HAYATI Journal of Biosciences
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Application of Lactic Acid Bacteria to Produce Bioactive Compounds from Tofu Waste Using Pineapple Bromelain Enzyme
No full textTofu is one of the main human foodstuffs and has become an alternative substitute for animal protein. Tofu contains relatively high protein, and active compounds, but the waste produced during the tofu processing process is very abundant, namely 3.5 million tons per year in Indonesia. The production of active compounds from tofu waste can use bromelain enzymes. The bromelain enzyme in this study was produced from the fermentation of pineapple leaf waste using Pediococcus pentosaceus E7. The production of active compounds from tofu waste has not been widely reported, so this study aimed to produce active compounds from tofu waste. This study began with bromelain production, measurement of bromelain activity, bromelain precipitation, peptide production from tofu waste, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and antibacterial testing of active compounds derived from tofu waste degradation. The results showed that the peptide content of tofu liquid waste degraded using the bromelain enzyme was 41.01±0.06 mg/mL. The LCMS results showed that 18 bioactive compounds were contained in the degradation products of tofu liquid waste. The active compounds derived from the degradation of tofu waste are capable of inhibiting the growth of Salmonella Typhimurium ATCC 14028, yielding a clear zone index of 2.53±0.12
Effectiveness of Earthworm (Lumbricus rubellus Hoffmeister) and Cinnamon (Cinnamomum burmannii (Nees and T. Nees) Blume) Combination Capsule as Herbal Preparation in Reducing Cholesterol and Blood Sugar Levels
Full text linkCardiovascular diseases, including coronary heart disease and heart attack symptoms, are the leading causes of death worldwide. High cholesterol levels and blood sugar levels contribute significantly to these diseases. Therefore, this study aimed to determine the effectiveness of combining capsules of earthworm (Lumbricus rubellus) and cinnamon (Cinnamomum burmannii) in lowering cholesterol and blood sugar levels. After conducting pre-clinical trials, clinical trials were further carried out in humans. Cholesterol and blood sugar levels were measured for 14 days in Phases 1 and 2. Statistical data analysis was conducted using the Shapiro-Wilk normality test, paired t-hypothesis test, Wilcoxon test, and frequency analysis. The results showed that in Phase 1, there were significant differences in volunteers\u27 cholesterol and blood sugar levels with no clinical symptoms. The most significant decrease in cholesterol levels in female volunteers occurred at the age of 21-30 years, at 25.56%, while the most significant reduction in blood sugar levels occurred at 41-50 years, at 41.50%. On the other hand, the most significant decrease in cholesterol levels in male volunteers occurred at the age of 41-50 years at 33.08%, and the most significant reduction in blood sugar levels occurred at the age of >60 years at 38.87%. The most significant content in the combination capsule was fatty acids and cinnamaldehyde. In conclusion, consuming a combination of earthworm and cinnamon capsules for 14 days caused a reduction in cholesterol and blood sugar levels
Comparative Analysis of Bioactive Compounds in Invasive and Native Land Snail Species of Java, Indonesia
Full text linkLand snails are part of Indonesia\u27s rich biodiversity. Their mucus is widely used as a natural ingredient in pharmaceuticals and cosmetics. Despite this, research has primarily focused on a limited range of species, leaving the potential of many native land snail species unexplored. This indicates that there is still significant untapped potential in native Indonesian land snail species. This research aims to analyze the bioactive compounds in the mucus of native Indonesian land snails and compare them with those of invasive species. The research employed Liquid Chromatography-Mass Spectrometry (LC-MS) to identify biologically active substances in land snail mucus. The samples used were Amphidromus palaceus and Lissachatina fulica collected from Gunungkelir, Yogyakarta, and Amphidromus perversus from Gunungkidul, Yogyakarta, and Jember, East Java. The results revealed that A. palaceus exhibited the highest number of bioactive compounds, with 28 identified substances. The bioactive properties across all samples were categorized as antibacterial (16%), wound healing and anti-inflammatory (12%), anticancer (8%), neuron-related drugs (6%), anti-aging (4%), with the remaining activities related to liver-related drugs, antiviral, heart disease-related drugs, and kidney disease-related drugs. These results highlight that native species have significant potential for further research, enabling their application in medicine and cosmetics
Integrated DNA Barcoding and Morphometric Characterization of Palm Weevils (Rhynchophorus spp.) in North Sulawesi
