Jurnal Ilmu dan Teknologi Peternakan Tropis
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    Pengaruh Penambahan Minyak Ikan Tongkol dan Mikroorganisme Lokal Isi Rumen terhadap Proporsi Telur Ayam Ras

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    ABSTRAK Tujuan penelitian ini adalah untuk mengetahui peningkatan kualitas fisik telur berupa proporsi kuning telur, proporsi putih telur, dan proporsi kerabang dengan penambahan minyak ikan tongkol dari limbah pengalengan ikan dan mikroorganisme lokal (MOL) berbahan dasar isi rumen sapi. Penelitian ini dilakukan di Teaching Farm ayam petelur Politeknik Negeri Jember selama 1 bulan dengan menggunakan ayam ras petelur strain Hyline sebanyak 72 ekor dengan rata rata bobot badan 1,9 kg. Metode yang digunakan adalah rancangan acak lengkap pola faktorial dengan dengan faktor A berupa penambahan minyak ikan tongkol sebanyak 0%; 0,3% dan 0,6% dan faktor B penamahan MOL 0%; 0,3%; dan 0,6%. Setiap faktor diulang 4 ulangan dan setiap unit ulangan terdapat 2 ekor ayam. Penambahan minyak ikan dan MOL dicampurkan pada complete feed pabrikan. data yang diperloeh dianalisis ragam pola faktorial, apabila terdapat perbedaan nyata (p0,05) terhadap proprosi putih telur.Kata Kunci: ayam ras petelur, kualitas fisik telur, minyak ikan tongkol, mikroorganisme lokal The Effect of Adding Cob Fish Oil Waste and Local Microorganism (MOL) from Rumen Contents for Layer Eggs ProportionABSTRACTThe purpose of this study was to determine the increase in physical quality of eggs in the form of egg yolk proportions, egg white proportions, and shell proportions with the addition cob fish oil from fish canning waste and local microorganisms (MOL) based on beef rumen contents. This research was conducted at the Teaching Farm Layer Chickens of the Politeknik Negeri Jember for 1 month using 72 laying hens of the Hyline strain with an average body weight of 1.9 kg. The method used was a completely randomized design with a factorial pattern with factor A in the form of adding 0% cob fish oil; 0.3% and 0.6% and factor B added 0% MOL; 0.3%; and 0.6%. Each factor was repeated 4 replications and each replication unit contained 2 chickens. The addition of fish oil and MOL was mixed in the manufacturer's complete feed. The data obtained were analyzed by various factorial patterns, if there was a significant difference (p0.05) on the proportion of egg whites.Keywords: layer chicken, physical quality of eggs, cob fish oil, local microorganism

    EVALUATION OF PHYTOCHEMICAL COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY ON RED FRUIT PEEL AS FEED ADDITIVES

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    ABSTRAKRed fruit (Pandanus conoideus Lamk) is a native plant from Papua that contains many bioactive compounds such as tocopherol, alpha tocopherol, carotenoids, and flavonoids as well as phenolic, which are potential as antioxidant and antibacterial agents. Red fruit also contains unsaturated fatty acid especially oleic acid (omega-9) which is useful for improving nutritional status of the community along with its mineral contents of Fe, Ca and Zn. The results of this study showed that red fruit peel contains phenolic and flavonoid compounds with test results of 652,49 mg GAE/100g and 143,08 mg/100g respectively. Red fruit peel  has antioxidant activity with IC50 value of 75,785 µg/gr. Antibacterial activity of red fruit peel has a weak inhibition zone but significantly have a good inhibition to Escherichia coli, Salmonella typhi and Lactobacillus plantarum bacteria (P <0,01).Key words: Red fruit peel, phenolic, flavonoid, antioxidant activity, antibacterial activity

    The Physiological Response of Madura Cattle to Dry Matter Consumption Levels on Maintenance at Different Altitude Locations

