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ヨードホルム・水酸化カルシウムパスタ(糊剤根管充塡材ビタペックス)の組織埋入に関する実験的研究 第1報 病理組織学的検索
Get PDFapplication/pdfUsing the rat the tissue reaction to calcium hydroxide added idoform (Vitapex^[○!R]:Root cannal filling material) was studied by means of radiography, histopathology and enzyme histochemistry. A small amount of the paste was implanted into the subcutaneous tissues, muscles and the periosteums. Experimental period belongs between 4 days and 61 days (Table 1). Results are as follows: 1. Radiographic examination revealed that the implanted paste showed a well demarcated radiopaque mass just after the experiment, then gradually diffused and was absorbed, and that the paste was disappeared approximately 40 days later (Figs.1-15). 2. Histopathologically, granulation tissues gradually formed around the Vitapex. The round cells consisted chiefly of histiocytes (Figs.16, 17). 3. These histiocytes were demonstrated to contain calcium salts by the von Kossa stain (Figs.18,19). 4. A lot of foam cells were observed around the Vitapex, showing histiocytes phagocytized the silicone oil in the paste (Figs.20-22). 5. Foregin body giant cells appeared adjacent to the Vitapex (Fig.22),which contained many von Kossa possitive granules in the cytoplasm (Fig.23). 6. In some cases, implanted the paste into subcutaneous tissues and muscles, irregular calcified tissues had formed near the paste, but they could not be identified to be distinct bone tissues (Fig.24). 7. In some specimens, implanted the paste near the bone, some irregular bone had formed at the surface of the original bone(Fig.25). 8. Only one case demonstrated a small but clear induced bone adjacent but free to original bone (Fig.26). It contained some osteocytes (Figs.26,27 arrows). 9. Although both alkaline and acid phosphatase activities were observed in the granulation tissues in the early period (Figs.28,29), the former had to disappear (Fig.30) and the latter remained (Fig.31) in the 2 months later. 10. Based upon above mentioned facts the Vitapex was considered to be excellent for root canal filling.journal articl
小児患者に対する局所麻酔の臨床的研究第1報 下歯槽神経ブロック
Get PDFapplication/pdfAs a more reliable and safe technique of the inferior alveolar nerve block for young children, a new presumption method of the position of the mandibular foramen was made to attempt clinically. Felling the top of the condyle, the Gonion, the coronoid notch, and the posterior border of the ramus, the goal of the needle point was estimated at "a half of the height, a half of the width of the mandibular ramus". Under this technique of the block, one hundreds and thirty one children, aged from two years to twelve years, were treated, and anesthetic effect, duration and complications were clinically evaluated. The results are as follows: 1. Almost always, satisfactory anesthetic effects were obtained. Only two cases required an additional injection, and in all cases the scheduled dental procedures were completed. 2. The average number of treated teeth was 2.4 in one appointment. Varies dental procedure were completed under the block, such as restorations, pulpotomies, pulpectomies, extractions and combinations of these. 3. In spite of the short action anesthetics, 3% Citanest with 1 : 300,000 epinephrine, the duration of the soft tissue's anesthesia was estimated at 123 ± 40min.(mean±s.d.) 4. No complications or aversive reations were observed during the treatments. After the treatments, three young children chewed their lips or tongues, no other remarkable complications were observed.journal articl
Periapical Cemental Dysplasiaの1症例
Get PDFapplication/pdfThe lesion appeared at the root apex of the lower right second premolar of a 64-year-old woman. Roentgenographically, it was characterized by a well-defined radiopaque mass. Histopathological appearances showed that the cementum consisted of regular layers and irregular layers both containing a number of entrapped cells. However, there was no Mosaic-like structure in the cementum. So, the lesion was diagnosed as periapical cemental dysplasia, in mature inactive stage.journal articl
ヨードホルム・水酸化カルシウムパスタ(糊剤根管充填材ビタペックス)の組織埋入に関する実験的研究 第2報 電子顕微鏡的検索
Get PDFapplication/pdfUsing 21 rats the reactions of subcutaneous tissues to calcium hydroxide added idoform (Vitapex^[○!R]: Root canal filling material) were studied by means of electron-micro-scopy. Resalts were as follows: 1. Collagen fibers were gradually formed around the paste. Needle-like calcium crystals were appeared on the collagen fibers. 2. In the area of sparsely distributed collagen fibers and matrix vesicles contained with electron-dense materials, the growth of crystals beyond the calcified matrix vesicles was obserbed. 3. It was suggested that the matrix vesicles came from the regressive changes of cells. 4. The calcium salts, phagocytized within histiocytes, were demonstrated as electron-dense masses. 5. Silicone oil in the paste was also phagocytized by histiocytes, showing the droplets in the cytoplasm. 6. It was considered that histiocytes had the phagocytotic share, because there were no histiocytes containing both calcium hydroxide and silicone oil. 7. Many lysosomes were observed along the phagocytosis. The lysosomes fused with the phagosomes, and then the cytoplasm of histiocytes was occupied by many phagolysosomes.journal articl
