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PENGARUH KEDALAMAN TERHADAP PERKEMBANGAN RUMPUT LAUT KOTONI (Kappaphycus alvarezii) HASIL KULTUR JARINGAN
Full text linkThis research aims to determine the best depth of the development of seaweed seeds produced by tissue culture. The trial was carried out for VI weeks using the same initial weight of seeds which was 75 grams on 3 levels of different water depths: 10 cm, 30 and 50 cm. Parameters observed included plant development, and survival rate. The results of observations of plant development at a depth of 10 cm from the first week to the week to VI the initial weight of 75 g, 115.80 g, 157.40 g, 216.00 g, 303.00 g, 341.00 g and 375.00 g. 30 cm depth initial weight 75 gr, 118,60 gr, 139,80 gr, 199,00 gr, 254 gr 293 gr and 304 gr. 50 cm depth: initial weight 75 gr, 96,6 gr, 122,4 gr, 159 gr, 207 gr, 250 gr and 260,8 gr. Survival rate (SR) at a depth of 10 cm from the first week to the fourth week was 100% and at the fifth and sixth weeks 98% respectively. SR at a depth of 30 cm from the first week to the fourth 100% and the fifth and sixth weeks 96%. And SR at a depth of 50 cm from the first week to the fourth week 100% and at the fifth week 94% and 92%. Based on the results of calculations, the development of plants at a depth of 10 to 30 cm, is relatively the same and gives better results than at a depth of 50 cm. This means that in conducting production activities can be planted at a depth of between 10 to 30 cm.Penelitian ini bertujuan untuk mengetahui kedalaman terbaik terhadap perkembangan bibit rumput laut hasil kultur jaringan. Uji coba dilakukan selama VI minggu dengan menggunakan berat awal bibit yang sama yaitu 75 gram pada 3 tingkatan kedalaman perairan berbeda : 10 cm, 30 dan 50 cm. Parameter yang diamati meliputi, perkembangan tanaman, dan sintasan. Hasil pengamatan perkembangan tanaman pada kedalaman 10 cm dari minggu pertama sampai minggu ke VI berat awal 75 gr, 115,80 gr, 157,40 gr, 216,00 gr,303,00 gr, 341,00 gr dan 375,00 gr. Kedalaman 30 cm berat awal 75 gr, 118,60 gr,139,80 gr, 199,00 gr, 254 gr 293 gr dan 304 gr. Kadalaman 50 cm : berat awal 75 gr, 96,6 gr, 122,4 gr, 159 gr,207 gr, 250 gr dan 260,8 gr. Sintasan (SR) pada kedalaman 10 cm dari minggu pertama hingga minggu keempat 100 % dan pada minggu ke lima dan keenam masing-masing 98 %. SR pada kedalaman 30 cm dari minggu pertama hingga keempat 100 % dan minggu kelima dan keenam 96 %. Dan SR pada kedalaman 50 cm dari minggu pertama hingga minggu ke empat 100 % dan pada minggu ke lima 94 % dan 92 %. Berdasarkan hasil perhitungan, perkembangan tanaman pada kadalaman 10 sampai 30 cm, relatif sama dan memberikan hasil lebih baik dibanding pada kedalaman 50 cm. Hal ini berarti dalam melakukan kegiatan produksi dapat melakukan penanaman pada kedalaman antara 10 hingga 30 cm.  
Keragaman Morfometrik dan Gen cytochrome b DNA Mitokondria Ompok hypopthalmus di DAS Batang Hari.
