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IDENTIFICATION OF PLANT UTILIZATION IN THE LIFE CYCLE CEREMONY OF THE KAILI DA'A TRIBE IN UWEMANJE VILLAGE, KINOVARO DISTRICT, SIGI REGENCY
No full textThe purpose of this study was to obtain information about the types of plants used by the Kaili Da’a tribe in life cycle ceremonies that are still preserved for generations in Uwemanje village, Kinovaro district, Sigi district. The research population is all people who live in Uwemanje Village, Kinivaro District, which are spread over 4 hamlets. The research method uses survey research with the help of interview questionnaires. Interviews were conducted on key respondents and general respondents. Key respondents were selected by purposive sampling as much as 10% of the population, namely the people who were considered to be the most knowledgeable about the ins and outs of this ceremony, such as Sando, traditional leaders, village heads and village priests. Meanwhile, general respondents are people who are recommended by key respondents with a record that they are at least 30 years old and are married or have a family. The data and information in this study were processed descriptively. Types of plants/plants used in life cycle ceremonies consist of 21 species, namely: rice (Oryza sativa L.), coconut (Cocos nucifera L.), banana (Musa paradisiaca L.), cocor duck (Kalanchoe pinata L.), areca nut (Areca catechu L.), turmeric (Curcuma domestica L.), nail paci (Lawsonia enermis L.), bogenvil (Boganvillea glabra L.), pandanus (Pandanus amarylifolius L.), jatropha (Jatropha curcas), forest basil ( Ocimum sanctum L.), moringa (Moringa oleifera L.), shallot (Allium cepa L.), Bengal grass (Eleusine indica L.), hibiscus (Hibiscus tiliaceus L.), peacock flower (Caesalpinia pulcherima L.), betel (Piper betle L.), keluwih (Artocarpus camansi L.), jasmine (Jasminum sambac L.), palm sugar (Arenga pinnata), and red sugar cane (Saccharum officinarum L.). The percentage of utilization of plant parts that are most directly utilized is the leaves which amount to 14 with a percentage of 65.66%, flowers amount to 4 to 16.67%, fruit to reach 3 to 12.5%. The least used parts are rhizomes, seeds, tubers, roots and stems with a percentage of 5.17%
OBAT TRADISIONAL PENINGKAT IMUNITAS TUBUH ETNIS KARO, KECAMATAN SINEMBAH TANJUNG MUDA HULU
No full textTumbuhan berkhasiat obat sudah sejak lama dimanfaatkan oleh masyarakat Etnis Karo, Kecamatan Sinembah Tanjung Muda Hulu sebagai alternatif pencegahan dan penyembuhan penyakit. Penggunaan obat tradisional sebagai alternatif pengobatan lebih banyak dibanding pengobatan secara medis. Penelitian ini bertujuan untuk mengetahui pengetahuan, kepercayaan akan khasiat obat, jenis tumbuhan, bagian tumbuhan yang digunakan, jenis ramuan, cara pengolahan, dan nilai index of cultural significance (ICS) bahan baku yang digunakan. Penelitian ini menggunakan metode wawancara semistruktur untuk responden umum, dan wawancara bebas untuk informan kunci. Dari hasil penelitian, responden dengan kategori usia dewasa hingga tua memiliki pengetahuan dan kepercayaan yang lebih tinggi dalam penggunaan obat tradisional. Sebelas jenis ramuan obat tradisional diperoleh baik yang disajikan secara tunggal dan campuran yang berasal dari 36 jenis tumbuhan dari 21 famili. Bagian tumbuhan paling sering digunakan adalah daun, dan nilai ICS tertinggi (178) adalah jahe. Tumbuhan obat diramu dengan direbus, digiling, atau dikeringkan
INOVASI UBI KAYU TRANSGENIK MENGGUNAKAN TEKNOLOGI CRISPR-CAS9 SEBAGAI UPAYA PEMENUHAN KEBUTUHAN PANGAN MASA DEPAN
