Jurnal Progres Kimia Sains
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ANALISIS RESIDU DETERGEN ANIONIK SODIUM DODECYL SULFATE (SDS) MENGGUNAKAN SPEKTROFOTOMETER UV-Vis
No full textAnalisis residu detergen anionik Sodium Dodecyl Sulfate (SDS) telah dilakukan. Analisis dilakukan dengan metode metilen biru dan diukur dengan menggunakan spektrofotometer Ultraviolet-Visible (UV-Vis). Residu detergen diekstraksi dengan kloroform dan kadar residu dalam ekstrak yang diperoleh diukur pada panjang gelombang 655 nm. Hasil penelitian menunjukkan bahwa konsentrasi terendah residu detergen ditemukan pada lokasi lepas pantai (Pulau Bokori) sebesar 0,216 ppm, sedangkan konsentrasi tertinggi pada daerah Pelabuhan sebesar 0,759 ppm. Uji parameter yang telah dilakukan menunjukkan metode tersebut memiliki persamaan regresi y = 0,330 x + 0,108 dan nilai koefisien korelasi sebesar 0,997. Hasil penelitian menunjukkan bahwa konsentrasi residu detergen belum melampaui ambang batas baku mutu air laut sebesar 1 ppm, berdasarkan Keputusan Menteri Negara Lingkungan Hidup nomor : Kep-02/MenKLH/I/2004 Kata kunci— metilen biru, detergen, spektrofotometer UV-Vi
ANALISIS GEN MERKURI REDUKTASE (merA) PADA ISOLAT BAKTERI DARI TAMBANG EMAS KABUPATEN BOMBANA SULAWESI TENGGARA
No full textPenelitian mengenai analisis gen merkuri reduktase (merA) pada isolat bakteri dari tambang emas Bombana Sulawesi Tenggara telah dilakukan. Tujuan penelitian ini adalah mengetahui kekerabatan bakteri isolat Bombana berdasarkan urutan nukleotida gen merA. Isolasi DNA kromosom divisualisasi menggunakan elektroforesis untuk mengetahui keberadaan gen merA pada isolat Bombana, yang selanjutnya diukur konsentrasinya dengan menggunakan spektrofotometer UV-Vis. DNA kromosom hasil isolasi diamplifikasi dengan teknik PCR menggunakan primer A1s-n.F: (TCCGCAAGTNGCVACBGTNGG) dan A5- n.R: (ACCATCGTCAGRTARGGRAAVA). Ligasi amplikon gen merA dilakukan pada vektor pJET1.2/blunt, kemudian ditransformasi ke dalam sel inang E.coli. Isolasi DNA plasmid rekombinan dilakukan dengan metode lisis alkali dan miniprep kit #K0502. DNA plasmid disekuensing dan dilakukan analisis terhadap gen merA isolat Bombana. Konsentrasi DNA kromosom isolat Bombana 1 adalah 9,2380 µg/mL dengan rasio A260/A280 adalah 1,8. Sedangkan isolat Bombana 2 memiliki konsentrasi DNA sebesar 1,9416 µg/mL dengan rasio A260/A280 adalah 0,3. Hasil sekuensing gen merA, diperoleh urutan nukleotida dengan ukuran206 bp untuk isolat Bombana 1 dan 214 bp untuk isolat Bombana 2. Berdasarkan rekonstruksi filogeni merA menunjukkan jarak (D) kekerabatan pada isolat Bombana 1 terhadap Staphylococcus aureus sebesar 2,2790. Isolat Bombana 2 sebesar 1,9604 terhadap Streptomyces coelicolor A3.Kata kunci: DNA, merA, Sekuensing
BIOENERGI GENERASI II: TEKNOLOGI PEMBUATAN BIODIESEL DARI LIMBAH CAIR PABRIK CRUDE PALM OIL (CPO)
No full textTujuan penelitian ini adalah untuk membuat biodiesel dari limbah cair pabrik CPO, memperoleh data kualitas biodiesel yang dihasilkan, dan mengetahui jenis senyawa metil ester yang terdapat dalam biodiesel. Proses pengolahan biodiesel dari limbah cair pabrik CPO menggunakan dua tahap reaksi, reaksi esterifikasi menggunakan katalis H2SO4 pada suhu 60oC selama 2 jam dan transesterifikasi menggunakan katalis CaO pada suhu 60oC selama 2 jam. Rendamen metil ester yang diperoleh dari variasi konsentrasi katalis CaO 1, 2, 3 dan 4% sebesar 15,98%, 18,22%, 24,25% dan 29,40%. Hasil analisis GC-MS menunjukkan bahwa senyawa penyusun metil ester dari limbah cair pabrik CPO terdiri dari metil heksadekanoat dengan komposisi 12,87%, metil 9-oktadekenoat dengan komposisi 19,98%, metil oktadekanoat dengan komposisi 5,71%, dan metil 8,11-oktadekadienoat dengan komposisi 10,22%. Hasil uji kualitas biodiesel menunjukkan bahwa viskositas, densitas, bilangan iod, bilangan penyabunan, angka setana, dan titik kabut telah memenuhi standar SNI 04-7182-2006 biodiesel Kata kunci: Biodiesel, esterifikasi, transesterifikasi, kualitas biodiese