Full text linkThis research aims to analyse the variation on morphometric and cytochrome b (cyt b) DNA mitocondria gene of Ompok hypopthalmusin Batang Hari river. Cyt b DNA mitocondria gene was amplified by polymerase chain reactionand targeted1104bp nucleotids. The results show there is no significant differentiation on morphometric structure of the samples. This result is consistent for both of morphometric (p=0.96) and index (p=0.99). Amplification of cyt b DNA mitocondria gene generates927 bp nucleotids with cytosin as the majour component and guanin as the lowest. Only one nucleotide(0.11%) is vary among all the sequences i.e.883thof the partial cyt b gene. The 883th nucleotidein O. hypopthalmus Simpang and Sungai Bengkal is guanin, while inMandiangin dan Pelayangan is adenin. This subtitution occured on the first codon and changed the 295th putative amino acid ofcyt b gene. The putative amino acid in O. hypopthalmus Simpang-Sungai Bengkal is alanina (GCC), while inMandiangin-Pelayangan is treonina (ACC).Tujuan penelitian ini adalah untuk menganalisis keragaman morfometrik dan gen cytochrome b (cyt b) DNA mitokondria Ompok hypopthalmus di DAS Batang Hari. Data struktur morfometrik diukur untuk 12 karakter. Amplifikasi gen cyt b DNA mitokondria dilakukan secara polymerase chain reaction (PCR) menggunakan primer cbkr 1: 5’-CCCGAAAAACTCACCCCTTA-3’ dan cbkr 2: 5’-ATAGCCCGGTTAGAGGGTTT-3’,dan hasil amplifikasi dirunut dengan target 1104bp nukleotida. Hasil analisis menunjukkan bahwa struktur morfometrik O. hypopthalmus di DAS Batang Hari tidak berbeda nyata antar lokasi pengambilan sampel. Hasil ini konsisten baik untuk karakter morfometrik (p=0.96) maupun indeks (nisbah) struktur morfometrik (p=0.99). Analisis gen cyt b DNA mitokondria menghasilkan runutan sepanjang 927 bp dengan komposisi terbesar berupa sitosin,dan komposisi terkecil adalah guanin. Hanya terdapat satu nukleotida (0.11%) yang berbeda pada seluruh runutan O. hypopthalmus DAS Batang Hari yaitu nukleotida ke-976 dari gen cyt b utuh. Nukleotida pada O. hypopthalmus dari Simpang dan Sungai Bengkal adalah guanin, sedangkan pada O. hypopthalmus dari Mandiangin dan Pelayangan adalah adenin. Substitusi nukleotida ini terletak pada kodon pertama dan mengakibatkan terjadinya perbedaan asam amino putative ke-309. Asam amino ke-309 pada O. hypopthalmus Simpang-Sungai Bengkal adalah alanina(GCC), sedangkan pada O. hypopthalmus Mandiangin-Pelayangan adalah treonina(ACC)
Molecular Characteristics of Giant Gourami from Ciamis and Banyumas
Full text linkGiant gourami has been widely cultivated across Java Island, including in Banyumas and Ciamis Regencies. However, information genetic characters of both populations were not available. Therefore, this study aims to evaluate molecular characteristics of giant gourami from Ciamis and Banyumas. A purposive random sampling was done Molecular characterization was performed using randomly amplified polymorphic DNA. Molecular data were analyzed statisticaly. Both populations were genetically different and they have high genetic diversity within population. Based on the high genetic diversity of giant gourami from Ciamis and Banyumas populations, it would be very good to crossbreed between those both to produce high quality offspring.  
Jenis-jenis udang air tawar dan karakteristik habitat di tujuh sungai Kabupaten Aceh Barat Provinsi Aceh
Full text linkThis study aims to determine the species of freshwater shrimp and habitat character in District West Aceh. The research conducted in April - July 2014, include at seven observation site, Used purposive sampling methode. At each station, samples were collected for 30-45 minutes with a length of 3-5 m sampling area along the river in a direction against the current. Samples were taken to the Basic Biology Laboratory of STKIP Bina Bangsa Meulaboh, for further identification. The identification of shrimp based on morphological features, taxonomic characteristics and body measurements. Shrimp habitats in each site are described and documented. There are five species of freshwater shrimp found in West Aceh, ie: Macrobrachium rosenbergii, M.empulipke, M. equidens, M. australe, and M. lanchesteri. There are different species of Freshwater shrimp found in different habitat characters
Respon Fisiologis Kedelai [Glycine max (L.) Merr.]Varietas Grobogan terhadap Tingkat Naungan yang Berbeda