No full textJumlah penduduk yang kian meningkat menyebabkan kebutuhan pangan terus meningkat. Hal ini menyebabkan angka ketergantungan akan beras juga meningkat. Padahal hingga saat ini, untuk memenuhi kebetuhan bahan pangan beras, pemerintah masih harus melakukan impor. Untuk itu sebagai upaya menurunkan ketergantungan konsumsi beras masyarakat Indonesia, pemerintah menggalakkan program diverfisikasi pangan. Salah-satu komoditas yang difokuskan dalam program ini yaitu ketela pohon atau ubi kayu. Namun, dalam pemenuhan kebutuhan gizi, ketela pohon masih memiliki kandungan protein yang rendah. Untuk itu dilakukan genome editing dengan teknik CRISPR-Cas9. CRISPR-Cas9 ini adalah teknologi yang bekerja untuk pengeditan genom dengan kemampuan menemukan tempat tertentu di DNA, untuk memotong sebagian kecil DNA di tempat itu, dan mengedit DNA tersebut. Penerapan teknik CRISPR-Cas9 terhadap ketela pohon menghasilkan tanaman yang mengandung protein yang lebih tinggi
APLIKASI HORMON BAP, NAA, AIR KELAPA TERHADAP MULTIPLIKASI PISANG CAVENDISH (Musa acuminata L.) SECARA IN VITRO
No full textThis study aims to determine the effect of proper concentration of coconut water on the shoot multiplication of cavendish banana (M. acuminata L.) with the addition of the NAA and BAP hormones. This study used a Completely Randomized Design (CRD) with 8 treatments. Each treatment repeated 3 times. Therefore, there were 24 experimental units. Each unit using 1 explant so there were 24 explants. The treatments consisted of: A1 (0% coconut water + 0.2 ppm NAA), A2 (15% coconut water + 0.2 ppm NAA), A3 (20% coconut water + 0.2 ppm NAA), A4 (coconut water 25% + NAA 0.2 ppm), A5 (0% coconut water + BAP 2 ppm), A6 (15% coconut water + BAP 2 ppm), A7 (20% coconut water + 2 ppm BAP) and A8 (coconut water 25% + BAP 2 ppm). The parameters observed were days to the emergence of shoots, number of shoots and percentage of the number of shoots. The results showed that the addition of the 2 ppm BAP hormone which is in treatments A7 showed the fastest emergence of shoots with an average of 8 DAP and the average number of shoots that appeared was 2,67 and the percentage of the number of shoots was 88,89%
FITOKIMIA, DAN AKTIFITAS ANTIBAKTERI DARI Etlingera sublimata Poulsen (ZINGIBERACEAE), TUMBUHAN ENDEMIK SULAWESI: Phytochemical and Antibacterial Activity of Etlingera sublimata Poulsen (Zingiberaceae), Endemic Plant to Sulawesi
No full textThe research entitled "Phytochemical Screening and antibacterial activity of Etlingera sublimata Poulsen (Zingiberaceae), an Endemic Sulawesi of Plants was conducted from January 2020 to June 2020, at the Plant Biosystematics Laboratory and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. Samples of E. sublimata were collected from the mountane forests of the Lore Lindu National Park (LLNP), arround Sedoa village, Lore Utara District, Poso Regency The objectives of the research were to analyze the secondary metabolite compounds in E. sublimata plants and to determine the antibacterial activity of E. sublimata leaves extract in againts Salmonella thypii bacteria. Plant samples were extracted by maceration methods with 96% ethanol as a solvent. Actibacterial activity was tested by using agar well diffusion methods. The experiment was designed by Completely Randomized Design (CRD) with seven different concentration of E. sublimata extract (15, 25, 50, 75 and 85%). Extract and standard drugs were prepared in double-distilated water using Nutrient Agar tubes. 2% Chloramphenicol as standard drugs was used as a positive control and sterile aquadest was used as a negative control. The inhibition zone of bacteria and yeast growth around the disk was measured after 18 to 24 h incubation at 37°C. The results showed that the stem contains alkaloids, flavonoids, terpenoids, tannins and saponins. The rhizome containts alkaloid, flavonoids, terpenoids, tannins and saponins, the flowers containts alkaloid, terpenoids and tannins, while the leaves containts tannin and saponin compounds. Leaves extract of E.sublimata has inhibition activity on the cell growth a pathogenic Salmonella thypi bacteria that the effective extract concentration was 50% with an average inhibition zone ​​2.67 mm