Prekonsentrasi Dan Analisis Senyawa Renik Fenol Pada Sampel Perairan: Optimasi Kinerja Adsorben Pada Ekstraksi Padat-Cair
Full text linkDissolved phenol in aquatic system has potential risk on human health.Accordingly, quantification of phenol compound presents in aquatic samples by improvedmethodis of significantly required in the field of Environmental Chemistry. This study aimsto evaluate the usefulness of Sep Pak C18 cartridge as the adsorbent of phenol compoundprior to analyzed by Gas Chromatography. Study is focused on the sample pH and flowrate optimization to gain the best performance of cartridge. Results indicated thatadjusting the pH of 1-2 and the flow rate of 5 ml/minute of sample was highlyrecommended. Such a method is relatively accurate within the range of concentration 1.2ppb 15 ppm of dissolved phenol, thus it may be considered as an alternative method toanalyze phenol which exists within trace level in the aquatic systems. The method has beensuccessfully applied to analyze phenol in seawater samples from Losari Beach, Makassar
Kinetika dan Karakterisasi Amiloglukosidase Isolat Aaspergillus niger Dari Limbah Ampas Sagu
Full text linkStudy of characterization and kinetic amyloglucosidase from Aspergillusniger isolateusing sago pith waste has been done. The aims of this research wereto know the optimumsubstrate concentration, metalions effect, and value of KM and vmaks of amyoglucosidase. Theresult of researches were obtained the optimum activity of amyoglucosidaseat 2% (w/v) ofstarch concentration. The activator of amyoglucosidase were Zn2+, Ba2+, Fe3+, and Co2+,while the inhibitor were Sn2+, Ca2+, Cu2+, Cd2+, Mn2+, Hg2+, and Ni2+. The ions of Hg2+ andNi2+were founded stopped of the enzyme activity. The variables of kinetic of amyloglucosidaewere obtained KM and vmaks value were. 4,40 % , 0,49 mU, respectively
Detection Limit Of Carbamate Pesticides Biosensors Based Enzyme Acetylcholinesterase And Cholin Oxidase On Platinum Electrode
Full text linkThe presence of carbamate pesticides in environmental and food poses a potentialhazard to human health and there is a growing interest in their rapid and accurate determinationfor food safety and environmental monitoring. The aim of this research is to designelectrochemical biosensor for analyzingcarbamate pesticides residue in food material.Acetylcholinesterase (AChE) and cholin oxidise (ChO) enzyme was immobilized at platinum (Pt)wire plated with membrane materials of SA 5%, 10%, 15% and GA 25%. Result of this researchshow that for cellulose acetate (SA) 5% the detection limit is 10-7,7 M, for SA 10% the detectionlimit is 10-8,7 M, for SA 15% the detection limit is 10-7,6 M. This a results is approximately equal to2,2 ppb). ), which means that this biosensor is very sensitive for determining carbamatespesticides residue where its detection limit is comparable to to the detection limit of conventionalinstrument such as Gas Chromatography (GC) and High Pressure Liquid Chromatography(HPLC), i.e. respectively 1,5 ppb and 2,0 ppb
Alternatif Pengolahan Limbah Organik Rumah Tangga Untuk Produksi Biogas