Full text linkPalm weevils (Rhynchophorus spp.) are significant pests of sago palms worldwide. Yet, the taxonomy and evolutionary lineage of these species in North Sulawesi remain unclear, likely due to geographic isolation driving genetic variation and species differentiation. This study aimed to investigate the genetic diversity and morphological differentiation of Rhynchophorus across distinct geographic regions in North Sulawesi using an integrative approach combining DNA barcoding and morphometric analysis. Morphometric traits from palm weevil specimens collected in Sangihe Island, Minahasa, and Bolaang Mongondow were measured and statistically analyzed using one-way ANOVA, MANOVA, principal component analysis (PCA), and linear discriminant analysis (LDA) to assess interpopulation morphological differences. For molecular identification, the cytochrome oxidase I (COI) gene was amplified and sequenced. Phylogenetic relationships were inferred using the maximum likelihood method, and a DNA barcode gap analysis was conducted to evaluate the separation between intra- and interspecific genetic distances. Morphometric analysis revealed significant size variations among the specimens, particularly in rostrum dimensions, with the EBBM (Bolaang Mongondow) group showing the highest values. COI-gene-based identification confirmed that all specimens were of R. vulneratus. However, phylogenetic analysis showed EBMin (Minahasa) and EBBM forming a distinct subgroup, while EBMan, EBSTS, EBTam, and EBSTU (all from Sangihe Island) clustered separately. Barcode gap analysis demonstrated a clear distinction between intra- and interspecific divergence, validating COI as a reliable marker for species delimitation. This study concludes that integrating morphometric and genetic analyses reveals geographic structuring within R. vulneratus, highlighting the effectiveness of combined methods for accurate identification and population differentiation
Exploring Polycarpa aurata Quoy and Gaimard, 1834 Extracts as Antibiotic Candidates: GC-MS Profiling and Molecular Docking Study
Full text linkMarine natural products have garnered global interest due to their remarkable bioactive compounds. The tunicate P. aurata, a highly abundant marine invertebrate, possesses significant bioactive potential with applications as an anti-cancer, antibacterial, and antifungal agent. This study aims to evaluate the potential of P. aurata bioactive compounds as antibiotic candidates through in vitro testing and to explore their activity via an in-silico approach using molecular docking. P. aurata was extracted using maceration, yielding both methanol and n-hexane extracts. The methanol extract of P. aurata demonstrated greater efficacy than the n-hexane extract, particularly against Staphylococcus aureus with an inhibition zone diameter of 18.8 mm, compared to 13 mm for Salmonella typhi, both at a 25% concentration. In comparison, the positive control, ciprofloxacin, produced an inhibition zone ranging from 22 to 24 mm for both bacterial strains. GC-MS analysis of the extract revealed three compounds with high % area and similarity index values: Cyclohexane, 1,3,5-triphenyl, Cholesta-5,22-dien-3-ol, and Cholesta-3,5-diene, all of which were suitable for the selected protein target. Computational analysis through molecular docking demonstrated that these compounds exhibit stronger binding affinities compared to ciprofloxacin. This study suggests that the extract of P. aurata is a promising source of bioactive compounds with substantial therapeutic potential as an antibacterial and antibiotic candidate
Intermittent Hypobaric Hypoxia Preconditioning Ameliorates Kidney Damage Compared to Acute Hypoxia
Full text linkMany studies have reported that intermittent hypobaric hypoxia (IHH) can cause oxidative stress and tissue damage in the kidneys, which can lead to impaired kidney function. However, a study using simulated intermittent hypobaric hypoxia was considered to have a protective role for the kidneys. This study aims to determine the effect of IHH on kidney adaptation to oxidative stress and kidney damage. Twenty-five rats were divided into five groups: control, one-time hypoxia (AHH), two-time hypoxia (IHH 1), three-time hypoxia (IHH 2), and four-time hypoxia (IHH 3). The intermittent interval was one week, and at the time of treatment, the rats were placed in a hypoxic chamber. At the end of treatment, the rats were sacrificed, and the kidneys, urine, and blood were collected. The kidney tissues were used for protein assay, ELISA (HIF-1α and VEGF), qPCR (cytoglobin (Cygb) and renin), SOD and GPx activity assay. Blood was used for creatinine and urea assay, and urine was used for KIM-1 ELISA. Formalin-submerged tissues were used for histopathological analysis. The level of HIF-1α and VEGF increased significantly from AHH to IHH 1. Cygb and renin expressions peaked at AHH and decreased at subsequent IHH groups. The Nrf2 level and GPx activity didn’t show any difference, but SOD activity peaked at IHH 1. The creatinine level only peaked at IHH 2; other groups remained the same as the control. Urea levels decreased with more IHH sessions, and KIM-1 didn’t show any difference. Our findings exhibit that hypoxia preconditioning by IHH treatment leads to the kidney’s adaptability to hypoxia and does not cause kidney damage
Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential
Full text linkThe rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively
Antibacterial Activities of Red Mangrove (Rhizophora stylosa Griff.) Leaf Extract against Klebsiella pneumoniae ATCC 700603
Full text linkMultidrug-resistant (MDR) Klebsiella pneumoniaeis a critical pathogen causing severe human diseases, including pneumonia. Combating the growing threat of MDRK. pneumoniae requires innovative approaches, such as exploring plant-derived antibacterial agents. Rhizophora stylosa Griff., a mangrove species with traditional medicinal uses, is recognized for its bioactive compounds with potential antibacterial properties. However, research on its bioactive constituents remains limited. This study investigated the antibacterial activity of R. stylosa leaf extracts prepared via maceration and liquid-liquid fractionation against K. pneumoniae ATCC 700603. Using agar-well diffusion and cell leakage assays, the water fraction demonstrated moderate inhibition of K. pneumoniae, producing an average inhibition zone of 8.24 mm and a minimum inhibitory concentration (MIC) of 400 mg/ml. UV-Vis spectrophotometry revealed that the water fraction disrupted protein and nucleic acid synthesis, evidenced by leakage of cellular materials at 260 and 280 nm. Additionally, scanning electron microscopy (SEM) images of K. pneumoniae cells treated with the water fraction showed pore formation and structural damage. These results emphasize the antibacterial potential of the water fraction of R. stylosa leaves against MDRK. pneumoniae. Further investigations are necessary to isolate and identify the specific bioactive compounds responsible for these effects. Moreover, comprehensive assessments of activity and toxicity are crucial to advance R. stylosa-based antibacterial agents as promising alternatives for addressing the challenge of MDR bacterial infections
High-Resolution Microscopic Images of Mosquito Vectors (Diptera: Culicidae): Proper Identification of Culex and Aedes during Dengue Outbreak Situation in Bangladesh
Full text linkHigh-resolution microscopic images of the 8th abdominal segment, comb scales, pectin, tuft of hairs, saddle, head antenna, thoracic hooks and other morphological characteristics of Culex quinquefasciatus, Aedes aegypti and Aedes albopictus have been provided for the first time in Bangladesh. Mosquitoes spread deadly diseases (dengue, chikungunya, Japanese encephalitis, yellow fever, malaria, filariasis) that kill millions of people every year including Bangladesh. During dengue and dengue outbreak situations, it is very much necessary to identify the dengue-occurring mosquito vectors. Proper identification of the mosquito vectors of Bangladesh is the key factor for public health protection and disease prevention. Larval movement patterns and habitat characterization were also observed. Results obtained in this study revealed that dengue virus can also be carried by other Aedes species rather than A. aegypti. Results were discussed with previous study dealing with Aedes mosquito samples of Bangladesh. The findings will be very useful for the proper identification of dengue mosquito vectors in Bangladesh and the knowledge assists in the accuracy of mosquito control activities as well as prevention of mosquito-borne diseases
Modification of the Kato-Katz Diagnosis Technique Using “Canang” Flower Waste Extract as a Staining for Ascaris lumbricoides Eggs
No full textHelminthiasis, particularly that caused by Ascaris lumbricoides, is a major global health issue, especially in areas with poor sanitation. One method for preventing and controlling Ascaris lumbricoides infection is through identification via the Kato-Katz diagnostic technique. The use of synthetic dyes, such as methylene blue, in the Kato-Katz method raises concerns for both human health and the environment. In humans, methylene blue causes skin irritation, gastrointestinal issues upon ingestion, and systemic effects. Furthermore, its environmental impact includes reducing light penetration and acting as a toxic component in food chains. An alternative approach involves utilizing post-use offerings from Hindu rituals in Bali, known as canang, which consist of flower components such as Impatiens balsamina L. and Tagetes erecta. The natural dyes found in these flowers serve as an alternative to traditional staining methods. This study examined the efficacy of flower extracts as stainings using the cellophane absorption test, helminth egg detection and morphology identification, pH test, and measurement of heavy metal concentration. The dye made from canang flower waste at 3% did not differ much from manufactured stainings. Thus, canang flower waste is a safe alternative