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ANALISIS KADAR TIMBAL (Pb) PADA BERBAGAI TUMBUHAN DI KAWASAN STASIUN PENGISIAN BAHAN BAKAR UMUM (SPBU) KOTA PALU
Exhaust gas produced by motorized vehicles releases lead (Pb) into the environment and can be absorbed into plant tissues. This study aimed to examine the content of lead (Pb) accumulated in leaves. Sampling used purposive sampling as a sampling method at four gas stations in Palu City. Pb levels were determined by atomic absorption spectrophotometry (AAS) at the Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tadulako University. The results showed that the highest to lowest lead absorption in the leaves was Syzygium myrtifolium Walp; 0.75 mg/l from Tawaeli gas station, Bouganvillea spectabilis Willd; 0.67 mg/l from Maluku gas station, Polyalthia longifolia var. pendula; 0.67 mg/l at Yos Sudarso gas stations, and Dracaena reflexa Lam; 0.58 mg/l from Diponegoro gas station. The concentration of lead (Pb) found in each plant from gas stations is still below the maximum threshold set by SNI 01-7387:2009, which is 0.5-3 ppm. The ambient air content around the gas station is still below the air quality standard for measuring the lead (Pb) 2 g/m3 parameter
PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN
Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant
PHYTOPLANKTON DIVERSITY IN THE WATERS OF LAKE SIBILI PALU CITY CENTRAL SULAWESI
The study "Phytoplankton diversity in the waters of Lake Sibili, Palu City, Central Sulawesi" was conducted in September 2021. This study aims to determine phytoplankton diversity in Lake Sibili, Tawaeli District, Palu City, Central Sulawesi. This study uses a method (purposive random sampling). The identification results obtained ten phytoplankton species, namely Bacillaria sp., Schroedeia pointera, Zygnemopsis circumcarinatum, Spirogyra plorifica, Shroedeia anchora, Biddulphia sp., Actinastrum gracillimum, Nitzschia actinastroides, Pleurotaenium nageli, and Schaeroplea annulina. Phytoplankton diversity index from each observation station with a diversity index value (station I) 1,726, (station II) 1,801, (station III) 1,743, (station IV) 1,647 and (station V) 1,728. Based on this, the level of phytoplankton diversity in Lake Sibili, Tawaeli District, Palu City, Central Sulawesi is classified as medium
UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli: Antibacterial Activity Test of Tembelekan (Lantana camara Linn) Leaf Extracts on the growth of Staphylococcus aureus and Escherichia coli
This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacteria
PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI
Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days
ANALISIS VEGETASI HABITAT Etlingera sublimata Poulsen (Zingiberaceae) TUMBUHAN ENDEMIK SULAWESI DI HUTAN PEGUNUNGAN SEKITAR DANAU KALIMPA’A TAMAN NASIONAL LORE LINDU
Telah dilakukan penelitian dengan judul “Analisis Vegetasi Habitat Etlingera sublimata Poulsen (Zingibercaeae) Tumbuhan Endemik Sulawesi, di Hutan Pegunungan Sekitar Danau Kalimpa’a Taman Nasional Lore Lindu†dari bulan Desember 2018 sampai Februari 2019 dengan tujuan untuk mengetahui struktur dan komposisi vegetasi di habitatE.sublimata di lokasi penelitian. Penelitian menggunakan metode petak ganda yang diletakan secara sengaja (Purposive sampling) yang jumlahnya 5 buah plot berukuran 20x20 m untuk pengamatan tumbuhan tingkat pohon, kemudian di dalamnya dibuat plot kecil secara bersarang dengan ukuran 10x10 m untuk pengamatan vegetasi tingkat tiang, 5x5 m dan 2 X 2 untuk pengamatan vegetasi tingkat pancang serta tumbuhan bawah. Hasil Penelitian menunjukan bahwa vegetasi tingkat pohon didominasi oleh tumbuhan Pandanus sarasinorum dengan nilai INP 45,97%. Untuk vegetasi tingkat tiang INP tertinggi adalah Mallotus barbatus Mull Arg. dengan nilai 37,38%. Sedangkan jenis yang dominan pada vegetasi tingkat pancang adalah Acmena accuminitisima dengan nilai INP 47.24 %. Jenis yang dominan pada vegetasi tingkat tumbuhan bawah didominasi oleh tumbuhan Desmodium gengengticum (L.) DC dengan nilai INP 42,37%
POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH
Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium
EKSPLORASI BAKTERI LOKAL PENGHASIL ANTIMIKROBA DI CAGAR ALAM TANJUNG API TELUK TOMINI SULAWESI TENGAH, INDONESIA
ABSTRACT
Tanjung Api Nature Reserve is a headland located in Tomini Bay Ampana District Tojo Una-Una Regency, Central Sulawesi Province. This study aims to obtain local bacteria isolates as antimicrobial producers. The method used in this research is purposive sampling and screening method using "Agar Dua Lapis" (Double Layer Method). Testing antimicrobial potency using the method to make Well Diffusion (well) using three pathogens of microbial tests (S. aureus, E. coli, and C. albicans). In each well hole is given 500 μL of bacterial isolates each and incubated at a temperature of 37 oC for 24 hours and continued for 48 hours. The observed parameter is a clear zone formed around the well. Antimicrobial-producing bacteria screening results. obtained as many as 24 isolates of bacteria. Testing of antimicrobial potency by looking at the resulting inhibitory zone is obtained five isolates namely S2U1, S3U3, S7U3, S9U3, and S10U3 are the highest isolates of the bland zone of 12.25 mm but only against S. aureus, while S9U3 is the best isolate because it is able to inhibit the four test microbes namely S. aureus by 5.50 mm, E. coli 9.25 mm, and C. albicans 5.50 mm.
