HAYATI Journal of Biosciences
Not a member yet
1069 research outputs found
Sort by
Naringin Effect on SARS-CoV-2 Pseudovirus Entry and Spike Mediated Syncytia Formation in hACE2-overexpressing Cells
Get PDFA molecular docking study demonstrates the interaction between naringin, a citrus flavonoid, with SARS-CoV-2 spike RBD. Nevertheless, in vitro investigation of the inhibitory effect of naringin on SARS-CoV-2 entry and syncytia models has yet to be carried out. We synthesized VSV∆G-GFP/Spike* pseudovirus (PSV) as a SARS-CoV-2 model by pseudotyping VSV∆G-GFP/S* in BHK-21 cells overexpressing the SARS-CoV-2 spike glycoprotein. In the SARS-CoV-2 PSV entry assay, we utilized CHO-K1 cells transfected with hACE2 plasmid, which were then treated with naringin and SARS-CoV-2 PSV/naringin. After 16-18 h incubation, PSV internalization represented by the GFP signal was observed under a fluorescence microscope. Immunofluorescence staining was also performed to probe the SARS-CoV-2 spike and confirm the PSV entry. We performed a syncytia assay using 293T cells co-transfected with SARS-CoV-2 spike/hACE2. Six hours after transfection, the cells were treated with naringin and incubated for another 16-18 hours. Then, we observed syncytia using a phase contrast microscope. Based on fluorescence foci quantification, the results indicated that naringin might inhibit SARS-CoV-2 PSV entry at a concentration of 100 µM (P<0.05). However, naringin did not prevent syncytia formation compared to solvent control. These PSV entry and syncytia assay results suggested that naringin potentially inhibited SARS-CoV-2 viral infection but not cell-to-cell viral transmission
Cloning and Production of Antigen 85A Mycobacterium tuberculosis for Diagnostic Latent Tuberculosis: a Preliminary Study
Get PDFThe main challenge in the management of Tuberculosis (TB) is diagnosing quickly and accurately, especially Latent Tuberculosis Infection (LTBI). LTBI detection was carried out using the Tuberculin Skin Test (TST) and Interferon Gamma Release Assay (IGRA). In TB endemic areas, these two examinations have limitations, so current research is directed at finding specific antigens for the diagnosis of LTBI. One of the potential proteins is Antigen 85A (Ag85A) Mycobacterium tuberculosis (Mtb) encoded by Fibronectin-binding protein A (FbpA). The Ag85 complex induces the proliferation of T-cells and interferon-gamma in most healthy individuals infected with Mycobacterium tuberculosis, Mycobacterium leprae, and BCG-vaccinated mice, making it a potential antigen. This study aims to clone and produce recombinant protein Ag85A from Mtb in Escherichia coli BL21. The methods used were ligation to the pET-32a expression vector, transformation to Escherichia coli BL21, and production of protein by IPTG induction. Characterization of recombinant clones by colony PCR and sequencing. The results obtained were that the fbpA gene isolated from Mtb clinical isolate had been amplified, and the PCR product was 900 bp. The production of Antigen 85A has been successfully carried out and produces 44 kDa
Phylogenetic Analysis of Cyclocheilichthys apogon and Cyclocheilichthys armatus (Fish: Cyprinidae) from West Sumatra
Get PDFCyclocheilichthys apogon (Beardless Barb fish) and Cyclocheilichthys armatus (Hihgfin Barb fish) in West Sumatra is known by the local name Turiak and Catua fish, respectively, are economically important species. Reducing populations and limited biological information about these fishes make their genetic diversity and phylogenetic relationships crucial. The genetic variation was investigated by analyzing the sequencing of a part of mitochondrial DNA Cytochrome b (Cyt b) and Cytochrome oxidase-I (COI) genes of 23 individuals collected from West Sumatra populations. Phylogenetic analysis revealed the monophyletic lineage of the Cyclocheilichthys group and supported Anematichthys as the synonym of Cyclocheilichthys. C. armatus was closely related to C. janthochir and separated from C. apogon with sequences divergence were 12.7-14.0% (Cyt b) and 9.4%-10.0% (COI). C. apogon and C. armatus have low haplotype diversity and genetic diversity, respectively. Genetic diversity within and among populations with sequences divergence was 0.0-0.8% (Cyt b) and 0.0%-0.7% (COI), respectively. The low-level diversity may signify a possibility of gene flow within and among populations. The current data suggests all populations as a single stock unit. Present genetic information is important to establishing effective population management, conservation, and breeding strategies for Turiak and Catua fish
Activity Budget and Diet in Sumatran Orangutan (Pongo abelii) at Soraya Research Station, Aceh