STUDI TUMBUHAN BERKHASIAT OBAT PADA SUKU DONDO DI KECAMATAN OGODEIDE, KABUPATEN TOLITOLI, PROVINSI SULAWESI : Study of Drug Effective Plants on The Dondo Tribe in Ogodeide District, Tolitoli Regency, Central Sulawesi Province
No full textThe Dondo Tribe is an indigenous tribe in Ogodeide District. The Dondo Tribe still uses traditional medicine to treat various diseases. The high trust of the Dondo people towards the use of traditional medicine makes people prefer to use traditional medicine. This study aimed to determine the types, parts, and methods of use of plants that have been used as traditional medicine by the bolrians of the Dondo tribe, Ogodeide District, Tolitoli Regency, Central Sulawesi Province. This type of research was descriptive qualitative and the sampling technique was purposive sampling, with structured interview techniques on 11 informants by filling out questionnaires with supporting documentation. The results of the study found that as many as 40 species of plants used as traditional medicine by the bolrians of the Dondo tribe in Ogodeide District which are still preserved today. The percentages of the most widely used plant parts were leaves 55%, stems 11%, rhizomes 11%, roots 5%, fruit 5%, herbs 5%, bark 2%, twigs 2%, tubers 2% and flowers 2%. The percentage of how to use plants as traditional medicine is 65% taken, 26% affixed, 3% seen, 3% washing and 3% smeared
ETNOBOTANI KERAJINAN ANYAMAN DI DESA DAULAUS KABUPATEN BELU NUSA TENGGARA TIMUR INDONESIA
No full textDesa Dualaus di Kecamatan Kakuluk Mesak Kabupaten Belu dikenal sebagai Desa industri kerajinan anyaman yang menghasilkan produk beragam dan bermanfaat bagi kehidupan masyarakat. Informasi tentang penggunaan tumbuhan sebagai bahan anyaman masih sangat terbatas. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang digunakan, proses pembuatan kerajinan anyaman dan produk kerajinan anyaman serta kegunaannya yang dihasilkan di Desa Dualaus Kecamatan Kakuluk Mesak Kabupaten Belu. Metode yang digunakan dalam penelitian ini adalah survei eksploratif dan wawancara. Hasil penelitian menunjukan bahwa jenis tumbuhan yang digunakan sebagai bahan baku kerajinan anyaman adalah pandan hutan berduri (Pandanus tectorius Sol.), lontar (Borassus flabellifer Linn.) dan gewang (Corypha utan Lamk.). Bagian tumbuhan yang digunakan adalah daun dan lidi. Produk anyaman yang dihasilkan sebanyak 15 jenis yang dimanfaatkan sebagai peralatan rumah tangga, upacara adat, aksesoris dan fashion serta perlengkapan sekolah
KARAKTERISTIK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIUM DASAR JERAMI DAN TONGKOL JAGUNG
No full textLaju pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus (Jacq) P. Kumm) dengan medium dasar jerami dan tongkol jagung telah dilaksanakan di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam. Tujuan Penelitian; (a) Untuk mengamati pertumbuhan jamur tiram putih (Pleurotus ostreatus) pada formulasi media limbah jerami dan tongkol jagung; (b) Untuk mengetahui perbandingan dosis limbah jerami dan tongkol jagung yang dapat memberikan pertumbuhan terbaik terhadap jamur tiram putih. Penelitian ini didesain berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari 7 perlakuan dan 3 kali ulangan. Susunan perlakuan merupakan perbandingan antara Serbuk jerami jagung : serbuk Tongkol Jagung yakni P1 (Serbuk Jerami 100%, tanpa Serbuk Tongkol Jagung), P2 (80% + 