Journal of Integrated -OMICS
Not a member yet
215 research outputs found
Sort by
One Health Approach for Identification of Sources/Reservoir of Multidrug Resistant Bacteria in Wild Animals and their Environment: DOI: 10.5584/jiomics.v9i2.297
No full textBacteria such as extra-intestinal pathogenic E. coli (ExPEC) and methicillin-resistant S. aureus (MRSA) are important opportunistic pathogens. They might belong to pandemic, epidemic and/or sporadic clones. Some of the clones are associated with humans, others are associated with wild and/or domestic animals. Some clones are shared by both and may be found contaminating the environment. In these studies, we examined the spread of ExPEC from feces of Southern Resident Killer Whale (SRKW; Orcinus orca) that are associated with human diseases. We also examine MRSA isolates in wild rhesus macaques (Macaca mulatta), their environment and from humans. This One Health Approach aims to better understand the sources/reservoirs and possible transmissions of potential pathogens between animals, humans and their shared environment
The influence of reactive oxygen species and NO on oxidative metabolism and dielectric properties of living tissue: DOI: 10.5584/jiomics.v10i2.330
No full textThe aim of the work was a comprehensive assessment of the action of reactive oxygen species and nitric oxide on the scar tissue fragment ex vivo. The study was performed using fragments of scar tissue (n=10) removed intraoperatively in patients with Dupuytren's contracture. Each fragment was divided into 3 equal parts. The first part no manipulations were performed, the second one was treated with singlet oxygen, the third piece was processed with nitrogen oxide (20 ppm). The duration of the tissue treatment period was 5 minutes for all factors. Upon completion of the experiment in all samples, the methods of near-field resonance microwave sensing evaluated the dielectric properties of tissue using a software package developed at the Institute of applied physics of the Russian Academy of Sciences. Further, each portion of the tissue was homogenized using the apparatus "UltraTurrax" according to the standard procedure. The parameters of oxidative metabolism (intensity of free radical oxidation and total antioxidant activity) were studied in the obtained homogenates by Fe - induced biochemiluminescence. It was found that the treatment of scar tissue fragments by gas flow from singlet oxygen and nitric oxide generators leads to a change in the dielectric properties of the tissue and the intensity of free radical processes in it, and the nature of the response is specific to the influencing factor. It is shown that the peculiarity of the singlet oxygen action is a moderate increase in the dielectric permeability of the tissue and a balanced stimulating effect on the pro - and antioxidant systems. The NO effect at a concentration of 20 ppm is associated with a marked increase in dielectric permittivity and conductivity, as well as a significant increase in the antioxidant potential of the tissue
Multiparametric analysis of crystallogenic properties of blood serum of healthy people and patients with burn disease: DOI: 10.5584/jiomics.v10i2.329
No full textThe aim of the study was to assess some of the biophysical properties of dehydrated biological fluids in patients with thermal trauma. Own and initiated by the basic substance (0.9% sodium chloride solution) crystallization of blood serum of 32 patients with burn disease (contact burn, the area of skin lesions - 15-45% of the body surface; acute toxemia phase; age 21-40 years), as well as 30 healthy people of comparable age were studied. It is shown that in thermal injury undergo significant changes all outcome measures of biocrystalloscopic and impedance parameters in relation to their own and initiated the crystallogenesis of the blood serum. These changes are manifested in both time and quality parameters. Thus, burn disease in the stage of acute toxemia is characterized by slowing and inhibition of dehydration structuring of samples, increased degree of destruction of the formed elements, relative narrowing of the boundary zone, etc., which is confirmed by the data of registration of acoustic impedance. Based on these results, it is assumed that the visualization and study of the impedance characteristics of the dynamics of drying of serum droplets can be a convenient tool for diagnosing the patient's condition in combustiology
Special Issue: Selected Abstracts of the III International Caparica Conference in Splicing 2020: DOI: 10.5584/jiomics.v10i3.355
No full textSpecial Issue: Selected Abstracts of the III International Caparica Conference in Splicing 202
Electrophysiological and Proteomic Studies of Protobothrops mangshensis Venom Revealed Its High Bioactivities and Toxicities: DOI: 10.5584/jiomics.v2i2.114
