Journal of Integrated -OMICS
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Preface - Shotgun proteomics: where do we stand now? DOI: 10.5584/jiomics.v8i1.251
No full textDOI: 10.5584/jiomics.v8i1.25
Long non-coding RNA in regulation of plasticity of vascular smooth muscle cells: DOI: 10.5584/jiomics.v9i1.265
No full textPhenotypic plasticity of vascular smooth muscle cells (VSMCs) is a functional property that is essential for vascular remodeling in vessel injury healing. During phenotypic switch, quiescent VSMCs loss contractile capacity but acquire ability to proliferate and migrate to the injured site where they differentiate again to the quiescent state. However, in pathological conditions such as endothelial dysfunction or atherosclerosis, phenotypic changes in arterial VSMCs become deregulated leading to elevated VSMC dedifferentiation, proliferation, excessive extracellular matrix deposits, and intimal thickening. VSMC hyperplasia is a complex mechanism that is coordinated by a network of various regulatory factors. Long non-coding (lnc)RNAs represent an important part of this regulatory network. Some of lncRNAs are involved in VSMC differentiation, apoptosis, and maintenance of VSMC quiescence, while other lncRNAs promote VSMC dedifferentiation, proliferation, and motility. In this review, we characterize these RNAs and their function in the context of possible involvement to atherosclerosis
Special Issue: Selected abstracts of the III International Caparica Conference in Antibiotic Resistance (IC2AR 2019): DOI: 10.5584/jiomics.v9i2.312
No full textSpecial Issue: Selected abstracts of the III International Caparica Conference in Antibiotic Resistance (IC2AR 2019
Creation of Antifungal Antibiotics of New Generation by Chemical Modification and Genetic Engineering Methods– Modern Approach to a Solution of the Problem of an Antibiotic Resistance at Candidiasis İnfections: DOI: 10.5584/jiomics.v10i1.288
No full textOne of the key and urgent problems in modern medicine and pharmacology in recent decades has become the problem of ineffective action of antibiotics or antibiotic resistance. Immunity of organisms to the action of antibiotics has led to the search for new effective drugs. Treatment of fungal infections, especially candidiasis, is also one of the most important problems in modern medical mycology. The solution of this problem was the creation of new forms of antifungal drugs that are more effective than their predecessors. Molecular transformation of antimycotics was carried out by the help of chemical modification and genetic engineering. Chemically modified antifungals of new generation were tested. Among them pimaricin, amphotericin B, nystatin and lucenzomycin. The positive effect of these drugs on test-cultures of different types of candidiasis in vitro was shown
Peculiarities of changes in parameters of component body composition of representatives of the youth age period detected by the method of bioimpedance analysis: DOI: 10.5584/jiomics.v10i1.283
No full textThe results of the study of body composition parameters using bioimpedance analysis of representatives of the youth age period of 14–19 years are presented. It is determined that the intracellular mass of biological objects, related to the total volume of liquid in it, increases with age of the object. It has been suggested that the amount of intracellular liquid relative to the total liquid in this age group increases due to an increase in the number of cells (since tissue growth occurs due to active cell division), as well as due to a decrease in the number of extracellular liquid, most often caused by an increase in adipose tissue. This pattern is observed for female and male contingent of the studied. The distributions of relative intracellular liquid depend significantly on the age and vary from approximately uniform to normal. The specific basal metabolism for males between the ages of 14 and 19 is increasing, which is explained by the increasing secretion in this category of the studied hormones of the anterior pituitary – somatotropic hormone or growth hormone. It is in puberty, especially in males causes a pronounced acceleration of linear growth and, as a consequence, an increase in the specific basal metabolism
Human DNA extraction from larvae: a brief review of the Literature: DOI: 10.5584/jiomics.v10i2.333
No full textThe analysis of the insects present on the corpses is a new frontier of Forensic Entomology Sciences useful for medico-legal evaluation, in order to extract human DNA and facilitate the estimation of the post-mortem interval.
Starting from a case of an unidentified and mummified body, colonized by insects at different developmental stages, we searched in the Literature the procedures of extraction and analysis of human DNA from the larvae. Our analysis found no trace of human genetic material in larvae’s puparia and crops.
This case report adds to the scarce literature available on the human DNA extraction from insects and highlights the analytical challenge of genetic analysis related to post-mortem tissue degradation
Proteomic Response to Arsenic Stress in Chromobacterium violaceum: DOI: 10.5584/jiomics.v2i1.84
No full textExposure to arsenic, whether acute or chronic, is a public health problem in many parts of the world and is associated with various types of deleterious effects on human health. One way that risk of contamination with this metalloid can be reduced is through bioremediation of areas contaminated with arsenic. Natural resistance mechanisms are widely distributed in microorganisms, meriting further study in an effort to improve their efficiency. Chromobacterium violaceum is a betaproteobacterium found in tropical and subtropical regions; its resistance to arsenic is controlled by an operon, arsRBC. The proteins expressed by the operon ars have been well studied; however, the overall cell response that determines resistance to this metalloid is little understood. We investigated changes in protein expression in response to arsenite. This was done through two-dimensional differential gel electrophoresis (2D-DIGE). Quantities of 26 proteins were altered after treatment with arsenite, 23 of which increased. The differential spots were analyzed with MS and MS/MS; eight proteins were identified that are involved in response to oxidative stress (SOD, GST, Grx), in DNA repair and in the metabolism of lipids, amino acids and coenzymes. We conclude that the response of C. violaceum to arsenite involves defense mechanisms against oxidative stress and alterations in cell metabolic cycles
