Journal of Integrated -OMICS
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High-throughput genomic technology in research of virulence and antimicrobial resistance in microorganisms causing nosocomial infections: DOI: 10.5584/jiomics.v4i2.167
No full textMost hospital-acquired infections are caused by organisms common in the general population, in which are relatively harmless. Infection by nosocomial pathogenic bacteria is increasingly becoming a major threat to the patients in the hospital. Molecular diagnosis of antibiotic resistant organisms such as Clostridium difficile Infections (CDI) Methicillin Resistant Staphylococcus aureus (MRSA), Extended-Spectrum β-lactamase (ESBL) Escherichia coli, Vancomycin Resistant Enterococcus (VRE), Carbapenem-Resistant Klebsiella (CRK) among others is vital for prevention of healthcare-acquired infections in acute care facilities. DNA microarray besides being a promising diagnosis tool may also provide valuable information about the mechanisms of antimicrobial resistance and pathogenicity of these bacteria. This review aims to highlight the prominence of high-throughput genomic tools in research of virulence and antimicrobial resistance in microorganisms causing nosocomial infections
Vol. 8, No. 2 (2018): DOI: 10.5584/jiomics.v8i2.254
No full textVOL 8, No 2: SPECIAL ISSUE No 1 (2018)
DOI: 10.5584/jiomics.v8i2.25
THE FORENSIC APPLICATION OF PROTEOMICS FOR THE STUDY OF THE TIME OF DEATH: AN OPERATIVE EXPERIMENTAL MODEL FOR PMI ESTIMATION: DOI: 10.5584/jiomics.v8i3.236
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Post Mortem Interval (PMI) estimation is one of the most important questions the forensic pathologist must answer. To date, it is not possible to establish exactly the hour of death, but only to calculate a period (PMI), during which death most likely occurred. In the forensic field, several laboratory methods can be used to perform this calculation more accurately. However, there is still no biomarker that is universally validated and accepted by the forensic community. In the literature, researches about the application of proteomics for forensic purposes are on the increase. Proteomics is a branch of molecular biology that allows the systematic identification of the proteome from a quantitative and qualitative point of view. Below, we propose the operating model of an experimental study currently underway at the Department of Legal Medicine of the University of Catanzaro. The model is based on taking of peripheral blood samples from patients who died at the Intensive Care Unit (AO “Mater Domini” of Catanzaro). The proposed operating model has several advantages including the evaluation, for the first time, of human biological samples from the exact moment of death. The analysis would allow to identify new potential biomarkers expressed in peripheral blood and validate the forensic application of markers already known in the literature. The knowledge of the exact moment of death (time 0) would allow us to evaluate the proteomic profile more accurately on the human model, overstepping the limits of some extrinsic variables evidenced in the literature
An Automated Magnetic Dispersive Solid-phase Extraction Method for Detection of Cocaine and its Metabolites in Human Urine: DOI: 10.5584/jiomics.v8i3.221
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In this work, an automated magnetic dispersive solid-phase extraction (AMDSPE) sampling method followed by high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed for quantitative enrichment of COCs from human urine, using modified magnetic nanoparticles as absorbants. The methodolgy was validated according to internationally accepted regulated acceptance criteria in order to establish the validity of the combination of the AMDSPE and use of HPLC-MS for quantitative analysis. The proposed device significantly improved the sampling preparation efficiency with 32 samples in one batch within 40 mins. Optimization of the preparation procedure for the magnetic nanoparticles was explored and the performances of magnetic nanoparticles were characterized by scanning electron microscopy, vibrating sample magnetometer and infrared spectra measurements. Several analytical experimental parameters were studied, including amount of particles and elution solvent. The automated procedure exhibited acceptable linearity( r>0.9972 ) over the concentration range of 10 to 200ng/mL, The limits of detection for the cocaine and cocaine metabolites were 0.23-1.5ng·mL-1 with recoveries ranging from 77.1 to 94.7%. Compared to traditional sampling method, this method is time-saving and environmentally friendly
Molecular physiological mechanism on consecutive monoculture problems of Rehmannia glutinosa: DOI: 10.5584/jiomics.v1i2.70
No full textRehmannia glutinosa, a famous Chinese medicinal plant, is not suitable for consecutive monoculture, because there are autotoxic metabolites excreted by its tuberous roots, which can greatly limit the plant growth and development. In this research, cultivar “Wen 85-5” R. glutinosa under three diTerent cultivation modes, including the newly planted, the two-year and three-year consecutively monocultured, was used in the $eld test. +e diTerential expression of leaf proteins, physiological changes and corresponding medicinal quality of tuberous roots at the early tuberous root enlargement stage were detected and compared in diTerent years of consecutive monoculture. +e results showed that consecutive monoculture resulted in decrement of chlorophyll content, photosynthetic capacity and root activity, but increases in free radicals and lipid peroxidation. Furthermore, the content of catalpol, the main medicinal ingredient in R. glutinosa tuberous roots was analyzed by FTIR and HPLC. +e result showed that consecutive monoculture resulted in declined medicinal ingredients. Comparative proteomics analysis revealed 20 diTerentially expressed protein spots in response to increasing years of monoculture. Among them, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) kinase, Rubisco, sedoheptulose-1,7-bisphosphatase related to Calvin cycle, and other proteins, i.e. proteasome, malonyl CoA-ACP transferase, antioxidases, pathogenesis-related protein and mRNA-binding protein were down-regulated with increasing years of monoculture. While energy metabolism related proteins (ATP synthase subunit β, ATPase, ATP-binding protein) and stress response related proteins (heat shock proteins) were up-regulated. +erefore it was concluded that consecutive monoculture of R. glutinosa remarkably aTected the physiological reactions and induced the changes in the expression of leaf proteins, this in turn had a negative impact on the biomass and its quality of the medicinal plant
Special Issue: Book of Abstracts ISPROF 2017: DOI: 10.5584/jiomics.v7i2.212
No full textSPECIAL ISSUE: SELECTED ABSTRACTS OF THE III INTERNATIONAL CAPARICA SYMPOSIUM ON PROFILING (ISPROF 2017)
FLIPBOOK VERSION - http://books.bioscopegroup.org/books/djot