Get PDFGeographical and environmental factors influence the food availability and diet of orangutans. Moreover, tree phenology affects productivity and food availability in orangutan habitats. As a result, orangutans must be able to adapt to their food availability in the wild. This study aims to analyse the daily activity patterns and eating behaviour of the Sumatran orangutan (Pongo abelii) and their relationship to food availability through the fruit availability index (FAI) and phenology at Soraya Research Station (SRS), Aceh. From the 648 hours of observation on five orangutans\u27 daily activities, we found that resting is the activity with the highest percentage (48%), followed by feeding (22.5%) and moving (21.8%). The high percentage of resting activity might correlate with its low FAI in SRS (1.01%). The resting activity limits orangutans\u27 energy expenditure. Furthermore, barks, fruits, and other plants (including lianas, ferns, and epiphytes) were the main type of diet of Sumatran orangutans in SRS with a frequency of 40.6%, 28.9%, and 15.6%, respectively. The high bark consumption by the orangutans acts as their fallback diet at SRS and is thought to be a form of adaptation to the low fruit availability. This study showed the orangutan adaptation to low fruit availability in SRS through their daily activity and diet patterns
Genetic Diversity and Population Structure Analysis of Potato Somaclones
Get PDFCultivated potato varieties are tetraploid and vegetatively propagated. Therefore genetic improvement for new traits is less effective through hybridization. The mutation is considered an alternative method for crop improvement of those varieties. This study aimed to evaluate the effects of gamma-ray irradiation on increasing genetic diversity among the somaclones and the changes in population structure. Forty-four somaclones were used as observed materials. The population was grown in a screen house using a completely randomized design with genotype (somaclone) as a single factor. Thirteen qualitatively and quantitatively morphological characters were observed. Six SSR markers were used for analyzing the population structure. The selection process was based on a weighting method divided into quartiles. Selected numbers were taken from quartile 3. Morphological analysis of stems and leaves resulted in five different somaclones, with significant differences in anthocyanin appearance and intensity of the green color of the leaflets. Diversity criteria based on quantitative characteristics showed a high level of diversity with a high heritability for tuber weight, length, and diameter and a moderate heritability for tuber number. Analyzing the population structure has offered insight into how gamma irradiation affected the somaclones. As a result of the selection, 12 somaclones met the requirements to serve as seed sources for field testing. It is expected that the results of this study will provide information about the diversity caused by gamma-ray irradiation treatment on potato somaclones and a method for improving the efficiency of the initial selection of potato populations
Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions
Get PDFFish bodies respond to changes in environmental conditions in various ways and degrees. Seven fish species were collected from three study locations, including two nearshore sites (Ha Tinh province and Nha Trang city, Khanh Hoa province) and one offshore site (Toc Tan island, Khanh Hoa province) to evaluate alterations in erythrocyte morphology under certain environmental conditions. Heavy metal concentrations at all study sites were analyzed and met the acceptable technical criteria for each site. Fish blood samples were taken from the tail veins, anticoagulated with heparin, and stained with Giemsa stain. The blood cell morphological study was performed using an optical microscope. The results revealed two types of morphological abnormalities of fish erythrocytes, including nuclear deformation and nuclear-matter distribution
Antioxidant Properties of Active Fraction Extract Derived from Yellow-Red Pigment Produced by the Marine Sponge-Associated Bacterium Bacillus haikouensis AGS112 and Identification of Related Compounds
Get PDFSponge-associated bacteria can produce bioactive compounds similar to the host. Here, the investigation of antioxidant properties of the yellow-red pigment produced by sponge-associated bacterium AGS112 was conducted using in vitro and in vivo analysis. The 16S rRNA gene sequence showed the sponge-associated bacterium AGS112 has the closest similarity with Bacillus haikouensis C-89 (99%). The crude pigment extract produced by Bacillus haikouensis AGS112 contained both phenolic and flavonoid. The peaks occurred at 412 nm and 664 nm, which indicated as the presence of carotenoids and flavonoids, respectively. The active fraction extract obtained through bio-autography TLC, and had more potent antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) compared to the crude pigment extract with an IC50 value of 68.62±0.59 µg/ml and 198.88±1.66 µg/ml, respectively. Moreover, the active fraction extract at the concentration of 35 µg/ml could better enhance the viability of Schizosaccharomyces pombe and achieve the highest expression of sod1 and ctt1 genes. Metabolite profiling using LC-MS analysis confirmed the active fraction extract contains eudesmin and artelastin that might be contributed as antioxidants. These results suggest that the active fraction extract of the yellow-red pigment produced by B. haikouensis AGS112 had potency to be used as candidate for natural antioxidants