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    This study aims to know the physiological response of Madura cattle to the level of DM consumption with the same maintenance pattern and the same physiological conditions at different altitudes, namely in lowland areas (Tlanakan District, Pamekasan Regency) and medium plains (Tembalang District, Semarang City). Tlanakan sub-district has an altitude of 0-22 m above sea level, and Tembalang district is 160-250 m above sea level. The results of the study showed that the level of DM consumption at different altitudes gave significantly different results (

    Meat and Bone Ratio of Broiler Fed with Different Proportions of Fish Meal

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    This study aims to determine the ratio of meat and bones of broiler fed a diet containing fish meal with different proportions. The design used in this study was a completely randomized design (CRD) consisting of 4 treatments and 4 replicates and each replication consisted of 6 chickens. The treatment used was commercial feed BP-11 as control feed (P0), 43% corn + 21% rice bran + 27% CAB Concentrate + 9% fish meal (P1), 43% corn + 23% rice bran + 22% CAB Concentrate + 12% fish meal (P2), dan  43% corn + 25% rice bran + 17% CAB Concentrate + 15% fish meal (P3). The variables measured in this study were the percentage of meat weight, the percentage of bone weight, and the ratio of meat and bones of broiler. The data obtained were analyzed by variance analysis and the treatment that affected the measured variables was further tested using Duncan's Multiple Range Test. The results showed that the average percentage of broiler meat in this study was 67.26% (P0), 68.71% (P1), 69.36% (P2) and 70.23% (P3). The averages of bone percentages were 23.18% (P0), 25.53% (P1), 22.48% (P2) and 22.58% (P3). Meanwhile, the ratio of meat and bones were 2.92% (P0), 2.79% (P1), 3.09% (P2), 3.20% (P3). The results of the analysis of variance showed that the treatment had no effect on the percentage of meat, the percentage of bone and the meat-bone ratio. It was concluded that the addition of fish meal up to 15% into diet had no significant effect on percentages of meat and bone, and the ratio of meat to bone.Keywords: broiler, fish meal, meat, bone, rati

    The Potency Of Glutamate Producing Lactic Acid Bacteria Isolated as Additive Feed In Improving Broiler Performance

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    ABSTRACT Glutamate is one of the amino acids that can act as flavor neurotransmitter. Some lactic acid bacteria (LAB) are known as producers of glutamate. Such LAB could be widely found in many fermentation products. The objectives of this study were to isolate and characterize some glutamate producing LAB isolated from West Sumatera fermented food products (dadih, asam durian, ikan budu and tapai). A total of 1,014 LAB was isolated from the fermented food products. Of these, 40 isolates were considered the best based on their ability to produce inhibition zones >2mm in the presence of 2% CaCO3. Qualitative analysis using thin layer chromatography and quantitative analysis using spectrophotometer revealed that 27 of the 40 LAB isolates produce glutamate. The highest glutamate production was found in LAB of dadih origin (coded as N32). N32 produced a glutamate of 38.25 mg/ml. N32 was found to be gram positive, negative for catalase, oxidase and motility, and can utilize sugars in the form of lactose, glucose and mannitol. The N32 was identified to be Lactobacullus sp. Thus N32 (Lactobacullus sp) from dadih has the potential to produce glutamate acid. Keywords: glutamate, building blocks, lactic acid bacteria, food fermentati

    Leukocyte Profile of Dexamethasone-Induced Quail After Administration of Bombyx Mori Silkworm Pupae Extract

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    Poultry especially Japanese quails are susceptible to the environmental threat that induced glucocorticoid hormone secretion. Glucocorticoids stimulate oxidative stress and a decrease in the immune system. The purpose of this research is to study the potential of silkworm extract (Bombyx mori) to overcome oxidative stress and reduced the immune system in quails that were induced with dexamethasone as glucocorticoid. Thirty-two adult quails were used and were given dexamethasone. Those quails were divided into 4 groups, four groups were given 0 mg, 0.16 mg/kg, 0.32 mg/kg, and 0.64 mg/kg of silkworm extract for each quail. The parameters that were observed are total leucocyte count, differential leucocyte count, and heterophil/lymphocyte ratio. The results are not significant compared to the groups that were not given any supplements. This study shows that the administration of silkworm extract is not effective for recovery purposes.Keywords: antioxidant, quail, Bombyx mori, dexamethason