20%), P3 (60% + 40%), P4 (40% + 60%), P5 (20% + 80%), P6 (Serbuk Tongkol Jagung 100%, tanpa Serbuk jerami), P7 (Serbuk Gergaji 70% + Dedak Padi 20% + Tepung Jagung 10%, tanpa Serbuk Jerami dan Tongkol Jagung). Parameter pengamatan; (a) Media dasar pertumbuhan; (b) Karakteristik mikroskopik hifa; (c) Laju pertumbuhan miselium; (d) Karakteristik makroskopik koloni; (e) Masa inkubasi. Hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P4 yaitu 103 mm dan terendah pada perlakuan P1 yaitu 75 mm. Jumlah koloni (CFU) tertinggi pada perlakuan P7 yaitu 1,6x1012CFU/g dan terendah pada perlakuan P4 yaitu 0,2x1012CFU/g. Waktu inkubasi paling cepat pada perlakuan P7 yaitu 28 hari dan terendah pada perlakuan P4 yaitu 30 hari
PERTUMBUHAN TANAMAN BAYAM AKAR MERAH (AMARANTHUS RETROFLEXUS) YANG DIINOKULASI DENGAN BERBAGAI JENIS JAMUR MIKORIZA ARBUSKULA YANG DITUMBUHKAN PADA TANAH TERCEMAR MERKURI
No full textBayam akar merah (Amaranthus retroflexus) mampu tumbuh pada tanah tercemar logam berat tanpa menunjukkan symptom. Jamur mikoriza dapat meningkatkan pertumbuhan tanaman pada tanah tercemar logam berat. Tujuan penelitian ini yaitu mengetahui pengaruh pemberian jamur mikoriza arbuskular dari jenis yang berbeda terhadap pertumbuhan tanaman bayam pada tanah tercemar merkuri. Pada penelitian ini tanaman bayam diinokulasi dengan inokulum tunggal dari jenis Glomus sp., Acaulospora tuberculata, dan inokulum campuran dari jenis Acaulospora, Glomus, Gigaspora, dan Scutelospora dan tanpa pemberian inokulum sebagai kontrol. Tanaman bayam ditumbuhkan pada tanah yang tidak dicemari dan dicemari dengan logam merkuri sebanyak 8 mg HgCl/kg tanah. Hasil penelitian menunjukkan bahwa pertumbuhan tanaman bayam tidak dipengaruhi oleh tanah yang tercemar merkuri. Pemberian inokulum jamur mikoriza arbuskula cenderung menurunkan pertumbuhan tanaman. Tidak ada perbedaan yang nyata pada jenis jamur yang diberikan dalam menurunkan pertumbuhan tanaman bayam
COMPOSITION AND AMOUNT OF MARINE DEBRIS DISTRIBUTED TO MANGROVE ECOSYSTEMS IN KUPANG CITY, EAST NUSA TENGGARA, INDONESIA
No full textThe unique mangrove root system is a gathering place for small and large waste. The density of the population results in varied activities so that the waste produced in various types, quantities and sizes can be distributed to the mangrove area either through rivers or carried by the wind. The purpose of this study was to determine the type and amount of waste in the mangrove ecosystem in Kupang City. The method used is observation. Sampling was carried out by installing a transect perpendicular to the sea along 70 m at the place with the highest abundance of waste and the thickness of the mangrove reached 50-100 meters. There are 6 transects installed at the study site and each transect is divided into three zones consisting of three quadrants with a size of 30 x 10 m2. The distance between quadrants is 20 m with the first quadrant being at the first point where mangroves are found. The results of the study found that the composition of the types of waste scattered in the mangrove ecosystem of Kupang City were 88,91% plastik with a total of 15,628 pieces, cloth (4,21%) with a total of 640 pieces, other waste (3,97%) with a total of 604 pieces, cork/foam (1,18%) with a total of 148 pieces, rubber (0,57%) with a total of 86 pieces, metal (0,29%) with a total of 44 pieces, glass and ceramics (0,15%) with a total of 11 pieces, then paper and cardboard (0,05%) with a total of 7 pieces. Types of plastic waste, fabrics, others, rubber and metal have a greater amount in the first zone, cork / foam is the most in the second zone then paper and cardboard are most found in the first and third zones, while glass and ceramics are the most in zone three