethylbenz(a)anthracene (DMBA) induced breast cancer animal model is a widely used chemical-induced animal models for research on breast cancer. However, the molecular mechanism related to DMBA induction remains unclear. Good understanding on DMBA-induced animal models is crucial for studies related to future breast cancer treatments as animal models will provide a deeper understanding of anticancer medication, specifically those aimed for treating breast cancer. The aim of this study was to develop an DMBA-induced animal model for breast cancer. This study used female Wistar rats injected subcutaneously with DMBA as a carcinogen-induced agent (20 mg/kg) to induce tumor. Rat tumors were then evaluated and breast appearance was observed weekly, starting from day 28th after DMBA injection. Breast cancer tissue was then sampled and stored at -80°C until it was used for western blot and histological study. This study indicated that DMBA induced cancer in female Wistar rat’s breasts, and cytoplastic cells and lung metastatic was identified macroscopically and histopathologically. The metabolic sign was observed in the lung and breast sections. Interestingly, the DMBA induction in this study does not only induce organ cancers but also induces estrogen receptors and stimulates signaling of estrogen receptors α (ERα), ERβ, and Akt
Factors Associated with Mortality in COVID-19 Patients Treated at Serui General Hospital, Papua Indonesia
Get PDFCoronavirus Disease 2019 (COVID-19) has a high fatality rate and has caused around 4,771,408 deaths worldwide as of 30 September 2021. COVID-19 infection presents various severity levels, starting from asymptomatic to severe symptoms that lead to death. Mortality in COVID-19 patients is associated with multiple risk factors. This study aimed to analyze the risk factors associated with COVID-19 mortality in Serui General Hospital, Papua, Indonesia. A cross-sectional analytic observational study was performed in Serui General Hospital from September 2020to September 2021. Subjects were COVID-19 positive inpatients who met the inclusion and exclusion criteria. The independent variables were demographic characteristics, clinical manifestations, comorbidities, and lab findings, while the dependent variable was mortality. Statistical analysis was performed using chi-square test and logistic regression. This study included sixty-nine patients with 21 (30.4%) of them were non-survivors. The multivariate analysis showed that an NLR of >3.13 (OR 7.49; p=0.037; 95% CI 1.63–34.35), hypertension (OR 4.71; p=0.019; 95% CI 1.28–17.29), and age (OR 4.26; p=0.028; 95% CI 1.17–15.52) were associated with mortality in COVID-19 patients. Thus, NLR >3.13, hypertension, and age are considered to be the risk factors associated with the mortality caused by COVID-19 among patients in Serui General Hospital
Association Between Maternal Mid-Upper Arm Circumference and Baby's Birth Weight
Get PDFBirth weight can be a potential predictor for short-term and long-term health outcomes. Low birth weight can be an output from maternal malnutrition. Mothers' chronic energy malnutrition risk is detected by measuring maternal mid-upper arm circumference (MUAC). This study aimed to analyze the correlation of maternal MUAC with a baby's birth weight. This study was an analytic observational study that used secondary data from October 2021 to January 2022. Data were obtained from Tanjungsari Intergenerational Study Nutrition Working Group from 2014 to 2016. Subjects were 124 pairs of mother and baby who were recruited using total sampling. Data were analyzed using t-test and logistic regression. A significant association of maternal MUAC and the baby's birth weight (t (122) = 3.75, 95% CI 174.5, 564.0, p=0.000) was detected. Underweight (OR 23.3, 95% CI 2.6, 209.0, p=0.005), overweight, and obese (OR 0.05, 95% CI 0.01, 0.42, p=0.006) status was shown to have an association with MUAC. Education level and occupation status had no associations with MUAC. In conclusion, maternal MUAC is associated with baby's birth weight. The determinant factors of maternal MUAC were underweight, overweight, and obese status