    Profile and Income of Bali Cattle Farmers under Different Farming Systems in Southeast Sulawesi, Indonesia

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    The farmers in Southeast Sulawesi, Indonesia have started using the intensive farming system for Bali Cattle rearing in recent decades. This study aimed to determine the profile and income of Bali Cattle farmers under intensive, semi-intensive, and extensive farming systems in Southeast Sulawesi, Indonesia. The research was conducted using the Participatory Rural Appraisal (PRA) method. Profile and income data were collected from 105 farmers selected by purposive sampling. The analytical model used is one-way ANOVA with the farming system as a factor. The results showed that the age of intensive farmers was younger (p<0.05) and had a lower experience of raising livestock (p<0.05) than extensive and semi-intensive farmers. In addition, farmers in the intensive farming system have a higher goal of raising livestock as fertilizer producers (p<0.05) than extensive and semi-intensive. However, semi-intensive and extensive farming systems had a higher average number of cows, net body weight production yield, income, and value-added (p<0.05) than the intensive farming system. Based on the results, it can be concluded that semi-intensive and extensive farming systems generate high incomes and can be more economically sustainable than intensive systems.Keywords: participatory rural appraisal, animal production system, farming syste

    Potensi Produksi Nutrien Jerami Padi dan Brangkasan Jagung Sebagai Pakan Alternatif Sapi Perah di Kabupaten Konawe

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    ABSTRAKPenelitian ini bertujuan untuk mengetahui kualitas dan produksi nutrien jerami padi dan brangkasan jagung sebagai pakan alternatif sapi perah di Desa Ambopi, Kecamatan Tongauna Utara, Kabupaten Konawe. Sampel jerami padi dan brangkasan jagung dianalisis di Laboratorium Unit Analisis Pakan Ternak, Fakultas Peternakan, Universitas Halu Oleo. Metode penelitian yang digunakan adalah analisis deskriptif dengan penentuan lokasi di lakukan secara sengaja. Di Desa Ambopi Kecamatan Tongauna Utara terdapat 2 jenis brangkasan tanaman pangan yang berpotensi untuk dijadikan pakan alternatif sapi perah yaitu jerami padi dan brangkasan jagung. Kualitas jerami padi dan barangkasan jagung meliputi BK (bahan kering), Abu, PK (protein kasar), LK (lemak kasar), SK (serat kasar), BETN (bahan ekstrak tanpa nitrogen) dan TDN (total digestible nutrient) cukup baik di Desa tersebut. Desa Ambopi Kecamatan Tongauna Utara mampu memproduksi jerami padi sebesar 187,658 ton/ha/tahun dan brangkasan jagung sebesar 0,701 ton/ha/tahun. Potensi BK, PK, TDN jerami padi yaitu 99,86%, 99,86 dan 99,84% sedangkan brangkasan jagung yaitu 0,14%., 0,14 dan 0,16.  Jumlah sapi perah yang dapat ditampung di Desa Ambopi berdasarkan produksi BK brangkasan tanaman pangan adalah sebanyak 60 ST per tahun. Saat in sapi perah yang dipelihara di Desa Ambopi adalah 7 ST. Hal ini berarti jumlah sapi perah yang masih dapat ditampung berdasarkan potensi produksi BK tersebut adalah 53 ST.Kata Kunci: desa ambopi, jerami padi, brangkasan jagung, pakan, sapi perahPotential Production of Straw Rice Nutrients and Corn Stover as Alternative Feeds for Dairy Cows in Konawe District ABSTRACTThis study aims to determine quality and production of rice straw nutrients and corn stover as alternative feed for dairy cows in Ambopi Village, Northern Tongauna District, Konawe Regency. Sampel of rice straw and corn stover analized at the laboratory of nutrition and animal feed, Faculty of Animal Science, Halu Oleo University. Location is determined by purposive sampling and data were processed by destriptive analysis method. The result showed that there were 2 types of agricultural waste which had the potential to be used as alternative feed for dairy cows in Ambopi Village, namely rice straw and corn stover. The quality of rice straw and corn stover is quite good based on the content of DM (Dry Matter) ash, CP (Crude Protein), EE (Extract Eter), CF (Crude Fiber), NFE and TDN. Ambopi Village, is able to produce 187.658 tons/ha/year rice straw and 0.7012848 tons/ha/year corn stover. The potential DM, CP, TDN of rice straw is 99,86; 99,89 and 99,84% while corn stover is 0,14; 014 and 0,16%. The number of dairy cows that can be accommodated is 60 head / year or 60 animal unit (AU). The current number of dairy cows in Ambopi Vilage is 7 AU. It can be concluded that the additional amount of dairy cows which can be accommodated in Ambopi Village is 53 AU.Keywords: ambopi village, rice straw, corn stover, feed, dairy co