No full textSnake venoms contain rich components having medical and biotechnological values. The proteomic characterization of snake venom proteome has thus potential benefits for basic research, clinical diagnosis, and development of new drugs for a variety of diseases. Protobothrops mangshensis is a monotypic genus of pit viper known only from Mountain Mang in Hunan Province of China, and represents the largest and the most spectacular snake among Asian venomous snakes. The venom of Protobothrops mangshensis exhibits a high coagulant activity on bovine and human fibrinogen and human plasma, a high phosphodiesterase activity and an arginine ester hydrolytic activity. In this study, the Protobothrops mangshensis venom was analyzed by 2D-gel electrophoresis separation, subsequently by in-gel digestion, MS/MS identification, and enzymatic activity analysis. Our results demonstrated that Protobothrops mangshensis venom comprised highly functional proteins and/or enzymes, and each of these proteins displayed multiple isoforms separated in 2-DE. Approximately 59.4% of the identified total 143 proteins had enzymatic activities and 24.5% were involved ion channels, representing highly complex and extensive bioactivities of the snake venom. The identified toxins included six protein families: serine proteinases, L-amino acid oxidase, phospholipases A2, C-type lectin-like proteins, cysteine-rich secretory proteins and metalloproteinase-disintegrin, and were correlated well with the clinical manifestations by Protobothrops mangshensis bite such as coagulopathy, oedema, hypotensive and tissue damaging effects. Electrophysiological studies showed that the snake venom inhibited tetrodotoxin-resistant (TTX-R) Na+ currents. All our results in this study provided the first functional proteomics of Protobothrops mangshensis venom
SPECIAL ISSUE: SELECTED ABSTRACTS OF THE IV INTERNATIONAL CAPARICA CONFERENCE ON URINE OMICS AND TRANSLATIONAL NEPHROLOGY 2019 (URINOMICS 2019) : DOI: 10.5584/jiomics.v9i3.315
No full textDOI: 10.5584/jiomics.v9i3.31
mRNA profiling in casework analyses: DOI: 10.5584/jiomics.v10i2.336
No full text
Our work involved the application of mRNA profiling to three bloodstains previously processed by the laboratory, in which routinely methods (immunochromatography) used for specific blood identification showed negative results.
Analyses were accomplished on a sweater worn by the suspect. No bloodstains were found during the routine inspection. In order to identify possible latent bloodstains, luminol was applied. After the reagent vaporization, three distinct areas of luminescence appeared. In order to confirm the presence of the human hemoglobin, luminescent surfaces were collected using 4N6FLOQSwabs® and tested using HemDirect Hemoglobin test (SERATEC®). All samples analysed showed negative results.
Due to the merging blood-specific markers mRNA profiling, the areas previously identified were sampled newly and tested using the three blood specific markers HBB, ALAS2 and CD93, together with two housekeeping genes represented by ACTB and 18S-rRNA.
All samples showed positive results for all three blood specific mRNA markers
Special Issue: Selected Abstracts of the II International Caparica Conference in Translational Forensics 2019: DOI: 10.5584/jiomics.v10i2.338
No full textSpecial Issue: Selected Abstracts of the II International Caparica Conference in Translational Forensics 201
Proteomic identification of muscle-associated biomarkers of amyotrophic lateral sclerosis using the wobbler mouse model of primary motor neuronopathy: DOI: 10.5584/jiomics.v4i2.171
No full text
Motor neuron disease is a major group of inherited or spontaneous disorders that are associated with muscular atrophy. Recently, muscle preparations from the genetic wobbler mouse model of primary motor neuronopathy have been analyzed by mass spectrometry-based proteomics. The progressive degeneration of individual motor neurons was shown to cause complex alterations in the concentration or isoform expression pattern of muscle proteins involved in the excitation-contraction-relaxation cycle, the cytoskeleton, ion handling, cellular signaling, the stress response and energy metabolism. In this article, we compare the panel of potential new muscle-associated biomarkers that have been obtained by two different, but complementary, bioanalytical approaches, i.e. label-free mass spectrometric analysis versus fluorescence two-dimensional difference-in gel electrophoresis. The complex disease-associated changes in the muscle proteome are considerably different to the more unilateral skeletal muscle transitions observed in experimentally denervated fibers or disuse-related muscular atrophy. The apparent subtype-specific vulnerability of neuromuscular synapses and compensatory mechanisms of fiber type shifting in motor neuron disease is discussed, and contrasted to other forms of muscular atrophy