Jurnal Riset Kimia
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Penentuan Kadar Karbohidrat pada Biji Cempedak Hutan (Artocarpus champeden Lour.) dengan Metoda Tembaga-Iodometri
Get PDFForest Cempedak Seeds (Artocarpus champeden Lour.) Are types of seeds that are underutilized by humans. Forest Cempedak Seed Flour has nutritional content of carbohydrates, fats, and proteins that can be processed as ingredients for food. This study aims to determine the carbohydrate content of Forest Cempedak Seeds (Artocarpus champeden Lour.) Conducted by the Copper-Iodometric method using a luff schoorl reagent. From the research that has been carried out obtained levels of carbohydrates in Cempedak Seeds (Artocarpus champeden Lour.) of 38.016%.Â
ANALISIS UJI INFUSA BUAH PETAI CINA, DAUN KEJI BELING DAN DAUN TEMPUYUNG SEBAGAI INHIBITOR ENZIM α-AMILASE DAN α-GLUKOSIDASE
Get PDFEnzim α-amilase dan α-glukosidase dalam proses pencernaan akan menghidrolisis amilum menjadi glukosa dan apabila glukosa darah melebihi batas normal (>140 mg/dL), maka seseorang didiagnosa menderita diabetes melitus. Pengobatan diabetes melitus khususnya tipe 2 biasanya diatasi menggunakan obat akarbose yang akan menginhibisi aktivitas α-amilase dan α-glukosidase. Pada penelitian ini akan dianalisis kemampuan inhibisi infusa dari sampel segar dan kering buah petai cina (Leucaena leucocephala L de Wit), daun keji beling (Strobilanthes crispus BI) dan daun tempuyung (Sonchus arvensis L.) terhadap ke 2 enzim ini. % inhibisi infusa terhadap aktivitas enzim α-amilase ditentukan menggunakan metode asam 3,5-dinitrosalisilat (DNS) sedangkan untuk α-glukosidase menggunakan substrat p-nitrofenil-α-D-glukopiranosida (p-NPG). Absorbansi hasil reaksi diukur menggunakan microplate reader pada panjang gelombang 530 nm untuk α-amilase dan 410 nm untuk α-glukosidase. Hasil penelitian menunjukkan bahwa % inhibisi infusa sampel kering lebih baik dibandingkan sampel segar dalam menghambat aktivitas enzim α-amilase dengan persentase sebagai berikut: infusa buah petai cina kering 92,54+1,11%, infusa daun keji beling kering 99,79+6,92% dan infusa daun tempuyung kering 87,63+3,95%, nilai ini tidak berbeda nyata dengan akarbose 93,89+ 0,02%. Sedangkan % inhibisi terhadap aktivitas enzim α-glukosidase dari semua sampel memiliki perbedaan yang nyata dengan akarbose (P<0,05) dengan nilai inhibisi 97,99+ 0,19%. Hasil ini menunjukkan bahwa ketiga tanaman tersebut berpotensi sebagai antidiabetes terutama dalam menginhibisi aktivitas enzim α-amilase
SINTESIS DAN KARAKTERISASI NUKLEOTIDA BERTANDA [α-32P]ATP
Get PDFThe utilization of nuclear technology in health sector with molecular techniques is increasingly developed today, especially in Indonesia. One of which is nucleotide compound marked with [α-32P]ATP, this compound has been used as tracer for deoxyribonucleic acid (DNA)/ ribonucleic acid (RNA) in the study of various physiological and pathological processes. [α-32P]ATP is synthesized through several stages of continuous reaction in one reaction vessel. It begins with synthesis of [γ-32P]ATP through an enzymatic reaction, using H332PO4 and ADP, and enzymes of lactate dehydrogenase, 3-phosphoglycerate phosphokinase and glyceraldehide 3-phospho dehydrogenase; followed by phosphorilation of 3’AMP with T4 polinucleotide kinase enzyme to produce 3’-[5’-32P]ATP. The result is hydrolyzed with nuclease P1 enzyme to produce [5’-32P]AMP. The unreacted [γ-32P] is degraded by the addition of hexokinase enzyme and glucose. At the final stage of the reaction, the [5’-32P]AMP is phosphorilated using phosphoenol-piruvat, piruvat kinase, and myokinase to produce [α-32P]ATP. The test results show that the every stage of reaction is characterized using TLC method, PEI cellulose paper as stationary phase and KH3PO4 0,5 M pH 3,5 as mobile phase. At the end of reaction, the yield of [α-32P]ATP reaches 71,7%, at Rf = 0,2
ANALISIS SENYAWA BERBAHAYA PARFUM ISI ULANG YANG DIJUAL DI KOTA PADANG MENGGUNAKAN METODE KROMATROGRAFI GAS-SPEKTROMETRI MASSA
Get PDFPerfume is widely used by most of people in this world. The increasing demand of perfume has caused many producers cheating by adding dangerous compounds and also unregistered by the National Agency of Drug and Food Control Indonesia. The aim of this study is to analyze the dangerous compounds in unregistered perfumes sold in Pasar Raya Padang City by using gas chromatography-mass spectrometry (GC-MS) method. In this Study, samples (A, B, C, D and E) were collected and the value of specific weight and refractive index of the samples were analyzed. The results showed that five samples contained relatively similar chemical compounds. Twelve compounds were detected and seven of them were harmful to health in refill perfumes which were dipropylene glycol, linalool, lily aldehyde, benzenepentanol, dimethylbenzyl carbinyl acetate, dihydro methyl jasmonate, alpha hexyl cinnamic aldehyde, based on Material Safety Data Sheet (MSDS). Regular monitoring of chemicals used in the manufacture of perfumes which may cause health risks to users should be controlled by National Agency of Drug and Food Control Indonesia