    Evaluasi Keragaman Gen Selenoprotein W1 (SEPW1) dan Kaitannya Terhadap Komposisi Asam Lemak Pada Domba

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    Kandungan asam lemak dipengaruhi oleh sejumlah gen dan di antaranya ialah selenoprotein W1 (SEPW1). Eksperimen ini bermaksud untuk mengetahui polimorfisme gen SEPW1 serta hubungannya terhadap kandungan asam lemak di domba. Sejumlah 102 ekor domba yang tersusun dari 20 domba ekor gemuk (DEG), 30 domba ekor tipis (DET), 9 domba komposit garut (DKG), 10 domba compass agrinak (DCA), 10 domba barbados cross (DBC), dan 23 domba jonggol (DJ) digunakan dalam penelitian ini. Analisis polimorfisme gen SEPW1 dicari dengan memanfaatkan Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP), enzim BanI, serta produk amplifikasi berukuran 478 bp. Analisis Chi-square memberikan hasil bahwa populasi berada dalam keseimbangan Hardy-Weinberg, kecuali sampel domba jonggol. Polimorfisme gen SEPW1 teramati berada di semua sampel rumpun domba. Single nucleotide polymorphism (SNP) g.53617063 G>A gen SEPW1 berasosiasi (P<0,05) dengan asam laurat (C12:0), asam palmitat (C16:0), asam arakidat (C20:0), asam henekosilat (C21:0), asam miristoleinat (C14:1), asam oleat (C18:1n9c), asam linoelat (C18:2n6c) dan asam lemak total. Genotipe GG memiliki kandungan asam lemak tak jenuh yang paling tinggi dibandingkan genotipe lainnya. Gen SEPW1 memiliki kapasitas untuk dikembangkan sebagai penanda asam lemak di domba

    Use of Collagenase Enzyme as a Substitute for Trypsin on In Vitro Growth of Liver, Kidney, and Fibroblasts of Chicken Embryo Cells

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    This study aims to determine the effect of using the collagenase enzyme on the viability of chicken embryos' liver, kidney, and fibroblast cells in vitro. Liver and kidney organs were collected from embryonic chicken eggs at 15 and 19 days. Meanwhile, fibroblast cells were obtained using muscle tissue of 11-day-old chicken embryos. Each organ and tissue was chopped and incubated using 0.1% trypsin for 10 minutes and 0.1% collagenase for 30 minutes. Concentration, viability, and proliferation rate were calculated after incubation and analyzed using a t-test. The results showed that there was a significant increase in the tissue incubated with collagenase on the viability and proliferation rate of liver cells, kidney cells, and chicken embryo fibroblast cells (t0, 05). Cell isolation and purification techniques need to be carried out to study the quality of somatic cell culture of chicken embryos more specifically.Keywords: collagenase, trypsin, chicken embryos, viability, cell culture

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