Medical Laboratory Technology Journal
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Impact of Lead (Pb) Exposure on Hematological Parameters, hs-CRP, Ferritin, and Oxidative Stress (MDA) in Battery Factory Workers
No full textExposure to lead in the workplace is common, with most employers and workers unaware of its adverse health effects. This study examines the relationship between blood lead levels in battery factory workers and health examination results such as Hematology profile (Hemoglobin level, Leukocyte count, Hematocrit Level, Platelet count, Erythrocyte Count, MCV, MCH, and MCHC), malondialdehyde (MDA), ferritin, and hs-CRP. The samples of this study were 25 blood samples of battery factory employees in Jakarta who were older than 40 years and had worked for more than 1 year. Workers' blood samples were collected using vacuum tubes and placed in ice boxes to be sent to PT Petrolab Service Laboratory for blood lead level examination, Citama Hospital Laboratory Unit for blood profile examination, ferritin level, and hs-CRP value, and Prodia Jakarta Clinical Laboratory for MDA level examination. Bivariate analysis examined the relationship between lead levels, blood profile, ferritin levels, MDA levels, and hs-CRP values. Using the entered formula, multivariate analysis was used to test the relationship between lead levels and blood profile, ferritin levels, MDA levels, and hs-CRP values. The significance level used to test the significance of the relationship was <0.05. The bivariate test results showed there was a correlation between blood lead levels and hs-CRP levels (P=0.000), MDA levels (P=0.000), ferritin levels (P=0.000), Hb levels (P=0.000), hematocrit levels (P=0.006), MCV value (P=0.000), and MCH value (P=0.004). In contrast, Multivariate analysis showed lead levels significantly correlated with MDA level (P=0.014), ferritin level (P=0.005), and MCV value (P=0.013). Blood lead levels should be controlled to reduce the risk of oxidative stress and its impact on health, and it is hoped that workers in contact with lead will place more emphasis on occupational safety and health
The Effects of Fermented Buffalo Milk (Dadih) Fortified with Red Dragon Fruit and Selenium on Adiponectin and Tumor Necrosis Factor-Alpha Levels in Obese Rats
No full textThe increasing prevalence of obesity and its associated complications necessitates the exploration of functional foods as therapeutic interventions. This study aimed to analyze the effects of fermented buffalo milk (dadih) fortified with red dragon fruit (Hylocereus polyrhizus) and selenium on adiponectin and Tumor Necrosis Factor-Alpha (TNF-α) levels in obese rats. This experimental study employed a pretest-posttest control group design involving 20 Wistar rats divided into four groups: K1 (healthy control), K2 (obese control), P1, and P2 (obese intervention). K2, P1, and P2 were in obesity conditions induced by a high-fat-fructose diet (HFFD) for 28 days, and K1 was a healthy control group. Furthermore, dadih only and dadih fortified with red dragon fruit and selenium at 1.8 g/200 g body weight/day were administered to P1 and P2 groups, respectively. Treatment using dadih fortified with red dragon fruit and selenium significantly reduced weight gain (p=0.000) compared to K2 and P1. In addition, the P2 group showed increased adiponectin levels and decreased TNF-α levels (p=0.000) compared to the K2 and P1 groups. A strong negative correlation was found between adiponectin levels and TNF-alpha and body weight, while TNF-alpha and body weight showed a strong positive correlation. To conclude, fortifying dadih with red dragon fruit and selenium is more effective in improving the metabolic condition of obese rats compared to non-fortified dadih. This fortification significantly enhances adiponectin levels and reduces TNF-alpha levels. These findings suggest that functional food fortification could be a promising approach for managing obesity-related inflammation and metabolic disorders in humans, highlighting the potential application of dadih in dietary interventions
Analysis of Serum Creatinine Levels, Blood Pressure and Grade of Hypertension of Chronic Disease Management Program Participants
No full textIndonesia is one of the countries with the highest number of cases of hypertension in the world; the predominant patient is the elderly. In some patients with hypertension, increased blood pressure causes impaired salt and creatinine excretion, resulting in poor kidney function. A chronic disease management program is a health care system with a proactive-integrative approach to prevent further complications. The research objective is to analyze serum creatinine levels, blood pressure, and hypertension grade and determine the relationship between serum creatinine and blood pressure in chronic disease management program participants. The study subjects, 73 participants with hypertension, were obtained by purposive sampling. Patient data and hypertension grade were obtained from medical records; an auto chemistry analyzer analyzed serum creatinine. Data were analyzed using the Pearson correlation test using SPSS. Participants with hypertension fall into the elderly age (60-69 years), totalling 31 (42%); pre-elderly age (45–59 years) includes 28 (38%); and high-risk elderly age (>70 years) includes 14 (19%). Most of the participants (40 participants) fell into hypertension stage II, followed by hypertension stage I (25 participants), and only 8 participants fell into the elevated category. The mean serum creatinine of participants with Elevated blood pressure criteria was 0.89±0.178, Hypertension grade I 0.91±0.322, and hypertension grade II 0.99±0.269, illustrating a trend of increasing serum creatinine levels due to higher blood pressure criteria. Pearson correlation test shows a significant correlation between both systolic blood pressure with serum creatinine levels (p<0.05, r=0.2) and diastolic blood pressure with serum creatinine levels (p=0.007, r=0.342). There was a trend of increasing serum creatinine levels corresponding to the hypertension grade in participants. Blood pressure was significantly correlated with serum creatinine, clearly confirming that an increase in blood pressure may followed by an increase in serum creatinine