イオン導入電気歯刷子応用による象牙質知覚過敏症への効果
Get PDFapplication/pdfThe study was conducted by the double-blind method to determine the clinical effects of an iontophoretic toothbrush used with a sodium fluoride dentifrice of 29 patients of cervical hypersensitivity during 6 weeks experimental period. The following results were obtained. 1) The clinical effect (reduction hypersensitivity) of iontophoretic toothbrush was significantly different from that of placebo group after 2 weeks (P<0.01) and it was highly significant difference (P<0.001) after 6 weeks. In the active group the reduction in sensitivity, after 6 weeks was shown to be 60.6% and in the placebo group 25.4%. 2) Fifty point one per cent of the initially sensitive teeth had lost all sensitivity after 6 weeks. 3) No side effect of this iontophoretic toothbrush was observed, during the experimental period. On the basis of the above facts, it is concluded that an iontophoretic toothbrush used with a fluoride dentifrice is highly effective in the cervical hypersensitivity. Accordingly, the use of an iontophoretic toothbrush is simple and available method for home dental care.journal articl
精密鋳造に関する研究(その9)ワックスパターンに対する埋没材の硬化膨張の影響について
Get PDFapplication/pdfIn this report, the deformations of full crown type wax pattern invested with cristobalite investment were described. They were measured by means of wire strain gauges, which fixed to various positions of the pattern. They were invested in casting rings, sized 40 mm in diameter and length, lined with a sheet of dry Kaowool (a sheet of alumina-silica fibers) or wet asbestos ribbon. Results were follows; 1) The deformations caused by setting expansion of the investment come from not only the expansions but also the shrinkage pressing stresses, which were affected by the capacity of the cushion material. 2) The deformations were unsymmetrical, when the wax pattern was invested aside from the center of the ring. In this condition, a part of the wax pattern, even when it was situated on the center of the ring, was influenced by the deformation. 3) The dimensional change of the wax pattern was 4 microns in diameter, when the investment was mixed with standared water-powder ratio.journal articl
走査電子顕微鏡によるマウス顎下腺の細胞内構造の観察
Get PDFapplication/pdfA variety of procedures have been employed to prepare soft tissues and cells to observe their intracellar stractures by the scanning electron microscope. However, their procedures often unsatisfactory because of artifact caused by shearing forces and other reasons. The present study attempt to examine successful procedure for intracellar stractures of mouse submandibular glands. After cryofracture was used, mild ion-etching (700V) or low-pH, hypotonic solution (without ion-etching) method was applicated for disclose intracellar stractures of their cells. Ion-etching specimens were examined by three diffent fixations and effect of ionetching time. Specimens fixed with paraformaldehyde and glutaraldehyde resulted in better ionetching effect than those fixed with osmium tetroxide. Successuful result for disclose intracellar stractures was obtained from paraformaldehyde and glutaraldehyde fixation, and ion-etching specimens for 10-15 minutes. Without ion-etching method did not get good results in submandibular glands in present study.journal articl
Marmur変法による株化腫瘍細胞DNAの抽出
Get PDFapplication/pdfThe present study was aimed, firstly to apply the modified Marmur's method (by Hill) in extracting DNA from the cultured tumor cells, and secondly to investigate some properties of the extracted DNA. The cultured tumor cells had been taken from the rhabdomyosarcoma in rats induced by injecting DMBA, as reported before, and been kept in the Department of Oral Surgery II, Matsumoto Dental College. Five samples were taken from the 70th to 80th generation of the cultured cells. The DNA was extracted by employing the modified Marmur's method of Hill, which is excellent in obtaining the pure DNA. The extracted DNA was examined by the ultra-violet spectrograph, using the Hitachi Spectrophotometer Model 101, and weight measurements in the Burton's method. The ratio of the ultra-violet absorption at the 260nm wave length to that at 280nm varied little, with an average of 1.9, which coincided with the studies reported by other investigators. The mean weight measure was 100μg/ml, with slight deviation. These results might indicate that the modified Marmur's method of Hill is excellent for obtaining the pure DNA from the cultured tumor cells of rats.journal articl