Full text linkThe study aimed to analyze physiological responses of soybean varieties Grobogan to different shade levels, including changes in amount of leaf total protein content, photosynthetic pigments content, index stomata and stomata gap width and vegetative growth. The research was conducted using a Completely Randomized Design (CRD) one factor, that is shading level with three treatments: no shading, 50% shade and 70% shade. Data were analyzed by ANOVA and continued by DMRT at 95% confidence level. The results showed that Soybean varieties Grobogan is able to adapt and survive under 50% and 70% shade. Varieties Grobogan responded to increased shade by increasing leaf total protein content and photosynthetic pigments content and decreasing index stomata and stomatal gap width. Shaded plants showing stunted vegetative growth, seen by decreased of leaf number, leaf area, leaf thickness, stem height, stem diameter, root length and plant weight.Penelitian bertujuan menganalisis respon fisiologis kedelai varietas Grobogan terhadap tingkat naungan berbeda, meliputi perubahan kadar protein total daun, kadar pigmen fotosintetik, indeks stomata dan lebar celah stomata serta pertumbuhan vegetatif tanaman. Penelitian menggunakan Rancangan Acak Lengkap (RAL) satu faktor berupa perbedaan tingkat naungan yaitu: tanpa naungan, naungan 50% dan naungan 70%. Data dianalisis menggunakan ANOVA dan dilanjutkan dengan uji DMRT pada taraf kepercayaan 95%.Hasil penelitian menunjukkan bahwa kedelai varietas Grobogan mampu beradaptasi dan bertahan hidup di bawah naungan 50% dan 70%. Kedelai varietas Grobogan merespon peningkatan taraf naungan dengan meningkatkan kadar protein total daun dan pigmen fotosintetik serta menurunkan indeks stomata dan lebar celah stomata. Tanaman ternaungi mengalami pertumbuhan vegetatif yang terhambat dilihat dari penurunan jumlah daun, luas daun, tebal daun, tinggi batang, diameter batang, panjang akar dan bobot tanaman
Analisis Perbedaan Jumlah Bakteri Pada Ikan Nila (Oreochromis niloticus) Budidaya
Full text linkFish have good prospect to be developed because have high economic value. However, the percentage of bacterial fish diseases reached 33.9%. Tilapia (O. niloticus) is one of the most common bacteria. The TPC results of tilapia ponds of I, II, and III cultivation are 1.32 X 105 Cfu/g; 1.7 X 105 Cfu/g; 1.14 X 105 Cfu/g. From the results of physics measurement parameters of three ponds cultivation is still in accordance with the classification of water quality, but if viewed from the results of chemical measurement parameters pond culture II pertained water polluted.Ikan mempunyai prospek cukup baik untuk dikembangkan karena memiliki nilai ekonomi yang cukup tinggi. Namun, persentase penyakit ikan akibat bakteri mencapai 33,9%. Ikan nila (O. niloticus) termasuk yang sering terserang bakteri. Hasil TPC dari ikan nila kolam budidaya I, II, dan III secara berturut-turut adalah sebesar 1,32 X 105 Cfu/g; 1,7 X 105 Cfu/g; 1,14 X 105 Cfu/g. Dari hasil parameter pengukuran fisika ketiga kolam budidaya masih sesuai dengan klasifikasi mutu air, namun jika ditinjau dari hasil parameter pengukuran kimia kolam budidaya II tergolong air tercemar
Efektivitas Pemberian Bacillus sp. D2.2 pada Media Teknis Molase terhadap Kualitas Air dan Performa Pertumbuhan Udang Vaname (Litopenaeus vannamei): The Effectivity of Bacillus sp. D2.2 in Molasses Technical Medium to Water Quality and Growth Performance of Vaname Prawns (Litopenaeus vannamei)
Full text linkVanamei prawns have fast growth and can reared in high density. it has an impact on water quality deterioration and disruption of survival rates and growth. Various ways to cope has been done, one of them is with probiotic bacteria. The new strain of D2.2 bacteria is thought to be effective of ammonia utilization. Probiotics with local bacteria Bacillus sp. D2.2 is cultured on molasses technical medium to be applied semi-mass. The purpose of this study is to asses the efectivity of Bacillus sp. D2.2 inthe molasses technical medium on water quality and growth performance of vaname prawns (Litopenaeus vannamei). The research was used complete randomized design (RAL) with four treatments, A (Control), B (Application of 5 ppm Bacillus sp. D2.2 cultured in molasses technical medium), C (Application of 10 ppm Bacillus sp. D2.2 cultured in molasses technical medium), D (Application of 15 ppm Bacillus sp. D2.2 cultured in molasses technical medium) were repeated three times each. The results showed no effect on water quality and shrimp survival rate, but absolute growth (W), daily growth rate (GR) and feed conversion ratio (FCR) showed that B and C treatment had better than control.