The Analgesic Effect and Toxicity of Red and White Ginger on Mus Musculus with the Acetate Writhing Test
No full textBased on data from the World Health Organization in 2020, the incidence of dysmenorrhea was 1,769,425 (90%) women who suffered from dysmenorrhea, with 10-16% suffering from severe dysmenorrhea. The problem of pain is very disruptive to activities, so prevention and treatment of pain must be a concern. Ginger is a natural ingredient that grows in South Kalimantan and is empirically used to treat pain. The study aimed to determine the analgesic effects and toxicity of red and white ginger from Banjarmasin and Banjar Regency. The nutrient content profile of the soil where the ginger grew was analyzed, and it was determined by nitrogen content, cation exchange capacity, and soil pH. The content of active compound groups was analyzed using specific reagents to determine the presence of active compounds. Determination of total phenolic levels using a colorimetric method using a UV-Vis spectrophotometer. The instant ginger formula was optimized with organoleptic test result parameters. Acute toxicity tests using male Wistar rats with observations for 14 days. Analgesic tests were conducted on Mus musculus using the acetic acid writhing test. The results showed that the highest nitrogen (1,34%), cation exchange capacity (29.22 me/100 g), and soil pH levels (6,89) were in the soil where the ginger samples from Banjar Regency grew. The chemical compound content in all gingers contained the same phenolics, flavonoids, tannins, saponins, and terpenoids. The levels of marker compounds in succession from Banjarmasin red ginger, Banjarmasin white ginger, Banjar Regency red ginger, and Banjar Regency white ginger were 7.43%, 6.26%, 8.52%, and 7.12%, respectively. The results of formula optimization showed that all gingers had a sweet and spicy taste, a distinctive odor, powder form, and a reddish-brown color in red ginger and pale yellow in white ginger. The toxicity test results showed that at doses of 300 mg/Kg BW and 2000 mg/Kg BW, there was no toxic effect on the administration of ginger extract. Analgesic activity showed that all gingers had an analgesic effect at a dose of 200 mg/Kg BW. The analgesic effects from the strongest to the lowest were red ginger from Banjar Regency, red ginger from Banjarmasin, white ginger from Banjar Regency, and white ginger from Banjarmasin. This study concludes that red ginger from Banjar Regency has the most potent analgesic activity and no toxicity for all ginger
Increased levels of IL-4 in the Spleen of BALB/c Mice after 65.5 kDa Pili Protein Klebsiella pneumoniae Immunization
No full textPneumonia is an infection of the lung parenchyma caused by Klebsiella pneumoniae, resulting in a high mortality rate of millions each year. To reduce these deaths, one potential solution is to create a vaccine that utilizes virulence factors of this microorganism, such as pili. During the early phase of infection, these antigens have a crucial role and can stimulate the production of memory b cells. These cells are activated by IL-4 (interleukin-4) in lymphoid organs, such as the spleen. Pathogen exposure such as virulence factors can stimulate the secretion of IL-4 in the spleen organ. This study aims to investigate the role of Klebsiella pneumoniae pili in this process. The research conducted from May to December 2023 at the Microbiology Laboratory, Faculty of Medicine, University of Jember was purely experimental, using Balb/c mice with IL-4 levels in the spleen organ as variables. The study used 15 mice, divided into control (K1), adjuvant (K2), and antigen (K3) groups. The research data were analyzed using non-parametric tests, specifically the Kruskal-Wallis and Post Hoc tests. The Kruskal-Wallis test revealed significant differences (p=0.003). In the Post Hoc test, a significant difference was found between the control and antigen groups (p=0.002). The results conclusively demonstrate that induction of Klebsiella pneumoniae pili protein 65.5 kDa significantly increases IL-4 levels in the spleen. Future studies should consider adding serum specimens to provide additional information
The Potential of Probiotics from West Sumatra, Indonesia on Low-Density Lipoprotein and Interleukin-6 Levels with Dyslipidemia