Keywords: Exploration of bacteria, antimicrobials, and Nature Reserv
ETNOBOTANI TUMBUHAN OBAT MASYARAKAT DESA MANIS MATA KABUPATEN KETAPANG
The local wisdom of the people of Manis Mata Village in utilizing plants as traditional medicine is still widely practiced. Public knowledge about medicinal plants is passed down from generation to generation. The people of Manis Mata Village make a lot of use of traditional medicinal plants, but there is no documented data that it is feared that the natural habitat of the plants will be lost due to forest fires, logging of trees, clearing of oil palm land and other natural disasters. The purpose of this study was to determine the types of plants used as traditional medicine by the people of Manis Mata Village. The research method used is descriptive method with triangulation data collection techniques, namely observation, interviews and documentation. Information about medicinal plants was obtained from 94 informants consisting of traditional healers, and people in Manis Mata Village. The results of the research obtained 46 medicinal plant species consisting of 26 families and the most widely used part of the leaves and plants which were most commonly found as medicine was the families zingiberaceae.
Kearifan lokal masyarakat Desa Manis Mata dalam memanfaatkan Tumbuhan sebagai obat Tradisional masih banyak dilakukan. Pengetahuan masyarakat tentang Tumbuhan obat diwariskan secara turun-temurun. Masyarakat Desa Manis Mata banyak memanfaatkan tumbuhan obat tradisional, tetapi belum ada data yang didokumentasikan dikhawatirkan habitat alami tumbuhan akan hilang akibat kebakaran hutan, penebangan pohon, pembukaan lahan kelapa sawit dan bencana alam lainnya. Tujuan penelitian ini untuk mengetahui jenis-jenis tumbuhan yang dimanfaatkan sebagai obat Tradisional oleh masyarakat di Desa Manis Mata. Metode penelitian yang digunakan metode deskriftif dengan teknik pengumpulan data triangulasi yaitu observasi,wawancara dan dokumentasi. Informasi tentang tumbuhan obat diperoleh dari 94 orang informan yang terdiri dari pengobat Tradisional, dan masyarakat di Desa Manis Mata dari hasil penelitian diperoleh 46 spesies tumbuhan obat yang terdiri 26 famili serta bagian yang paling banyak digunakan daun dan tumbuhan yang paling banyak ditemukan sebagai obat yaitu famili zingiberaceae.
 
STUDI ETNOBOTANI TUMBUHAN OBAT TRADISIONAL OLEH MASYARAKAT DI KELURAHAN SETAPUK KECIL SINGKAWANG
Ethnobotany is the use of medicinal plants by the loval environment. Many of the setapuk kecil environment still use plants as traditional medicines that have been passed down from generation to generation. Environment knowledge about traditional medicine is obtained from grandmothers and parents as well as experiences that are taught directly through oral or of these plants will be lost due to forest fires, the conversion of forest land into fields. This will certainly be lost if it is not preserved. he purpose of this research is to find out what types of plants are used as traditional medicines by the people of Setapuk Kecil Singkawang. Information about the use of herbs as traditional medicine was obtained from the local environment as informants. The number of informants was 89 people consisting of traditional healers, village shamans, medicinists, massage artisans and local people. The technique of collecting information is using triangulation technique. There are 43 species of medicinal plants that belong to 31 families to treat 22 diseases as traditional medicine. The most widely used families were 3 species of zingiberaceae, 3 species of piperaceae, and the most widely used plant parts were leaves of 26 species.
Etnobotani merupakan pemanfaatan tumbuhan oleh masyarakat setempat. Masyarakat setapuk kecil masih banyak yang menggunakan tumbuhan sebagai obat tradisional yang diwariskan secara turun temurun. Pengetahuan masyarakat tentang pengobatan secara tradisional didapatkan dari nenek dan orang tua serta pengalaman-pengalaman yang diajarkan secara langsung melalui lisan ataupun praktik. Data tumbuhan obat tersebut belum didokumentasikan. Habitat alami dari tumbuhan tersebut di khawatirkan akan hilang akibat dari kebakaran hutan, pengalihan lahan hutan menjadi ladang. Hal ini tentu akan dapat hilang apabila tidak dilestarikan. Tujuan dari penelitian ini untuk mengetahui jenis-jenis tumbuhan apa saja yang dimanfaatkan sebagai obat tradisional oleh masyarakat setapuk kecil singkawang. Informasi tentang penggunaan tumbuhan tumbuhan sebagai obat tradisional diperoleh dari masyarakat setempat sebagai informan. Jumlah informan sebanyak 89 orang yang terdiri pengobat tradisional, dukun kampung, peramu obat, tukang urut dan masyarakat setempat. Teknik pengumpulan data secara triangulasi yaitu obsevasi, wawancara dan dokumentasi. Hasil penelitian didapatkan 43 spesies tumbuhan obat yang tergolong ke dalam 31 famili untuk mengobati 22 penyakit. Famili yang paling banyak digunakan yaitu zingiberaceae sebanyak 3 spesies, dan piperaceae 3 spesies, serta bagian tumbuhan yang paling banyak digunakan yaitu daun sebanyak 26 spesies