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Inhibition of Mouse Neuromuscular Transmission by Stannic Chloride
Get PDFapplication/pdfThe present study was conducted to determine whether stannic chloride (SnCl_4) at concentrations above 50цM inhibits or facilitates neuromuscular transmission. SnCl_4 decreased the amplitude of the endplate potential (e.p.p.) concentration-dependently in a concentration range from 0.05 to 0.5mM. Not only SnCl_4 (0.5mM) but also tartaric acid (TA, 0.5mM), a solvent for 0.5 mM SnCl_4, decreased the e.p.p.. The action of SnCl_4 was stronger than that of TA. Both SnCl_4 (0.5mM) and TA (0.5mM) decreased the quantal content of the e.p.p., and the action of the former was significantly stronger than that of the latter. SnCl_4 (0.5mM) raised the frequency of the miniature endplate potential (m.e.p.p.), whereas TA (0.5mM) reduced it. SnCl_4 (0.5mM) decreased the m.e.p.p. amplitude as did TA (0.5mM). The results obtained show that SnCl_4 inhibits neuromuscular transmission by decreasing the volume of the transmitters released by nerve impulses.journal articl
Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis
Get PDFapplication/pdfBy anion-exchange chromatography of the concentrated culture fluids of P. gingivalis W 50, two proteolytic enzymes, referred to as RGP-I and RGP-II were, eluted from column at the different NaCl concentrations in the eluant buffer. The two enzymes were purified until the preparations displayed single bands with sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE) by gel filtration and isoelectric focusing. Comparative studies between the two enzymes revealed that the same molecular masses (44 kDa) of RGP-I and II were obtained both with gel filtration and SDS-PAGE. However, the isoelectric points of RGP-I and II were 5.0 and 5.7, respectively. Both enzymes were thiol proteinases and were inhibited by sulfhydryl group blocking reagents, tosyl-L-lysine chloromethylketone, EDTA, leupeptin, L-trans-epoxy-succinylleucylamido-(4-guanidino) butane, and antipain. Both enzymes were activated by glycylglycine. RGP-I and RGP-II hydrolyzed synthetic substrates containing arginine in the P-1 positions, but those containing lysine in the same position were notcleaved.journal articl
Studies on the Castability of Pure Titanium (Part 3) Influence of casting pressure and sprue diameter on the titanium castability
Get PDFapplication/pdfWe analyzed the external defects of castings with mesh grid patterns with 3 different kinds of phosphate bonded casting molds with 2 parameters (sprue diameter and casting pressure). Castability with pure titanium was affected by the parameters of sprue diameter, and casting pressure with different casting molds. The sprue condition was the most affective casting condition in the all directional pressure type casting machine. In 2 types of casting molds, one was strongly affected by the casting pressure in castability and the other was scarcely affected by the casting pressure. The former type of casting mold had a low permeability.journal articl
