260 research outputs found

    Respon Imun Spesifik Larva Ikan Mas (Cyprinus carpio) melalui Imunitas Maternal yang Diberi Vaksin Inaktif Whole Cell Aeromonas salmonicida

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    Aeromonas salmonicida are specific bacteria that can cause infections and death to the cultivation of carp (Cyprinus carpio) during larval stage. Death in carp can be prevented by a vaccine, but the vaccine can only be given on the seed over the age of 3 weeks. Maternal vaccination needs to be done to improve the immune system of the larvae by means of inactivated whole cell vaccine A. salmonicida on broodstock ready to spawn. Aims to determine the effectiveness of vaccines on breeders carp to the parent antibody titer test and larvae, as well Survival Rate (SR) and the Relative Percent Survival (RPS) larvae. This research was conducted with a completely randomized design, 4 treatments A (control); B (0.3 ml/kg); C (0.4 ml/kg); D (0.5 ml/kg) and 3 repetitions. The results show that the antibody titer of 0.3 ml dose capable of providing agglutination reaction to pitting 7th (64x dilution) in broodstock, and vaccine doses 0,4ml on broodstock able to give agglutination reaction to the larvae until all 6 wells (32x dilution). A dose of 0.4 ml/kg resulted the highest SR and RPS with 96.11% and 81.25% respectively. Clinical symptoms of redness in control larvae was spread throughout the body whereas on the vaccine treatment was only in certain body parts.Keywords: A. salmonicida, vaccines, maternal immunity, larva, specific immune responÂ

    Pola Rekrutmen, Mortalitas, dan Laju Eksploitasi Ikan Lemuru (Amblygaster sirm, Walbaum 1792) di Perairan Selat Sunda

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    Sunda Strait has high potential of pelagic fish resources, one of which is spotted sardinella (Amblygaster sirm). High market demand of spotted sardinella leads to its high exploitation. Management of sustainable used of this species need various biological information of the target species.The aims of this research wereto analyze of therecruitment pattern, mortality, and exploitation rate of spotted sardinella. Sampling was conducted in April to August 2015 from the catchment landed at Labuan Coastal Fishing Port, Banten. Fish samples taken during the study consisted of  527 males and 245 females. The results showed thatpeak recruitment season for spotted sardinella occurs in Maret (11.64%) and July (13.67%).The natural mortality (M), fishing mortality (F), total mortality (Z) for male were 0,33; 5,30; 5,63 year-1 respectively, and for female were 0,51; 7,75;8,27 year-1 respectively. Exploitation rate (E) for male was 0,94 year-1 and for female was 0,93 year-1.Keywords : exploitation, growth, mortality, spotted sardinella, Sunda Strai

    Pengaruh NAA dan BAP terhadap Kultur Jaringan Nenas Tangkit (Ananas comosus (L.) Merr. cv. Tangkit)

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    This study was aimed at investigating the effect of NAA and BAP on the development of basal slip slices in tissue culture of pineapple cv. Tangkit. The experiment was conducted at the Plant Biotechnology Laboratory Agricultural Faculty University of Jambi from July through to October 2016. Six level of NAA concentrations (0, 1, 2, 3 , 4 and 5 mgL-1) were tested in combination with six levels of BAP concentration (0, 1, 2, 3 , 4 and 5 mgL-1). A Completely Randomized Design with 5 replicates was employed in this trial. Data on the percentage of explants forming shoots, time required for shoot formation, number of shoots growing per explants, and callus proliferation were recorded. Results showed that without the involvement of plant growth regulators there had been no growth on cultured explants. However, shoot growth was found on explants cultured on medium supplemented with 1 – 3 mgL-1 NAA or 1 – 5 mgL-1 BAP. The application of NAA higher than 3 mgL-1 was found to suppress shoot growth even in combination with BAP. Shoot formation was found to be fast on medium supplemented with 1 mgL-1 NAA + 4 mgL-1 BAP, but took longer time on medium with or 1 mgL-1 NAA + 5 mgL-1 BAP as well as medium with 1 – 2 mgL-1 NAA only. The same phenomenon was also found on medium with 1 – 2 mgL-1 NAA only. This study also revealed that most of cultured explants regenerated only one shoot, but explants cultured on medium with 1 mgL-1 NAA along with 1 mgL-1 BAP produced 2.33 shoots on the average. The addition of 1 mgL-1 NAA without BAP and 1 mgL-1 BAP without NAA could increase average shoot growth by 1.78 and 1.44, respectively. Keywords:   in vitro culture, micropropagation, naphthalene acetic acid, benzyl amino purine.     &nbsp

    Pengaruh Kepadatan Populasi Sitophilus oryzae (L.) terhadap Pertumbuhan Populasi dan Kerusakan Beras