Full text linkWaste is a source of organic material that can be recycled into biogas. This studysought to produce biogas from the fermentation of organic waste using anaerobic microbesisolated from sewage and cow rumen fluid. The study was conducted in the laboratory-scaleanaerobic reactor with a volume of 750 mL, at room temperature, incubation time of 7, 14, 21,28, 35, 42, 49 days, waste solids concentration 1%, 3%, 5%, 7% and 10 %. CO2 produced wasmeasured by the method of deposition by solution of Ba (OH) 2 is converted into volumeusing the general equation of the ideal gas. The difference between the total gas volumeproduced by CO2 gas is methane gas volume value (CH4). The results showed the highestproduction of waste solids obtained at 7% concentration, incubation time of 42 days. Biogas isproduced 99.86 mL / g litter, the total amount of biogas produced during the 7 weeks of 537mL / gram of waste
Sintesis dan Karakterisasi Adsorben Lempung Terimpregnasi Oleh 3-merkapto-1,2,4-triazol (trzsh)
Full text linkImpregnation of clay is one of method to increase the adsorption capacity. Synthesisand characterization of clay impregnated 3-mercapto-1,2,4-triazole (TRZSH) has beencarried out. Functional groups of clay was characterized by infrared spectrophotometry whileBET test was performed to determine its specific surface area and porosity. Results showedthat clay impregnated TRZSH comprises silanol (Si-OH), siloksan (Si-O-Si), C-N, C=N, andSH. Spesific surface area of clay impregnated TRZSH of 184.04 m2/g. Such it has porosityvolume and radius of porosity of 123.902 x 10-3 cm/g and 134.672 A, respectively
Respon Ganda Elektrode Kawat Emas Terlapis Polipirol Terhadap Kation Dan Anion Dari Surfaktan Nads
Full text linkThe coating of gold wire electrode as working electrode with polypyrrole throughelectropolymerization by ciclyc voltammetry was performanced in the 0.01 M pyrrole solutioncontaining 1,0 ´ 10-4 M HDS dopant and 0.01 M KCl, KBr, and KI supporting electrolytes,respectively. The coated electrode prepared by KCl supporting electrolyte has the respontoward Na+ cations with highest sensitivity of 44.0 mV/decade in the concentration range of5,0 ´ 10-5 – 1,0 ´ 10-3 M and toward DS- anions with highest sensitivity of 26.2 mV/decadein the concentration range of 1,0 ´ 10-15 – 1,0 ´ 10-12 M. Coated electrode prepared by KBrsupporting electrolyte might respon toward Na+ cations with highest sensitivity of 42.4mV/decade in the concentration range of 5,0 ´ 10-4 – 1,0 ´ 10-3 M and toward DS- anionswith highest sensitivity of 35.0 mV/decade. The coated electrode prepared by KI supprotingelectrolyte has a respon toward Na+ cations with highest sensitivity of 49.2 mV/decade in theconcentration range of 5,0 ´ 10-4 – 1,0 ´ 10-3 M and toward DS- anions with highestsensitivity of 16.5 mV/decade in the concentration range of 1,0 ´ 10-15 – 1,0 ´ 10-9 M
Sintesis Kitosan Nanopartikel dengan Metode Sonokimia, Gelasi Ionotropik, dan Kompleks Polielektrolit
Full text linkThe aims of this research are to find out how to prepare chitosan nanoparticles bysonochemical method; ionotropic gelation; and polyelectrolyte complexes and the influenceof variations in methods of perparation of chitosan nanoparticles to chitosan nanoparticlescharacteristics that include particle size dan functional group. Techniques of data collectionwas conducted by means of the observation of characteristics of chitosan nanoparticles. Theresults shows that the variation of the method of making an impact on the characteristics ofchitosan nanoparticles preparation. The particle size of nanochitosan synthesized bysonochemical, ionotropic gelation, and polyelectrolyte complex is 800, 500, 600 nm,respectively. Analysis of functional groups of chitosan nanoparticles by FTIR shows theabsorption characteristic spectrum of the nanochitosan