No full textDyslipidemia is a lipid metabolism disorder characterized by abnormal elevations or reductions in one or more lipid fractions within the plasma. This condition is a critical component of both metabolic syndrome and cardiovascular disease (CVD). This study aims to see the potential of curd administration on Low-Density Lipoprotein (LDL) and Interleukin-6 (IL-6) levels in dyslipidemia patients. This study is an experimental study with a pre-post test control group design consisting of a control group and a treatment group totaling 42 people taken randomly at the DR Drs M Hatta Bukittinggi Brain Hospital in June and July 2024. In the three groups, LDL and IL-6 examinations were carried out and measured before and after curd for 14 days. Treatment group 1 was given curd as much as 150 grams, treatment group 2 was given curd as much as 200 grams, given 1x a day, and the control group was not given curd. Data analysis was performed using the Wilcoxon statistical test and paired t-test. Results: There was no significant difference in LDL (p=0.360) and IL-6 (p=0.932) levels after giving curd for 14 days. However, there was a decrease in LDL and IL-6 levels after giving curd for 14 days. Conclusion: The results showed that giving curd for 14 days in the treatment group 1 (150gram) and treatment 2 (200gram) can reduce LDL and IL-6 levels. Consuming probiotics for 14 days can reduce LDL and IL-6 levels
Extract Ethanol of Tempuyung (Sonchus arvensis) Leaves as Anti-Hyperuricemia: In Vitro Studies
No full textHyperuricemia is a condition where there is an increase in blood uric acid levels above 7 mg/dL in men and 6 mg/dl in women. One of the enzymes that plays a role in hyperuricemia is xanthine oxidase which can inhibit uric acid synthesis. Tempuyung leaves (Sonchus avernis) are one of the plants that have antihyperuricemia properties. This study aims to determine the effect of ethanol extract of tempuyung leaves on xanthine oxidase in vitro. Tempuyung leaves come from Palembang, South Sumatra, Indonesia, and are macerated with 96% ethanol until a thick extract is obtained. The xanthine oxidase inhibition test was carried out on tempuyung leaf extract and Allopurinol with respective concentrations of 6.25; 12.5; 25; and 50 ppm and followed by absorption measurements using UV-V spectrophotometry at a wavelength of 293 nm and determining the IC50 value. The research results showed that the ethanol extract of daun tempuyung leaves had an IC50 value of 23.37 ppm, higher than allopurinol of 17.16 ppm which was used as a reference. These results classify the ethanol extract of daun tempuyung leaves as a strong inhibitor of the xanthin oxidase enzyme. Therefore, the ethanol extract of daun tempuyung leaves has the potential to be anti hyperuricemic
Analyze of Parameter Values of Sasirangan Home Industry Wastewater at Hot and Cold Dyeing
No full textThe sasirangan fabric home industry produces wastewater through the hot and cold dyeing process. It is necessary to know the coloring process and wastewater parameter values to design good wastewater treatment. The purpose of the study was to compare the wastewater parameter values including pH, NH3-N, BOD5, COD, TSS, Oil/Fat, Color, Phenol, Temperature, H2S and Crom Total from Sasirangan Home Industry Wastewater at Hot and Cold Dyeing. This type of research is comparative research with static group comparison design. The sample is a portion of wastewater from home industries from in the province of South Kalimantan Indonesia, namely Banjarmasin City, Banjarbaru City, and Banjar Regency. The sampling technique used accidental sampling with a total of 20 samples consisting of 10 samples of the hot dyeing process and 10 samples of the cold dyeing process. Parameters that do not meet the standards in the hot dyeing process are pH, BOD5, COD, TSS, color, and phenol, while in the cold dyeing process are BOD5, COD, TSS, and color. Statistically, there is a significant difference in the parameter values of pH, TSS, color, phenol, and temperature in sasirangan cloth home industry wastewater between the two processes. The results of the research can be used for planning wastewater treatment for the Sasirangan home industry
Rosmarinus officinalis and Centella asiatica Affect Interleukin-6 Zebrafish Larvae Induced High Glucose
No full textGestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose
Formulation Test of Dracontomelon dao (Blanco) Merr. & Rofe Leaf Extract Powder as an Anti-Diarrheal Escherichia coli Method In Vivo
No full textDracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants used in the community. This study aims to analyze the effectiveness of Dracontomelon dao (Blanco) Merr. & Rofe extract powder as an antidiarrheal against Escherichia coli in vivo. Dracontomelon dao (Blanco) Merr. & Rofe leaf comes from Hulu Sungai Utara, South Kalimantan, Indonesia, and is macerated with 96% ethanol—Dracontomelon dao extract powder (Blanco) Merr. & Rofe is made with three parts thick extract, four parts Lactose, and four parts Starch. Experimental rat were conditioned to develop diarrhea and were treated with extract powder with varying doses per kg of body weight, namely 250 mg, 500 mg, and 750 mg. After treatment on day 6, rat recovered from diarrhea in the 750 mg dose treatment group as much as 78%, while the positive control total recovery on day 6 reached 100%. After treatment on day 6, the number of Escherichia coli colonies in rat feces decreased, namely the lowest in the 750mg dose treatment group, 276 colonies/gr. This research concludes that Dracontomelo dao (Blanco) Merr. & Rofe leaf extract powder is effective as an anti-diarrhea against Escherichia coli in experimental rat. Further research is needed on human test subjects to obtain anti-diarrhea drugs with safer and more effective formulations