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    The research aims to study the effect population density of S. oryzae against population growth and damage of rice. The population density of S. oryzae is 5, 10, 15, and 20 pairs of adult per 250 g rice. The results showed that population density S. oryzae affects pest population growth of S. oryzae, characteristics loses weight of rice, percentage powder of rice, and changes content moisture of rice. The population density S. oryzae as many as 20 pairs of adult per 250 g of rice can increase populations pest S. oryzae, characteristics loses weight of rice, percentage powder of rice, and content moisture of rice. The relationship between population density S. oryzae with a growing population, percentage loses weight, percentage rice hollow, percentage powder of rice, and changes content moisture of rice increased linearly. There is a positive correlation between population density S. oryzae with population growth S. oryzae (r = 0.997**; P < 0.01), the percentage loses weight (r = 0.987*; P < 0.05), the percentage of rice perforated (r = 0.998**; P < 0.01), the percentage powder of rice (r = 0.997**; P < 0.01), and changes content moisture of rice (r = 0.964**; P < 0.01)Keywords: Sitophilus oryzae, population density, population growth, damage ric

    Pengaruh Rizobakteria Pemacu Tumbuh dan Toleran Kekeringan serta Kelimpahan dan Akitvitas Mikroba Tanah terhadap Tanaman Jagung (Zea mays L.)

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    Agricultural land in Indonesia was largely dominated by untapped potential land, almost of that potential land is dry land. Maize (Zea mays L.) is one of the staple crops that are known could be cultivated on dry land. Rizosphere has been known to containmicroorganisms that can improve the plant growth. In the previously study, we have screenedin vitro of rhizobacteria (6 isolates of Pseudomonas sp CRB and 7 isolates of Bacillus sp CR) as growth promoter of maize and drought tolerant. A total of 6 rhizobacterial formula were further applied in vivo in greenhouse to promote the growth of maize. In planta test on maize showed that formula CRB 19 and F3 (CR 83 + CRB 10) were the best formula that could enhance plant growth parameters under drought stress conditions. After that abundance and activities of microbe were analyzed by enumeration of total fungus, bacteria and actinomycetes, dehidrogease test, soil respiration test dan C-microbe test and those tests showed that formula CRB 19 and F3 (CR 83 + CRB 10) were the best formula.These formula could be recommended as inoculants of maize planted in dry land agriculture.   Keywords : drought tolerant,formulation, growth promoter, maize, rhizobacteri

    Identifikasi Jenis Mikroorganisme pada Tanaman Kurma di Kawasan Tangerang: Identification types of Microorganisms on Date palms roots in Tangerang Region

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    Dates palms (Phoenix dactylifera L.) are species of plants which can be found commonly in the Middle East. The fruits are occasionally found and consumed during Ramadhan. There are several factors which can affect the growth of the plant, mycorrhiza is one of the example. Mycorrhiza is a form of mutualistic symbiosis between the root of plants and fungus. This symbiosis is proven to be favorable for plants, as it can help the absorption of the nutrient, help the defensive mechanism against pathogens, and support the plant to cope with the water deficiency. Furthermore, the plants may also have an alternate form of mutualistic symbiosis with the bacteria called endophytic bacteria which involves in the transaction of nutrient and mineral. This study requires the isolation of microorganisms that can be found within the root of date palms. The isolation can be done with the usage of Potato Dextrose Agar (PDA) as the media. There were several microorganisms which can be found within the isolated plant’s root. Through identification based on its morphology characteristics, the microorganism that was found probably are Staphylococcus; Bacillus subtillis; Glomeromycota sp., and Glomaceae sp. Keywords: Bacteria, dates, Glomaceae sp., Glomeromycota sp., mycorhizz

    Isolasi Bertahap Bakteri Pendegradasi Limbah Tandan Kosong Kelapa Sawit di PT. Erasakti Wira Forestama Muaro Jambi

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    Abstrak. Penelitian ini bertujuan untuk mendapatkan isolat bakteri yang mampu mengurai limbah tandan kosong kelapa sawit secara bertahap. Skrining tahap pertama pada Nutrient Broth dan Tandan Kosong Kelapa sawit yang dishaker 150 rpm selama 3 hari,dan diperoleh 18 isolat bakteri. Sedangkan pada skrining  tahap kedua  menggunakan Nutrient Broth ditambah Tandan Kosong Kelapa Sawit dan diperkaya dengan sisa medium tahap 1 (Nutrient Broth ditambah Tandan Kosong Kelapa Sawit yang telah didegradasi oleh 18 isolat)  dishaker  selama 3 hari dengan kecepatan 150 rpm, didapatkan 6 buah isolat bakteri. Menurut buku Bergey Manual of Determinative Bacteriology Isolat 1, isolat 2, isolat 3, isolat 5 dan isolat 6 termasuk kelompok ke 7 yaitu bakteri gram negatif aerobik bentuk kokus dan batang. Sedangkan isolat 4 termasuk ke dalam  kelompok ke 14 yaitu bakteri gram positif berbentuk kokus.   Kata Kunci : Isolasi, Limbah, Tandan Kosong Sawi

    Analisis Vegetasi dalam Komunitas Nepenthes mirabilis di Hutan Kampus Institut Agama Islam Negeri Sultan Thaha Saifuddin Jambi