Keywords: Vaname shrimp, growth, Bacillus sp. D2.2, molasses technical mediu
Keanekaragaman Jenis Kupu-kupu di Taman Nasional Karimunjawa Jawa Tengah: Diversity of butterflies in Karimunjawa National Park of Central Java
Full text linkKarimunjawa National Park has several types of ecosystems that hold high biodiversity potential and have not been fully recorded. Karimunjawa National Park is the habitat of flora and fauna including butterflies there. The objective of the research was the diversity of butterfly species in Karimunjawa Island. The study was conducted on August-September 2017. The method of the research using Point Count method. Data analysis using diversity index, evenness index, dominance index, and similarity index. The results showed that the index of diversity (H') of butterfly community on Karimunjawa Island has value (2.95), it indicates that the condition of the butterfly community is stable. The result of the Eveness Index obtained a high yield E = 0.76, it shows that the individual distribution of each type of butterfly on Karimunjawa Island is evenly distributed. The result of the dominant Index shows a low value (0.10), thus no dominance is concentrated on a particular type of butterfly. Analysis of similarity index of species in all habitats observed obtained results IS= 53-73%, this shows that in the three habitats observed can be inhabited by almost all butterfly species and Has an equally important role as a community of butterflies in Karimunjawa Island.
Keywords: biodiversity, butterfly, Karimunjawa Islan
Adaptasi Ikan Timpakul (Perioptholmodon schlosseri) di Habitat terganggu Muara Sungai Barito, Kalimantan Selatan: Adaptation of Timpakul fish (Perioptholmodon schlosseri) in disturbed habitat of Barito river estuary, South Kalimantan
Full text linkTimpakul fish (Periophthalmodon schlosseri) is a kind of fish which able to live long in the land. One of the habitats of the fish is Barito river estuary. The development of land conversion which high enough at Barito river estuary into the area of fishponds and rice fields cause P. schlosseri must adapt to the environment disturbed. The research aimed to determine the adaptation of P. schlosseri in fishponds and rice fields habitats in Barito river estuary. The research is conducted by using the method of line transect, that is by walking in the area of rice fields and fishponds in Barito river estuary with the object of observation consisted of nest and activity of P. schlosseri. The results of observation showed that the density of P. schlosseri was relatively small at 0.54 individual/hectare in paddy fields areal and 0.84 individual/hectare the area of aquaculture. The relatively low density of fish was caused by nature solitary and carnivorous fish. The adaptation of P. schlosseri in the areas of fishponds and paddy fields could be successful because it was supported by adequate food source, that were crab, shrimp, frog and small fish and predators were relatively few.
Keywords: Adaptation, Barito river estuary, disturbed habitat, Periophthalmodon schlosser
Analisis Isi Lambung Ikan Selais Terang Bulan (Kryptopterus bicirrhis, Valenciennes 1840) di Desa Rantau Kasih Sungai Kampar Kiri Propinsi Riau: Study on Gut content of Selais Terang Bulan fish (Kryptopterus bicirrhis, Valenciennes 1840) in Rantau Kasih village Kampar Kiri river, Riau Province
Full text linkThe study on gut content of Selais Terang Bulan fish (Kryptopterus bicirrhis) in Rantau Kasih village Kampar Kiri river was conducted from Februari-April 2017. The purpose of this study was to know about gut content of Selais Terang Bulan fish which was categorized as main food, supplementary food and additional food. The analysis is done based on the instruction of Natardjan and Jhingran (1961). The gut content was analized using the Index of preponderance. The result revealed that the main food of Selais Terang Bulan fish in Rantau Kasih village is adult Arthropoda with IP value (78.85%), and supplementary food is caterpillar (25.15%). Male and female fishes at have main food of adult Arthropoda with different percentages are (82.81%) male and female (71.32%). Based on the gut content analysis of Selais Terang Bulan fish was a carnivorous fish.
Keyword: gut content analysis, Kryptopterus bicirrhis, Kampar kiri rive