Kajian Kualitas Air Tanah Ditinjau dari Parameter pH, Nilai COD dan BOD pada Desa Teluk Nilap Kecamatan Kubu Babussalam Rokan Hilir Provinsi Riau
Get PDFBased on a preliminary study that has been carried out, Teluk Nilap Village, Kubu Babussalam District, Rokan Hilir was affected by the disposal of waste from oil company. The waste partly seeps into the residents' water sources which are used for domestic activities. It was because the location of the water sources close to the location of the waste oil disposal. The sampling technique in this study used the observation point technique (pumping test). Sampling refers to the point of waste oil flow (upstream, middle, downstream) and dug well water taken randomly (random sampling). The results showed pH values ranged from 2.8 to 6.6; BOD values range from 0.42 to 141.1 mg / L; COD values range from 20 to 291 mg / L. Ground water quality at some point in Teluk Nilap Village exceeded quality standard limits allowed and included in heavy pollution category
INHIBISI KOROSI BAJA RINGAN MENGGUNAKAN BAHAN ALAMI DALAM MEDIUM ASAM KLORIDA: Review
Get PDFThe corrosion of steel and its alloys is an important problem in industry, especially in acidic environments. Mild steel is one of the important alloys of iron which has many industrial applications because of its excellent mechanical properties, but mild steel is susceptible to corrosion. Corrosion problems need to be a concern and must be handled properly. Hydrochloric acid is widely used for pickling, cleansing, decomposition and metal etching, on the other hand also contributes to corrosion of metal surfaces. Plant extracts investigated the properties, mechanisms of adsorption and efficiency of inhibition as environmentally friendly corrosion inhibitors for various steel materials in different acidic media. The efficiency of corrosion inhibition of steel from plant extracts analyzed ranged from 72-98% and generally as a mixed-type inhibitor. Most of the inhibitors are adsorbed on the steel surface through a physisorption mechanism
KAJIAN TEORITIS KEMAMPUAN CAPPING KATEKIN, KATEKU TANAT DAN QUARSETIN TERHADAP NANOPARTIKEL PERAK DENGAN MENGGUNAKAN METODA DFT-B
Get PDFInterdiffusion mechanism of catechin, catechutannic acid and quercetin are studied by using DFT-B method. But before conducting these experiment, we perform molecular dynamic simulations to find adsorption models probability of each compound. Two models adsorption of quarcetin, four models for catechin and three models for catechutannic acid have been obtained from molecular dynamic simulations. Quantum parameter of each compound, energy and properties adsorption models have been calculated and discussed. The theoretical results were found to be consistent with the experimental data reported. Futhermore, optimization adsorption model samples show that all of adsorbents have weak bonds on the surface of silver nanoparticles. The mechanism can be classified as strong physisorption, so that catechin, catechutannic acid and quercetin can be categorized as good capping agent in synthesis of silver nanoparticles
SINTESIS LAPISAN TIPIS TiO2 BERPORI YANG DIMODIFIKASI OLEH NITROGEN DENGAN METODE PEROKSO SOL-GEL, KARAKTERISASI DAN APLIKASINYA SEBAGAI MATERIAL PEMBERSIH DIRI (SELF CLEANING MATERIAL)
Get PDFThe photocatalytic properties of TiO2 compound in anatase phase can be activated under visible light by nitrogen modification and its ability can be increased by generated porous structure using polietilen glikol (PEG) on TiO2 thin film surface. The porous N/TiO2 thin films were prepared by heating aqueous peroxotitanate thin films by addition of polietilen glikol (PEG) deposited uniformly on superhydrophilic uncoated glass at 500 oC for 1 h. The result of X-ray diffraction (XRD) confirmed the resence of only anatase phase for all samples. The UV-Vis spectroscopy showed the synthesized porous N/TiO2 thin films exhibit the absorption in the visible range (400-500 nm). Photocatalytic activity of porous N/ TiO2 thin films were evaluated by using fourier transform-infrared spectroscopy (FTIR) to determine the ability of this photocatalyst for stearic acid degradation under visible light irradiation. The result showed that N/TiO2/PEG-2.1 thin film degraded the stearic acid was about 87,86%, which was 1,12 times higher than that of N-doped TiO2 and 9,9 times higher than that of undoped TiO2 thin film