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    Abstrak. Nepenthes merupakan tumbuhan unik yaitu mempunyai kemampuan untuk mencerna serangga yang terperangkap dalam organ berbentuk kantong pada ujung daun untuk memenuhi kebutuhan proteinnya. Nephentes dapat dijadikan sebagai tanaman hias yang memiliki nilai komersial tinggi dan juga sebagai obat-obatan. Sumatera merupakan wilayah terbesar kedua dari penyebaran Nepenthes setelah Kalimantan. Habitat alami dari jenis Nepenthes di Sumatera setiap tahunnya semakin terancam, baik oleh pembalakan liar, kebakaran hutan maupun konversi lahan hutan. Penelitian ini bertujuan untuk menganalisis jenis vegetasi dalam komunitas Nepenthes mirabilis di hutan kampus Institut Agama Islam Negeri Sulthan Thaha Saifuddin Jambi. Penelitian ini merupakan penelitian kualitatif. Metode yang digunakan adalah metode transek. Berdasarkan hasil pengamatan tumbuhan yang ditemukan di sekitar komunitas Nepenthes mirabilis pada plot pengamatan adalah sebanyak 17 famili, yang terdiri dari 26 jenis dan 3547 individu. Jenis tumbuhan yang paling banyak ditemukan di sekitar komunitas Nepenthes mirabilis adalah dari famili Poaceae yaitu Axonopus compressus sebanyak 1336 individu, pada famili Gleicheniaceae yaitu Dicranopteris linearis sebanyak 470 individu dan pada famili Leguminoceae yaitu Mimosa pudica sebanyak 419 individu. Sedangkan jenis tumbuhan yang paling sedikit ditemukan pada famili Polygalaceae dengan jenis Polygala paniculata terdapat 6 individu.   Kata Kunci: Vegetasi, Komunitas, Nepenthes mirabilis

    IDENTIFIKASI SPEECH RECOGNITION MANUSIA DENGAN MENGGUNAKAN AVERAGE ENERGY DAN SILENT RATIO SEBAGAI FEATURE EXTRACTION SUARA PADA KOMPUTER

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    Abstrak Biometric recognition merupakan sistem pengenalan  atau identifikasi seseorang  berdasarkan karakteristik  biologis khusus yang dimiliki oleh orang tersebut, fungsinya  untuk sistem keamanan  dengan  mengenali identitas seseorang. Speech recognition merupakan suatu  proses yang dilakukan  komputer untuk mengenali kata yang diucapkan oleh  seseorang tanpa mempedulikan identitas orang terkait. Pola peningkatan dan penurunan signal suara merupakan ciri khas (feature extraction) dari seseorang dalam hal berbicara , sehingga antara energy rata-rata (Average Energy) bunyi  dan lamanya ambang diam  (Silent Ratio) merupakan faktor yang terpenting untuk mendeteksi karakter dan pola suara seseorang. Sedangkan proses algoritma Fourier Frequency Transform (FFT) digunakan untuk mengcirikan komponen-komponen data pada domain frekuensi. Hasil pengujian yaitu menunjukkan bahwa suara seseorang yang direkam dengan durasi dua detik kemudian dilakukan pembagian sinyal gelombang selama 0,2 detik diperoleh besarnya Average energy adalah 2.323 dan proses Silent ratio sebesar 0.249. Kedua nilai tersebut merupakan feature extraction suara seseorang yang dapat dikenali oleh komputer.   Kata kunci: Avarege Energy, Fourier Frequency Transform, Speech recognition, Silent Rati

    Jumlah Sulur sebagai Penanda Diabetes Mellitus Tipe-2 Etnis Minangkabau

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    Abstrak. DMT-2 merupakan penyakit degeneratif yang sulit disembuhkan. Prevalensi diabetes di Indonesia menduduki peringkat ketujuh di dunia. Oleh karena itu upaya yang diperlukan untuk mencegah DMT-2 dengan mencari penanda genetik. Jumlah sulur ujung jari tangan dapat digunakan sebagai penanda genetik DMT2. Tujuan penelitian menemukan hubungan jumlah sulur  sebagai penanda genetik DMT-2. Metode penelitian case control dengan model cross sectional study dengan 66 DMT-2 dan 66 kontrol. Hasil penelitian jumlah sulur ujung  jari tangan DMT2 adalah 104,85 dengan SD ±30,02, pada kontrol 122,06 dengan SD ±29,54. Hasil uji-t dengan nilai p<0,05. Jumlah  sulur ujung  jari tangan kanan   DMT2 adalah 53,3 dengan SD ±15,4, sedangkan pada kontrol  63,2  dengan SD ±15,9. Jumlah  sulur ujung  jari tangan kiri DMT2 adalah 51,6 dengan SD ±15,9, pada kontrol 58,9  dengan SD ±15,5. Hasil uji anava didapatkan p<0,05. Hasil uji anava jumlah sulur setiap jari diperoleh p<0,05, kecuali jari V/kelingking. Dengan demikian terdapat hubungan jumlah sulur total sidik jari(TRC), jumlah sulur tangan kanan dan kiri serta   jumlah sulur setiap sidik jari dengan penderita DMT2 etnis Minangkabau, kecuali kelingking.   Kata kunci: jumlah sulur, DMT2 dan etnis Minangkaba

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