The Potential of Porang (Amorphophallus muelleri Blume) Flour and Porang Flour Formulation as an Anti-Diabetes Type-2 Agent
Get PDFPorang (Amorphophallus muelleri Blume) is a source of glucomannan in Indonesia, and a potential agent for diabetes mellitus treatment, apart from konjac. This study aims to determine the potential of porang flour formulation as a therapeutic agent for diabetes mellitus, especially type-2 diabetes mellitus. Soluble fiber, water absorption capacity(WAC), disintegration time, and viscosity of porang flour (PF) and porang flour formulation (PFF: consist of 85% porang flour, 1.03% k-carrageenan flour, 12% inulin flour, and 1.97% modified cassava flour (MOCAF)) were scrutinized. Twenty-eight male albino Wistar rats were randomly split into seven experimental groups. Five groups consisting of diabetic rats were treated using porang flour (300 mg/kg bw); low, middle, and high doses of porang flour formulation (100, 300, 500 mg/kg bw, respectively); and metformin (51.38 mg/kg bw). The rest were normal, and the diabetic (DM) control group. PF, PFF, and metformin were orally administered to the streptozotocin-induced diabetic rats per day for four weeks of the experiment. Fasting plasma glucose (FPG), Malondialdehyde (MDA) level, lipid profile, aspartate aminotransferase (AST), and alanine transaminase (ALT) levels of the blood plasma were measured, while the pancreas was used for immunohistochemical study and β-cells quantification. ANOVA was employed to analyze the data, followed by Honestly Significance Difference using Minitab version 17.0. The result indicated a significant effect of PF, PFF, and metformin on decreasing FPG and MDA and increasing the number of pancreatic β- cells in DM rats. Porang flour (300 mg/kg bw) and middle-dose PFF are potential therapeutic agents for type-2 DM
Xylem Vessels Traits of Oil Palm Roots Influenced by Root Diameter and Soil Hydrological Regime
Get PDFOil palm has been widely studied regarding growth and development, water use, productivity, and other economically relevant functions. However, not much is known about the hydraulic conductivity of oil palm root systems and how xylem vessels perform their function to transport water from roots to shoots so far. This information is needed to describe oil palm strategies to maintain water status, especially in oil palms that grow under various soil hydrological regimes. To investigate the root hydraulic performance, we measured mean xylem vessel diameter (D), vessel density (VD), vessel lumen area (Alumen), and potential hydraulic conductivity (Kp) for oil palm root samples in seasonally flooded riparian and well-drained sites at Harapan Jambi Forest. The result showed that D, Alumen, and Kp increased with increasing root diameter at both plantation types. On the contrary, VD significantly decreased with increasing root diameter. Potential hydraulic conductivity (Kp) in riparian sites was smaller than in well-drained sites and significantly different in root diameter >2-5 and >5-10 mm and related to both plantation types. The low potential hydraulic conductivity of root xylem vessels and the narrowing of xylem vessel lumen that occurs in oil palm roots in the seasonally flooded riparian sites were presumed as adaptation mechanisms to maintain water supply from the roots to the shoot in oil palm plants in these sites
Isolation, Identification and Assessment of Efficient Cellulase Producing Bacteria from the Termite Guts
Get PDFThe present study is concerned with the screening the cellulase-producing bacteria from termite gut, assessed potential cellulase-producing bacteria and partial characterization (optimum parameters) of cellulase from isolated bacteria. The result showed that 15 out of 48 isolated strains was positive for degrading the carboxy methyl cellulose (CMC) in agar by congo-red method. After screening by DNS assay, three selected bacteria exhibited high cellulase activity that were identified as Citrobacter amalonaticus CM 1-3, Bacillus cereus CM 5-1 and Streptococcus salivarius CE 5-1 using 16S rRNA sequence analysis. All bacterial strains utilized CMC and showed the highest cellulase activity. Cellulase characterization of C. amalonaticus CM 1-3 and S. salivarius CE 5-1 was revealed optimum activity at 35°C, pH 7.0 and for 48 h. Bacillus cereus CM 5-1 represented its potential use in industrial processes due to thermostable cellulase production. The crude cellulase of this strain was purified by (NH4)2SO4 precipitation with 1.58 purification fold and 74.38% overall recovery. The optimal temperature and pH for cellulase activity of B. cereus CM 5-1 were at 40°C and pH 7.0. Thus, this study provided additional information about the diversity and partial characteristic cellulase of cellulolytic bacteria from termite gut for future industrial applications